Al-Mg-Si 합금의 초기 석출물이 비대칭 압연에 의한 전단변형에 미치는 영향 : 집합조직과 성형성

채원기,김봉규,이종범,한준현 대한금속·재료학회 2020 대한금속·재료학회지 Vol.58 No.10

Al-Mg-Si alloy was rolled asymmetrically at several temperatures to apply shear deformation, and the effects of the initial precipitate on shear deformation, texture evolution, formability, and plastic anisotropy were studied. Texture was analyzed using a EBSD, and the formability and plastic anisotropy of the specimen were evaluated using the value and value calculated from the plastic strain ratio (r-value) which was determined from the change in the length of the specimen during tensile deformation. Asymmetric rolling induces a larger equivalent strain than symmetric rolling, and the equivalent strain increases as the asymmetric rolling temperature increases. When a specimen with peak-aged initial precipitates was asymmetrically rolled, less shear deformation occurred at room temperature than in a solution-treated specimen without initial precipitates. In contrast, a larger shear deformation occurred at high temperatures (500°C). With asymmetric rolling at room temperature, the specimens without initial precipitates had higher formability and lower plasticity, while for asymmetric rolling at high temperature, the specimens with initial precipitates had higher formability and lower plastic anisotropy. This is due to the <111>//ND texture, such as {111}<110> and {111}<112> orientation that has similar and high r-values at 0°, 45°, and 90° to the rolling direction, developed by the shear deformation that occurred during asymmetric rolling.

교육소갑문 : 한 자루의 촛불 , 어떻게 사용할 것인가 - 교육강사 황무굉 선생의 강의 중에서 -

채원기 대한지방행정공제회 2001 지방행정 Vol.50 No.573

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초고압 GIS용, Epoxy/Micro Silica/Micro Alumina Mixture Composites의 전기적 특성에관한 연구

채원기(Won-ki Chae),김세훈(Se-Hoon kim),김수창(Su-Chang Kim),김승완(Seung-Wan Kim),방지원(Ji Won Bang),방극현(Geuk-Hyeon Bang),오영준(Young-Jun Oh),최유진(Yu-Jin Choi),최지현(Ji-Hyun Choi),나상호(Sang-Ho Na),표판식(Pan-Sik Pyo),이보인(B 대한전기학회 2022 대한전기학회 학술대회 논문집 Vol.2022 No.7

The Development of a New Soybean Strain Without Kunitz Trypsin Inhibitor, Lectin, and 7S α’ Subunit Protein

Won Gi Chae(채원기),Sang Woo Choi(최상우),Gyung Young Kang(강경영),Jong Il Chung(정종일) 한국생명과학회 2020 생명과학회지 Vol.30 No.7

성숙 콩[Glycine max (L.) Merr.] 종자는 약 40%의 단백질을 함유하고 있으며 아이소플라본, 사포닌, 루테인, 비타민등 다양한 기능성 성분을 함유하고 있다. 그러나, Kunitz Trypsin Inhibitor (KTI) 단백질, 렉틴 단백질, 7S α" 서버유닛 단백질이 함유되어져 있어 콩의 품질과 기능성을 저하시키고 있다. 본 연구는 콩 및 콩 제품의 품질과 기능성을 저하시키는 KTI, 렉틴 및 7S α" 서버유닛 3가지 단백질이 모두 유전적으로 결핍된 콩 계통(titilelecgy1cgy1 유전자형)을 선발하기 위하여 진행되었다. 3개의 모본(개척2호, PI506876, Le-16)을 이용한 유전집단으로부터 성숙종자에서 KTI, 렉틴 및 7S α" 서버유닛 3가지 단백질이 모두 없는 titilelecgy1cgy1 유전자형을 가진 1개의 계통을 개발하였다. 개발된 계통은 자주색 꽃, 유한신육형, 노란종피을 가지고 있으며 초장은 58 cm로 대원콩(46 cm)보다 길었다. 백립중은 27.1 g으로 대원콩(29.0 g)보다 작았다. 본 연구를 통하여 선발된 계통은 KTI, 렉틴 및 7S α" 서버유닛 3가지 단백질이 모두 없는 고품질 기능성 콩 품종 육성을 위한 중간모본으로 이용될 수 있을 것으로 사료되었다. Soybean [Glycine max (L.) Merr.] seeds contain an average of 40% protein on a dry weight basis, but they also contain antinutritional elements such as lectin, Kunitz trypsin inhibitor (KTI), and 7S α"- subunit protein. The objective of this research was to develop a new soybean genotype with triple recessive alleles for these elements. Three parents (Gaechuck#2, PI506876, and Le-16) were used to develop the genetic population, and the presence of lectin and KTI ㅔprotein was detected using Western blot while 7S α" subunit protein was detected using SDS-PAGE. One F3 plant strain with proper agronomical traits such as type, height, seed quality, and 100-seed weight was selected. The genotype of the developed strain is titilelecgy1cgy1, that is KTI, lectin, and 7S α" subunit protein free. The new strain has a purple flower, determinate growth habit, and light yellow pods at maturity. The seed has a buffer hilum and is yellow in color. The new strain’s height was 58 cm compared to the Daewonkong cultivar at 46 cm, and its 100-seed weight was 27.1 g, smaller than the Daewonkong at 29.0 g. This is the first new soybean strain with the titilelecgy1cgy1 genotype, and it can be used to improve yellow soybean cultivars of high quality and function.

