족과관절염좌 환자에 대한 동씨침법과 일반침법의 효과에 대한 비교 연구

안호진,정동화,황규선,윤기붕,김태우,문장혁,백종엽,이상무,Ahn, Ho-jin,Jeong, Dong-hwa,Hwang, Kyu-seon,Yoon, Ki-bung,Kim, Tae-woo,Moon, Jang-huyk,Baek, Jong-yeob,Lee, Sang-moo 대한침구의학회 2003 대한침구의학회지 Vol.20 No.1

Objective: The purpose of this study is to compare the effects of Dong-si acupuncture therapy and General acupuncture therapy for the patients with acute ankle sprain. Methods : This study has been carried out for 60 cases of ankle sprain patients who have visited Dong-Seo Oriental medical Hospital from May 1, 2002 to September 30, 2002. We have treated 30 cases of them by Dong-si acupuncture therapy and the other 30 cases by General acupuncture therapy. And we have compared those two group. Results : 1. There was no significant difference at the treatment period and the number of treatment times in comparing two groups. 2. The number of treatment times for good effect is that : Dong-si took $1.57{\pm}0.85$ times and General acupuncture therapy took $2.15{\pm}0.96$ times. And we have found that the effect of Dong-si acupuncture therapy is faster than the other.

연작업자들에서의 δ -aminolevulinic acid dehydratase 유전형질의 다형성이 신기능에 미치는 영향

안익수,황보영,김용배,리갑수,이성수,장봉기,함정오,안규동,이병국 순천향대학교 산업의학연구소 2001 순천향산업의학 Vol.7 No.1

δ-aminolevulinic acid hehydratase (ALAD) is a polymorphic enzyme that has two alleles, ALAD1 (ALAD1-1 as phenotype) and ALAD2 (ALAD1-2 or ALAD2-2 as phenotype). ALAD genotype has been reported to modify the toxicokinetics of lead. To investigate the influence of ALAD genotype polymorphism on renal function in lead workers, author studied 1022 male lead workers and 149 non lead workers in the same industries. For the cross-sectional renal function indices, blood urea nitrogen (BUN), serum creatinine, uric acid and total protein were selected. Blood lead and blood zinc protoporphyrin were also measured as indices of lead exposure. Information on weight, age, job duration, smoking & drinking history were collected. Following results are as follows: 1. Whereas the mean (standard deviation) of blood lead and blood ZPP of lead workers were 27.2±12.5 ㎍/㎗ and 57.7±37.8 ㎍/㎗, those of non-lead workers in the same premises were 14.9±6.5 ㎍/㎗ and 44.6± 15.8 ㎍/㎗ respectively. The difference of mean blood lead and blood ZPP between two groups were statistically significant. 2. Whereas the prevalence of the variant allele, ALAD2 in 1022 lead workers was 10.2 %, the prevalence of that in 149 non-lead workers was 7.4 %. But there was no difference of prevalence between two groups. 3. The mean±standard deviation of blood lead of subjects with ALAD1 was 25.5± 12.7 ㎍/㎗ and was slightly lower than that of subjects with ALAD2 (26.5±10.9 ㎍/㎗), and the mean±standard deviation of blood ZPP of subjects with ALAD1 was slightly higher than that of subjects with ALAD2 (56.7±37.3 ㎍/㎗ vs 48.9±18.6 ㎍/㎗). But the differences of blood lead and blood ZPP between two genotypes were not statistically significant. 4. There were no differences of cross-sectional renal function indices (BUN, serum creatinine, uric acid and total protein) either by lead workers and non-lead workers or two genotypes. 5. In multiple regression analysis of ALAD genotype on BUN after controlling for possible confounders (sex, weight, job duration, smoking and drinking status), the mean of BUN in ALAD2 subjects was 0.629 ㎎/㎗ lower than ALAD1 subjects with a weak statistical significance (p=0.07). But in logistic analysis of ALAD genotype on median value of BUN revcaled that subjects of ALAD2 genotype were 32% less likely to have median value or more of BUN than subjects of ALAD1 with odd ratio of 0.618 (95% confidence interval 0.411-0.920). 6. In multiple regression analysis of ALAD genotype on serum creatinine after controlling for possible confounders (sex, weight, job duration, smoking and drinking status), the mean of serum creatinine in ALAD2 subjects was 0.0271 ㎎/㎗ lower than ALAD1 subjects with statistical significance (p=0.02). On the other hand, in logistic analysis of ALAD genotype on median value of serum creatinine revealed that subjects of ALAD2 genotype were 30% less likely to have median value or more of scrum creatinine than subjects of ALAD1 with odd ratio of 0.700 (95% confidence interval 0.442-1.080). With the above results, it was found that the variant allcle, ALAD2 appeared to modify the association of lead and renal function and ALAD2 genotype may be supportive for the protective effect of lead.

