Al-Mg-Si 합금의 초기 석출물이 비대칭 압연에 의한 전단변형에 미치는 영향 : 집합조직과 성형성

채원기,김봉규,이종범,한준현 대한금속·재료학회 2020 대한금속·재료학회지 Vol.58 No.10

Al-Mg-Si alloy was rolled asymmetrically at several temperatures to apply shear deformation, and the effects of the initial precipitate on shear deformation, texture evolution, formability, and plastic anisotropy were studied. Texture was analyzed using a EBSD, and the formability and plastic anisotropy of the specimen were evaluated using the value and value calculated from the plastic strain ratio (r-value) which was determined from the change in the length of the specimen during tensile deformation. Asymmetric rolling induces a larger equivalent strain than symmetric rolling, and the equivalent strain increases as the asymmetric rolling temperature increases. When a specimen with peak-aged initial precipitates was asymmetrically rolled, less shear deformation occurred at room temperature than in a solution-treated specimen without initial precipitates. In contrast, a larger shear deformation occurred at high temperatures (500°C). With asymmetric rolling at room temperature, the specimens without initial precipitates had higher formability and lower plasticity, while for asymmetric rolling at high temperature, the specimens with initial precipitates had higher formability and lower plastic anisotropy. This is due to the <111>//ND texture, such as {111}<110> and {111}<112> orientation that has similar and high r-values at 0°, 45°, and 90° to the rolling direction, developed by the shear deformation that occurred during asymmetric rolling.

교육소갑문 : " 한 자루의 촛불 , 어떻게 사용할 것인가 " - 교육강사 황무굉 선생의 강의 중에서 -

채원기 대한지방행정공제회 2001 地方行政 Vol.50 No.573

초고압 GIS용, Epoxy/Micro Silica/Micro Alumina Mixture Composites의 전기적 특성에관한 연구

채원기(Won-ki Chae),김세훈(Se-Hoon kim),김수창(Su-Chang Kim),김승완(Seung-Wan Kim),방지원(Ji Won Bang),방극현(Geuk-Hyeon Bang),오영준(Young-Jun Oh),최유진(Yu-Jin Choi),최지현(Ji-Hyun Choi),나상호(Sang-Ho Na),표판식(Pan-Sik Pyo),이보인(B 대한전기학회 2022 대한전기학회 학술대회 논문집 Vol.2022 No.7

The Development of a New Soybean Strain Without Kunitz Trypsin Inhibitor, Lectin, and 7S α’ Subunit Protein

Won Gi Chae(채원기),Sang Woo Choi(최상우),Gyung Young Kang(강경영),Jong Il Chung(정종일) 한국생명과학회 2020 생명과학회지 Vol.30 No.7

성숙 콩[Glycine max (L.) Merr.] 종자는 약 40%의 단백질을 함유하고 있으며 아이소플라본, 사포닌, 루테인, 비타민등 다양한 기능성 성분을 함유하고 있다. 그러나, Kunitz Trypsin Inhibitor (KTI) 단백질, 렉틴 단백질, 7S α" 서버유닛 단백질이 함유되어져 있어 콩의 품질과 기능성을 저하시키고 있다. 본 연구는 콩 및 콩 제품의 품질과 기능성을 저하시키는 KTI, 렉틴 및 7S α" 서버유닛 3가지 단백질이 모두 유전적으로 결핍된 콩 계통(titilelecgy1cgy1 유전자형)을 선발하기 위하여 진행되었다. 3개의 모본(개척2호, PI506876, Le-16)을 이용한 유전집단으로부터 성숙종자에서 KTI, 렉틴 및 7S α" 서버유닛 3가지 단백질이 모두 없는 titilelecgy1cgy1 유전자형을 가진 1개의 계통을 개발하였다. 개발된 계통은 자주색 꽃, 유한신육형, 노란종피을 가지고 있으며 초장은 58 cm로 대원콩(46 cm)보다 길었다. 백립중은 27.1 g으로 대원콩(29.0 g)보다 작았다. 본 연구를 통하여 선발된 계통은 KTI, 렉틴 및 7S α" 서버유닛 3가지 단백질이 모두 없는 고품질 기능성 콩 품종 육성을 위한 중간모본으로 이용될 수 있을 것으로 사료되었다. Soybean [Glycine max (L.) Merr.] seeds contain an average of 40% protein on a dry weight basis, but they also contain antinutritional elements such as lectin, Kunitz trypsin inhibitor (KTI), and 7S α"- subunit protein. The objective of this research was to develop a new soybean genotype with triple recessive alleles for these elements. Three parents (Gaechuck#2, PI506876, and Le-16) were used to develop the genetic population, and the presence of lectin and KTI ㅔprotein was detected using Western blot while 7S α" subunit protein was detected using SDS-PAGE. One F3 plant strain with proper agronomical traits such as type, height, seed quality, and 100-seed weight was selected. The genotype of the developed strain is titilelecgy1cgy1, that is KTI, lectin, and 7S α" subunit protein free. The new strain has a purple flower, determinate growth habit, and light yellow pods at maturity. The seed has a buffer hilum and is yellow in color. The new strain’s height was 58 cm compared to the Daewonkong cultivar at 46 cm, and its 100-seed weight was 27.1 g, smaller than the Daewonkong at 29.0 g. This is the first new soybean strain with the titilelecgy1cgy1 genotype, and it can be used to improve yellow soybean cultivars of high quality and function.