환경차 리어플로어의 복합소재 적용 타당성 고찰

송원기(Wonki Song),채기상(Kisang-Chae) 한국자동차공학회 2016 한국자동차공학회 부문종합 학술대회 Vol.2016 No.5

갈색 종피를 가진 cgy1cgy1titi 유전자형 콩 육종

최상우,채원기,강경영,정종일 경상국립대학교 농업생명과학연구원 2019 농업생명과학연구 Vol.53 No.4

GalUR 유전자를 이용한 비타민 C 증대 상추 (Lactuca sativa L.) 형질전환체 개발

임미영,조이남,채원기,박영수,민병환,한지학 한국식물생명공학회 2008 식물생명공학회지 Vol.35 No.2

L-Ascorbic acid (vitamin C) in vegetables is an essential component of human nutrition. The objective is to transform lettuce (Lactuca sativa L.) with GalUR gene that is involved in the vitamin C biosynthesis. The cotyledons of Hwoahong (Nongwoo Bio Co.) were used to induce the callus and shoot under the selection media with MS + 30 g/L Sucrose + 0.5 mg/L BAP + 0.1 mg/L NAA + 100 mg/L kanamycin + 200 mg/L lilacillin, pH 5.2. The shoot was developed from the cut side of the explants after 3 weeks on the selection media. We successfully transformed the lettuce with GalUR gene and analyzed the levels of vitamin C. We found that some of the lettuce transgenic lines contained higher levels of vitamin C compared with the normal one (non-transformed). Especially, some of T1 lettuces inserted by GalUR showed about 3~4 times higher content of vitamin C compared to the non-transformed lettuce. This data support the previously work performed with GLOase transgenic T1 lettuces from which several times higher content of vitamin C were identified. The T2 lettuces with high content of vitamin C have been selected for further analysis.

CYP2D6 allele frequencies in Korean population, comparisonwith East Asian, Caucasian and African populations,and the comparison of metabolic activity of CYP2D6 genotypes

변지영,김영훈,이충민,김세형,채원기,정의현,최창익,장춘곤,이석용,배정우,이윤정 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.9

Cytochrome P450 (CYP) 2D6 is present in less than about 2% of all CYP enzymes in the liver, but it is involved in the metabolism of about 25% of currently used drugs. CYP2D6 is the most polymorphic among the CYP enzymes. We determined alleles and genotypes of CYP2D6 in 3417 Koreans, compared the frequencies of CYP2D6 alleles with other populations, and observed the differences in pharmacokinetics of metoprolol, a prototype CYP2D6 substrate, depending on CYP2D6 genotype. A total of 3417 unrelated healthy subjects were recruited for the genotyping of CYP2D6 gene. Among them, 42 subjects with different CYP2D6 genotypes were enrolled in the pharmacokinetic study of metoprolol. The functional allele *1 and *2 were present in frequencies of 34.6 and 11.8%, respectively. In decreased functional alleles, *10 was the most frequent with 46.2% and *41 allele was present in 1.4%. The nonfunctional alleles *5 and *14 were present at 4.5 and 0.5% frequency, respectively. The *X 9 N allele was present at a frequency of 1.0%. CYP2D6*1/*1, *1/*2 and *2/*2 genotypes with normal enzyme activity were present in 12.1%, 8.6% and 1.4% of the subjects, respectively. CYP2D6*5/*5, *5/*14, and *14/*14 genotypes classified as poor metabolizer were only present in 4, 2, and 1 subjects, respectively. Mutant genotypes with frequencies of more than 1% were CYP2D6*1/*10 (32.0%), *10/*10 (22.3%), *2/*10 (11.7%), *5/*10 (3.7%), *1/*5 (2.5%), and *10/*41 (1.2%). The relative clearance of metoprolol in CYP2D6*1/*10, *1/*5, *10/*10, *5/*10, and *5/*5 genotypes were 69%, 57%, 24%, 14% and 9% of CYP2D6*wt/*wt genotype, respectively. These results will be very useful in establishing a strategy for precision medicine related to the genetic polymorphism of CYP2D6.

