Deletion in HSP110 T₁₇: correlation with wild-type HSP110 expression and prognostic significance in microsatellite-unstable advanced gastric cancers

Kim, K.J.,Lee, T.H.,Kim, J.H.,Cho, N.Y.,Kim, W.H.,Kang, G.H. W. B. Saunders Co ; Centrum Philadelphia 2017 Human pathology Vol.67 No.-

<P>Deletion of the HSP110 T-17 mononucleotide repeat has recently been identified as a prognostic marker that is correlated with wild-type HSP110 (HSP110wt) expression in microsatellite instability-high (MSI-H) colorectal cancers. The aim of this study was to assess the correlation between deletion of the HSP110 T-17 repeat and expression of HSP110wt using DNA testing and immunohistochemistry and to determine the prognostic implications of HSP110 T-17 deletion in MSI-H advanced gastric cancers (GCs). The status of HSP110wt expression was evaluated by immunohistochemistry using an HSP110wt-specific antibody in 142 MSI-H advanced GCs. The size of the HSP110 T-17 repeat deletion was analyzed in 96 MSI-H advanced GCs; deletions were divided into small (0-2 base pairs) and large deletions (3-5 base pairs). Low and high expressions of HSP110wt were detected in 38 (26.8%) and 104 (73.2%) of the 142 cases, respectively. The HSP110 T-17 deletion was observed in 45 (46.9%) of the 96 MSI-H GC samples. Tumors with high expression of HSP110wt showed a tendency to have small or no deletion of HSP110 T-17. In Kaplan-Meier survival analysis, tumors with a large HSP110 T-17 deletion were associated with favorable overall survival and disease-free survival compared with those with small/no deletion of HSP110 T-17. However, HSP110 T-17 deletion size was not an independent prognostic factor in multivariate analysis. In summary, deletion of the HSP110 T-17 repeat was frequently observed in MSI-H GCs, and HSP110 T-17 deletion size was inversely correlated with HSP110wt expression status. Large HSP110 T-17 was not a prognostic indicator in MSI-H GCs. (C) 2017 Elsevier Inc. All rights reserved.</P>

Programmed cell death ligand 1 alleviates psoriatic inflammation by suppressing IL-17A production from programmed cell death 1-high T cells

Kim, J.H.,Choi, Y.J.,Lee, B.H.,Song, M.Y.,Ban, C.Y.,Kim, J.,Park, J.,Kim, S.E.,Kim, T.G.,Park, S.H.,Kim, H.P.,Sung, Y.C.,Kim, S.C.,Shin, E.C. Mosby 2016 The journal of allergy and clinical immunology Vol.137 No.5

<P>Background: Psoriasis is one of the most common chronic inflammatory diseases of the skin. Recently, IL-17-producing T cells have been shown to play a critical role in psoriatic inflammation. Programmed cell death 1 (PD-1) is a coinhibitory receptor expressed on T cells in various chronic inflammatory diseases; however, the expression and function of PD-1 during psoriatic inflammation have not previously been characterized. Objective: We examined PD-1 expression on IL-17A-producing T cells from imiquimod-treated mice and patients with psoriasis. Additionally, we investigated the therapeutic effect of recombinant programmed cell death ligand 1 (PD-L1) protein on imiquimod-induced psoriatic inflammation. Methods: PD-1 expression on IL-17A-producing gamma delta T cells from imiquimod-treated mice was examined by means of multicolor flow cytometric analysis. In the psoriatic skin of patients, PD-1 and IL-17A expression was analyzed by using immunofluorescence. The therapeutic effect of PD-L1-Fc fusion protein (PD-L1-Fc) was assessed in imiquimod-treated mice ex vivo and in vivo. Results: During imiquimod-induced psoriatic inflammation, PD-1 is overexpressed on CD27(-)V gamma 1(-) gamma delta T cells. Furthermore, PD-1 expression on IL-17A(+) T cells was confirmed in psoriatic skin tissues from patients and imiquimod-treated mice. In the CD27(-)V gamma 1(-) gamma delta T-cell population, V gamma 4(-) gamma delta T cells with V gamma 6 mRNA expression showed a high level of PD-1 expression. Furthermore, these PD-1(hi)V gamma 4(-)(V gamma 6(+)) gamma delta Tcells were specialized for anti-CD3-induced IL-17A production, which was inhibited by PD-L1-Fc treatment. In imiquimod-treated mice PD-L1-Fc reduced psoriatic inflammation when given alone and enhanced the therapeutic effect of anti-p40 when given in combination. Conclusion: PD-1 is overexpressed in IL-17A-producing T cells in both imiquimod-treated mice and patients with psoriasis. Moreover, recombinant PD-L1-Fc alleviates psoriatic inflammation in imiquimod-treated mice.</P>

