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마늘의 유엽(幼葉) 및 양파의 인편배양(鱗片培養)으로부터 기관분화(器官分化)에 관한 연구(硏究)
이은모,이영복,Lee, Eun Mo,Lee, Yeong Bok 충남대학교 농업과학연구소 1982 Korean Journal of Agricultural Science Vol.9 No.2
마늘의 유엽(幼葉)과 양파의 인편(鱗片)을 공시재료(供試材料)로 배양(培養)할 때, 엽영(葉齡) 및 부위(部位)에 따른 기관분화(器官分化)의 차이(差異)와 아울러 기관분화(器官分化)에 영향(影響)을 주는 cytokiain과 auxin, 온도(溫度) 및 광(光)의 영향(影響)을 검토(檢討)하였던 바, 그 결과(結果)를 요약(要約)하면 다음과 같다. 1. 마늘의 유식물체(幼植物體) 분화(分化)는 $BA\;2mg/{\ell}+NAA\;1mg/{\ell}$ 처리구(處理區)에서 가장 좋았으며, 유엽(幼葉)의 외측부위(外側部位) 중(中) 기부(基部)에서 주(主)로 되었다. 2. 유식물체(幼植物體)는 광조건(光條件)보다 암조건(暗條件)에서 배양(培養)한 것이 분화(分化)가 좋았다. 3. 배양(培養) 온도(溫度)에 있어서는 $20^{\circ}C$가 $28^{\circ}C$에 비하여 유식물체(幼植物體) 분화(分化)에 효과적(效果的)이었다. 4. 발근(發根)은 무처리구(無처理區) 및 BA 단독처리(單독처理)에서는 불량하였고, $BA\;2mg/{\ell}+NAA\;1mg/{\ell}$ 처리구(處理區) 중(中) 외부(外部) 유엽(幼葉)의 기부(基部)에서 발생이 양호(良好)하였다. 5. 햇 마늘의 유식물체(幼植物體) 분화(分化) 양상(樣相)은 묵은 마늘의 경우와 비슷하였다. 6. 묵은 양파의 맹아엽(萌芽葉) 배양(培養)에서는 엽영(葉齡)이나 부위(部位)에 따른 유식물체(幼植物體) 분화(分化)의 차이(差異)는 보이지 않았고, 전반적으로 $BA\;2mg/{\ell}+NAA\;1mg/{\ell}$에서 비교적 좋은 결과(結果)를 보였다. 7. 묵은 양파나 햇 양파나 내측(內側) 인편(鱗片) 중(中) NAA와 BA의 복합 처리구(처理區)에서 분화(分化)되었다. 8. callus는 NAA처리구(처理區)에서 주로 분화(分化)되며, callus가 분화(分化)된 후에 발근(發根)이 되었다. Leaflets of Allium sativum L. (garlic) and leaf scales Allium cepa L. (onion) were cultured on the Murashige and Skoog(MS) medium to study the effects of plant growth regulators, temperature and light on the formation of shoot and root. And also, the potentiality of shoot or root formation of tissues in accordance with the leaf age and the region was discussed. In the experiment with garlic, the formation of shoot and root was more effective on the medium containing $BA\;2mg/{\ell}$ and $NAA\;1mg/{\ell}$ and it occured on the basal region of outer leaflets. The shoot formation was more effective at $20^{\circ}C$ than $28^{\circ}C$ under 16-hour daylength, and it was more effective in darkness than light condition at $28^{\circ}C$. The results of shoot and root formation on the new cloves of garlic harvested in 1982 were similar to those the old cloves of garlic harvested in 1981. In the experiment with leaf seales of onion, when the tissues of dormant leaf scales of onions harvested in 1981 were explanted on the MS medium, a few shoots were formed on the medium containing $BA\;2mg/{\ell}$ and $NAA\;1mg/{\ell}$. In this case,there were no differences on the shoot formation among the age and region of leaflets. On the culture of leaf scales, the shoot was not formed on the leaf scales located at outer or middle region, but it was formed on the medium containing BA accompanied with NAA at inner scales. On the medium complemented with BA alone, the tissues were not differentiated, but on the medium complemented with NAA, the callus was formed from tissues and then the root was formed through the callus.
초저온 냉동 시스템에서 2원냉동 팽창밸브의 압력 변화 특성에 관한 연구
이은모(Eun Mo Lee):박경주(Kyeong Joo Park) 산업기술교육훈련학회 2014 산업기술연구논문지 (JITR) Vol.19 No.3
The different evaporation temperatures and condensation temperatures, using a refrigerant with a characteristic for high temperature side and low temperature side Cycle to Cycle, each configured for a one side to the other Cycle in binary refrigeration to get the evaporation temperature of the low temperature impact test. Results could be confirmed that the other side Cycle 1 of the evaporator and the condenser from the outlet side of the heat exchanger by heat exchange with each other Cascade Cycle of one impact. In other words, although not a random operation Cycle primary components of the increase in the amount of open secondary expansion device to transfer more of the heat exchanger into the primary through the Cascade and this change increases 1 Conpressor suction side refrigerant status to ultimately 2 was confirmed by testing the arrival of a difference in the primary winding Cycle as well. Was confirmed that the evaporation temperature of the low temperature can be obtained affect each other through the Cascade Cycle device that is configured independently of the refrigerator through the two won in this experiment, respectively.
