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BARITUGOKEIANNE,손진아,손유정,김희택,주정찬,최종일,박시재 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.7
Recent environmental problems caused by petroleum-based production of chemicals have accelerated the development of biorefineries for bio-based production of chemicals. Non-pathogenic microorganism, Corynebacterium glutamicum, has extensively been engineered and used as an industrial platform host strain for the commercial production of amino acids, such as L-lysine and L-glutamate. However, only recently has it been developed beyond its use for amino acid production. Recent advances in multiomics approaches, synthetic biology tools and metabolic engineering strategies have enabled the development of recombinant C. glutamicum into a versatile microbial cell factory for biobased production of value-added platform chemicals and polymers by utilization of a broad range of biomass-derived sugars. In this review, we discuss the recent development of synthetic biology tools and techniques used for the enhancement of C. glutamicum’s ability to utilize renewable resources, specifically lignocellulosic biomass, for the production of platform chemicals with C4-C6 carbon backbone such as C4-isobutanol, 2,3-butanediol, C5-itaconic acid, 3- methyl-1-butanol, 2-methyl-1-butanol and C6-muconic acid.
조서영,손유정,박세영,손진아,유지인,BARITUGOKEIANNE,Yokimiko David,강경희,김호용,최종일,이미나,김희택,주정찬,박시재 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.7
Sugarcane molasses was examined for the production of poly(3-hydroxybutyrate) [P(3HB)] and poly(3- hydroxybutyrate-co-lactate) [P(3HB-co-LA)] in recombinant Ralstonia eutropha strains expressing Mannheimia succiniciproducens sacC gene encoding -fructofuranosidase, which can hydrolyze sucrose into glucose and fructose in the culture medium. When crude sugarcane molasses was added to the culture medium to support 20 g/L of sucrose in flask cultivation, the growth of R. eutropha NCIMB11599 expressing the sacC gene was significantly inhibited, which resulted in OD600 of 1.2 with P(3HB) content of 0.1wt%. The inhibition of cell growth due to the usage of the crude sugarcane molasses was relieved by pretreatment of sugarcane molasses with activated charcoal. Sugarcane molasses pretreated with activated charcoal could support the growth of R. eutropha NCIMB11599 expressing the sacC gene to OD600 of 87.2 with P(3HB) content of 82.5 wt% in batch fermentation when it was added to culture medium to support 20 g/L of sucrose. Also, R. eutropha 437-540 expressing Escherichia coli ldhA gene encoding lactate dehydrogenase along with the sacC gene produced P(3HB-co-6.2mol%LA) with 29.1 wt% polymer content from sugarcane molasses in batch fermentation.