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Real-time PCR을 이용한 환경 중 물 시료의 레지오넬라 분석법 연구
이정희,이정희,박명기,김윤성,윤희정,이창희,정아용,윤미혜 한국환경보건학회 2019 한국환경보건학회지 Vol.45 No.5
Objectives: The standard method for the enumeration of environmental Legionella is culturing, which has several disadvantages, including long incubation and poor sensitivity. The purpose of this study is to demonstrate the usefulness of real-time PCR and to improve the standard method. Methods: In 200 environmental water samples, a real-time PCR and culture were conducted to detect and quantify Legionella. Using with the results of the survey, we compared the real-time PCR with the culture. Results: Each real-time PCR assay had 100% specificity and excellent sensitivity (5 GU/reaction). In the culture, 36 samples were positive and 164 samples were negative. Based on the results of the culture, real-time PCR showed a high negative predictive value of 99%, 35 samples were true positive, 105 samples were true negative, 59 samples were false positive and one sample was a false negative. Quantitative analysis of the two methods indicated a weak linear correlation (r 2 =0.29, r 2 =0.61, respectively). Conclusions: Although it is difficult to directly apply quantitative analysis results of real-time PCR in the enumeration of environmental Legionella, it can be used as a complementary means of culturing to rapidly screen negative samples and to improve the accuracy of diagnosis.
이정희,김영희,정태호 대한마취과학회 1982 Korean Journal of Anesthesiology Vol.15 No.2
We have had a case of unilateral vocal cord paralysis following endotracheal anesthesia for abdominal surgery. Electromyographic examination of the intrinsic laryngeal muscle to study the nerve injury was not done but we have considered that the recurrent laryngeal nerve was injured following endotracheal anesthesia according to the post-operative laryngeal view and recovery progress. Some factors of nerve injury such as overrotation of the neck during the operation, pressure on the nerve by an overexapanded cuff, vulnerability and the anatomy of the nerve were all deemed to do possible causes, and we considered the interaction of this combination responsible for the nerve injury. The recovery were relatively good and healed within 80days after operation.
A Study on the Incorporation of Sulfate-sulfur into Sulfur-containing Protein
이정희,전세열,신상주,Lee, Chung-Hee,Chun, Sea-Yull,Shinn, Sang-Joou 생화학분자생물학회 1971 한국생화학회지 Vol.4 No.1
고등동물에 있어서 황산염 유황의 단백질 전환은 소화관내에 기식하는 미생물의 도움으로만 가능한 것으로 알려져 있으나 본 실험에서는 고등 동물자체가 독자적으로 그와 같은 기능을 가졌는가를 구명하고져 반추초식덩물 아닌 토끼를 이용하여 표지된 황산염을 여러가지 방법으로 투여하여 그것이 단백질 합성에 이용됨을 구명하였고 토끼가 그와 같은 능력을 독자적으로 지녔음을 확인하였다. 각 기관에 있어서의 방사능 분포 상태도 아울러 조사하였다. Hinted by a symbiotic relationship between rumen microorganisms and host ruminant in utilization of inorganic sulfur compounds such as sodium sulfate and ammonium sulfate, an investigation was conducted to determine a similar symbiotic effect between ceacum microflora and host rabbit, for ammonium sulfate. Radioactivity of fecal emission was detected from the first day of administration but the radioactive compound(s) was not precipitated by barium chloride. A similar fact was found when the labelled sulfate-sulfur was administered by intraperitoneal injection. When urinary ejection was fractionated into 3 parts, namely, trichloroacetic acid precipitate, barium chloride precipitate and supernatant fractions, radioactivity was almost equally distributed into barium chloride precipitable and supernatant fractions with a minor beta-ray emission from TCA precipitated fraction. Radioactivity was detected in hairs of experimental subjects regardless the mode of drug administration. Our finding shows that angola rabbit possesses per se the capacity of such utilization independent to its ceacum microflora. Liver homogenates made 48 hours after the suspension of drug administrations showed no radioactive emission from its barium chloride precipitated fracton. Majorities of beta-ray emission was detected in the TCA precipitated fraction. A minor amount of count, detected from supernatant is interpreted as an evidence that in the liver, transformation of inorganic sulfate sulfur into other sulfur compound (s) is a pre-requisite for protein incorporation or other physiological or metabolic process. The results of in vitro test for microbial incorporation of labelled sulfate-sulfur show that when ceacum microfiora are incubated under anaerobic conditions the microorganisms can incorporate the labelled element into their cellular protein. The capacity of doing so seems to be increased by a prolonged exposure to or contact with such compound expressed otherwise, they can be trained to do so.