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부갑상선 호르몬이 조골세포군의 교원분해효소 유리에 미치는 영향에 관한 연구
이인석,김관식,정동균 대한구강생물학회 1990 International Journal of Oral Biology Vol.14 No.1
Bone resorption is accomplished by the removal of both mineral and the organic matrix. The cellular origin of bone collagenase that is responsible for the removal of unmineralized organic matrix has remained equivocal. This study was performed to investigate the effect of PTH on the release of collagenase and cellular origin of bone collagenase in bone cell populations. Calvaria from fetal rat at 19-day of gestation, were sequentially digested by the enzyme solution consisted of collagenase, trypsin and EDTA for 10 min (PopulationI), 10 min (II), 10 min (III), 20 min (IV) and 20 min (V). Each bone cell population was primarily cultured for 6-7 days and splited to 5.0×10^5 cells/35mm culture dish and then incubated for 6 days with MEM with or without PTH (1.0U/ml). Collected medium was incubated with type I collagen for 6h at 37℃ and then used for hydroxyproline determination by Woessner's method. The results were as follows. 1. There were no significant differences of collagenase activity among 5 bone cell populations. 2. PTH (1.0U/ml) had no effect on the collagenase activity in the population I, II, III and V. 3. PTH (1.0U/ml) significantly increased collagenase activity in population IV.
김관수,최재석,이순영,장순용 慶尙大學校 工科大學 自動化및컴퓨터應用技術硏究所 1999 自動化 및 컴퓨터應用技術 Vol.6 No.1
In this paper, the direct and indirect neural adaptive controller are combined based on the Lyapunov synthesis approach. The proposed adaptive controller is constructed from RBF neural network and a set of fuzzy IF-THEN rules. And the weighting parameters are adjusted on-line according to some adaptation law for the purpose of controlling the plant to track a given trajectory. In this scheme, fuzzy IF-THEN rules are used to decide the combined weighting factor. It is shown that all the signals in the closed-loop system are uniformly bounded under mild assumptions. The effectiveness of the proposed control scheme is demonstrated through the control of one-link rigid robotics manipulator.
임석균,이기혁,김영균,김수관,이병준,여환호,서재훈 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.3
Bilateral cleft lip reconstruction can be performed in one-or two-stages. The choice depends on the surgeon's proficiency and experience. However the type and severity of the cleft must be considered. Complete or incomplete symmetric, bilateral clefts in which the premaxilla is within the alveolar arch or slightly protruded can be successfully treated with simultaneous lip reconstruction on both sides. There are some advantages that one-stage lip reconstruction facilitates creation of a symmetric and balanced lip, retraction of protruding premaxilla and the parent's psychologic satisfaction. However, there are some disadvantages such as micrognathia of the upper jaw by severe scar formation and poor adaptation of flap margins by severe tension. The surgeon must be able to judge and exploit the advantages offered by one-stage approach. We present the case report of one-stage reconstruction of primary bilateral cleft lip with literature reviews.
Gwan-Seok Lee,Seol-Mae Lee,Chang-Seok Kim,Hong-Soo Choi,Kyeong-Yeoll Lee,Wonhoon Lee 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10
Field surveys for the B. tabaci species complex were conducted from 2009 to 2013 in Korea, and the results were compared with published data of the B. tabaci complex. Three species, Mediterranean, Middle East-Asia Minor 1, and JpL, were determined from several regions based on mitochondrial COI sequences. The Mediterranean was mainly collected in greenhouses, displacing the earlier invasive species, Middle East-Asia Minor, and the JpL was collected in the field. The JpL is newly confirmed as a unique species of B. tabaci complex in Korea and Japan.
