NEDD4L limits cAMP signaling through ubiquitination of CREB‐regulated transcription coactivator 3

Kim, Yo‐,Han,Yoo, Hanju,Hong, A‐,Reum,Kwon, Minseo,Kang, Sang‐,Wook,Kim, Kyunggon,Song, Youngsup Federation of American Society for Experimental Bi 2018 The FASEB Journal Vol.32 No.7

<P>The transcription factor cAMP-responsive element-binding protein (CREB) is involved in a variety of physiologic processes. Although its activity appears to be largely correlated with its phosphorylation status, cAMP-mediated dephosphorylation and the subsequent nuclear migration of the CREB-regulated transcription factors (CRTCs) are required to stimulate CREB transcriptional activity. Among the 3 identified mammalian homologs of CRTCs, CRTC3 has been shown to be expressed predominantly in adipose tissues in response to catecholamine signals that regulate lipid metabolism. Here, we show that prolonged cAMP signaling down-regulates CRTC3 in a proteasome-dependent manner and that neural precursor cell-expressed developmentally down-regulated gene 4-like (NEDD4L), a specific ubiquitin ligase for CRTC3, is responsible for this process. By recognizing the PY motif of CRTC3, NEDD4L interacts with CRTC3 and promotes its polyubiquitination. Interaction between NEDD4L and CRTC3 is further boosted by cAMP signaling, and this enhanced interaction appears to be dependent on the cAMP-mediated phosphorylation of NEDD4L at the Ser448 site. Furthermore, we show that food withdrawal stimulates NEDD4L phosphorylation in mice, which then show a decrease of adipose tissue CRTC3 protein levels. Together, these results suggest that NEDD4L plays a key role in the feedback regulation of cAMP signaling by limiting CRTC3 protein levels.Kim, Y.-H., Yoo, H., Hong, A.-R., Kwon, M., Kang, S.-W., Kim, K., Song, Y. NEDD4L limits cAMP signaling through ubiquitination of CREB-regulated transcription coactivator 3.</P>

Dependence of the base sequence on the [Cu(2,2′-bipyridine)₂(NO₃)](NO₃)-induced oxidative DNA cleavage probed by linear dichroism

Won, A. Reum,Kim, Raeyeong,Jung, Maeng-Joon,Kim, Seog K.,Lee, Young-Ae Elsevier Sequoia [etc.] 2018 Inorganica chimica acta Vol.471 No.-

<P><B>Abstract</B></P> <P>The oxidative DNA cleavage induced by the [Cu(2,2′-bipyridine)<SUB>2</SUB>(NO<SUB>3</SUB>)]NO<SUB>3</SUB> (Cu(bpy)<SUB>2</SUB>) complex was examined using the linear dichroism (LD) technique. Using this method, the oxidative DNA cleavage by the Fenton mechanism was reported to occur through two sequential first-order reactions. As the Cu(bpy)<SUB>2</SUB> concentration increased, the rate constant of both first order reactions increased, as expected. The activation energy of the second step was estimated to be 0.190 kJ mol<SUP>−1</SUP>. A similar method was applied for various synthetic polynucleotides. Poly[d(G-C)<SUB>2</SUB>], poly(dG)·poly(dC), and poly(dA)·poly(dT) produced a time-dependent decrease in LD, which could be elucidated by a single component exponential decay. This observation is in contrast to Fenton-type oxidative DNA cleavage. On the other hand, poly[d(A-T)<SUB>2</SUB>] produced a time-dependent decrease in the LD magnitude, which could be elucidated by two sequential first order reactions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Cu(bpy)<SUB>2</SUB> complex induced oxidative DNA cleavage. </LI> <LI> The cleavage rate constant for synthetic polynucleotides depended on the nature of base sequence. </LI> <LI> Poly[d(A-T)<SUB>2</SUB>] produced the mechanism similar to native DNA. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Decrease in the LD magnitude at 260 nm for poly(dG)·poly(dC) (curve a), poly[d(G-C)<SUB>2</SUB>] (curve b), poly[d(A-T)<SUB>2</SUB>] (curve c), and poly(dA)·poly(dT) (curve d) at 20 °C.</P> <P>[DISPLAY OMISSION]</P>

