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황유경,김형아,전병학,이세훈,안규동,김도희,허용,이승혜 한국보건교육건강증진학회 2007 한국보건교육·건강증진학회 학술대회 발표논문집 Vol.- No.-
본 연구는 2004년 9월부터 2007년 2월까지 서울, 경기 일부지역의 다중이용시설(노인시설, 대규모 점포, 대학병원, 어린이집, 지하역사, 터미널)을 대상으로 실내ㆍ외 공기 중 미세먼지와 내독소에 관하여 조사하였다. 총 30개 시설을 대상으로 한 미세먼지의 측정결과 I/O Ratio가 1.23, PM2.5의 경우는 0.60으로 미세먼지 농도는 실외보다 실내에서 높았다. 미세먼지 중 내독소의 I/O는 1.26이었으며 시설군 간 유의한 차이가 있었다(p=0.07). PM₂.?중 .내독소 농도역시 I/O는 3.72로 실외보다 실내에서 내독소의 농도가 높았고 시설군 간 유의한 차이가 있었다(p=0.02). 각 시설군의 미세먼지와 내독소 간의 상관관계는 유의하지 않았다(P>0.05)
배(梨)의 메탄올 추출물이 마우스 복강대식세포의 nitric oxide와 tumor necrosis factor-a 생성에 미치는 영향
황유경,표명윤 숙명여자대학교 약학연구소 2004 약학논문집-숙명여자대학교 Vol.21 No.-
This study was conducted to investigate the effects of pear methanol extracts (P-M) on mouse peritoneal macrophages. Peritoneal macrophages were isolated from mice and cultured with or without LPS in the presence of P-M (0.16, 0.31, 0.63, 1.25, 2.50mg/ml) for 48hrs (TNF-a) or 96hrs (NO). NO levels in culture supernatant were determined by measuring the amount of nitrite, the stable oxidation product of NO, and TNF-a levels in culture supernatant were assayed by a sandwich ELISA. P-M significantly stimulated NO and TNF-a production from peritoneal macrophages at all doses. However, LPS-induced NO production was inhibited by P-M in a dose dependent manner and LPS-induced TNF-a production was not altered by P-M.
황유경,김형일,김남경,박정민,정홍석,Hwang, Yu Kyeong,Kim, Hyung-Il,Kim, Nam Kyung,Park, Jung Min,Cheong, Hong Seok 대한면역학회 2002 Immune Network Vol.2 No.1
Background: Viral antigens presented on the cell surface in association with MHC class I molecules are recognized by CD8+ T cells. MHC restricted peptides are important in eliciting cellular immune responses. As peptide antigens have a weak immunigenicity, pH-sensitive liposomes were used for peptide delivery to induce effective cytotoxic T lymphocyte (CTL) responses. In the previous study, as the HBx peptides could induce specific CTLs in vitro, we tested whether the HLA-A2/$K^b$ transgenic mice that were immunized by HBx-derived peptides could be protected from a viral challenge. Methods: HBx-peptides encapsulated by pH-sensitive liposomes were prepared. $A2K^b$ transgenic mice were immunized i.m. on days one and seven with the indicated concentrations of liposome-encapsulated peptides. Three weeks later, mice were infected with $1{\times}10^7pfu$/head of recombinant vaccinia virus (rVV)-HBx via i.p. administration. The ovaries were extracted from the mice, and the presence of rVV-HBx in the ovaries was analyzed using human TK-143B cells. IFN-${\gamma}$ secretion by these cells was directly assessed using a peptide-pulsed target cell stimulation assay with either peptide-pulsed antigen presenting cells (APCs), concanavalin A ($2{\mu}g/ml$), or a vehicle. To generate peptide-specific CTLs, splenocytes obtained from the immunized mice were stimulated with $20{\mu}g/ml$ of each peptide and restimulated with peptide-pulsed APC four times. The cytotoxic activity of the CTLs was assessed by standard $^{51}Cr$-release assay and intracellular IFN-${\gamma}$ assay. Results: Immunization of these peptides as a mixture in pH-sensitive liposomes to transgenic mice induced a good protective effect from a viral challenge by inducing the peptide-specific CD8+ T cells. Mice immunized with $50{\mu}g/head$ were much better protected against viral challenge compared to those immunized with $5{\mu}g$/head, whereas the mice immunized with empty liposomes were not protected at all. After in vitro CTL culture by peptide stimulation, however, specific cytotoxicity was much higher in the CTLs from mice immunized with $5{\mu}g/head$ than $50{\mu}g/head$ group. Increase of the number of cells that intracellular IFN-${\gamma}$ secreting cell among CD8+ T cells showed similar result. Conclusion: Mice immunized with XEPs within pH-sensitive liposome were protected against viral challenge. The protective effect depended on the amount of antigen used during immunization. XEP-3-specific CTLs could be induced by peptide stimulation in vitro from splenocytes obtained from immunized mice. The cytotoxic effect of CTLs was measured by $^{51}Cr$-release assay and the percentage of accumulated intracellular IFN-${\gamma}$ secreting cells after in vitro restimulation was measured by flow cytometric analysis. The result of $^{51}Cr$-release cytotoxicity test was well correlated with that of the flow cytometric analysis. Viral protection was effective in immunized group of $50{\mu}g/head$, while in the in vitro restimulation, it showed more spectific response in $5{\mu}g$/head group.