정상 임신에서 임신주기에 따른 갑상선기능의 변화

김원배,정재훈,윤보현,이석인,김민선,오태근,조보연,이홍규,고창순 대한내분비학회 1994 Endocrinology and metabolism Vol.9 No.3

It is well known that normal pregnancy is accompanied by a rise in serum concentrations of thyroxine-binding globulin(TBG) and human chorionic gonadotropin (hCG). Alterations of biochemical parameters of thyroid function are recognized during gestation and sensitive tests to evaluate the alterations easily are required. Therefore, a cross-sectional study was undertaken in 140 healthy pregnant women to evaluate the efficacy of free T_4 measured by 2-step RIA compared to other thyroid function tests and to confirm the changes of thyroid function according to the stages of normal pregnancy. The sensitivities of free T_4 index, free T_4(by 2-step RIA), T_3 and TSH were realtively high(99.3%, 93.6%, 92.9%, 83.6%, respectively) compared to those of T_4 and T_3 bead upgake(49.3%, 21.4%) during all stages of pregnancy. There were positive correlations between free T_4 index and free T_4 or total T_4(r=0.68, r=0.72; p$lt;0.001). The values of free T_4 index sharply decreased from 3.22+-0.10(meam +-SEM) during 6th-12th week to an plateau after 16th-20th week of gestation(p$lt;0.01). The serum concentrations free T_4 and T_3 bead uptake also significantly decreased from 1.65+-0.05 ng/dl, 24.7+- 0.7% during 6th-12th week to an plateau after 16th-20th week of gestation, respectively(p$lt;0.001), No differences were found in the changes of serum concentrations of T_3, T_4 and TSH according to the stages of pregnancy. In conclusion, it is adequate to measure some tests including free T_4 index and free T_4 to evaluate thyroid function during pregnancy. The thyroid physiology and changes of thyroid function according to the stages of pregnancy should be considered in the interpretation of thyroid function status during pregnancy(J Kor Soc Endocrinol 9: 183-189, 1994).

Bacterial community analyses by pyrosequencing in dark fermentative H₂-producing reactor using organic wastes as a feedstock

Im, W.T.,Kim, D.H.,Kim, K.H.,Kim, M.S. Pergamon Press ; Elsevier Science Ltd 2012 INTERNATIONAL JOURNAL OF HYDROGEN ENERGY - Vol.37 No.10

Hydrogen(H<SUB>2</SUB>)-producing bacterial community structures of the dark fermentation system in a batch reactor were investigated during 48 h by analyzing 16S rRNA gene sequences obtained from pyrosequencing. Organic wastes composed of food waste and sewage sludge were used as a feedstock. After heat treatment (90 <SUP>o</SUP>C for 20 min) of the feedstock, H<SUB>2</SUB> was naturally evolved under anaerobic mesophilic conditions, showing a H<SUB>2</SUB> yield of 2.26 mol H<SUB>2</SUB>/mol hexose<SUB>added</SUB>. The bacterial community structure of the initial inoculum (microbial community at the starting point (0 h)) combined with heat treated food waste and sewage sludge was mainly comprised of Proteobacteria and Bacteroidetes. After 6 h operation, the sequences that belong to other groups except Firmicutes decreased dramatically and were not observed at all in the latter samples. Clostridium spp., which were negligible in the inoculum, took over the main bacterial community by taking charge of H<SUB>2</SUB> production. Among the phylum Firmicutes, the sequences closely related with Clostridium sordellii ATCC 9714<SUP>T</SUP>, Clostridium perfringens ATCC 13124<SUP>T</SUP>, and Clostridium butyricum ATCC 19398<SUP>T</SUP> became predominant in the time series within 48 h. Overall, the results showed how fast the Clostridium spp. overwhelmed the bacterial community in dark fermentative H<SUB>2</SUB> production conditions, where they were at a negligible amount at the start.

조사료원과 급여수준이 한우의 비육능력 (肥育能力) 및 도체성적에 (屠體成績) 미치는 영향

정태영(T . Y . Chung),김종민(C . M . Kim),이왕열(W . Y . Lee),선우훈희(H . H . Sunwoo) 한국영양사료학회 1994 韓國營養飼料學會誌 Vol.18 No.1

Prediction of pathologic staging with magnetic resonance imaging after preoperative chemoradiotherapy in rectal cancer: Pooled analysis of KROG 10-01 and 11-02