상황버섯 추출물의 항산화 및 성분분석

김아름 ( A Reum Kim ),김정은 ( Jung Eun Kim ),박수남 ( Soo Nam Park ) 대한화장품학회 2011 대한화장품학회지 Vol.37 No.4

본 연구에서는 상황버섯 추출물의 항산화 및 항노화 활성 및 항균 효과, 그리고 성분분석에 관한 연구를 수행하였다. 상황버섯 추출물의 자유 라디칼(1,1-diphenyl-2- picrylhydrazyl, DPPH) 소거활성(FSC₅₀)은 에틸아세테이트(ethyl acetate) 분획(2.94 μg/mL)에서 가장 큰 활성을 나타내었고, 루미놀-의존성 화학발광법을 이용한 Fe³⁺-EDTA/H₂O₂ 계에서 생성된 활성산소종(reactive oxygen species, ROS)에 대한 상황버섯 추출물의 총항산화능은 추출물의 에틸아세테이트 분획(0.0072 μg/mL)에서 가장 큰 활성을 나타내었다. 광증감제인 rose-bengal로 증감된 사람 적혈구의 광용혈에 대한 억제 효과를 측정하였을 때 농도범위(5 ~ 50 μg/mL)에서 50 % 에탄올 추출물과 에틸아세테이트 분획 모두 농도·의존적으로 세포 보호 효과를 나타내었다. 타이로시네이즈의 활성 저해 효과(IC₅₀)를 측정한 결과 50 % 에탄올 추출물(IC₅₀ = 6.34 μg/mL)에서 우수한 효과를 나타내었으며, 엘라스테이즈의 활성 저해 효과(IC₅₀)는 에틸아세테이트 분획(IC₅₀ = 14.08 μg/mL)에서 큰 효과가 나타났다. TLC, HPLC 및 LC/ESI-MS를 이용하여 상황버섯 추출물 ethyl acetate 분획의 주성분을 분석하였고 hispidin 유도체인 interfungin A를 확인하였다. 이상의 결과들은 상황버섯 추출물이 ROS에 대항하여 세포막을 보호함으로써 생체계, 특히 태양 자외선에 노출된 피부에서 항산화제로써 작용할 수 있으며, 특히 상황버섯 추출물의 에틸아세테이트 분획을 항산화, 항노화 및 미백 기능성 화장품 소재로써의 응용 가능성을 확인하였다. In this study, the antioxidative effect, antibacterial, inhibitory effects on tyrosinase, inhibitory effects on elastase and component analysis of Phellinus linteus (P. linteus) extracts were investigated. The ethyl acetate fraction of P. linteus extracts (2.94 μg/mL) showed the highest free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (FSC₅₀). Reactive oxygen species (ROS) scavenging activity (OSC₅₀) of P. linteus extracts on ROS generated in Fe³⁺-EDTA/H₂O₂ system was investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate fraction (0.0072 μg/mL) showed the most prominent ROS scavenging activity. The protective effects of extract/fractions of P. linteus extracts on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The P. linteus extracts showed cellular membrane protective effects in a concentration dependent manner (5 ~ 50 μg/mL). The inhibitory effect (IC₅₀) on tyrosinase of P. linteus extract was the highest at 50 % ethanol extract (6.34 μg/mL), and the inhibitory effect (IC₅₀) on elastase of P. linteus was the highest at ethyl acetate fraction (14.08 μg/mL). TLC, HPLC chromatogram and LC/ESI-MS of the ethyl acetate fraction obtained from P. linteus extracts were identified interfungin A (PL RPT-1a). These results indicate that extract/fractions of P. linteus can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging ROS, and protect cellular membranes against ROS. Extract/fractions of P. linteus can be applicable to new cosmeceuticals for antioxidant, antiaging, antiwrinkle and whitening.

