Melatonin이 Cisplatin에 의한 신장 독성에 미치는 영향

최혜정 ( Choe Hye Jeong ),신영호 ( Sin Yeong Ho ),문교철 ( Mun Gyo Cheol ),송대규 ( Song Dae Gyu ),김인철 ( Kim In Cheol ),서상혁 ( Seo Sang Hyeog ),곽춘식 ( Gwag Chun Sig ),장은주 ( Jang Eun Ju ),김현철 ( Kim Hyeon Cheol ) 대한신장학회 2004 Kidney Research and Clinical Practice Vol.23 No.2

목 적 : Cisplantin은 항암제로 널리 사용되나 활성산소를 생성하여 신장 기능 손상 등의 부작용이 있는 것으로 알려져 있긴 강력한 항산화제인 melatonin은 cisplatin에 의한 산장독성을 줄일 수 있을 것이다. Cisplatin에 의한 신장 독성에 대하여 melatonin의 방어 효과를 알아보고자 하였다. 방 법 : 실험군을 대조군인 제 1군, cisplatin 투여군인 제 2군 및 cisplatin과 melatonin을 투여한 제 3군으로 나눈 후 신장 조직의 형태학적 변화와 더불어 산화적 손상에 대한 연구를 수행하였으며 과산화수소, malondialdehyde 및 항산화효소의 활성도를 측정하였다. 결 과 : 혈청 BUN (p<0.001), creatinine 농도 (p<0.001) 및 신장의 과산화수소 생성량은 제 1군에 비해 제 2군에서 증가하였고, 제 2군에 비해 제 3군에서는 감소되었으며, 활성산으로 인한 손상 표지자인 malondialdehyde 농도도 같은 경향을 나타내었다. Creatinine 청소는 제 1군에 비해 제 2군에서 감소를 보였고, 제 2군에 비해 제 3군에서는 증가되었다. 신장의 superoxide dismutase 활성도는 제 1군에서 제 2군에 비해 감소하였고 catalase 활성도는 제 1군에 비해 제 2군에서 증가되었으며, 제 1군에 비해 제 3군에서는 더욱 증가되었다. Glutathione peroxidase는 통계학적으로 의미 있는 변화가 없었다. 또한 광학 현미경 소견을 보면 제 2군의 사구체에서는 특이한 변화가 없었으나, 근위 곱슬 세관은 심한 괴사를 나타내었고, 제 3군의 근위 곱슬 세관에서 중등도의 괴사를 나타내었으며 제 2군보다 경한 변화를 보였다. 결 론 : Cisplatin은 신장에서 superoxide dismutase 활성도 증가와 catalase의 활성도를 저하시켜 과산화수노의 생성을 유도하여 신장 손상을 유발하는 것으로 생각되며, melatonin은 superoxide dismutase 활성도 증가와 catalase 활성도 증가를 유도하여 cisplatin에 의해 유발된 신장 손상을 줄이는 것으로 생각된다. Background : Cisplatin (CP), an antitumor agent widely used in the treatment of cancers, has nephrotoxicity. This side effect is closely related to oxidative stress. In the present study, we attempted to reduce CP-induced nephrotoxicity in rats by administering melatonin, an antioxidant. Methods : Male Sprague-Dawley rats were divided into different groups and were treated as follows : ⑴ saline control; ⑵ CP (16 mg/kg, i.p.); ⑶ CP plus melatonin (10 mg/kg, i.p.). The rats were sacrificed at the 6th day after CP treatment. To evaluate renal damage, BUN, serum creatinine, creatinine clearance and microscopic examination were done. Hydrogen peroxide which is one of the oxygen free radicals, and malondialdehyde which is known as a marker of the oxygen free radical mediated injury, and the activities of the antioxidant enzymes such as superoxied dismutase, catalase, and glutathione peroxidase were also measured. Results : CP-treated rats showed the increase of BUN, serum creatinine, malondialdehyde, hydrogen peroxide and superoxide dismutase (SOD) in kidney. And CP-treated rats also showed the decrease of creatinine clearance and catalase levels. CP-treated rats showed severe tubular necrosis in proximal convoluted tubules under the light microscopic examination. The light microscopic finding and all of the parameters except SOD were restored in the rats injected with CP plus melatonin than those with CP alone. SOD level was higher in the rats injected with CP plus melatonin than that with CP alone. Conclusion : These results suggest that melatonin suppresses CP-induced nephrotoxicity by suppressing the production of reactive oxygen species via the activation of SOD and catalase. (Korean J Nephrol 2004;23(2):205-212)

중,고등학생의 과학과 자기주도학습에 대한 관심수준 및 실행수준 분석

최혜정 ( Hye Jeong Choe ),정진수 ( Jin Su Jeong ),김상호 ( Sang Ho Kim ) 경북대학교 과학교육연구소 2015 科學敎育硏究誌 Vol.39 No.1

