양파(Allium cepa L.) 유전자원의 Flavonol 배당체 조성 및 함량

이 정로,이 민기,김 헌웅,이 성현,이 영민,장 환희,황 경아,곽 재균,고 호철,김 정봉,조 진웅 한국작물학회 2014 Korean journal of crop science Vol.59 No.3

Flavonols as a major kind of plant secondary metabolites are known for health-promoting compounds in onions (Allium cepa L.). The objectives of this study are to determine profiles of flavonol glycosides in different 75 onion accessions. A total of five flavonols (quercetin 3,4'- diglucoside, Q34'diG; quercetin 3-glucoside, Q3G; quercetin 4'-glucoside, Q4'G; isorhamnetin 4'-glucoside, I4'G; quercetin, Q) were identified from onion accessions. In positive ion mode using LC-ESI-MS, individual flavonols were confirmed from one and two glycosylation binding with aglycone such as quercetin and isorhamnetin. Total flavonol contents were distributed in white onion (range of 0.18-6.47 mg/g DW) and purple onion accessions (range of 2.39-6.47 mg/g), respectively. The mean of flavonol contents in purple onion (4.41 mg/g) showed 1.4-fold higher than white onion (3.23 mg/g). The Q34'diG and Q4'G were considered as the major compounds of flavonol glycosides in onion accessions.

유해남조류 발생 잠재성 분석을 위한 eDNA 기반의 퇴적물 전처리 방법: 밀도 구배 원심분리법(Ludox method)

유경은,호혜인,김현진,김건희,황순진 한국하천호수학회 2023 생태와 환경 Vol.56 No.1

Environmental DNA (eDNA) can exist in both intracellular and extracellular forms in natural ecosystems. When targeting harmful cyanobacteria, extracellular eDNA indicates the presence of traces of cyanobacteria, while intracellular eDNA indicates the potential for cyanobacteria to occur. However, identifying the “actual” potential for harmful cyanobacteria to occur is difficult using the existing sediment eDNA analysis method, which uses silica beads and cannot distinguish between these two forms of eDNA. This study analyzes the applicability of a density gradient centrifugation method (Ludox method) that can selectively analyze intracellular eDNA in sediment to overcome the limitations of conventional sediment eDNA analysis. PCR was used to amplify the extracted eDNA based on the two different methods, and the relative amount of gene amplification was compared using electrophoresis and Image J application. While the conventional bead beating method uses sediment as it is to extract eDNA, it is unknown whether the mic gene amplified from eDNA exists in the cyanobacterial cell or only outside of the cell. However, since the Ludox method concentrates the intracellular eDNA of the sediment through filtration and density gradient, only the mic gene present in the cyanobacteria cells could be amplified. Furthermore, the bead beating method can analyze up to 1 g of sediment at a time, whereas the Ludox method can analyze 5 g to 30 g at a time. This gram of sediments makes it possible to search for even a small amount of mic gene that cannot be searched by conventional bead beating method. In this study, the Ludox method secured sufficient intracellular gene concentration and clearly distinguished intracellular and extracellular eDNA, enabling more accurate and detailed potential analysis. By using the Ludox method for environmental RNA expression and next-generation sequencing (NGS) of harmful cyanobacteria in the sediment, it will be possible to analyze the potential more realistically.

퇴적층 남조류 휴면세포의 생리적-분자생물학적 연구를 위한 Ludox 처리법

김건희,유경은,호혜인,박채홍,김현진,황순진 한국하천호수학회 2023 생태와 환경 Vol.56 No.1

Cyanobacterial resting cells, such as akinetes, are important seed cells for cyanobacteria’s early development and bloom. Due to their importance, various methods have been attempted to isolate resting cells present in the sediment. Ludox is a solution mainly used for cell separation in marine sediments, but finding an accurate method for use in freshwater is difficult. This study compared the two most commonly used Ludox methods (direct sediment treatment and sediment distilled water suspension treatment). Furthermore, we proposed a highly efficient method for isolating cyanobacterial resting cells and eDNA amplification from freshwater sediments. Most of the resting cells found in the sediment were akinete to the Nostocale and were similar to those of Dolichospermum, Cylindrospermum, and Aphanizomenon. Twenty times more akinetes were found in the conical tube column using the sediment that had no treatment than in the sample treated by suspending the sediment in distilled water. Akinete separated through Ludox were mainly spread over the upper and lower layers in the column rather than concentrated at a specific depth in the column layer. The mibC, Geo, and 16S rDNA genes were successfully amplified using the sediment directly in the sample. However, the amplification products of all genes were not found in the sample in which the sediment was suspended in distilled water. Therefore, 5 g to 10 g of sediment is used without pretreatment when isolating cyanobacterial resting cells from freshwater sediment. Cell isolation and gene amplification efficiency are high when four times the volume of Ludox is added. The Ludox treatment method presented in this study isolates cyanobacterial resting cells in freshwater sediment, and the same efficiency may not appear in other biotas. Therefore, to apply Ludox to the separation of other biotas, it is necessary to conduct a pre-experiment to determine the sediment pretreatment method and the water layer where the target organism exists.