지황 ‘토강’의 연작 시 근경 잔사물과 토양 훈증처리가 생장, 뿌리썩음병 및 수량에 미치는 영향

권아름(A Reum Kwon),김선익(Sun Ick Kim),성봉재(Bong Jae Seong),곽성희(Sunghee Guak) 한국약용작물학회 2023 한국약용작물학회지 Vol.31 No.6

Background: The study aimed to explore the impact of rhizome residues and soil fumigation on the growth, root rot incidence and yield of Rehmannia glutinosa ‘Togang’ during successive cropping. Methods and Results: In mid May 2019, two types of rhizome residues, healthy and diseased, were incorporated as a powder into fresh soil in plastic containers at different concentrations, including 0, 400, 800 or 1,600 g / 200ℓsoil. Additionally, the soil was fumigated with dazomet at 0, 30, 40 and 50 ㎏ per 10 a before planting. For both experiments, the effects on shoot growth, rhizome yield and root rot incidence were evaluated. The results show that the presence of rhizome residues in the field, particularly diseased ones, substantially reduced the shoot growth and rhizome yield in R. glutinosa ‘Togang’, along with a slight increase in root rot incidences, with the effect being greater with an increasing amount of the residues incorporated. Soil fumigation with dazomet was able to effectively increase shoot growth and rhizome yield but decrease root rot in ‘Togang’ under successive cropping. The recommended application rate was found to be 40 ㎏ per 10 a, where rhizome yield was substantially increased by about 61%. Conclusion: Soil fumigation with dazomet was quite effective in alleviating the negative effect of successive cropping on shoot growth, yield and root rot in R. glutinosa ‘Togang’, especially when applied at the rate of 40 ㎏ per 10 a.

Clinical Characteristics and Metabolic Features of Patients with Adrenal Incidentalomas with or without Subclinical Cushing’s Syndrome

Bo-Yeon Kim,A-Reum Chun,Kyu-Jin Kim,Chan-Hee Jung,Sung Koo Kang,Ji-Oh Mok,Chul-Hee Kim 대한내분비학회 2014 Endocrinology and metabolism Vol.29 No.4

Background: The aim of this study was to examine the clinical characteristics of adrenal incidentalomas discovered by computed tomography (CT) and to investigate metabolic features of subclinical Cushing’s syndrome (SCS) in patients with adrenal incidentalomas in a tertiary hospital in Korea. Methods: This retrospective study examined the clinical aspects of 268 patients with adrenal incidentalomas discovered by CT at Soonchunhyang University Bucheon Hospital. Clinical data and endocrine function of the patients as well as histological findings were obtained from medical records, while anatomic characteristics were analyzed by reviewing imaging studies. Hormonal tests for pheochromocytoma, Cushing’s syndrome, and aldosterone-secreting adenoma were performed. Results: Most (n=218, 81.3%) cases were nonfunctioning tumors. Of the 50 patients with functioning tumors (18.7%), 19 (7.1%) were diagnosed with SCS, nine (3.4%) with overt Cushing’s syndrome, 12 (4.5%) with primary aldosteronism, and 10 (3.7%) with pheochromocytoma. Malignant tumors (both primary and metastatic) were rare (n=2, 0.7%). Body mass index, fasting glucose, hemoglobin A1c, and total cholesterol were significantly higher in patients with SCS in comparison with those with nonfunctioning tumors. The prevalence of type 2 diabetes mellitus and hypertension were significantly higher in patients with SCS compared with those with nonfunctioning tumors. Conclusion: Functioning tumors, especially those with subclinical cortisol excess, are commonly found in patients with adrenal incidentalomas, although malignancy is rare. In addition, patients with SCS in adrenal incidentalomas have adverse metabolic and cardiovascular profiles.

Productivity Tests on Selected panax ginseng C. A. Meyer Lines

Bong Jae Seong(성봉재),Sun Ick Kim(김선익),Ka Soon Lee(이가순),Moo Geun Jee(지무근),Su Dong Kim(김수동),A Reum Kwon(권아름),Hyun Ho Kim(김현호),Jin Woong Cho(조진웅),Jun Yeon Won(원준연) 한국약용작물학회 2018 한국약용작물학회 학술대회논문집 Vol.2018 No.1

인삼 선발계통의 지역적응성 검정

Bong Jae Seong,Sun Ick Kim,Ka Soon Lee,Moo Geun Jee,Su Dong Kim,A Reum Kwon,Hyun Ho Kim1,Young Chang Kim 한국약용작물학회 2017 한국약용작물학술대회 발표집 Vol.2017 No.05