본 연구의 목적은 과학과 자기주도학습에 대한학생의 관심수준 및 실행수준을 관심에 기초한 교육과정 실행 모형(Concerns-Based AdoptionModel: CBAM)을 바탕으로 분석하고 이들 간의 상관성을 조사하여 학생 개인의 관심수준에 맞는 자료를 제공하고 보다 높은 실행수준으로 향상될 수 있도록 지원책을 제시하고자하는 데 있다. 선행 연구들을 분석하여 학생의 과학과 자기주도 학습에 대한 관심수준과 실행수준을 분석하기 위한 분석틀을 개발하고 이를 바탕으로 측정 도구를 개발하였다. 중.고등학생을 대상으로 설문조사한 결과를 통계적으로 분석하고 그 결과를 집단(지역, 학년, 성별)별로 나누어 비교 분석하였다. 과학과 자기주도학습에 대한 학생의 관심수준은단계0 무관심이 가장 많았고, 실행수준은 수준Ⅱ준비 단계가 가장 많았다. 집단 간의 비교에서는 관심수준은 성별에서 통계적으로 여학생이 남학생보다 높은 관심수준을 보였고, 실행수준은 중학생보다 고등학생이 높은 실행수준을 보이고 남학생보다 여학생이 높은 실행수준을 보였다. 마지막으로 관심수준과 실행수준의 상관성 분석에서 통계적으로 유의한 차이를 보였다. The purpose of this study was to measure middle and high school students`` stage of concern(SoC) and their level of use(LoU) on the self-directed learning in science learning based on the CBAM(Concern-Based Adoption Model). Additionally, this research was designed to analyze the difference between the degree of students`` SoC and their LoU according to the their background variables. For this, 440 middle and high school students participated in the research. The results of this study were as follow: Firstly, since the students`` SoC and LoU about the self-directed learning in science learning are low(Stage0 : awareness and LevelⅡ: preparation), we should draw students`` immediate concern by developing training programs that would enable them to actually participate in the process of implementing the self-directed learning. Secondly, the students`` SoC and LoU on self-directed learning in science learning vary depending on their residence, gender, and grade. This is the reason why we have to develop customized training programs on self-directed learning that suits their background. Thirdly, it shows that students, who have higher concern on self-directed learning in science learning, implement it better than those who are not concerned with it at all. It implies that we need a training program that considers both the students`` concern and implementation on self-directed learning in science learning.

말기 신부전 환자의 중성구에서 활성 산소 대사

최혜정 ( Choe Hye Jeong ),권진경 ( Kwon Jin Gyeong ),문교철 ( Mun Gyo Cheol ),장은주 ( Jang Eun Ju ),곽춘식 ( Gwag Chun Sig ),한승엽 ( Han Seung Yeob ),황은아 ( Hwang Eun A ),박성배 ( Park Seong Bae ),김현철 ( Kim Hyeon Cheol ) 대한신장학회 2004 Kidney Research and Clinical Practice Vol.23 No.3

목적 : 말기 신부전 환자에서는 감염에 대항하는 중성구의 기능장애 기전을 밝히고자 하였다. 방법 : 혈액투석을 하는 말기 신부전 환자 50명과 정상인 40명에서 중성구를 분리한 후 superoxide 음이온, 과산화수소의 농도 및 malondialdehyde 농도, 항산화 효소인 superoxide dismutase (SOD), catalase, glutathione peroxidase 및 myeloperoxidase의 농도를 측정하였으며, SOD의 조효소인 구리와 아연 이온의 농도를 측정하고, SOD에 대한 역전사 중합효소 연쇄 반응을 실시하였다. 결과 : 말기 신부전 환자의 중성구에서는 정상인에 비해 superoxide 음이온, catalase, glutathione peroxidase, malondialdehyde가 증가하였으며, SOD, 과산화 수소의 양, 아연 이온의 농도는 감소하였다. 또한 말기 신부전 환자에서는 SOD의 유전자 발현이 억제되었으며 glutathione peroxidase의 활성은 유의한 변동이 없었다. 결론 : 말기 신부전 환자의 중성구에서는 유전자 발현 억제 및 조효소의 양적 감소에 의해 SOD의 활성이 저하되며, 이로 인한 superoxide 음이온의 증가는 중성구의 산화적 손상을 유발하는 것으로 생각된다. 또한 SOD의 활성 저하와 catalase의 활성 증가는 과산화 수소를 감소시켜 myeloperoxidase에 의해 전환되는 차아염소산의 감소를 초래함으로서 중성구 기능 장애 원인 중의 일부로 작용할 수 있음을 시사한다. Background: The present study was aimed to know the cause of impaired bactericidal activity, especially the metabolism of oxygen free radicals in neutrophils from patients with end-stage renal disease (ESRD). Methods : We measured the amount of superoxide anion, the activity of three antioxidant enzymes, myeloperoxidase, copper ion level, zinc ion level and the amount of malondialdehyde in neutrophils from patients with ESRD before and after hemodialysis. Reverse transcription-polymerase chain reaction (RT-PCR, for superoxide dismutase (SOD) was also done. Results : The malondialdehyde level, the amount of superoxide anion, catalase, and myeloperoxidase levels in the neutrophils from the patients with ESRD were higher than those from healthy controls. SOD activity, hydrogen peroxide level and zinc level were lower in ESRD patients. On the RT-PCR, the relative index, which is defined the ratio of the band densities for SOD to glyceraldehyde 3-phosphate dehydrogenase, was decreased in neutrophils from patients with ESRD. Glutathione peroxidase activity in the neutrophils from ESRD patients did not show any significant change. Conclusion : These results indicate that there are some alterations in metabolism of oxygen free radicals including lower levels of hydrogen peroxide which exerting a direct germicidal ability, due to decreased gene expression and mineral levels. And these alterations might be one of the major mechanisms of impaired microbicidal activity in patients with ESRD. (Korean J Nephrol 2004;23(3):429-438)