비스포스포네이트 관련 악골괴사에 대한 후향적 연구

한윤식(Yoon-Sic Han),이인우(In-Woo Lee),이 호(Ho Lee),서진원(Jin-Won Suh),김성민(Soung-Min Kim),명 훈(Hoon Myoung),황순정(Soon-Jung Hwang),최진영(Jin-Young Choi),이종호(Jong-Ho Lee),정필훈(Pill-Hoon Choung),김명진(Myung-Jin Kim),서병 대한구강악안면외과학회 2011 대한구강악안면외과학회지 Vol.37 No.6

Introduction: The incidence of bisphosphonate-related osteonecrosis of the jaw (BRONJ) has increased gradually in patients who have undergone surgical treatment for osteomyelitis. In this study, a retrospective analysis of BRONJ patients was carried out using the data of osteomyelitis patients treated surgically. Materials and Methods: Osteomyelitis patients, who underwent curettage, sequestrectomy, saucerization or decortications, and partial mandibulectomy at Seoul National University Dental Hospital from 2004 to 2010 were enrolled in this study. The patients were classified and categorized into two groups based on the surgical records and progress notes. One group comprised of patients with osteomyelitis and osteoporosis, and the other group included patients with osteomyelitis only. The epidemiological data of the BRONJ patients was analyzed to identify any trend in the incidence of BRONJ in osteomyelitis patients. Results: Among 200 patients who underwent surgical intervention for osteomyelitis, 64 (32.0%) were identified as having osteoporosis as the underlying disease. In these 64 patients, more than 81.3% had been prescribed bisphosphonates. Females were far more affected by BRONJ than males. The incidence of BRONJ also increased with age. The posterior part of the mandible was affected more frequently by BRONJ. Conclusion: Although the availability of potent antibiotics and increased oral hygiene care can reduce the overall incidence of osteomyelitis, BRONJ can increase the total incidence. To prevent BRONJ, it is recommended that an oral examination be performed before prescribing bisphosphonates. Moreover, the patients should be educated about the potential risks of dental procedures that might be causal factors for BRONJ. Furthermore, patient swho take bisphosphonates for the treatment of osteoporosis should undergo periodic follow up oral examinations to prevent BRONJ.

전신성 홍반성 루프스를 동반한 임산부의 질병활성 지수로 이용되는 C3, C4, 항dsDNA항체, autoimmune target test와 임신 합병증들과의 상관관계

김일동 ( Il Dong Kim ),김라현 ( Ra Hyun Kim ),강귀언 ( Gui Un Kang ),진성아 ( Sung Ah Jin ),이혜진 ( Hye Jin Lee ),호정규 ( Jeong Kyu Hoh ),장호선 ( Ho Sun Chang ),황경진 ( Kyung Jin Hwang ),박문일 ( Moon Il Park ) 대한산부인과학회 2011 Obstetrics & Gynecology Science Vol.54 No.1