집단 보건관리가 작업장 환경관리개선에 미치는 효과

안규동 가톨릭대학 산업의학 쎈타 산업의학연구소 1989 韓國의 産業醫學 Vol.28 No.4

To evaluate the effect of group health service on the improvement of working conditon over the individual company providing health service, environmintal measurements of work-pace were done in each group of industries. 72 industries in Masan Industrial Complex which belonged to group health service scheme provided by Masan Industrial Health Center, KIHA were selected and 40 industries which were located in Masan and mearby area were selected as a group of company providing health service. The hazardous agents selscted for this study were noise, illumination,dust, organic solvent, acid & alkali, lead & heavy metal. The exposure rate to those hazardous agents were 21.3% in the group of company prov-iding health sevice and 15.6% in group health service respectively. The over rate (no. of measurement above permissible level/total no. of measurement) of those hazardous agents as a whole were significantly lower in group health service(16.3%) than the control group(30.3%) and overall rate of manufacturing industry in Korea(25.5%). Furthermore the over rate of group health service was lower than rate of each type of industries which each industries of group health service were belonged to. With above results we confirmed that group health service was more effective to improvement of working condition than individual company providing health service.

직업적 연폭로가 일부 신경행동학적 검사수행에 미치는 영향

안익수,안현철,김용배,리갑수,김화성,황규윤,최은,장봉기,이성수,안규동,이병국 순천향대학교 산업의학연구소 1999 순천향산업의학 Vol.5 No.1

In order to investigate that blood lead levels of lead exposed workers is associated with decreased performance in neurobehavioral tests in adults, we investigate 78 workers in a battery industry. Three items among neurobehavioral core test battery of World Health Organization, including digit span, Santa ana dexterity, digit symbol were administered to workers occupationally lead exposed workers by operational guide for the WHO neurobehavioral core test battery. Blood lead (PbB) and zinc protoporphyrin in whole blood (ZZP) were selected as an index of lead exposure. Detailed occupational history such as exposed agent, age, work duration, smoking and drinking habits, were recorded as confounding factors. After controlling age, the results of digit span and digit symbol among the neurobehavioral tests were significant contributed by PbB.

