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崔鎭浩,林采喚,崔映準,卞大錫,金昌睦,吳成基 경희대학교 부설 식량자원개발연구소 1986 硏究論文集 Vol.7 No.-
우리나라 전역에 걸쳐서 分布하고 있는 뱀장어의 營養學的 基礎資料를 提示하기 위하여 天然産과 養殖産으로 分類하여 蛋白質의 組成, 蛋白質의 아미노산酸 組成을 比較, 分析하였다. 그리고 天然 및 養殖産 뱀장어 肉蛋白質의 組成을 엄밀히 檢討하기 위해 肉蛋白質中 比較的 많은 量을 차지하는 筋形質蛋白質과 筋原纖維蛋白質을 SDS-PAG電氣泳動 分析하여 subunit의 差를 比較하였다. 또한 사료蛋白質의 아미노酸 組成을 分析함으로서 肉蛋白質의 아미노酸 組成에 미치는 영향도 아울러 比較, 檢討하였다. 天然 및 養殖産 뱀장어의 粗蛋白質 合量은 各各 14.3%와 15.4%이었으며, 粗脂肪은 25.0%와 29.7%로서 養殖産 뱀장어의 脂肪 含量이 天然産에 比하여 높은 편이있다. 肉蛋白質을 構成하는 蛋白質 組成은 天然産의 경우 筋形質蛋白質이 30.78%, 筋原纖維蛋白質이 59.02%, 細胞內殘渣蛋白質이 9.73%, 基質蛋白質이 2.47%이었으며, 養殖産은 筋形質蛋白質이 31.81%, 筋原纖維蛋白質이 58.37%, 細胞內殘渣蛋白質이 8.16%, 基質蛋白質이 1.80%를 차지하여 天然産과 養殖産뱀장어의 肉蛋白質 組成은 비교적 유사함을 알 수 있었다. 筋形質蛋白質과 筋原纖維蛋白質 分劃의 一部에 대하여 SDS-PAG電氣泳動 分析을 실시한 結果, 天然産 뱀장어의 筋形質蛋白質은 16개의 subunit로 構成되어 있었고, 養殖産은 分子量이 36,500, 46,000, 58,500, 75,000, 170,000, 235,000에 해당하는 4개의 subunit가 더 많은 22개의 subunit로 構成되어 있었다. 한편 筋原纖維蛋白質은 天然産이 14개의 subunit이었으며, 養殖産은 分子量 45,000에 해당하는 未知의 subunit가 하나 더 많은 15개로 이루어져 있었다. 肉蛋白質의 蛾眉노산 組成은 天然産이 養殖産에 比하여 glycine含量이 다소 높은 것을 제외하고는 대체로 비슷하였으며, lysine, aspartic acid, glutamic acid, glycine이 전체아미노酸의 45% 가량을 점하고 있었다. 사료蛋白質의 아미노酸 組成은 뱀장어의 필수아미노산 要求量을 만족시킬 만큼 양호한 組成을 보여주었으며, aspartic acid와 glutamic acid를 제외하고는 魚肉의 축적단백질로서 魚肉蛋白質의 아미노 組成과 비례관계를 갖는 것으로 판단되었다. The muscles of wild and cultured eel, Anguilla japonica, were analyzed for the protein composition and amino acid profile. The differences of the subunit distribution for the sarcoplasmic and myofibrillar proteins were discussed with sodium dodecylsulfate(SDS) polyacryamide gel electrophoresis. The muscle protein in wild eel was composed 30.78% of sarcoplasmic, 59.02% of myofibrillar, 9.73% of residual intracellular and 2.47% of stroma fraction. That in cultured eel was composed of 31. 81%, 58.37%, 8.16% and 1.80%, respectively. The sarcoplasmic and myofibrillar proteins were composed of 16 and 14 subunits in wild eel, and 22 and 15 subunts in cultured eel. The sarcoplasmic protein between wild and cultured muscles showed a similar trend in the subunits, except a few subunits such as 36,500, 46,000, 58,500, 75,000, 170,000 and 235,000 daltons in cultured eel. Only the existence of 45,000 dalton subunit was the difference between wild and cultured eel in myofibrillar protein. The distribution patterns of total amino acid in muscles of wild and cultured eel were found to be very similar trend, although glycine content in wild eel was slightly higher than that in cultured one.