목적: 루푸스가 합병된 임산부에서 임신 결과와 양상, 또한 임신 중 루푸스의 활성도를 알 수 있는 C3/C4보체, 항dsDNA, autoimmune target test (AITT) 수치를 비교 분석하여 루푸스 산모와 신생아의 예후와의 상관관계를 알아보았다. 연구방법: 2001년 1월부터 2008년 12월까지 한양대학교 의료원 산부인과와 류마티스 병원에서 임신을 경험한 환자를 대상으로 임신 경과와 루푸스 병력 기록이 추적 가능한 73명의 93예를 대상으로 후향적으로 조사하였다. 임신의 임상 경과와 결과, 산과적 합병증, 임신중 루푸스의 경과, 루푸스에 의한 산모 질환, 임신 전후 복용한 약물, 신생아의 예후에 대해 조사하고, 혈액학적, 면역학적 검사를 하였다. 통계분석은 SPSS ver. 14.0을 사용하여 chi-square검정과 피어슨 상관 계수를 사용하여 분석하였다. 결과: 보체 C3 수치와 관련된 항목을 보면 산모의 백혈구 감소, 혈청 C-reactive protein (CRP) 증가, 혈뇨, 고혈압, 만삭 전 조기 양막파열 등과 연관성을 보였고, 보체 C4 수치는 산모의 단백뇨, 혈뇨, 혈액학적 질환, 신생아 중환자실 입원과 연관성을 보였다. 항dsDNA는 산모의 혈청 CRP 증가, 양수과소증, 신생아 anti-Sj?gren``s syndrome B (La) antibody와 연관성을 보였고, AITT는 높은 erythrocyte sedimentation rate 수치, 1분 및 5분 아프가 점수와 연관성을 보였다. 결론: 루푸스 산모의 산전관리에서 임상 증상과 혈액학적 면역학적 검사를 관찰함으로써 조기분만, 유산, 사산을 줄이고, 신생아에서 나타날 수 있는 합병증을 줄여나가야 한다. 기존의 C3/C4보체, 항dsDNA 검사에 AITT를 추가함으로써 루푸스 산모의 산전관리에 도움을 받을 수 있을 것으로 생각된다. Objective: The purpose of this study was to evaluate the correlation between complement (C3 and C4) levels, anti-dsDNA titers, autoimmune target test (AITT), and pregnancy complications. Methods: Ninety-three pregnancies with systemic lupus erythematosus (SLE) were analyzed retrospectively. We evaluated the course of the pregnancy and outcome, pregnancy complications, progression of SLE, maternal complications of SLE, drugs taken before and after pregnancy, neonatal outcomes, and C3/C4 levels, anti-dsDNA titers, and AITT results. SPSS ver. 14.0 was used for statistical analysis. The average and standard deviation are shown. Chi-square test and Pearson correlation test were used for statistical analysis. The significance level was set at 0.05. Results: The C3 level was associated with maternal leukopenia, elevated serum C-reactive protein (CRP) elevation, hematuria, hypertension, and preterm premature rupture of membranes. The C4 level was associated with maternal proteinuria, hematuria, hematologic disease, and admission to the neonatal intensive care unit. The anti-dsDNA titer was associated with elevated maternal serum CRP, oligohydramnios, and neonatal anti-Sj?gren``s syndrome B (La) antibody. The AITT result was related to elevated erythrocyte sedimentation rate, and 1 and 5 minute Apgar scores. Conclusion: Our result show that adding the AITT to conventional C3/C4 and anti-dsDNA testing in gravidas with SLE might help antenatal care.

통신비밀보호와 국민의 알권리 指定討論 要旨(4)

黃 鎬 澤 한국법학원 2005 저스티스 Vol.- No.88

Research on the impact of margin trading and investor sentiment on the volatility of the Science and Technology Innovation Board—based on the VAR model

심건군,황 명 호 사회혁신기업연구원 2023 혁신기업연구 Vol.8 No.4

With the launch of the Science and Technology Innovation Board, the impact of margin trading and investor sentiment on the market has become increasingly prominent. Therefore, exploring the relationship and impact between them is of great significance for preventing market risks. This article selects daily data from December 31, 2019, to November 6, 2023. The dependent variable is the Shanghai Science and Technology Innovation 50 Index (STAR50). The influencing factors are the financing balance of the Science and Technology Innovation Board (F), the securities lending balance (SL), and investor sentiment (IS). The VAR method is used to study the dynamic impact path of F, SL, and IS on STAR50. The results show that there is a long-term equilibrium relationship between F, SL, IS, and STAR50. From the perspective of the impulse response, STAR50 is mainly affected by securities lending, followed by financing, and finally investor sentiment. This shows that securities lending is one of the most important factors among the factors affecting the Science and Technology Innovation Board stock index. It can be seen through variance decomposition that the explanation ratios of F, SL, and IS for the fluctuations of STAR50 were stable at 0.66%, 2.37%, and 0.51%.This indicates that margin trading has to some extent affected the volatility of the Science and Technology Innovation Board. Therefore, our research findings contribute to a better understanding of the operational mechanism of the Science and Technology Innovation Board market and provide reference for preventing market risks.

늑골-광배근 복합조직이식을 이용한 두부 및 하악재건 치험례

국광식,황 호,허준평,이창주 大韓成形外科學會 1995 Archives of Plastic Surgery Vol.22 No.4

One stage composite tissue transfer by using microsurgery is one of the challenging event in reconstructive surgeons. Recently we experienced one case of extensive mandible defect which included overlying soft tissue and other case of scalp defect such as cranial bones. This paper includes successful 6 cases of osteomyocutaneous flap(ribs and latissimus dorsimuscle) by using microsurgical technique.