Lee, J.H.,Jang, H.S.,Kim, J.G.,Lee, M.A.,Kim, D.Y.,Kim, T.H.,Oh, J.H.,Park, S.C.,Kim, S.Y.,Baek, J.Y.,Park, H.C.,Kim, H.C.,Nam, T.K.,Chie, E.K.,Jung, J.H.,Oh, S.T. Elsevier Science Publishers 2014 Radiotherapy and oncology Vol.113 No.1

Background and purpose: The reported overall accuracy of MRI in predicting the pathologic stage of nonirradiated rectal cancer is high. However, the role of MRI in restaging rectal tumors after neoadjuvant CRT is contentious. Thus, we evaluate the accuracy of restaging magnetic resonance imaging (MRI) for rectal cancer patients who receive preoperative chemoradiotherapy (CRT). Methods and materials: We analyzed 150 patients with locally advanced rectal cancer (T3-4N0-2) who had received preoperative CRT. Pre-CRT MRI was performed for local tumor and nodal staging. All patients underwent restaging MRI followed by total mesorectal excision after the end of radiotherapy. The primary endpoint of the present study was to estimate the accuracy of post-CRT MRI as compared with pathologic staging. Results: Pathologic T classification matched the post-CRT MRI findings in 97 (64.7%) of 150 patients. 36 (24.0%) of 150 patients were overstaged in T classification, and the concordance degree was moderate (k=0.33, p<0.01). Pathologic N classification matched the post-CRI MRI findings in 85 (56.6%) of 150 patients. 54 (36.0%) of 150 patients were overstaged in N classification. 26 patients achieved downstaging (ycT0-2N0) on restaging MRI after CRT. 23 (88.5%) of 26 patients who had been downstaged on MRI after CRT were confirmed on the pathological staging, and the concordance degree was good (k=0.72, p<0.01). Conclusions: Restaging MRI has low accuracy for the prediction of the pathologic T and N classifications in rectal cancer patients who received preoperative CRT. The diagnostic accuracy of restaging MRI is relatively high in rectal cancer patients who achieved clinical downstaging after CRT.

볏짚 요소 - 암모니아 처리시 Urease 원으로 Bacillus pasteurii 배양액의 이용 (2) B. PASTEURII 배양여액과 투석액의 이용이 볏짚의 화학적 조성분과 IN VITRO 소화율에 미치는 영향

배동호(D . H . Bae),안영태(Y . T . Ahn),김상달(S . D . Kim) 한국영양사료학회 1994 韓國營養飼料學會誌 Vol.18 No.3

국내 유통중인 기계발골계육의 품질특성 및 저장특성 비교

이주호(J. H. Lee),최정석(J. S. Choi),김중헌(J. H. Kim),엄태영(T. Y. Eum),김윤희(Y.H. Kim),정준영(J. Y. Jeong),최양일(Y. I. Choi) 충북대학교 동물생명과학연구소 2012 동물생명과학연구 Vol.4 No.-

This study was undertaken to compare the quality characteristics and storage characteristics between mechanically deboned chicken meat (MDCM) in 5 different companies(T1-T5) in Korean. In the chemical composition, T2 showed higher(p<0.05) moisture, T4 showed higher protein(p<0.05), T3 showed higher fat(p<0.05) and T5 showed higher ash(p<0.05). In the pH value, T5 showed lower(p<0.05) pH value than the other raw meat. In the TBA value, T3 showed lower(p<0.05) value than other products from 0 day to 7 day of storage. In the total microbial count, T3 showed lower(p<0.05) value compared to other raw meat from 0 day. But T1 showed lower(p<0.05) from 7 day. In the VBN value, T5 showed higher(p<0.05) from 0 day. And all raw meats showed higher than standard value from 7 day. As a result, MDCM showed difference the quality and storage characteristics the between companies in Korea. It is impossible to store MDCM more than 7 days when stored at 4℃ refrigerator.

Peroxiredoxin II promotes hepatic tumorigenesis through cooperation with Ras/Forkhead box M1 signaling pathway

Park, Y-H,Kim, S-U,Kwon, T-H,Kim, J-M,Song, I-S,Shin, H-J,Lee, B-K,Bang, D-H,Lee, S-J,Lee, D-S,Chang, K-T,Kim, B-Y,Yu, D-Y Macmillan Publishers Limited 2016 Oncogene Vol.35 No.27