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대구한의대 비만클리닉 내원 환자 62명에 대한 임상적 관찰
허진일,김동우,박경,김대준,변준석,최빈혜,Hur, Jin-Il,Kim, Dong-Woo,Park, Kyung,Kim, Dae-Jun,Byun, Joon-Seok,Choi, Bin-Hye 대한한방내과학회 2006 大韓韓方內科學會誌 Vol.27 No.2
Objectives : The purposes of this study are to evaluate the effect of treatment in obesity clinic in Dae-Gu Hanny University and to improve the oriental medical methods of treatment in obesity patients. Methods : 62 patients were classified into four groups by Body Mass Index(BMI) : normal, overweight, 1st obesity and 2nd obesity groups. Weight, BMI, body fat rate and abdominal fat rate were compared in each group by t-test. Results : In distribution of sex, male were 10(16.1%), female were 52(83.9%). Mean age was $31.0{\pm}2.59$ and the patients in twenties and thirties were the most. Mean weight was $71.80{\pm}4.08kg$ and 1st obesity group(23) was the most. Mean BMI was $27.11{\pm}1.20(kg/m^2)$. BMI Changes in obesity groups treated by obesity clinic were $0.95{\pm}0.68kg/m^2$ in normal group, $1.45{\pm}0.38kg/m^2$ in overweight group, $2.67{\pm}0.48kg/m^2$ in 1st obesity group and $4.01{\pm}1.10kg/m^2$ in 2nd obesity group. The changes in all groups were significant. The more the obesity degree was. the more weight loss was. Conclusions : The weight and BMI in patients were almost decreased significantly when they treated. It resulted that the effect of oriental medical treatment in obesity was positive. This study is the effect of treatment in obesity by several medical methods and it is thought that the study of the effects of treatment in obesity by each medical methods that were used in this study is necessary.
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소적정원산(消積正元算)으로 호전된 적증(積證)환자 1례 보고
허진일,조동희,김동우,임희용,오중한,김대준,변준석,최빈혜,Hur, Jin-Il,Cho, Dong-Hee,Kim, Dong-Woo,Lim, Hee-Yong,Oh, Jung-Han,Kim, Dae-Jun,Byun, Joon-Seok,Choi, Bin-Hye 대한한방내과학회 2005 大韓韓方內科學會誌 Vol.26 No.2
This study was designed to evaluate the effects of the oriental medicine(Sojeockjeongwonsan) on a Jeock-Jeung patient. The clinical data was analyzed on a patient with Jeock-Jeung, deemed due to Gangiulgyul(肝氣鬱結) and Junggiheoyak(正氣虛弱). The patient’s main symptoms were abdominal pain and abdominal mass. The patient was admitted to Dae-Gu Hanny University Dae-Gu Oriental Medicine Hospital. Clinical symptoms, including abdominal pain, abdominal mass and general condition, improved after treatment. This study suggests that Sojeockjeongwonsan is significantly effective in treatment of a Jeock-Jeung.
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Byun, Dae-Seok,Chang, Young-Chae,Kang, Myung-Hwa Korean Society of Life Science 2001 Journal of Life Science Vol.11 No.1
Subtilisin YaB, produced by alkalophilic Bacillus strain YaB, is an extracellular alkaline serine protease having 55% homology to subtilisin BPN'. It is synthesized as a 378-amino acid preproenzyme and secreted into the culture medium as a 265-amino acid mature protease. To examine the role of pro-sequence for the secretion of subtilisin YaB, we have studied the expression, in Bacillus subtilis, of a mutant preprosubtilisin YaB in which active site Ser214 is substituted with Cys. The use of a six protease-deficient strain, WB600, was required for its efficient production. The prosubtilisin YaB, thus produced, was indeed secreted into the culture medium and was processed to its mature form upon treatment with exogenously added active subtilisin YaB. From these results, we have concluded that the processing of pro-sequence is not essential for the secretion of the enzyme.
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Synthesis and Characterization of Bandgap-modulated Organic Lead Halide Single Crystals
Dae Young Park,Hye Ryung Byun,A Young Lee,Ho Min Choi,Seong Chu Lim,Mun Seok Jeong 한국물리학회 2018 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.73 No.11
Single crystal of organic lead halide (CH3NH3PbX3; CH3NH+ 3 = methylammonium (MA) , X = Cl; Br; I) is the best candidate for material intrinsic property studies due to no grain boundary and high crystal quality than the film having a lot of grain boundary and surface defects. The representative crystallization methods are inverse temperature crystallization (ITC) and anti-solvent vapor assisted crystallization (AVC). Herein, we report bandgap modulated organic lead halide single crystals having a bandgap ranging from 2:1 eV to 3 eV with ITC and AVC methods. The bandgap modulation was achieved by controlling the solvents and chloride-to-bromide ratio. Structural, optical and compositional properties of prepared crystals were characterized. The results show that the crystals synthesized by the two crystallization methods have similar properties, but the halide ratios in the crystals synthesized by the AVC method are controlled more quantitatively than the crystals synthesized by ITC.
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Dae Young Park,Hye Ryung Byun,Hyojung Kim,Bo Ra Kim,Mun Seok Jeong 한국물리학회 2018 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.73 No.11
The power conversion eciency of perovskite solar cells has reached 23.3%. Although significant developments have been made through intensive studies, the stability issue is still challenging. Passivation of perovskite solar cells with a transparent polymer provides better stability; however, there are a few disadvantages of organic polymer such as low thermal stability, weak adhesion and the lack of water retention ability. In this work, we prepared a dual Parylene-F/C layer with 3- methacryloxypropyltrimethoxysilane, A-174, to combine the advantages of organic and inorganic materials. As a result, A-174 treated dual Parylene-F/C layer demonstrated improved passivation effects compared to a single Parylene layer due to the strong binding of Parylene and the water retention ability by SiO2 formed from A-174. This synergetic effects can be expanded to the combination of other organic materials and organosilane compounds.
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