Background : For cultivation of varieties of ginseng, the pure line selection method, which is to select the best among those cultivated in farms for pedigree breeding, replicated yield trials and regional adaptation trials before registering as a new variety, is widely used. Although there are 25 registered varieties of ginseng in Korea, the quality of ginseng is declining together with the amount of harvest being decreased by 15 - 20% due to the heat injuries and diseases from the warming & abnormal climate. Thus, the needs for development of disaster-resistant varieties with better chances of surviving through high temperature, salts and disease are increasing. Therefore, this study is to cultivate disaster-resistant varieties among those selected for their disaster tolerance and salt tolerance through regional adaptation trials. Methods and Results : As a result of examining the growth characteristics of the selected 2 - 5 year old varieties used in the study, among the 5-year old crops, Goryeo 4 and Eumseong 5 showed superior growth in both above and below aerial parts, and among the 4-year old crops, Eumseong 11 and Cheonryang showed superior growth while the growth in the below aerial parts were satisfactory in the order of Cheonryang > Eumseong 10 > Eumseong 11 > Eumseong 9. Among the 3-year old crops, the most superior growth in both above and below aerial parts was observed in Eumseong 14 with the weight of the below aerial part, root diameter and taproot length at 13.8 g, 11.8 ㎝ and 6.2 ㎝ respectively. Among the 2-year old crops, Eumseong 10 showed the most superior growth in both above and below aerial parts. Conclusion : Based on the above results, Goryeo 4 and Eumseong 5 among the 5-year old crops, Eumseong 11 among the 4-year old crops, Eumseong 14 among the 3-year old crops and Eumseon 10 among the 2-year old crops showed the most superior growth among the selected varieties. The growth characteristics of both above and below aerial parts in each year will continuously be monitored.

첨가 방법을 달리하여 제조한 아로니아 막걸리의 이화학적 특성 및 항산화 활성

이아름,오은영,정연정,노재관,윤향식,이기열,김이기,엄현주,Lee, A Reum,Oh, Eun Young,Jeong, Yeon Jeong,Noh, Jae-Gwan,Yoon, Hyang-Sik,Lee, Ki Yeol,Kim, Yee Gi,Eom, Hyun-Ju 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.4