한국사람 손등피부신경의 분포 양상

안동춘,황규성,백두진,한승호,정호삼 대한체질인류학회 1999 해부·생물인류학 (Anat Biol Anthropol) Vol.12 No.1

한국사람 손등피부신경의 분포양상을 알아보기 위해 59구(남자 39, 여자 20)의 시체에서 113예(오른손 58/ 왼손 55)의 손등을 해부하여 관찰하였다. 분포유형은 노선경의 분포영역을 기준으로 자신경과의 분포 범위에 따라 구분하여 다음과 같은 결과를 얻었다. 1. 노신경과 자신경으로 이루어진 손등피부신경의 분포양상은 10가지 유형이 관찰되었다. 2. 노신경이 셋째손가락 노쪽절반까지, 자신경이 셋째손가락 자쪽절반까지 이르는 경우(제VIII형)가 33.9%로 가장 많이 관찰되었다. 3. 노신경과 자신경이 셋째손샅에서 혼재하는 경우로, 노신경이 넷째손가락 노쪽절반까지, 자신경이 셋째손가락 자쪽절반까지 이르는 경우(제III형)가 25.7%, 노신경이 셋째손가락까지, 자신경이 셋째손가락 자쪽절반까지 이르는 경우 (제VI형)가 11.0%였다. 4. 노신경과 자신경의 등쪽손가락신경이 셋째손샅에서 합쳐져 하나의 신경가지를 이루는 경우(제IV형)가 12.8%로 나타났다. 5. 두 신경이 둘째손샅과 셋째손샅에서 혼재하거나 둘째손샅에서 합쳐져 분포하는 제III, VI, IX, X, XI 형은 새롭게 관찰된 유형이었다. 6. 근육피부신경이 노신경을 대신하였던 경우는 4가지 유형으로 각각 1예씩 관찰되었다. 7. 손등피부신경의 분포양상을 성별 및 왼손과 오른손에 따라 비교한 결과 통계학적으로 유의한 차이는 없었다. 이상의 결과로 보아 한국인의 손등피부신경은 노신경과 자신경이 셋째손가락 양쪽까지 이르는 경우가 가장 흔한 유형임을 알 수 있었다. In order to investigate the distribution patterns of the dorsal digital nerves of the radial and ulnar nerve in the Korean, authors dissect the 113 hands (right 58/left 55) of the 59 cadavers(39 males/20 females). The types were classified by the area of radial dorsal digital nerves and the ulnar digital nerves. The difference in the distribution pattern between males and females, right and left hands was analysed by x^(2)-test in the case presenting the prequency more than 10%. The results as follows; I. The ten types of the distribution patterns consisted of the radial and ulnar nerves were observed on the dorsum of the hands. 2. The case of the highest prequency was type VIII(33.9%), in which radial nerve supply the radial side of the 2½ of digits and ulnar nerve extends the ulnar side 2½ of digits. 3. In the cases of the both nerve mingling in the third digital web, the incidences in which the radial nerves extend to the radial half of ring finger and ulnar nerve to the ulnar half of middle finger(type III) were 25.7%, and that the radial nerves extend to the ulnar half of middle finger and ulnar nerve to the ulnar half of middle finger(type VI) were 11.0%. 4. Type IV as combined branch between the radial and the ulnar nerve extend to the third digital web was observed in the 12.8%. 5. The type III, VI, IX, X, XI showing the both nerves mingling in the third digital web and in the second digital web or combining in the second digital web were new observed in the Korean. 6. The musculocutaneous nerve replaces the superficial branch of the radial nerve in 4 cases. 7. There was no statistical difference in the distribution pattern between males and females, right and left hands. From the above results, it was suggested that the majority of the cases were that the ulnar digital nerves supplied the ulnar half of the middle finger in the Korean.

漢江上流 人工湖에서의 微生物 分布와 活性度에 관하여

안태석,이동훈,김범철,조규송 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

The values of total hacterial numbers, heterotrophic bacterial numbers, the proportions of specific enzyme releasing bacteria and microbial activities were measured in the five artificial reservoirs in North Han river. The values of total bacterial number ranged from 0.62x10E5cells/ml, which is the common values in mesotrophic lakes. With the effect of civil construction at the upper region of the North Han river, the proportions of alpha-glucosidase-releasing bacteria were higher in Lake Paro and Lake Chuncheon. Beta-glucosidase proportions were high in Lake Paldang and showed high correlation with the concentration of chlorophyll a. The alkaline phophlatase proportion were higher in Lake Paro, Lake Chuncheon and Lake Paldang. The turnover rate of acetate showed no relationship with any other trophic state index intems, such as chlorophyll concentration, phosphate concentration and primary productivity. But the phosphatase activity showed high correlation coefficience, and it should be estimated as a trophic state index in these artificial reservoirs.

표고버섯 약침의 퇴행성 슬관절염 치료효과에 관한 임상연구

황규선,정형섭,백종엽,이상무,한희철,안호진,정동화 대한침구학회 2001 대한침구의학회지 Vol.18 No.6

Objective : This study is designed to find out the effects of Oak-mushroom Herbal acupuncture on osteoarthritis in knee joint. Methods : We investigated 33 cases of patients with osteoarthritis in knee joint that are visited in Hospital of Dong-Seo oriental medicine. We evaluated knee joint function that before and after its treatment by Lysholm score and satisfaction of treatment by Baumgaertner nine point scale and visual analog scale. Results : These results showed that female patients are 26 cases and these are more 19 cases than male patients. On the age of patients, above 61 are mainly distributed as 17 cases. According to Ahlaback's grading system, grade Ⅲ are more than others as 11 cases. We estimated effects of Oak-mushroom Herbal-acupuncture by Lysholm score, those results showed that the mean of Lysholm score before treatment is 51.42, and after treatment is 76.55. That showed statistically meaningful increase. In evaluating of satisfaction, excellent are 11 cases and good are 15 cases. The percentage of above good is 78.8%. Conclusion : According to the results, Oak-mushroom Herbal-acupuncture may be effects on the increase of knee joint function and the decrease of pain in osteoarthritis in knee joint.

