Inhibition of protein kinase CKII activity by euchrestaflavanone B purified from Cudrania tricuspidata.

Kim, Soon-Hee,Yoon, Soo-Hyun,Lee, Byong Won,Park, Ki Hun,Kim, Young Ho,Bae, Young-Seuk Pergamon Press 2005 Oncology Research Vol.15 No.6

<P>The CKII (EC 2.7.1.37) inhibitory compound was purified from the root barks of Cudrania tricuspidata and identified as (2S)-2-[2,4-dihydroxy-5-(3-methyl-but-2-enyl)-phenyl]-5,7-dihyroxy-6-(3-methyl-but-2-enyl)chroman-4-one (euchrestaflavanone B). Euchrestaflavanone B was shown to inhibit the phosphotransferase activity of CKII with IC50 of about 78 microM. Steady-state studies revealed that euchrestaflavanone B acted as a competitive inhibitor with respect to the substrate ATP. A value of 16.4 microM was obtained for the apparent Ki. Concentration of 0.8 microM euchrestaflavanone B caused 50% growth inhibition of human cancer cells U937 and HeLa. Euchrestaflavanone B-induced cell death was characterized with the cleavage of poly(ADP-ribose) polymerase and procaspase-3, indicating that the inhibitor triggered apoptosis. Because protein kinase CKII is involved in cell proliferation and oncogenesis, these results suggest that euchrestaflavanone B may function by inhibiting oncogenic disease, at least in part, through the inhibition of CKII activity.</P>

學敎運營의 實態와 改善方案에 관한 硏究

金鍾喆,李鍾淑,陳東燮,許柄其 덕성여자대학교 고등교육연구소 1990 敎育硏究 Vol.2 No.-

마이크론 크기의 Poly(Ethyl Methacrylate) 분산 미립자 형성에 관한 연구

김병철,손태원,이기창 慶尙大學校生産技術硏究所 1991 生産技術硏究所論文集 Vol.7 No.-

Considerable interest has been recently generated by the preparation of large monodisperse polymer particles by dispersion polymerization. In a dispersion polymerization process, the reaction mixture starts out as a homogeneous solution and the resulting polymer precipitates as spherical particles. The present study focused on dispersion polymerization of Ethly Methacrylate in water-ethanol mixture with a Azo initiator and a polymer stabilizer, poly(vinyl pyrrolidone) with an anionic costabilizer, Aerosol MA. Effects of polymerization parameters, such as monomer concentration, initiator concentration, stabilizer concentration, different molecular weight of stabilizer, comonomer concentration, and polymerization temperature on average particle size and size distribution have been investigated. The polymerization recipe of preparation of uniform PEMA particles in 2-6 ㎛ size range has been also made.

퍼지 PID 제어기를 利用한 AGV의 走行制御에 關한 硏究

김병기,김윤상,안비오,이종성,안두수 成均館大學校 科學技術硏究所 1999 論文集 Vol.50 No.1

The AGV(Automatic Guided Vehicle) becomes one of the most important parts in the industrial factory. Using fuzzy PID controller, we have simulated the AGV system following the guide path according to the varying velocity and experimented AGV's driving controller with microprocessor(Intel 80C196KC). In the experimental results, we compared the PID controller with the fuzzy PID controller and we can see that the performance of the fuzzy PID controller is better than that of the PID controller in the aspect of guide path tracking.

W치환 2205 이상 스테인리스강의 크리프 특성 평가에 관한 연구

김기엽,최병호,남기우,안용식 한국비파괴검사학회 2004 한국비파괴검사학회지 Vol.24 No.1

22Cr-5Ni 2상 스테인리스강의 합금원소 중 몰리브덴(Mo) 원소를 텅스텐(W)으로 치환하여 크리프거동에 미치는 영향을 조사하였다. 크리프시험은 600℃와 650℃의 온도에서 실시하였다. 650℃ 크리프 시에는 취약한 금속간화합물인 σ상이 석출하였으며 이것은 크리프수명을 급격히 감소시키는 역할을 하였다. Mo에 대한 W원소의 치환은 일반적으로 σ상의 석출을 지연시킨다고 알려져 있으나, 2wt.%까지 W 치환을 실시한 본 연구범위에서는 크리프 수명 및 최소 크리프속도에 뚜렷한 영향을 미치지 못하였다. 크리프손상의 정도를 평가하기 위하여 크리프시편에 대한 초음파검사를 실시하였다. 크리프온도 600℃에서는 크리프시편의 수명이 증가함에 따라 음속이 비례적으로 증가하였으나 650℃에서는 반대로 감소하였다. The effect of the substitution of Tungsten(W) for Molybdenum(Mo) on the creep behaviour of 22Cr-5Ni duplex stainless steel(DSS) has been investigated. Creep tests were carried out at 600℃ and 650℃. Intermetallic σ phase is precipitated during creep at 650℃, at which creep rupture time was much lower compared with at 600℃. The substitution of W for MO in the duplex stainless steel was known to retard the formation of σ phase. Minimum creep rate and creep rupture time, however, were hardly influenced by the substitution of 2wt.% W. An ultrasonic measurement for the creep specimens has been carried out for the evaluation of creep damage. The sound velocity increases propotionally with the increase of creep rupture time at 600℃ of creep temperature. On the contrary, the sound velocity decreases with the increase of rupture time at 650℃, which can be correlated with the microstructural evolution during creep.