손자녀 양육 여부에 따른 조모의 주관적 건강상태, 우울, 삶의 질 및 자녀와의 갈등 비교

최혜정 ( Choe Hye-jeong ) 부모자녀건강학회 2017 부모자녀건강학회지 Vol.20 No.2

Purpose: This research compared the degrees of grandmothers’ subjective health status, depression, quality of life and conflict with their children between the grandmothers who take full charge of raising their grandchildren and the grandmothers who don’t and was being conducted to provide the basic data of arbitration for health improvement of grandmothers raising grandchildren. Methods: The study participants was 30 grandmothers who take full charge of raising the grandchildren and 30 grandmothers who don’t of grandchildren. Grandmothers’ subjective health status, depression, quality of life and conflict with their children between the grandmothers was measured by measurement tools developed by previous research. Data collecting were measured by self-report questionnaire. The data were analyzed using descriptive statistics, t-test, Pearson correlation coefficient by SPSS 23.0. Results: The depression score of grandmothers raising grandchildren was 26.60±12.04, which was significantly higher than that of 19.57±7.04 grandmothers were not raising grandchildren (t=2.76, p=.008). Also the conflict score of between children and grandmothers raising grandchildren was 30.70±10.72, which was significantly higher than that of 22.43±6.40 between children and grandmothers were not raising grandchildren (t=3.63, p=.001). Conclusion: The results showed that a solution to intervene the depression and conflict with their children of the grandmother who raise their grandchildren is needed.

백반증과 동반되어 퇴행을 보인 선천성 멜라닌세포성 모반

최혜정 ( Choe Hye Jeong ),구자경 ( Gu Ja Gyeong ),박영민 ( Park Yeong Min ),김형옥 ( Kim Hyeong Og ) 대한피부과학회 2004 대한피부과학회지 Vol.42 No.1

Congenital melanocytic nevi rarely develop depigmentation, and only a few cases have been reported in the literature. We, herein, present a case of congent\ital melanocytic nevus which has undergone progressive involution in association with vitiligo. A 23-year-old man had a dark brownish patch partially surrounded by the depigmented patch on his mid back. He also had multiple depigmented patches on his trunk and neck, which had developed since the age of 5. Histopathologic examination from the dark brownish patch adjacent to the depigmented area showed typical findings of compound nevus with a mild perivascular lymphocytic infiltration. TUNEL staining from the same lesion demonstrated many strong positive epidermal basal cells as well as dermal nevus cells, indicative of apoptosis. (Korean J Dermatol 2004;42(1):65~68)

교사클릭 : 클릭! 현장리포트 "좌충우돌 교사일기" ; 보람과 희망을 가꾸는 자리

최혜정 ( Choe Hye Jeong ) 장로회신학대학교 기독교교육연구원 2004 교육교회 Vol.322 No.-

학술발표회 분야별 발표일정 : 3) 수산이용가공,생물공학 분야 ; 구두발표: 추출용매를 달리한 해조류 추출물의 항산화 특성

김민형 ( Kim Min Hyeong ),최혜정 ( Choe Hye Jeong ),박수지 ( Park Su Ji ),정인학 ( Jeong In Hag ),김병목 ( Kim Byeong Mog ),이진경 ( Lee Jin Gyeong ),임영선 ( Im Yeong Seon ) 한국수산학회 2003 추계학술발표회 Vol.- No.-

