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온실에서 동양란인 심비디움 재배에 공생균의 효과에 관한 연구
이상선,이준기,이정우,박소영,백기엽 충북대학교 첨단원예기술개발연구센터 2000 연구보고서 Vol.5 No.-
The orchid symbiotic fungus (Tulsanella repens P-01; OSF) was cultured on the soil materials by solid culture and inoculated in the two varieties of GwanUm and SaGae of Cymbidium hybrids with the barks. The two varieties of the oriental orchid were measured after 12 months' cultivation under the conditions of greenhouse; fresh weight, numbers of bulb, and rate of dead bulb for an individual plant. The fresh weight of plants were measured to be different for the individual plant. The fresh weight of plants were measured to be different for the individual plants treated with the OSF or not, depending on the varieties, but the rates of dead bulb were significant, disregarding with the varieties. The roots of the plants treated were observed to be more health and heavier weight than those not treated. The bulbs of the plants not treated were measured to be dead at the rate of 20% for a year's cultivation. The roots of two varieties were also observed by naked eyes and by microscope; Several peletons were observed to be in the root cells in the individual plants treated with OSF, but not in the individual plants treated without the OSF. Further, the pathogenic fungal hyphae were observed to be in the roots under the dead bulbs of both varieties not treated; appessorium or hasutorium on the epidermal cells of root. The inoculation of OSF was speculated to stimulate the growth of plants by absorption of mineral nutrition and defense the invasions of soil pathogenic fungi for the cultivations of Cymbidium hybrids.
식이섬유질원이 포함된 식이가 흰쥐의 장내 균총조성에 미치는 영향
이상선,이현아,김연희 漢陽大學校 韓國生活科學硏究所 1996 韓國 生活 科學 硏究 Vol.- No.14
This study was performed to investigate the influence of dietary fiber from the whole food on the composition of intestinal microflora in rats. The fiber sources of experimental diets were prepared by drying and milling of small water dropwort(WD), mugwort(MW), butterbur(BB) and apple(AP). Each fiber sources was mixed at the 15% level of the diet. The level of dietary fiber in WD, MW, BB diets was simillar but that in AP diet was slightly low. Male rats of Sprague-Dawley strain were blocked into 5 groups: WD, MW, BB, AP, FF(fiber free), The animal was fed ad libitum each of experimental diets for 37 days. Total viable counts and the numbers of Lactobacillus, Bifidobacterium, Bacteroidaceae, Eubacterium, Peptococcaceae, Streptococcus, Clostridium, Staphylococcus, E. coli were determined by nonselective and various selective media. Food intake of AP and FF groups were lower than the other groups, FF group was lower than the other groups in flood efficiency ratio. Total viable counts were the highest in FF group. The number of Bacteroides, Eubacterium were higher in AP group compared with other groups and that of streptococcus was lower in MW, BB, AP groups than others. The number of Lactobacillus was higher in WD, MW, BB groups than the other groups and that of Bifidobacterium was higher in BB, AP groups. A decrease in the intestinal population of Clostridium was shown in MW, BB groups. These findings suggests that the samll water dorpwort, mugwort butterbur are effective in improving intestinal flora of rats.