빙핵활성단백질의 N-terminal부분을 이용한 녹색형광단백질의 Zymomonasmobilis세포 표면 발현
이은모(Lee,Eun-Mo ),최신건(Choi,Shin-Geon) 강원대학교 산업기술연구소 2009 産業技術硏究 Vol.28 No.B
Green fluorescent protein (GFPuv) was displayed on the surface of ethanol-producing bacteria Zymomonas mobilis using N-terminaldomain of ice nucleation protein (INP) as an anchoring motif. To evaluate the ice nucleation protein as plausible anchor motif in Z. mobilis, GFPuv gene was subcloned into Zymomonas expression vector yielding pBBR1MCS-3/pPDC/INPN/GFPuv plasmid., INP-GFPuv fusion protein was expressed in Z.mobilis and its fluorescence was verified by confocal microscopy. The successful display of GFPuv on Zymomonas mobili ssuggest that INP anchor motif could be used for future fusion partner in Z. mobili sstrain improvement.
방울토마토 수경재배 시 착과 절위 증가에 따른 공급액, 배액 및 식물체의 무기성분 농도 변화
이은모(Eun Mo Lee),박상규(Sang Kyu Park),김경제(Gyoung Je Kim),이봉춘(Bong Chun Lee),이희철(Hee Chul Lee),윤여옥(Yeo Uk Yun),박수복(Soo Bok Park),최종명(Jong Myoung Choi) (사)한국생물환경조절학회 2017 생물환경조절학회지 Vol.26 No.4
수경재배 시스템에 있어서 배액의 재활용은 생산비 절감 및 환경오염 방지를 위하여 중요하다. 방울토마토를 반촉성 수경재배 하면서 생육 단계별 공급액, 배액 및 잎의 무기성분 분석을 통하여 순환식 수경재배 시스템 개발을 위한 기초자료를 확보하고자 본 연구를 수행하였다. 연구목적을 달성하기 위해 주기적으로 공급액, 배액 및 식물체 잎을 채취한 후 무기물 함량을 분석하였다. 생육 초기에는 배액의 EC가 공급액과 비슷한 약 2.0dS·m<SUP>-1</SUP>였지만, 생육 후기로 갈수록 높아져 9화방 착과기에는 4.5dS·m<SUP>-1</SUP>였다. 영양생장이 왕성한 생육 초기의 pH는 6.4~6.7 범위였으나 생식생장이 강해진 생육 후기에는 5.9~6.1로 낮아지는 경향이었다. 생육 초기에는 공급액과 배액의 NO₃-N, P, K, Ca 및 Mg 농도가 비슷하였지만 생육 후기로 갈수록 공급액보다 배액의 농도가 높아지는 경향이었다. 생육 초기에 잎의 T-N 함량이 높았지만 후기로 갈수록 낮아지는 경향이었다. K와 Ca 함량은 생육 초기에는 낮았으나 후기로 갈수록 높았으며, P와 Mg 함량은 생육초기부터 후기까지 유사한 수준으로 분석되었다. 이상의 결과는 토마토 수경재배를 배액 재활용에 있어 무기원소 농도 변화를 교정하기 위한 기초자료로 활용될 수 있을 것이다. Production cost as well as environmental contamination can be reduced by reuse of drained nutrient solution in hydroponic. This research was conducted to obtain the information in changes in inorganic elements concentration of supplied and drained nutrient solution as well as of plant leaves. To achieve the objective, the samples of supplied and drained solution and cherry tomato leaf tissues were periodically collected and analyzed during the hydroponic cultivation. The electrical conductivity (EC) of supplied and drained nutrient solution in early growth stage of cherry tomato were measured as around 2.0 dS·m<SUP>-1</SUP>, but those values move up with the passage of time reaching to 2.0 dS·m<SUP>-1</SUP> at flowering stage of 9th fruiting node. The pHs of drained solution in early growth stage were 6.4 to 6.7, however those showed a tendency to get lowered to 5.9 to 6.1 as time passed during the crop cultivation. The concentration differences of NO₃-N, P, K, Ca, and Mg between supplied and drained solution were not distinctive until flowering stages of 4th fruiting nodes, while those in drained solution moved up after the stage. The tissue N contents of leaves decrease gradually and those of K and Ca increased as crops grew. However, Tissue P and Mg contents were maintained similarly from transplant to end-crop. The above results would be used in correction of drained nutrient solution when element compositions are varied compared to supplied solution in hydroponic cultivation of tomatoes.