백서 혈관평활근 세포에서 α-Lipoic acid가 PAI-1 발현, 세포의 증식, 주유능 및 신생내막 형성억제에 미치는 효과

신동우,이동욱,이상준,김혜순,강효경,안종덕,이인규 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.6

연구배경:당뇨병의 혈관 합병증의 발생에 있어서 산화스트레스는 중요한 역할을 하는 것으로 알려져 있다. 혈관 평활근세포에서 고혈당은 안지오텐신Ⅱ와 더불어 활성산소족(ROS)을 증가시키며, 산화스트레스에 민감한 전사인자들을 활성화시켜 동맥경화증을 유발하는 것으로 알려져 있다. 이에 저자들은 항산화제로 알려진 알파­리포산이 혈관 평활근세포의 증식속도와 이주에 미치는 영향과 PAI­1발현에 미치는 영향을 조사하였고 이와 동시에 백서의 혈관 손상 모델을 이용하여 알파­리포산이 신생내막 증식을 억제시킬 수 있는지를 알아보았다. 방법:In vitro 실험은 백서의 대동맥 평활근세포를 고농도의 포도당(22mM)과 100nM의 안지오텐신Ⅱ로 4시간 배양하였다. 알파­리포산을 처리 후, PAI­1 mRNA의 발현을 보기 위해 노던 블롯을 시행하였고 평활근세포의 유주능과 증식속도에 미치는 영향을 보기 위해 성장속도의 유주능 분석을 시행하였다. 또한 평활근세포의 유주능에 NF­μB 경로가 미치는 영향을 보기 위해 겔 지연 분석과 NF­μB 보고 유전자 분석을 시행하였다. In vivo 실험으로 백서의 혈관손상 모델을 이용하여 알파­리포산 처리 후 혈관의 신생내막 두께를 비교하였다. 결과:In vitro 실험에서 고농도 포도당과 안지오텐신Ⅱ에 의해 유도된 PAI­1 mRNA 발현증가는 알파­리포산 처리에 의하여 용량에 비례하여 억제되었다(p<0.05). 알파­리포산 처리로 혈관 평활근세포의 유주능은 유의하게 억제되었으나(p<0.01),증식속도는 유의하게 억제되지 않았다. 또한 알파­리포산 처리로 NF­μB 발현도 유의하게 억제되었다(p<0.01). In vivo 실험에서 알파­리포산을 주입한 군에서 혈관손상에 의한 신생내막의 증식이 유의하게 억제되는 것을 보여주었다(p<0.01). 결론:알파­리포산은 백서의 대동맥 평활근 세포의 증식속도는 억제하지 못했으나 유주능은 유의하게 억제하였으며, 혈관 손상 모델에서 신생 내막 증식도 유의하게 억제함을 확인할 수 있었다. 이러한 작용은 알파­리포산에 의한 NF­μB 경로의 억제와 연관이 있는 것으로 사료된다. Background : Exposure to large amounts of glucose causes a characteristic dysfunction and morphologic changes of the endothelium by an increased production of reactive oxygen species(ROS) in diabetes. The plasminogen activator inhibitor-1(PAI-1), which modulates fibrinolysis and cell migration may influence proteolysis and neointimal formation in vascular smooth muscle cells(VSMC). Antioxidants have been proposed to inhibit multiple proatherogenic events. This study investigated the effect of α-lipoic acid on PAI-1 expression and VSMC proliferation and migration both in vivo and in vitro. Methods : In the in vitro study, cultured rat aortic smooth muscle cells(RASMC) were incubated in a medium containing high glucose (22 mM) and 100 nM angiotensin Ⅱ for 4 hour. After α-lipoic acidtreatment, a -migration and growth assay of the RASMC, and a gelmobility shift assay and reportergene analysis for nuclear factor- иB(NF- иB) and northern blot analysis for PAI-1 were performed. In the in vivo study, the effect of α-lipoic acid on neointimal hyperplasia in a rat carotid balloon injury model was evaluated. Results : RASMC migration was inhibited significantly by α-lipoic acid (p<0.01), but their prolife ration was not inhibited. The NF-иB DNA binding activity and NF-иB promoter activity was inhibited by α-lipoic acid significantly (p<0.01). α-lipoic acid inhibited PAI-1 mRNA expression by high glucose and angiotensin Ⅱ in dose dependent manner (p<0.05). In the rat carotid artery balloon injury model, neointimal formation was reduced by α-lipoic acid treatment in a dose dependent manner significantly (p<0.01). Conclusion : α-lipoic acid suppresses migration, but not prolife ration in RASMC. α-lipoic acid also reduce neointima formation in a rat carotid balloon injured model. This effect might be related to the blocking of NF-иB which increase the expression of the genes associated with atherosclerosis including TNF-α, IL-1, IL-6, endothelin-1, MCP-1, VCAM-1, ICAM-1, E-selectin, tissue factor(J Kor Diabetes Asso 25:446~459, 2001).

