수소를 에너지원으로 이용하는 탈질 세균의 분리 및 생리학적 특성

한혜성,박두현 서경대학교 산업기술연구소 2002 産業技術硏究所論文集 Vol.12 No.-

There are some environments that are lack of oxygen or to which oxygen are not provided. Under anaerobic or anoxic environment, some of microorganisms have to produce free energy by anaerobic respiration such as denitrification, sulfate-reduction, carbonate reduction and metal reduction metabolism. Most of denitrificans are facultative anaerobic bacterium which uses nitrate instead of oxygen as a final electron acceptor. In this experiment, I isolated denitrificans to use hydrogen as a energy source and extracted a nitrate reductase from bacteria cells, so, I certified a physiological characterization of denitroficans and enzyme activity of nitrate reductase through measurement of bacterial growth rate and denitrofication efficiency. Facultative anaerobic bacterium exchange a metabolic pathway from an aerobic respiration to an anaerobic respiration in anaerobic condition. These characterized denitroficans showed the better growth rate in aerobic condition than in anaerobic condition. And I could certify it through denitrfication efficiency that these bacterium respire as dissociate nitrate in anaerobic condition to be lack of oxygen for energy synthesis to need metabolism. The enzyme activity of nitrate reductase to be isolated from bacterial cells used a method of Michaelis-Mentens. Km and Vmax of DN4 enzyme was 1.5mg/L and 0.14mg/L. Moreover, Km and Vmax of DN5 enzyme was 3mg/L and 0.19mg/L. Therefore an affinity about substrate of DN4 nitrate reductase is higher than DN5 nitrate reductase as twice.

이비즈니스 ; e-Learning에서의 객관주의와 구성주의 기반 교수-학습 모델에 관한 연구

한혜성,김병초 한국경영정보학회 2004 한국경영정보학회 학술대회논문집 Vol.2004 No.-

종설 복잡성 애도의 이해

한혜성,최원준,허승,허휴정,채정호 한국인지행동치료학회 2016 인지행동치료 Vol.16 No.3

가까운 사람과 사별한 개인은 애도라는 정상적인 반응을 보이며 애도는 일반적으로 시간이 지남에 따라 자연스럽게 경감된다. 그러나 개인에 따라 애도의 정도와 기간이 달라질 수 있으며 이에 DSM-5에서는 지속성 복합 애도장애를 추가 연구가 필요한 진단적 상태에 포함시켜 추후 이에 대한 연구가 지속될 수 있도록 하였다. 지속성 복합 애도장애는 지속적 갈망/ 그리움, 강렬한 슬픔과 정서적 고통, 죽은 사람에 대한 집착, 죽음을 둘러싼 상황에 대한 집착 중 한 개 이상이 성인의 경우 12개월 이상 지속되고 죽음에 대한 반응적인 고통 6가지와 사회적/정체성 붕괴 6가지의 총 12가지 증상 중 6가지 이상을 현저하게 경험할 때 진단할 수 있다. 복합애도 척도(Inventory of Complicated Grief: ICG)는 애도를 평가하기 위해 가장 자주 쓰이는 평가 척도 중의 하나로 타당도가 높다고 알려져 있다. 복잡성 애도는 정상애도, 우울장애, 외상후 스트레스 장애와 감별이 필요하다. 복합성 애도 치료(complicated grief treatment: CGT)는 무작위대조군 연구에서 개인정신치료에 비하여 치료 효과가 있으며 빠른 치료 반응을 보인다는 결과를 보였다. 본 종설은 복잡성 애도에 대해 소개하고 이해를 돕는 것을 목적으로 하고 있다. When an individual lose someone intimate such as a family member, a relative or a close friend, the bereaved person shows normal reaction of grief for the loss and these symptoms of grief generally wane out with time as a natural process. In practice, however, grief shows a large difference in severity and duration depending on the individual and situations of the loss. Therefore diagnosis of complicated grief has been discussed, when the bereaved person experiences intense grief even after a certain period of time and the disturbance causes significant distress or impairment in social, occupational, or other important areas of functioning. DSM-5 includes persistent complex bereavement disorder in proposed criteria and provides a basis for possible placement in forthcoming editions of DSM. Symptoms of persistent complex bereavement disorder are persistent yearning/longing for the deceased, intense sorrow, preoccupation with the deceased and the circumstances of the death, reactive distress to the death and social/identity disruption. The bereaved individuals may also desire to die because they hope to be with the deceased. There are several scales to measure grief and symptoms of complicated grief. Inventory of complicated grief (ICG) is one of the most often used scales and the validity of ICG is relatively high. The complicated grief, it is necessary to distinguish normal grief and other psychiatric disorders such as depressive disorder, post-traumatic stress disorder. Complicated grief treatment (CGT) consists of 10 modules would be a treatment for complicated grief. A randomized control trial study comparing the efficacy of CGT with interpersonal psychotherapy shows higher response rates and faster time to response. This review would help to introduce complicated grief into the clinical field in psychiatry.

보험산업에서 e-Learning의 혁신성 -확산(diffusion)과 동화(assimilation)-

한혜성,고영기,권태형 한국경영정보학회 2003 한국경영정보학회 학술대회논문집 Vol.2003 No.-

영어 그림책을 활용한 5세 누리과정 연계 동화활동 계획안 개발

한혜성,이종희 한국유아교육학회 2019 정기학술대회 논문집 Vol.2019 No.1

Combination of non-ablative fractional laser with Q-switched laser for the treatment of Becker’s nevus: efficacy and limitations

한혜성,홍준기,유광호,서성준 대한피부과학회 2023 Annals of Dermatology Vol.35 No.1

Becker’s nevus (BN) is a benign hamartoma that may present as a distressing cosmetic problem. The treatment of BN poses a significant challenge as current therapeutic modalities aresuboptimal and have an increased risk of adverse effects, such as scarring and dyspigmentation. We present the use of non-ablative fractional laser therapy combined with Q-switchedNd:YAG laser as a possible therapeutic option for BN treatment and review relevant literatureto discuss its efficacy and limitations.

