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연구 - 유전자 전이방법을 이용한 효율적인 형질전환 닭 생산 기술 확립 - 인위적 계란 성분 조절이 가능한 새로운 형질의 닭 품종 대량 생산 체계 확립
한재용,박태섭,Han, Jae-Yong,Park, Tae-Seop 대한양계협회 2012 월간 양계 Vol.44 No.8
본고는 서울대학교 한재용 교수 연구팀(공동 연구자 박태섭 박사)이 닭에서 바이러스를 사용하지 않은 유전자 전이방법을 이용한 효율적인 형질전환 닭 생산 기술 확립에 성공하여 미국 학술원 회보(Proceedings of the National Academy of Sciences)에 게재함에 따라 그 성과를 기리고 독자들에게 알리기 위해 한재용 교수에 의뢰해 농가들이 알기쉽게 정리한 내용이다. 형질전환 닭은 인간의 질병 연구 및 새로운 치료제 개발을 위한 다양한 실험 모델 생산에 활용되어 양계산업에 다양하게 사용될 것으로 기대되고 있다.
재래돼지 Melanocortin Receptor 1(MCIR)의 변이에 관한 연구
한재용,정일정,김태헌,윤두학,연성흠,최봉환,노환국,김남순,이화진 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.3
The melanocortin 1 receptor (MC1R) gene is the major candidate gene for coat color in pig since it is assumed to be controlled by an allele at the Extension (E) locus. A 702 by fragments of the MC1R gene were amplified by PCR, cloned and sequenced from 6 heads of Korean native pig. Of them, 4 pigs possessed allele#2 which is associated with black coat color in pig, the others had allele#1 which revealed a unique allele in European wild boar reported by Kijas et al. (1998). PCR-RFLP was carried out to observe their alleles from 6 different pig breeds including Korean native pig, Berkshire, Duroc, Landrace, Large White, and Meishan pig. Two pairs of primers were designed for amplifying the MC1R gene fragment corresponding to five polymorphic sites. PCR products were digested 5 different restriction enzymes. Nsp I , MspA 1 I , Rca I , Hha I and Acc II , and electrophoresed in 2.5% Metaphor agarose gel. All breeds except for Korean native and Meishan breeds were the same alleles reported by Kijas et al. (1998). Of 9 Meishan pigs tested in this study, 4 heads possessed the same allele, 5 heads were different alleles reported by Kijas et al. (1998). A total of 15 different alleles revealed in Korean native pig. The results of this study strongly suggest that black coat color in Korean native breed was associated with a constitutively active receptor at codon 99 (L99P).
소 품종별 Melanocortin Receptor 1(MC1R) 유전자의 유전자형 빈도에 관한 연구
한재용,김경남,오성종,정일정,김태헌,윤두학,박응우,이혜영,탁태영 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.6
The melanocortin 1 receptor (MC1R) plays a central role in regulation of eumelanin(black/brown) and phaeomelanin (red / yellow) synthesis within the mammalian melanocyte and encoded by the classical Extension (E) coat color locus. The objectives of this study were carried out to observe the genotype frequencies of MC1R in 1,044 samples of imported beef and eight different cattle breeds including Hanwoo, Holstein, Angus, Brown-Swiss, Charolais, Limousin, Simmental and Hereford. The primers for the amplification of bovine MC1R gene were designed based on a bovine MC1R gene sequence (GenBank accession no. Y19103). A size of 350 by was amplified by polymerase chain reaction(PCR), digested with each or together of two different restriction enzyme, BsrFI and MspAII, and electrophoresed in 2.5% or 4% Metaphore agarose gel for determination of genotypes. One thousand and forty four samples including eight different cattle breeds and imported beef were determinated their genotypes by PCR-RFLP. Genotype frequencies of Hanwoo were 0.10 in E e and 0.90 in ee. Allele E^D was shown in all of Holstein and Angus breeds tested which have black coat color phenotypes. However, genotype frequencies of Limousin and Simmental breeds were 0.21 and 0.07 in E^DE^D, 0.00 and 0.03 in E^De^+ , 0.38 and 0.35 in E^De, respectively. These results of this study suggest that alleles of the bovine MC1R gene are associated strongly with coat color except for Simmental and Limousin breeds with dark brown, light yellow or mosaic coat color of red and brown. Therefore, it needs to be studied on relationship between A and E loci and coat color phenotypes. Furthermore, genotypes of the bovine MC1R gene can be used as a DNA marker for distinguishing. beef between Hanwoo and Holstein and Angus having black coat color phenotype.
한재용 한국금융연구원 2008 주간 금융 브리프 Vol.17 No.6
금년 들어 은행들은 정기예금이 빠른 속도로 증가함에 따라 지난해 극심했던 자금조달난이 해소되는 모습임. 그러나 주식시장이 호조를 보일 경우 머니무브 현상은 재현될 소지가 있음. 따라서 은행들은 재원조달수단을 다양화하는 한편 비이자수익원 확보에 대한 노력을 지속하는 것이 필요함.
Therapeutic Efficacy of Spironolactone for Central Serous Chorioretinopathy
한재용,김용준,최은영,이준원,이지환,김민,변석호,김성수,이승규 연세대학교의과대학 2022 Yonsei medical journal Vol.63 No.4
Purpose: To evaluate the therapeutic effects and safety of oral spironolactone (SPRL) in patients with central serous chorioretinopathy(CSC). Materials and Methods: The medical records and imaging data of patients diagnosed with CSC and treated with SPRL were retrospectivelyreviewed. Central macular thickness (CMT), subretinal fluid (SRF) height, subfoveal choroidal thickness (SFCT), andbest-corrected visual acuity (BCVA) at baseline, at 1, 3, and 6 months, and at the last visit after the treatment were analyzed. Results: In total, 103 patients with 107 eyes were included. The mean age of the patients was 51.5±9.3 years, and 77 (72.0%) weremale. The mean follow-up duration was 48.6±40.2 weeks. The mean duration of oral SPRL therapy was 15.5±13.4 weeks. CMT, SRFheight, and SFCT improved significantly at 1, 3, and 6 months after SPRL therapy and at the last follow-up. BCVA, however, showedno significant change at any time point. The rate of complete resolution of SRF at 1 month was higher in those with chronic CSCthan in those with acute CSC (21.1% vs. 6.0%, respectively). Recurrence occurred in 14 (13.1%) eyes after the complete resolution ofSRF. Older age (p=0.001), a greater number of previous intravitreal bevacizumab injections (p=0.006), and poor initial visual acuity(p=0.048) were associated with recurrence. No permanent adverse effects were observed. Conclusion: Oral SPRL showed therapeutic benefits in patients with CSC in terms of SRF resolution, but relatively frequent recurrencewas observed, especially in older patients.