연 작업자들의 업종별 보건관리에 의한 건강수준의 변화

임정규,안규동,이병국 순천향대학교 산업의학연구소 1996 순천향산업의학 Vol.2 No.1

In order to evaluate the effect of industry type specific occupational health service on the health status of lead workers, total 39,203 record of 5 year accumulated data of workers from 1989 to 1993 in lead using industries which made special contract of group occupational health service with Soonchunhyang university, institute of industrial medicine were studied. Blood zinc protoporphyrin (ZPP) and 15 lead related symptoms were selected as a health indices and air concentration of lead (PbA) was selected as a environmental index of lead industries. Blood ZPP and symptoms survey were carried out twice a year for lead workers and once a year for non-lead office workers. Environmental measurements of lead in air were done twice a year in every lead using industries. Lead industries were divided into 4 group of lead industries arbitrarily such as storage battery industries for group I, secondary smelting, litharge making and related operation for group II, telecommunication for group III and primary smelting and crystal glass making for group IV. Data for group III only available for 3.5 years from 1990 to first half of 1993. Blood ZPP was measured with hematoflurometer (Aviv model 206). Fifteen questionnaire of lead related symptoms were provided to all workers and filled by themselves and confirmed by interview doctor at the site. The results obtained were as follows: 1. While the geometric means of lead in air of group I, II, IV in 1989 were 0.333, 0.353 and 0.113 ㎎/㎥, those in 1993 were decreased to 0.081, 0.162 and 0.037 ㎎/㎥ respectively. On the other hand the geometric means of lead in air of group III were always below 0.020 ㎎/㎥ throughout the study period. 2. While the geometric mean of lead in air in medium/large storage battery industries was 0.313 ㎎/㎥ in 1989, it was decreased to below 0.05 ㎎/㎥ since 1992, that in small storage battery industries was 0.395 ㎎/㎥ and it was still 0.101 ㎎/㎥ in 1993. This data suggested that the effect of group occupational health service were more effective in medium/large storage battery industries. 3. The overall percent of distribution of lead in air of below 0.05, 0.05-0.15 and over 0.15 ㎎/㎥ in group I for 5 years were 87.54%, 9.29% and 3.16% respectively. On the other hand, for group II and III, those were 77.19%, 15.21%, 7.6% and 92.78%, 3.72%, 3.5% respectively. The overall percent of distribution of lead in air of above three category in medium/large and small storage battery industries were 90.42%, 7.32%,2.25% and 79.96% 14.47%, 5.57% respectively. 4. The mean blood ZPP of male lead workers decreased from 52.61±68.67 ㎍/100ml in 1989 to 33.31±34.62 ㎍/100ml and those of female lead workers decreased from 84.01±79.06 ㎍/100ml in 1989 to 58.35±47.30 ㎍/100ml. While the mean blood ZPP of male lead workers for group I, II and IV in 1989 was 39.65±40.41, 132.9±121.1 and 32.9±55.51 ㎍/100ml, those in 1993 were 29.75±28.38, 77.35±64.48 and 31.41±21.35 respectively. The mean blood ZPP for group III were ranged from 13.11±8.53 to 26.43±8.83 ㎍/100ml for 3 and half years period. 5. The mean blood ZPP of male lead workers in medium/large and small storage battery industries in 1989 were 32.16, 82.53 ㎍/100ml in 1989 and those in 1993 were decreased to 25.69 and 76.22 ㎍/100ml respectively. Those of female lead workers in both industries in 1989 were 53.36, 126.3 ㎍/100ml and 43.68, 90.17 ㎍/100ml respectively. 