Fungal Community Associated with Genetically Modified Poplar During Metal Phytoremediation

허문석,임영운,유재정,전세욱,최영임,윤석환,박상철,김동일,이하나 한국미생물학회 2012 The journal of microbiology Vol.50 No.6

Due to the increasing demand for phytoremediation, many transgenic poplars have been developed to enhance the bioremediation of heavy metals. However, structural changes to indigenous fungal communities by genetically modified organisms (GMO) presents a major ecological issue, due to the important role of fungi for plant growth in natural environments. To evaluate the effect of GM plant use on environmental fungal soil communities, extensive sequencing-based community analysis was conducted, while controlling the influence of plant clonality, plant age, soil condition, and harvesting season. The rhizosphere soils of GM and wild type (WT) poplars at a range of growth stages were sampled together with unplanted, contaminated soil, and the fungal community structures were investigated by pyrosequencing the D1/D2 region of the 28S rRNA gene. The results show that the overall structure of the rhizosphere fungal community was not significantly influenced by GM poplars. However, the presence of GM specific taxa, and faster rate of community change during poplar growth, appeared to be characteristic of the GM plant-induced effects on soil-born fungal communities. The results of this study provide additional information about the potential effects of GM poplar trees aged 1.5–3 years, on the soil fungal community.

機能性 染料1, 빛변색성염료1, 3, 3-trimethylindolinobenzopyran 誘導體의 合成과 特性에 대한 硏究

琴三錄,李基奉,許文碩 高麗大學校 産業開發硏究所 1989 産業開發硏究 Vol.1 No.-

The photochromic dye, 1,3,3-trimethylindolinoenbenzopyran, were prepared from the reaction of 1,3,3-trimethy1-2-methyleneindoline with substituted salicylaldehydes. The spiro-ring formation of several spiropyran derivatives from their "open-form" precursors has been studied from spectrophotometric method in various solvents at room temperature. Rates(k₂)for the ring-closure reaction of spiropyrans were consistent with the first order kinetics. Plotting ?? versus solvent polarity parameter, ??, give a good linearity and λmax of spiropyrans versus solvent porarity parameter also showed a reasonably good linearity. A linear relationship (Y=4.5 X+1.6) was obtained between ?? and ∑σ, with ∑σbeing Taft and Brown substituent parameters.

PCR을 이용한 국내에서 안전성이 확인된 유전자재조합 옥수수의 분석 방법

허문석(Mun-Seok Heo),김재환(Jae-Hwan Kim),박선희(Sun-Hee Park),우건조(Geon-Jo Woo),김해영(Hae-Yeong Kim) 한국식품과학회 2003 한국식품과학회지 Vol.35 No.6

토양으로부터 메틸아민분해능을 지닌 미생물 분리 및 특성 분석

김민지,허문석,정유정,박수제 한국미생물학회 2020 미생물학회지 Vol.56 No.2

In this study, we investigated microbial isolation and characterization of methylamine degradation. Total 29 strains were isolated from various contaminated soil including landfill and identified as unrecorded species (n = 26) and candidate novel species (n = 3). In addition, these strains were classified into 19 genera based on the 16S rRNA gene sequence analysis. In particular, strain NJ1024 closely related to the genus Stenotrophomonas had a relatively high activity for methylamine degradation among other strains isolated in this study. Also, we assumed strain NJ1024 might have more potential versatile metabolisms for various pollutions. Finally, this study might provide a basic result for environmental benefit (e.g. odor removing) using indigenous microorganisms. 본 연구에서는 메틸아민분해능을 지닌 미생물의 분리 배양및 특성을 분석하고자 하였다. 이를 위하여, 국내의 다양한 오염토양(예, 쓰레기 매립지)으로부터 29주의 미생물들을 분리하였으며, 16S rRNA 유전자 염기서열 분석을 통하여, 17속에속하는 국내 미기록종 26종의 균주와 신종후보종 3종의 균주를 각각 확인하였다. 배양된 이들 미생물 중 Stenotrophomonas 속에 속하는 NJ1024 균주의 경우 비교적 높은 메틸아민 분해능을 지닌 것을 확인하였으며, 메틸아민 이외의 다양한 물질에 대한 분해기작 또한 지니고 있을 것으로 판단하였다. 본 연구결과는 국내 자생 미생물을 활용하여, 쓰레기 매립지 혹은축사인근의 오염토양 내의 악취발생 물질 제거에 응용될 수있는 기초적 정보를 제공할 수 있을 것이라 기대된다.