환경차 리어플로어의 복합소재 적용 타당성 고찰

송원기(Wonki Song),채기상(Kisang-Chae) 한국자동차공학회 2016 한국자동차공학회 부문종합 학술대회 Vol.2016 No.5

갈색 종피를 가진 cgy1cgy1titi 유전자형 콩 육종

최상우,채원기,강경영,정종일 경상대학교 농업생명과학연구원 2019 농업생명과학연구 Vol.53 No.4

콩[Glycine max (L.) Merr.] 종자에는 수용성 탄수화물 11%, 단백질 40%, 지방 21% 정도의 3대 영양소가 골고루 함유 되어 있고, 콩 종실 단백질은 양과 질적인 면에서 식물성 단백질 중 가장 우수한 것으로 알려져 있다. 그러나, 성숙 콩 종실에는 Kunitz Trypsin Inhibitor (KTI) 단백질, P34 단백질, 7S 단백질 등 품질과 기능성을 떨어뜨리는 성분이 다수 존재하고 있다. 본 연구는 콩 및 콩 제품의 품 질과 기능성을 떨어뜨리는 성분인 7S α' subunit 단백질과 Kunitz Trypsin Inhibitor (KTI) 단백질이 유전적으로 없으면서 (cgy1cgy1titi 유전자형) 갈색종피 색을 가진 콩 계통을 선발하기 위하여 진행되었 다. 3개의 모본 (장려품종, PI506876, Gaechuck#2)을 이용한 육종집단으로부터 갈색종피, 노란자엽 및 7S α' subunit 단백질과 KTI 단백질이 없는 개체를 선발하였다. 선발개체에 대하여 7S α' subunit 단백질과 KTI 단백질의 유전적 고정과 농업형질 평가를 통하여 갈색 종피를 가지면서 cgy1cgy1titi 유전자형을 가져 7S α' subunit 단백질과 KTI 단백질이 없는 농업형질이 양호한 계통을 선발하였다. 선발된 계통의 초장은 65 cm 정도였으며 백립중은 24.2 g으로 중립이었으며 종피색은 갈색이고 제색은 하얀 색이며 성숙 자엽색은 노란색이었다. 본 연구를 통하여 선발된 계통은 7S α' subunit 단백질 및 Kunitz Trypsine Inhibitor (KTI) 단백질이 동시에 없는 유색콩 품종 육성을 위한 중간모본으로 이용 될 수 있을 것으로 사료되었다. Soybean [Glycine max (L.) Merr.] protein is excellent nutritional factors and is widely used for human and animal feed in the world. Also, antinutritional factors in the raw mature soybean are exist. The presence of antinutritional proteins requires that soybeans to be heat-treated at high temperature. Kunitz trypsin inhibitor (KTI) protein and β-conglycinin (7S α'-subunit) protein are main antinutritional factors in soybean seed. The genetic removal of the antinutritional factors will improve the nutritional value of soybean seed. The objective of this research was to breed new soybean strain (cgy1cgy1titi genotype) with brown seed coat, 7S α' subunit protein free, and KTI protein free. Three parents were used. Presence and absence of 7S α'-subunit and KTI proteins were detected by SDS electrophoresis and Western Blot technique, respectively. One new strain (cgy1cgy1titi genotype) with brown seed coat, lacking of 7S α'-subunit and KTI protein was selected from F4 plant population. New strain has purple flower, brown seed coat, yellow cotyledon, white hilum, 7S α'-subunit protein free, and KTI protein free. Plant height of new strain was 65 cm and 100-seed weight was 24.2 g. New strain selected in this research will be used to improve new cultivar with brown seed coat, 7S α'-subunit and KTI protein free.