A randomized, prospective, split-face pilot study to evaluate the safety and efficacy of 532-nm and 1064-nm picosecond-domain neodymium:yttrium-aluminum-garnet lasers using a diffractive optical element for non-ablative skin rejuvenation: clinical and hi

한혜성,홍준기,박수정,박병철,박귀영 대한피부과학회 2023 Annals of Dermatology Vol.35 No.1

Background: The advent of fractionated picosecond (ps) lasers has provided an opportunityto explore new ways of creating microinjuries in the skin to induce skin rejuvenation. Objective: To compare the efficacy and safety of diffractive optical element (DOE)-assistedps neodymium: yttrium-aluminum-garnet (Nd:YAG) lasers with 532-nm and 1,064-nmwavelengths (532-nm and 1,064-nm Nd:YAG P-DOE) using a novel fractional handpiece forthe treatment of photoaged skin. Methods: An ex vivo guinea pig skin experiment was performed by evaluating the histologyof the skin after 532-nm Nd:YAG P-DOE irradiation. A randomized, prospective, split-facestudy was performed on eight subjects with 532-nm and 1,064-nm Nd:YAG P-DOE. Results: Based on the histological evaluation using ex vivo guinea pig skin, a reasonablesafety profile and the potential to generate effective skin rejuvenation was observed usingthe 532-nm Nd:YAG P-DOE. Results demonstrated that both 532- and 1,064-nm Nd:YAGP-DOE were similarly effective in improving skin texture and skin pores; however, 532-nmNd:YAG P-DOE was more effective in treating dyspigmentation. Conclusion: At a preliminary level, this study revealed that 532-nm and 1,064-nm ps Nd:YAGlasers using DOE fractional technology may improve photoaged skin. In conclusion, 532-nmNd:YAG P-DOE may be especially beneficial for skin with epidermal pigmentary lesions.

Posttraumatic Symptoms and Change of Complicated Grief among Bereaved Families of the Sewol Ferry Disaster: One Year Follow-up Study

한혜성,윤지애,허혜정,허승,황지현,주지영,윤영애,신은경,최원준,이상민,채정호 대한의학회 2019 Journal of Korean medical science Vol.34 No.28

Background: The objective of this study was to determine whether severity and severity change of coexisting psychiatric symptoms might affect change of complicated grief (CG) regarding the Sewol ferry disaster. Methods: Data from a cross-sectional survey were obtained 18 months (Time 1) and 30 months (Time 2) after the disaster. We ascertained sociodemographic variables and variables obtained from self-reporting questionnaires (i.e., CG, depression, anxiety, post-traumatic stress disorder [PTSD], insomnia, embitterment, and suicidal risk) among 56 bereaved family members. Results: Severity of other psychiatric symptoms at Time 1 had no effect on change of CG at Time 2. However, changes in severity of PTSD over a year affected change of CG. Conclusion: It is important to evaluate changes in severity of PTSD and its treatment during management of CG, especially when it involves bereaved families experiencing a traumatic accident.

Innovative Use of Negative Air Ions As an Alternative Therapy for Acne Vulgaris: A Report of Three Cases

한혜성,정국진,이해웅,심주현,서성준,박귀영 대한피부과학회 2022 Annals of Dermatology Vol.34 No.3

Acne vulgaris is a universal skin disease with multifactorial pathogenesis. Although an ex- tensive range of treatment options exist for acne, a substantial number of patients are still struggling for an optimal treatment option due to the side effects or contraindications to the conventional acne treatment. Negative air ions (NAIs) are electrically charged molecules that naturally exist in the atmosphere. Since they are natural component of air, there are no known side effects and contraindications to their application. Furthermore, among the identified benefits of NAIs, certain mechanisms are related to acne pathogenesis, allowing them to be attractive candidates for acne treatment. Here, we describe three patients with acne who showed considerable clinical improvement after NAI therapy. All of the patients had failed to tolerate traditional acne treatment options. In all three cases, considerable im- provement was observed in acne severity and the number of total lesions. Based on the three cases and a review of literature underlying the effects of NAIs, we suggest that NAIs may be a safe and effective alternative therapeutic option for acne vulgaris.

우울장애 환자에서 장애의 심각도와 증상 평가 척도와의 상관관계

한혜성,전양환,한상익,경미하,박이진 대한우울조울병학회 2015 우울조울병 Vol.13 No.2

Background and Objectives : The aim of this study was to investigate the relationship between the severity of depressive disorder and the rating scales. Materials and Method : One hundred fourteen individuals with major depressive disorder or depressive disorder, not otherwise specified were recruited in the Department of Psychiatry, College of Medicine, Incheon St. Mary’s Hospital, The Catholic University of Korea. The participants completed examination, which included observer-rating scales and self-rating scales for depressive symptoms. We investigated correlation between the severity of depression and the symptom rating scales. Results : There was significant relationship between the scores of scales and severity of depression. There is stronger correlation between scores of observer-rating scales and severity of depression than between scores of self-rating scales and severity of depression. However, in the severe group, all scales show higher correlation between scores of scales and severity of depression. Conclusion : It tends to be more accurate using observer-rating scales to distinguish the severity of depression than other groups. However, in the severe major depression, both observer and self rating scales and the number of depressive symptoms are considered as tools to measure the severity of depression