효율적인 공급체인관리를 위한 협력업체 평가구조분석 및 선정에 관한 연구

金鎭鎬,李丙玘 明知大學校 産業技術硏究所 2004 産業技術硏究所論文集 Vol.23 No.-

In supply chains, coordination between a purchaser and supplier is regarded as the most important issue when partnership of organizations is considered. Since the suppliers are external to the purchaser and poor coordination between them results in excessive delays and ultimately leads to poor customer service, purchasers need a new methodology to select suppliers and to manage and enhance the partnerships between purchaser and suppliers. This paper studies a supplier evaluation structure and then suggests a methodology using fuzzy theory for selecting an effective supplier. Several quantitative and qualitative evaluation factors should be analyzed in the supplier evaluation structure.

광투과방식과 연속식 GC분석방식을 이용한 대기 중 BTX 성분의 정량적 분석

김기현,진병복 경희대학교 환경연구소 2002 環境硏究 論文集 Vol.11 No.-

To study the applicability of DOAS system on the analysis of VOC including benzene, toluene, and xylenes in air, we made a comparative measurement using an on-line GC system. Results of our study indicate that the compatibility between two different methodologies is highly distinct among species, when checked in terms of various standards. When the differences between the two are expressed in terms of percent differences, the level of agreement between the two were found on the order of toluene (-2.7 %), xylenes (-45.5 %), and benzene (-80.8 %). Moreover, if the compatibility of the data sets is checked by correlation analysis, their relationships were found as follows: Benzene (DOAS) = 0.485 Benzene (GC) + 0.199 (r=0.236) Toluene (DOAS) = 0.900 Toluene (GC) + 0.280 (r=0.738) Xylenes (DOAS) = 0.812 Xylenes (GC) + 0.114 (r=0.592) According to our comparative analysis, it is found that toluene exhibits the strongest compatibility between the two systems, while benzene tends to record the least agreement. Although the system-to-system bias is different among different chemical species, the overall results of our study suggest that DOAS system can be useful enough to provide valuable information of temporal and spatial distribution patterns of major aromatic VOCs in air.

공급체인관리하에서 퍼지이론을 이용한 효율적인 협력업체 선정에 관한 연구

김진호,이병기 한국경영과학회 2003 한국경영과학회 학술대회논문집 Vol.- No.1(1)

공급체인관리(SCM)에서, 고객의 반응에 민감하게 반응할 수 있기 위해서는 무엇보다도 부품이나 자재를 공급하는 협력업체의 능력이 중요한 요인으로 작용한다. 본 논문은 퍼지이론을 이용하여 과학적이고 효율적으로 협력업체를 평가하고, 우수협력업체를 선정하는데 있다. 협력업체의 평가를 위하여 여러 가지 요인을 분석하여야 하는데, 그런 요인들 중에는 수치적으로 표현 가능한 정량적인 요인이 있는 반면에, 협력업체 제품의 품질이나 재정적 상태와 같은 수치적으로 표현 불가능한 정성적인 요인도 포함되어 있다. 본 논문에서는 정량적인요인과 정성적인 요인을 퍼지추론(Fuzzy Inference)을 통해 분석함으로써 협력업체를 평가하고 최우수 협력업체를 선정하여, SCM환경에서 성공적인 협력관계를 지속적으로 유지할 수 있는 방법론을 제시하고자 한다.