GalUR 유전자를 이용한 비타민 C 증대 상추 (Lactuca sativa L.) 형질전환체 개발

임미영,조이남,채원기,박영수,민병환,한지학 한국식물생명공학회 2008 식물생명공학회지 Vol.35 No.2

L-Ascorbic acid (vitamin C) in vegetables is an essential component of human nutrition. The objective is to transform lettuce (Lactuca sativa L.) with GalUR gene that is involved in the vitamin C biosynthesis. The cotyledons of Hwoahong (Nongwoo Bio Co.) were used to induce the callus and shoot under the selection media with MS + 30 g/L Sucrose + 0.5 mg/L BAP + 0.1 mg/L NAA + 100 mg/L kanamycin + 200 mg/L lilacillin, pH 5.2. The shoot was developed from the cut side of the explants after 3 weeks on the selection media. We successfully transformed the lettuce with GalUR gene and analyzed the levels of vitamin C. We found that some of the lettuce transgenic lines contained higher levels of vitamin C compared with the normal one (non-transformed). Especially, some of T1 lettuces inserted by GalUR showed about 3~4 times higher content of vitamin C compared to the non-transformed lettuce. This data support the previously work performed with GLOase transgenic T1 lettuces from which several times higher content of vitamin C were identified. The T2 lettuces with high content of vitamin C have been selected for further analysis.

CYP2D6 allele frequencies in Korean population, comparisonwith East Asian, Caucasian and African populations,and the comparison of metabolic activity of CYP2D6 genotypes

변지영,김영훈,이충민,김세형,채원기,정의현,최창익,장춘곤,이석용,배정우,이윤정 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.9

Cytochrome P450 (CYP) 2D6 is present in less than about 2% of all CYP enzymes in the liver, but it is involved in the metabolism of about 25% of currently used drugs. CYP2D6 is the most polymorphic among the CYP enzymes. We determined alleles and genotypes of CYP2D6 in 3417 Koreans, compared the frequencies of CYP2D6 alleles with other populations, and observed the differences in pharmacokinetics of metoprolol, a prototype CYP2D6 substrate, depending on CYP2D6 genotype. A total of 3417 unrelated healthy subjects were recruited for the genotyping of CYP2D6 gene. Among them, 42 subjects with different CYP2D6 genotypes were enrolled in the pharmacokinetic study of metoprolol. The functional allele *1 and *2 were present in frequencies of 34.6 and 11.8%, respectively. In decreased functional alleles, *10 was the most frequent with 46.2% and *41 allele was present in 1.4%. The nonfunctional alleles *5 and *14 were present at 4.5 and 0.5% frequency, respectively. The *X 9 N allele was present at a frequency of 1.0%. CYP2D6*1/*1, *1/*2 and *2/*2 genotypes with normal enzyme activity were present in 12.1%, 8.6% and 1.4% of the subjects, respectively. CYP2D6*5/*5, *5/*14, and *14/*14 genotypes classified as poor metabolizer were only present in 4, 2, and 1 subjects, respectively. Mutant genotypes with frequencies of more than 1% were CYP2D6*1/*10 (32.0%), *10/*10 (22.3%), *2/*10 (11.7%), *5/*10 (3.7%), *1/*5 (2.5%), and *10/*41 (1.2%). The relative clearance of metoprolol in CYP2D6*1/*10, *1/*5, *10/*10, *5/*10, and *5/*5 genotypes were 69%, 57%, 24%, 14% and 9% of CYP2D6*wt/*wt genotype, respectively. These results will be very useful in establishing a strategy for precision medicine related to the genetic polymorphism of CYP2D6.