S-PBL 학습방법을 적용한 통합간호실습 교과목 운영이 간호학생의 비판적 사고 성향, 간호과정 수행능력, 핵심기본간호술 수행자신감에 미치는 효과

강현주(Kang Hyun Ju),김미영(Kim Mi Young),최혜정(Choe Hye jeong) 학습자중심교과교육학회 2016 학습자중심교과교육연구 Vol.16 No.12

본 연구는 S-PBL 학습방법을 적용한 통합간호실습 교과목 운영이 간호학생의 비판적 사고 성향, 간호과정 수행능력, 핵심기본간호술 수행자신감에 미치는 효과를 확인하고자 수행되었다. 연구 설계는 단일군 사전사후설계에 의한 실험 연구로 연구대상은 G도 소재 일개 4년제 대학 간호학과 4학년 학생 54명이었으며 연구 기간은 2016년 4월 27일부터 2016년 6월 22일까지였다. 연구 결과 S-PBL 학습방법을 적용한 통합간호실습 교과목 운영 후 비판적 사고 성향(t=-5.30, p<.001), 간호과정 수행능력(t=-6.40, p<.001), 핵심기본간호술 수행자신감(t=-7.32, p<.001)의 점수가 모두 사전에 비해 사후에 유의하게 증가하였다. 본 연구를 통하여 S-PBL를 적용한 통합실습 교과목 운영이 간호학생들의 비판적 사고 능력과 간호과정 수행능력을 향상시키고 핵심기본간호술에 대한 자신감을 높이는 데 효과가 있음이 확인되었다. 향후 S-PBL 학습방법을 적용한 다양한 통합간호실습 교과목 운영에 대한 효과를 확인하는 실험 연구를 제언한다. This study was performed to examine the effects of integrated nursing practicum by applying simulation-problem based learning (S-PBL) on the critical thinking disposition, nursing process competence, and self-confidence on core basic nursing skills. A one group pre-posttest study was used. The participants were 54 nursing students who took the integrated nursing practicum in the first semester on 2016. The period for this study was from April 27th to June 22nd in 2016. The course included three nursing scenarios. For each scenario, it was applied the group discussion for nursing process (2hours), the practice for core basic nursing skills (4hours), and the group simulation practice and debriefing (2hours). Data were analyzed using the SPSS 22.0 version with descriptive statistics paired t-test, and Pearson correlation coefficients. After the course, there were significant increase in critical thinking disposition(t=-5.30, p<.001), nursing process competence(t=-6.40, p<.001), and self-confidence on core basic nursing skills(t=-7.32, p<.001). There was a significant positive correlation between post critical thinking disposition and post nursing process competence(r=632, p<.001). The findings of this study show that integrated nursing practicum by simulation-problem based learning can be used for improvement the critical thinking disposition, nursing process competence and self-confidence on core basic nursing skills of nursing students through nursing practicum.

한국 현대무용의 한국적 주제에 관한 고찰

김을교,최혜정 명지대학교 예체능연구소 1999 藝體能論集 Vol.10 No.-

This is a historical analysis to verify that westernized Korean contemporary dance has been tried to change it's image to express Korean's identity For the purpose of this study, three hypothetical facts such as theme of dance. background of correograph were investigated to know whether the three facts have included Korean's traditional image. Results of this study indicated as follows : Themes of Korean contemporary dance have been related and possessed Korean traditional cultures and images From the early stage, Korean culture were involved to themes of Korean contemporary dance. However, there was a barrier of Korean contemporary dance to eliminate western style and westernized image, that was a mainstreaming to mimic american contemporary dance from 1960 to 1980. However, there were many Korean dancers have tried to keep Korean traditional in their performance after the year of 1980. In the focus of using Korean cultures for the stage, many dancers have used Korean traditional items. Especially, using small theater and open stage has been provided opportunities to express Korean traditional cultures. Even though many Korean contemporary dancers created their works by using Korean traditional cultures and items, it was not enough to express unique characteristics of Korean image due to the lack of using unique dancing motions of Korean. Therefore, it is important, at first, to create unique dance motions to express Korean image.

인공모발 이식술 (Artificial Hair Implantation) 시행 후 발생한 피부 합병증 4예

최희창,최혜정,박영민,김형옥,김정원,장인강 대한피부과학회 2002 대한피부과학회지 Vol.40 No.12

The implantation of synthetic fibers into the human scalp to simulate natural hair has been shown to cause severe complications and be a possible danger to human health. The complications are frequent and include pain, pruritus, hemorrhage, infection, facial swelling. loss of natural hair, scarring and possible malignant degeneration of the surrounding scalp. We report herein four typical cases of severe cutaneous complications after artificial hair implantation. (Korean J Dermatol 2002;40(12) : 1594∼1597)