<P>The current study was carried out to define the involvement of Peroxiredoxin (Prx) II in progression of hepatocellular carcinoma (HCC) and the underlying molecular mechanism(s). Expression and function of Prx II in HCC was determined using H-ras(G12V)-transformed HCC cells (H-ras(G12V)-HCC cells) and the tumor livers from H-ras(G12V)-transgenic (Tg) mice and HCC patients. Prx II was upregulated in H-ras(G12V)-HCC cells and H-ras(G12V)-Tg mouse tumor livers, the expression pattern of which highly similar to that of forkhead Box M1 (FoxM1). Moreover, either knockdown of FoxM1 or site-directed mutagenesis of FoxM1-binding site of Prx II promoter significantly reduced Prx II levels in H-ras(G12V)-HCC cells, indicating FoxM1 as a direct transcription factor of Prx II in HCC. Interestingly, the null mutation of Prx II markedly decreased the number and size of tumors in H-ras(G12V)-Tg livers. Consistent with this, knockdown of Prx II in H-ras(G12V)-HCC cells reduced the expression of cyclin D1, cell proliferation, anchorage-independent growth and tumor formation in athymic nude mice, whereas overexpression of Prx II increased or aggravated the tumor phenotypes. Importantly, the expression of Prx II was correlated with that of FoxM1 in HCC patients. The activation of extracellular signal-related kinase (ERK) pathway and the expression of FoxM1 and cyclin D1 were highly dependent on Prx II in H-ras(G12V)-HCC cells and H-ras(G12V)-Tg livers. Prx II is FoxM1-dependently- expressed antioxidant in HCC and function as an enhancer of Ras(G12V) oncogenic potential in hepatic tumorigenesis through activation of ERK/FoxM1/cyclin D1 cascade.</P>

농촌형 녹색마을에 신재생에너지 보급을 위한 시설재배 및 농업기계의 CO2 배출량 분석

김종구(J.G. Kim),유영선(Y.S. Ryou),강연구(Y.K. Kang),김영화(Y.H. Kim),장재경(J.K. Jang),김현태(H.T. Kim),서광욱(K.W. Seo),이승기(S.K. Lee),조희제(H.J. Cho),강지원(J.W. Kang) 유기성자원학회 2011 유기물자원화 Vol.19 No.1

화석에너지 의존도를 줄이면서 CO2 배출량을 낮추기 위하여 정부에서는 녹색마을을 선정하고 에너지자급률을 40% 수준으로 높이려는 계획을 추진 중이다. 본 연구는 각 농업 분야 중에서 농기계의 사용과 재배 시설에 있어서의 에너지 사용량을 파악하고 이를 바이오디젤로 대체하였을 때의 CO2 저감수준을 분석하고자 하였다. 이를 위하여, 농업 각 분야별 에너지 소비수준의 분석, 그리고 실천 가능한 신재생 에너지원의 선정이 요구된다. 경종재배의 전체 연간온실가스 배출량은 5,667,258 t-CO2이고, 그 중 시설 부문은 4,932,607 t-CO2인 것으로 분석되었으며, 농업시설 부문 중 에너지원별로 보면 경유가 3,105,707t-CO2, 중유가 1,370,578 t-CO2를 배출하는 것으로 분석되었다. 우리나라 시설작물의 단위 면적당 온실가스 평균배출량은 29,418 t-CO2/ha인 것으로 나타났다. 농기계별 2007년 총에너지소비량을 살펴보면 트랙터가 284,763 kL로 가장 높게 나타났으며, 동력 경운기221,314 kL, 곡물건조기 145,524 kL, 콤바인 72,537 kL 등의 순이었다. 전라북도 G시를 대상으로 이용 중인 시설재배와 농업기계의 이산화탄소 배출량을 비교분석한 결과,바이오디젤로 전환하면 약 7% 정도의 CO2 감소효과가 있는 것으로 분석되었다. In order to reduce dependence on the fossil fuels and CO2 gas emission in farming activities, the government has pushed ahead with making the self-sufficiency of farming energy up 40% level in green villages. The objectives of this study are to survey the energy consumption of horticultural facilities or agricultural machineries, and to analyze the reduced CO2 gas emission level from fossil fuel to bio-diesel fuel. For the implement of this study, it is necessary to analyze the energy consumption level in the various sector of farming activities, and available renewable energy sources should be selected. Annual total CO2 gas emission in the tillage farming sector was analyzed as 5,667,258 t-CO2 and that in the horticultural facilities occupied 4,932,607 t-CO2, while the CO2 gas emission level of diesel fuel was 3,105,707 t-CO2, and that of the heavy oil showed 1,370,578 t-CO2. The average CO2 gas emission level of horticultural facilities in the country was analyzed as 29,418 t-CO2/ha. Among the total energy consumption of agricultural machineries, tractor used 284,763kL, power tiller spent 221,314 kL, grain drier consumed 145,524kL and combine tractor expend 72,537kL. From the comparison of CO2 gas emission level between fossil fuel and bio-diesel fuel for the horticultural facilities or agricultural machinery in G-City, Jeonbuk Province, the CO2 gas emission level can be reduced by 7% through replacing the fuel from fossil to biodiesel.