http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
미국 임상병리검사소 법제에 관한 연구(Ⅱ) : 실력데스트 및 정도관리를 중심으로
이창규,이승관,박종성,정수경,황선철 高麗大學校 倂設 保健大學 保健科學硏究所 1995 保健科學論集 Vol.21 No.1
With the passage of the Clinical laboratory Improvement Amendments of 1988, Congress has profoundly influenced laboratory testing practices in the U. S. Media stories leading to the passage of CLIA '88 raised the public's concern about inaccurate and imprecise results for certain laboratory tests. CLIA's main areas of interest and controversy include the effects on health care of the personnel standards and test complexity categories, and the costs of laboratory certification, proficiency testing, and quality control measures. The conventional approach to clinical testing standards seeks to assure quality by regulating the laboratory analytical process. However, little empirical evidence is available to support or refute this model, which has been used during the past 25years. The goal is to create a regulatory framework that is not burdensome, incorporating equitable requirements and maintaining timely access to testing at a reasonable cost while ensuring that all laboratories have personnel with the technical ability and skills to provide quality results.
중합효소 연쇄반응과 제한효소 분석법을 이용한 Mycobacteria 균종의 신속한 동정
이창규,이갑노 고려대학교 의과대학 1995 고려대 의대 잡지 Vol.32 No.1
The proportion of nontuberculous mycobacteria infection is increasing, but identifying mycobacteria species by conventional methods requires at least 2 or 4 weeks because of their slow growth rate. Two-step assay combining polymerase chain reaction(PCR) and restriction enzyme analysis was developed to differentiate the mycobacteria species. These species were M. avium, M. bovis, M. fortuitum, M. gordonae, M. intracellulare, M. kansasii, M. phlei, M. scrofulaceum, M. segmatis, M. terrae, M. tuberculosis, and M. vaccae. With PCR, 439-bp portion of the gene coding for the 65 kDa protein was amplified. 65 kDa protein contains epitope unique as well as common to various species of mycobacteria. The PCR product was digested with 4 restriction enzymes: Sma Ⅰ, Alu Ⅰ, Msp Ⅰ, and Hae Ⅲ. The restriction enzyme analysis patterns of standard mycobacteria were analyzed. The method was applied to 31 clinical isolates. The results and conclusions were as follows: 1. Diagnostic algorithm could be made with Msp ⅠI and Hae Ⅲ restriction analysis patterns that had a higher discrimination capacity. 2. All of 31 clinical isolates could be identified to the species within 2 days except 4 cases of complex form mycobacteria. This method is so simple and rapid that it can be used as a routine test in clinical laboratory. Early identification of mycobacteria can help physician to decide the therapeutic regimen and the prognosis of patient.