Effect of the CYP2D6*10 allele on the pharmacokinetics of clomiphene and its active metabolites

김미정,변지영,김영훈,김세형,이충민,정의현,채원기,이윤정,장춘곤,이석용,최창익 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.3

Clomiphene citrate, a selective estrogen receptormodulator, is metabolized into its 4-hydroxylated activemetabolites, primarily by CYP2D6. In this study, weinvestigated the effects of the most common CYP2D6variant allele in Asians, CYP2D6*10, on the pharmacokineticsof clomiphene and its two active metabolites (4-OHCLOand 4-OH-DE-CLO) in healthy Korean subjects. Asingle 50-mg oral dose of clomiphene citrate was given to22 Korean subjects divided into three genotype groupsaccording to CYP2D6 genotypes, CYP2D6*wt/*wt (n = 8;*wt = *1 or *2), CYP2D6*wt/*10 (n = 8) andCYP2D6*10/*10 (n = 6). Concentrations of clomipheneand its metabolites were determined using a validatedHPLC–MS/MS analytical method in plasma samples collectedup to 168 h after the drug intake. There was a significantdifference only in the Cmax of clomiphene betweenthree CYP2D6 genotype groups (p\0.05). Paradoxically,the elimination half-life (t1/2) and AUC of both activemetabolites were all significantly increased in theCYP2D6*10 homozygous carriers, compared with othergenotype groups (all p\0.001). The AUCinf of correctedclomiphene active moiety in CYP2D6*10/*10 subjects was2.95- and 2.05-fold higher than that of CYP2D6*wt/*wtand *wt/*10 genotype groups, respectively (bothp\0.001). Along with the partial impacts on the biotransformationof clomiphene and its metabolites byCYP2D6 genetic polymorphism, further studies on theeffects of other CYP enzymes in a multiple-dosing conditioncan provide more definite evidence for the inter-individualvariabilities in clomiphene pharmacokinetics and/ordrug response.

A Stack of Recessive Alleles of Kunitz Trypsin Inhibitor, Lectin, and Stachyose in Soybean

Sang Woo Choi(최상우),Won Gi Chae(채원기),Gyung Young Kang(강경영),Jong Il Chung(정종일) 한국생명과학회 2019 생명과학회지 Vol.29 No.7

성숙 콩[Glycine max (L.) Merr.] 종자에는 단백질, 지방, 탄수화물의 3대 영양소와 아이소플라본 등 다양한 기능성 성분이 함유되어 있다. 그러나, Kunitz Trypsin Inhibitor (KTI) 단백질, 렉틴 단백질, 난소화성 올리고당인 stachyose 성분이 함유되어져 있어 품질과 기능성을 저하시키고 있다. 본 연구는 콩 및 콩 제품의 품질과 기능성을 저하시키는 KTI 및 렉틴 단백질과 stachyose의 성분 함량과 관련된 유전자들이 모두 열성으로 작용하는 콩 계통(triple recessive genotype)을 선발하기 위하여 진행되었다. 3개의 모본(개척2호, PI200508, 14G20)을 이용한 육종집단으로부터 성숙 종자에서KTI 및 렉틴 단백질이 없으면서 stachyose의 함량이 일반콩보다 현저히 적은 triple recessive 유전자형(titilelers2rs2) 개체를 선발하였다. 선발된 계통의 초장은 51 cm 정도였으며 백립중은 31.0 g으로 대립이었으며 종피색 및 제색은 노란색이었다. Stachyose의 함량(g/kg)은 일반콩(13.5 g/kg)보다 훨씬 낮았다(3.8 g/kg). 본 연구를 통하여 선발된 계통은 KTI 단백질과 렉틴 단백질이 동시에 없으며 stachyose의 함량이 낮은 다양한 콩 품종 육성을 위한 중간모본으로 이용될 수 있을 것으로 사료되었다. Soybean [Glycine max (L.) Merr.] is one of the major food sources of protein, oil, carbohydrates, isoflavones, and other nutrients for both humans and animals. However, soybean seeds contain antinutritional factors, such as lectin protein, Kunitz Trypsin Inhibitor (KTI) protein, and stachyose. The objective of this research was to stack recessive alleles for development a triple recessive genotype, titilelers2rs2, with low KTI protein, lectin protein, and stachyose contents. Three parents (Gaechuck#2, PI200508, and 14G20) were used to develop the breeding population. The presence or absence of the lectin and KTI proteins was detected by western blotting. The stachyose content in mature seeds was determined by HPLC. Agronomic traits, such as plant type, plant height, maturity date, lodging, seed quality, and 100-seed weight, were evaluated for the four F3 plant strains. One F4 plant strain with the desired agronomical traits was selected. One new strain with the triple recessive titilelers2rs2 genotype was developed. The plant height of the new strain was 51 cm and the 100-seed weight was 31.0g. The new strain had a yellow seed coat and yellow hilum. The stachyose content of the new strain was 3.8 g/kg. One strain developed in this research will be used to produce improved yellow soybean cultivars that are free of lectin and KTI proteins and low in stachyose content.