Gwan-Seok Lee,Seol-Mae Lee,Hae-Ryun Kwak,Chang-Seok Kim,Hong-Soo Choi,Sukchan Lee,Kyeong-Yeoll Lee 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.10
The sweet potato whitefly, Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae), is one of the most important agricultural pests by direct feeding and begomovirus transmission in the world. B. tabaci species complex consisting of about 30 species worldwidely has been mainly distinguished by molecular methods because of difficulties detecting morphological differences. In Korea, distribution of two exotic groups, the Mediterranean (Q biotype) and the Middle East-Asia Minor 1 (B biotype), were confirmed by comprehensive molecular methods in 2012. Of them, the Q biotype as a vector of Tomato yellow leaf curl virus (TYLCV) has caused significant crop yield losses. Tobacco leaf curl virus (TbLCV), one of four begomoviruses in Korea, was first reported in 2011. It damaged, although not frequent, to greenhouse tomatoes at Iksan, Gimje, Sunchang, and Jeju. The vector transmitting TbLCV, however, is not known yet. In this study, we sampled an unknown B. tabaci populations on leaves of the Japanese honeysuckle (Lonicera japonica) at Iksan in outdoor conditions. PCR analysis revealed that some populations were infected with TbLCV. We identified an unknown indigenous genetic group named as JpL and clarified its phylogenetic relationships based on nucleotide sequences of mitochondrial cytochrome oxidase I gene.
Wonhoon Lee,Seunghwan Lee,Gwan-Seok Lee 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04
Bemisia tabaci (Hemiptera: Aleyrodidae) is one of the most important insect pests in the world. In the present study, the taxonomic status of B. tabaci and the number of species composing the B. tabaci complex were determined based on 1,059 COI sequences of B. tabaci and 509 COI sequences of 153 hemipteran species. The genetic divergence within B. tabaci was conspicuously higher (on average, 11.1%) than interspecific genetic divergence within the respective genera of the 153 species (on average, 6.5%). This result indicates that B. tabaci is composed of multiple species that may belong to different genera or subfamilies. A phylogenetic tree constructed based on 212 COI sequences without duplications revealed that the B. tabaci complex is composed of a total of 31 putative species, including a new species, JpL. However, genetic divergence within six species (Asia II 1, Asia II 7, Australia, Mediterranean, New World, and Sub Saharan Africa 1) was higher than 3.5%, which has been used as a threshold of species boundaries within the B. tabaci complex. These results suggest that it is necessary to increase the threshold for species boundaries up to 4% to distinguish the constituent species in the B. tabaci complex.
Seol Mae Lee,Gwan-Seok Lee,Jong Wook Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05
The subfamily Acaenitinae Foerster, 1869 is a small-sized group belonging to the family Ichneumonidae. It includes 250 species in 26 genera from Ethiopian, Oriental, and Palaearctic region. However, only 14 species of six genera, Arotes, Coleocentrus, Jezarotes, Phaenolobus, Spilopteron and Yamatarotes have been reported from Korea. This subfamily can be easily recognized from other ichneumonids by the combination of the following characters: subgenital plate very elongate; tarsal claws with accessory tooth. Members of Acaenitinae are parasitoids of wood-boring Coleoptera, Hymenoptera and Lepidoptera. Among them, some of parasitoids of Cerambycidae are known as intermediate carrier of Bursaphelenchus xylophilus (Nematoda: Parasitaphelenchidae). In this study of the Korean Acaenitinae, we found the genus Yezoceryx from Korea for the first time, including newly recorded two species, Y. sp.1 and Y. sp.2. We provide description of the genus and two species with a key to the Korean genera of the subfamily Acaenitinae.
Taxonomy of the subfamily Acaenitinae (Ichneumonidae: Hymenoptera) from Korea
Seol Mae Lee,Gwan-Seok Lee,Jong Wook Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05
The subfamily Acaenitinae Föster, 1869 is a small-sized subfamily belonging to the family Ichneumonidae. Members of Acaenitinae distributes in Ethiopian, Oriental, and Palearctic Regions. Twenty six genera have been recorded in the World, and six genera are known from Korea. The biology of the Acaenitinae is not well known, however, some hosts are recorded from Cerambycidae in Coleoptera (Gardiner, 1960), Sesiidae and Tortricidae in Lepidoptera (Ulbricht, 1909; Starke, 1956). Generally female is easily distinguished from that of other subfamily of Ichneumonidae. They can be recognized by their very elongate subgenital plate, and by the auxiliary tooth on the tarsal claws. Male can be also easily recognized by the form of the claws and the facial shape. In this study, we found a newly recorded genus Yezoceryx, belonging to the Tribe Acaenitini, with two species in Korean fauna. Illustrated key of species and genera, diagnosis and photographs of each species are provieded.