자가 면역성 간염에서 자가항체의 발현 양상과 스테로이드 치료의 효과

박기오,채경훈,허원석,강윤세,정재훈,김연수,문희석,이엄석,김선문,이병석,김남재,이헌영 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

목적 : 최근 8년간 본원에서 경험한 자기면역성 간염의 혈액학적 특징과 스테로이드의 치료효과를 알아보고자 본 연구를 시행하였다. 방법 : 1996년 이후부터 2002년까지 충남대학교 병원에서 1999년 International Autoimmune Hepatitis Group(LAHG)에서 제시한 수정 진단 기준안에 의거하여 총 진단점수가 probable 이상에 해당하는 환자들을 대상으로 임상적 특징, 검사실 및 조직 소견과 스테로이드와 azathioprine의 치료 효과를 후향적으로 조사하였다. 결과 : 9예의 환자들은 여성이 78%, 40대가 많았고, 급성 발병이 33.3%, 피로감, 식욕부진 및 황달 등이 나타났으며, 항핵항체가 55.6%에서 그리고 항평활근 항체가 77.8%에서 양성이었고, 동반 질환은 류마치스 관절염이 2예, 특발성 혈소판 감소증 1예, Henoch-Sch nlein 자반증 및 그레브스병이 각각 1예 씩이었다. 7예에 대해서 스테로이드와 azathioprine으로 치료하여 생화학적 임상적 완해가 이루어져서 유효한 치료 결과를 얻었다. 결론 : 자가면역성 간염은 우리 나라에서 원인을 찾을 수 없는 만성 간질환의 경우, 특히 중년여성에서 다른 자가면역성 질환이 동반되었을 때 자가면역성 간염을 의심하여 자가항체 선별검사를 시행해 보아야 할 것으로 생각된다. 향후 다기관 연구를 통하여 좀 더 많은 증례를 모으고 전향적인 연구를 시행하여야 할 필요가 있다. Although autoimmune hepatitis(AIH) is common in western contries, some studties have been reported in Korea. The aim of the study was to analyze the clinical and histological features and expression pattern of the autoantibody and to examine clinical course of AIH in Korea. For 9 patients diagnosed as having AIH in our hospital since 1996, we reviewed medical records and analyzed clinical and laboratory findings retrospectively. They had a probable or definite AIH according to the revised criteria of International AIH group. The mean age was 42.7(21∼68)years and 7(77.8%) were female. Three of them(33.3%) showed acute hepatitis and five of them showed cirrhosis. Fatigue and anorexia were the most frequently complained symptoms(55.6%). Four patients(44.4%) were accompanied with other autoimmune diseases. Antinuclear antibody was detected in 5(55.6%) and anti-smooth muscle antibody was detected in 7(77.8%). Three patients showed focal picemeal necrosis and four showed periportal fibrosis. Among 7 patients who received immunosuppressive therapy, six patients(85.7%) showed initial response. and biochemical and serologic remission were achived in 5(71.4%) and 3(42.8%) patients, AIH seems to be partly responsible for chronic liver disease of unknown etiology in Korea. AIH should be suspected in female patients with unknown chronic liver disease of unknown etiolgy, especially accompanied with other autoimmune disease. The clinical characteristics of AIH may not be quite different from those of Western countries.

數種의 腐熟劑 添加가 볏짚의 堆肥化에 미치는 影響

尹奉基,金炳浩,金喜權,朴仁珍 목포대학교 자연자원개발연구소 2000 자연자원연구 Vol.3 No.-

This study was conducted to find out effect of microorganisms added for decomposition of organic matter when manufacturing compost. The microorganisms used were EM, LACTO, SEMIP, BAIMUD circulated in market and 068065 developed in Institute of Agricultural Sciences. Effect of microorganisms inoulation on rice straw was summarized as follows: The number of bacteria, actinomycetes and fungi was 28.7∼76.7(×106), 0∼72.0(×106) and 0∼198.7(×105)cfu/g respectively. The number of bacteria, actinomycetes and fungi between microorganisms was no different. The maximum temperature during composting periods was reached 69∼76℃- at 3 50 5 days after heaping and kept over 65℃ for 7 to 10 days. The number of bacteria, actinomycetes and fungi in compost manufactured with rice straw was 42.8∼206.0(×106), 11.3∼54.7(×106), and 9.8∼42.5(×105)cfu/g respectively. The tendency was similar to control. Degree of decomposition of plot added microorganisms except 068065 and BAIMUD was similar to control at 50 days after heaping. The contents of inorganic elements were 1.26∼1.35% T-N, 1.14∼1.38% P2O5, 0.72∼0.97% K2O, 1.01∼1.74 CaO and 0.63∼1.44% MgO. Contents of T-N, P2O5 , CaO, MgO in compost were increased, but content of K2O was decreased, It was no different between treatments.