Photobacterium phosphoreum을 고정화하기 위한 Carboxymethylcellulose Sodium Salt [CMC] 담체의 이용성 연구

이용제,정성제,허문석,전억한 한국생물공학회 2000 KSBB Journal Vol.15 No.1

1.0%(w/v)의 CMC 담체는 고정화를 유지할 수 있는 점도를 가지며 0.1 M 이하의 양이온과 이온가교결합을 할 수 있는 농도이다. Luminometer tube내의 시료에 유동을 최소화함으로써 산소의 공급을 일정하게 하여 P. phosphoreum을 고정화 30분 후 bioluminescence intensity가 안정되어 바로 측정할 수 있는 장점을 가지고 있었다. 1.0%(w/v) CMC담체는 pH 6.92로 최적조건인 pH 7.0에 근접했으며, 발광기작에 필요한 산소전달(oxygen transfer)이 1.5%(w/v)~3.0%(w/v) CMC 담체보다 뛰어나 Bioluminescence intensity의 안정성을 부여하였다. Cr-화합물인 $Na_{2}CrO_{4}$, $K_{2}CrO_{4}$, $CrO_{3}$, CrK$(SO_4)_{2}$ 및 $CrCl_{3}$의 CMC담체에 대한 민감도는 $\gamma$값을 이용해서 $EC_{50}$값으로 나타내었을 때 $Na_{2}CrO_{4}$, $K_{2}CrO_{4}$, CrK$(SO_4)_{2}$ 및 $CrCl_{3}$ 의 $EC_{50}$값이 1.0%(w/v)CMC에서는 5.4~16.3 g/L으로 1.5%(w/v)~3.0%(w/v) CMC에서는 6.2~555.9 g/L의 범위로 나타났다. 이것은 1.0%(w/v) CMC가 낮은 독성 농도에서 bioluminescence intensity가 50% 감소함을 알 수 있고, 상관계수($R^2$)가 0.911~0.990 으로 높게 산출되었다. 따라서 1.0%(w/v) CMC 담체가 P. phosphoreum의 biolumincsene에 안정성을 주었으며, 독성물질에 가장 민감하다는 것을 알 수 있었다. Bioluminescence of Photobacterium phosphoreum has been used for the detection of pollutants in the environment. Immobilization method was used to maintain the stability of bioluminescence of P. phosphoreum. The carboxymethylcellulose was investigated to find out whether it was suitable for the immobilization of P. phosphoreum as a matrix without disturbing the bioluminescence emission. A maintenance of bioluminescence was determined from the P. phosphoreum immobilized on the various concentrations of carboxymethylcellulose. A relatively high bioluminescence intensity was shown with immobilized cells on 1%(w/v) carboxymethylcellulose. The effect of carboxymethylcellulose concentrations on the sensitivity of Crcompounds including $Na_{2}CrO_{4}$, $K_{2}CrO_{4}$, $CrO_{3}$, CrK$(SO_4)_{2}$ and $CrCl_{3}$ to the bioluminescence intensity. The calculated $EC_{50}$ showed that the linear relations between such substances and bioluminesence intensity were established.

자동식 및 수동식 햄머를 이용한 표준관입시험에 대한 연구

정성교,정경환,배종견,허문석 대한토목학회 1998 대한토목학회 학술발표회 논문집 Vol.1998 No.2

Diversity analysis of Saccharomyces cerevisiae isolated from natural sources by multilocus sequence typing (MLST)

엄유정,손수영,정동현,허문석,김창무,박선영,신우창,이상진,어중혁,김계원,박천석 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.4

We used multilocus sequence typing (MLST) to analyze the diversity of natural isolates of Saccharomyces cerevisiae, the most important microorganism in alcoholic fermentation. Six loci, ADP1, RPN2, GLN4, ACC1, MET4, and NUP116, in S. cerevisiae genome were selected as MLST markers. To investigate genetic diversity within S. cerevisiae, 42 S. cerevisiae isolated from natural sources in Korea as well as six S. cerevisiae obtained from Genbank and four industrial S. cerevisiae were examined using MLST. Twenty-six polymorphic sites were found in the six loci. Among them, ACC1 had the most genetic variation with eight polymorphic sites. MLST differentiated the 52 strains into three clades. Alcohol fermentation results revealed that S. cerevisiae in Clade III produced less alcohol than those in Clades I and II. These results suggested that MLST is a powerful tool to differentiate S. cerevisiae and can potentially be used to select S. cerevisiae suitable for industrial use.