6. The overall percent of distribution of blood ZPP of below 50, 50-99, 100-149 and over 150 for 5 years were 72.83% (male: 76.63%, female: 44.59%), 16.97% (male:15.23, femlae: 29.93%), 5.23% (male: 4.18%, female: 13.04%) and 4.96% (male: 3.96%, female: 12.44%) respectively. Among 4 groups, the percent of workers whose blood ZPP over 50 ㎍/100ml was highest in group II. 7. The mean of overall symptoms of male and female lead workers in 1989 were 5.2 and 4.9 and decreased to 4.6 and 4.3 in 1993 respectively. The mean of overall symptoms of male and female lead workers in medium/large storage battery industries in 1989 were 5.7, 5.9 and decreased to 4.8, 5.2 in 1993. Those in small storage battery industries in 1989 werer 4.8, 5.2 and 3.8, 3.2 respectively. 8. The most frequent symptom among 15 lead related symptom questionnaires for 5 year duration was 'generally fatigue' (male: 63%, female: 53%) and the least frequent symptom was 'acute abdominal pain around umbilicus' (male: 12%, femlae: 9%). Among male lead workers of 4 groups the prevalence of lead related symptoms was highest in group 4 and followed by group 3, 1 and 2 in descending order. Among female lead workers it was highest in group 3 and followed by group 1, 2, and 4 in descending order. 9. The prevalence of lead related symptoms were high in group whose blood ZPP below 100㎍/100ml than those with blood ZPP over 100 ㎍/100ml. The prevalence of lead related symptoms of male lead exposed workers were higher or same with male non-lead exposed workers in every symptoms except questionnaire 11. But for female workers the prevalence of lead related symptoms of lead exposed workers were lower than non-exposed lead workers except questionnaire 1, 4, 5, 6, 7, 8, 12, 15. 10. There was very similar pattern of symptom prevalence over the 5 years in lead exposed and non-exposed workers for both sex. 11. The prevalence of lead related symptoms was higher in young age group than old age group and there was no big difference of prevalence according to the work duration. With above results, group occupational health service for lead workers improved working condition of lead industries under special contract with Soonchunhyang university, institute of industrial medicine in term of decrease of lead in air, particularly more effective in medium/large storage battery industries. It also brought out the the decrease of lead burden of most lead workers in terms of blood ZPP of lead workers which is a good index of lead intoxication. But symptom servery did not give any clue of dose-response relationship between lead exposure and lead related symptoms with current symptom questionnaire. It is recommended to revise the way of questioning lead related symptoms from lead workers. On the other hand, for group II and III, those were 77.19%, 15.21%, 7.6% and 92.78%, 3.72%, 3.5% respectively. The overall percent of distribution of lead in air of above three category in medium/large and small storage battery industries were 90.42%, 7.32%,2.25% and 79.96%, 14.47%, 5.57% respectively.

Cluster analysis 에 의한 昭陽湖의 水體 分布

조규송,안태석,김범철,이동훈 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

Cluster analysis was applied for the distinction of the water body in Soyang lake, which is the largest lake in Korea. For this analysis, the sixteen environmental parameters, such as water temperature, nutirent salts, organic materials, ets were used as the input data. The total data matrix was 16parameters X 47 points (in 14 sites). After Standardization, the Euclidean distances between two points were calculate. By the results, main stream of Soyang lake can be classified with 5 groups in March, 10 groups in February. And two shallow tributaries, showed different grouping pattern from main stream. In March, and November, the vertical mixing was detected, and in August, the water body above 10m was showed complex grouping pattern, and showed two water bodies in middle layer, and one in bottom layer.