Aronia melanocarpa (Rosaceae family), black chokeberry, has significantly higher anthocyanin, phenolic compounds, and antioxidant activity than other berries. The aim of this study is to characterize the content of aronia makgeolli prepared with the additive method such as fresh-type and crushed-type and to investigate the effect of aronia on the quality of makgeolli at $25^{\circ}C$ for 8 days. The changes in pH, total acidity, ethanol content, color, antioxidant activity, total polyphenol content, and sensory testing were determined. The pH values were 3.9~4.2 in fresh aronia makgeolli (the fresh group), and 3.6~3.9 in crushed aronia makgeolli (the crushed group). The total acidity of all samples gradually increased during the fermentation period. The ethanol content was 10~15% after the second stage of fermentation, and was reduced followed by an increase in the addition rate of aronia, showing the lowest values in 50% crushed aronia makgeolli. As the aronia content increased, both the antioxidant activity and the total polyphenol content significantly increased. In the fresh group, low values were seen in early fermentation, which significantly increased according to the progress of fermentation, while the crushed group showed high values for 6 days. In the sensory evaluation, the color, flavor, bitterness, and overall acceptance of the makgeolli with 30% fresh aronia showed higher values than the control and other samples. In conclusion, the present study suggests that fresh aronia without undergoing the breaking process is a more suitable additive method than the crushed type for antioxidant activity and palatability of aronia makgeolli. 본 연구는 막걸리 제조 시 아로니아의 첨가 방법을 달리하여 제조한 후, 발효하면서 항산화 활성 및 이화학적 품질특성을 분석하였다. 아로니아는 생과와 마쇄한 형태로 첨가하였고, 쌀 함량의 10%, 30% 그리고 50%로 하였다. 막걸리의 pH는 발효 초기에 생과를 첨가한 시료가 3.9~4.2로, 마쇄하여 첨가한 시료(3.6~3.9)보다 높았고, 발효가 진행되면서 생과로 첨가한 경우는 pH가 낮아지다가 다시 증가하는 경향성을 보였다. 총산의 경우는 초기 발효에는 생과보다 마쇄한 시료에서 더 높게 나타났고, 발효가 진행될수록 총산의 함량이 증가하였다. 환원당 함량은 발효가 진행되며 대부분의 시료에서 감소하였다. 에탄올 함량은 1단 담금 시 11.4%로 나타났고, 2단 담금 6일 후에는 10~15%였으며, 특히, 아로니아를 50% 첨가한 실험구는 1단 담금 시 보다 낮은 에탄올 함량을 보였으며, 마쇄하여 첨가할수록 더 낮은 알코올함량을 보였다. 색도 중 a값(적색도)을 비교했을 때 생과 사용 시 천천히 a값이 높아져 마지막 날에는 생과 처리구 간 유의적으로 차이가 났지만, 마쇄한 경우는 처음부터 마지막 발효까지 높은 a값을 가져 생과를 처리한 경우보다 높게 나타났다. 총 폴리페놀함량 측정과 DPPH radical 소거능 측정 결과는 아로니아 첨가량이 증가할수록 함께 증가하였는데, 생과첨가군의 경우 초기에는 낮은 값을 가지다가 발효가 진행하며 급속히 증가하였고, 마쇄첨가군에서는 초기에서부터 높은 값을 가졌다. 또한 아로니아를 30% 첨가하여도 50%를 첨가한 것과 같이 뛰어난 항산화 활성을 나타내었다. 관능검사는 생과 30%를 첨가한 시료가 색, 쓴맛, 전체적 기호도에서 높아 관능적 만족도가 우수한 것으로 나타났다. 이상의 결과로 아로니아를 첨가한 기능성 막걸리의 특성을 보았을 때 아로니아를 마쇄한 형태보다 생과 형태로 30%를 첨가하는 것이 관능적 특성과 항산화 활성을 고려하였을 때 가장 적합한 것으로 나타났다.

Analysis for the Genetic Similarity of Native Ginseng (Panax ginseng C. A. Meyer) Collections in Geumsan Using DNA Markers

Moo Geun Jee(지무근),Bong Jea Seong(성봉재),Sun Ick Kim(김선익),Ka Soon Lee(이가순),Su Dong Kim(김수동),A Reum Kwon(권아름),Hyun Ho Kim(김현호) 한국약용작물학회 2017 한국약용작물학회 학술대회논문집 Vol.2017 No.1

The Role of Plant Extracts in Alleviating Particulate Matter-induced Inflammation in Barrier-interrupted Skin

( Ji Yeon Hong ),( Sun Young Choi ),( Jae Wan Park ),( Jin O Park ),( A Reum Kim ),( Beom Joon Kim ) 대한피부과학회 2021 大韓皮膚科學會誌 Vol.59 No.3

Background: The adverse effects of particulate matter (PM) on the skin have been concerning recently. Objective: We investigated PM induced inflammation on barrier-interrupted skin and relieving effects of plant extracts on PM-induced skin irritation. Methods: A total of 20 participants were enrolled for the induction of skin irritation. Sodium lauryl sulfate (SLS) patch was affixed to the forearm for 24 hours. After patch removal, total suspended particulates (TSPs) were applied. After inducing skin irritation using SLS and TSP, cream containing 0.1% mixture of plant extracts (Citrus sunki, Sceptridium ternatum (Botrychium ternatum), and Korthalsella japonica) was applied. The a* value, erythema index (EI), and transepidermal water loss (TEWL) were measured. Results: The a* value was significantly higher at the site where TSPs were applied than that at the site where TSPs were not applied at 1 and 2 days after SLS patch removal. The a* value, EI, and TEWL were significantly low in the cream-applied area 7 days after the introduction of the SLS patch and TSPs. Conclusion: PM induced skin irritation in a previously disrupted skin barrier. Topical application of plant extracts alleviated skin irritation symptoms caused by dust exposure. (Korean J Dermatol 2021;59(3):217∼224)