독성물질 농도에 따른 Photobacterium phosphoreum의 bioluminescence 변화

정계훈,정성제,이용제,허문석,전억한 한국산업미생물학회 2000 한국미생물·생명공학회지 Vol.28 No.1

본 연규에서는 발광미생물인 P. phosphoreum을 이용하여 수질 측정용 바이오센서를 개발하고자 free cell과 고정화 세포의 독성물질에 대한 반응성을 조사하였다. 고정화 물질로는 절차가 비교적 간단한 sodium alginate를 사용하였다. Alginate는 발광미생물의 빛 발생 대사를 저해하지 않을 뿐만 아니라 bioluminescence의 투과성이 탁월하였다. 독성물질의 MIC값을 보면 As_2O_3가 400 ppm, phenol이 800 ppm, SeO_2와 CrO_3가 60 ppm으로 나타났다, 독성물질 As_2O_3, phenol, SeO_2, CrO_3를 3가지의 조건 free cell과 Sr-alginate 및 Ca-alginate에 고정화한 세포에 투여하여 반응성을 조사하였다. Free cell이 가장 민감한 반응을 보였으며 free cell과 고정화 세포 모두 독성물질에 대하여 발광량이 농도에 비례하여 감소하였다. 특히, 고정화 세포인 경우 free cell보다 독성물질의 mass transfer에 미치는 영향때문에 민감성은 떨어지지만 직선의 관계식을 나타내었다. Sr-alginate고정화 세포와 Ca-alginate고정화 세포의 독성물질에 대한 반응 양상은 비슷하게 나타났다. 독성물질에 대한 반응분석은 Gamma value로부터 EC_50을 구하여 각 독성물질의 농도와 P. phosphoreum의 발광량과의 상관관계 및 각 독성물질의 독성정도를 산출하였다. Photobacterium phoshoreum was used for the study of bioluminescence response to toxic substances including phenol, As_2O_3, SeO_2, and CrO_3 in view of developing monitoring system. Measurement of inhibition of bioluminescence in P. phosphoreum has been proposed as a sensitive and rapid procedure to monitor toxic substances. The concentration of toxic substance causing 50% light reduction(EC_50) in bioluminescence intensity was determined with free and immobilized P. phosphoreum. The minimum inhibitory concentration(MICs) for bioluminescence emission were found to be 400 ppm for As_2O_3, 800 ppm for phenol, 60 ppm for SeO_2 and 60 ppm for CrO_3, respectively. The linear correlation between Gamma value and the concentration of toxic substances was obtained and EC_50 was calculated from the linear correlation. The free cells were shown to be more sensitive to toxic substances than cells immobilized on Sr-alginate and Ca-alginate, However, the linear regrassion curves were derived form the Sr-alginate immobilized cells indication the immobilization method is a useful tool for monitoring of toxic substances under the more stable condition of bioluminescence.

[論文] 眞圓度 測定器의 誤差特性에 대한 解析-위상차법-

한응교(E. K. Han),허문석(M. S. Her),박익근(I. G. Park) 한국자동차공학회 1988 오토저널 Vol.10 No.3

A phase different method to evaluate the instrument error of roundness measuring instru- ment and the form error of specimens for the calibration of the instrument is used. An instru-<br/> ment with a rotary table supported by an air bearing was calibrated by using the standard balIs as a standard. The calibration was carried out repeatedly by setting the same ball in 12 phase angles(per 30 °) on the table and by recording their roundness errors with a magnification of 100,000 times. As a result of data analysis of all the observations, readout at each of 144 orien- tations(per 2.5 °) from recorded data file, the error of performance of the instrument and the specimens are separated.<br/> In the particular instrument used in the present experiment, the error of the instrument was determined with the accuracy of 0.0164 (μm) and the form error of the secimens was determined with the accuracy of0.0264,0.0172 (μm), respectively.<br/> If the instrument was calibrated by using the above specimens, then the accuracy of the measurement of roundness error can be improved to about 0.017 (μm).

겹침확장 유한요소법을 이용한 강재와 준취성 재료의 균열진전해석

이상호(Lee Sang-Ho),허문석(Huh Moon-Seok),김명원(Kim Myoung-Won) 대한토목학회 2004 대한토목학회 학술대회 Vol.2004 No.10