Effect of the CYP2D6*10 allele on the pharmacokinetics of clomiphene and its active metabolites

김미정,변지영,김영훈,김세형,이충민,정의현,채원기,이윤정,장춘곤,이석용,최창익 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.3

Clomiphene citrate, a selective estrogen receptormodulator, is metabolized into its 4-hydroxylated activemetabolites, primarily by CYP2D6. In this study, weinvestigated the effects of the most common CYP2D6variant allele in Asians, CYP2D6*10, on the pharmacokineticsof clomiphene and its two active metabolites (4-OHCLOand 4-OH-DE-CLO) in healthy Korean subjects. Asingle 50-mg oral dose of clomiphene citrate was given to22 Korean subjects divided into three genotype groupsaccording to CYP2D6 genotypes, CYP2D6*wt/*wt (n = 8;*wt = *1 or *2), CYP2D6*wt/*10 (n = 8) andCYP2D6*10/*10 (n = 6). Concentrations of clomipheneand its metabolites were determined using a validatedHPLC–MS/MS analytical method in plasma samples collectedup to 168 h after the drug intake. There was a significantdifference only in the Cmax of clomiphene betweenthree CYP2D6 genotype groups (p\0.05). Paradoxically,the elimination half-life (t1/2) and AUC of both activemetabolites were all significantly increased in theCYP2D6*10 homozygous carriers, compared with othergenotype groups (all p\0.001). The AUCinf of correctedclomiphene active moiety in CYP2D6*10/*10 subjects was2.95- and 2.05-fold higher than that of CYP2D6*wt/*wtand *wt/*10 genotype groups, respectively (bothp\0.001). Along with the partial impacts on the biotransformationof clomiphene and its metabolites byCYP2D6 genetic polymorphism, further studies on theeffects of other CYP enzymes in a multiple-dosing conditioncan provide more definite evidence for the inter-individualvariabilities in clomiphene pharmacokinetics and/ordrug response.

Simultaneous determination of tolterodine and its two metabolites, 5-hydroxymethyltolterodine and N-dealkyltolterodine in human plasma using LC–MS/MS and its application to a pharmacokinetic study

김영훈,변지영,김세형,이충민,정의현,채원기,장춘곤,이석용,이윤정 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.11

Tolterodine is a nonselective muscarinic antagonistthat is indicated for the overactive urinary bladder andother urinary difficulties. We developed and validated asimple, rapid and sensitive high-performance liquid chromatographyanalytical method utilizing tandem massspectrometry (LC–MS/MS) for the quantitation of tolterodineand its major metabolites, 5-hydroxymethyltolterodine(5-HMT) and N-dealkyltolterodine (NDT), inhuman plasma. After liquid–liquid extraction with methylt-butyl ether, chromatographic separation of the threeanalytes was achieved using a reversed-phase Luna Phenylhexylcolumn (100 9 2.0 mm, 3 lm particles) with amobile phase of 10 mM ammonium formate buffer (pH3.5)-methanol (10:90, v/v) and quantified by MS/MSdetection in electrospray ionization (ESI) positive ionmode. The retention time of tolterodine, 5-HMT, NDT, andinternal standard (IS) were 1.4, 1.24, 1.33, and 1.26 min,respectively. The calibration curves were linear over arange of 0.025–10 ng/ml for tolterodine and 5-HMT, and0.05–10 ng/ml for NDT. The lower limit of quantificationsusing 200 ll of human plasma was 0.025 ng/ml for tolterodineand 5-HMT, and 0.05 ng/ml for NDT. The meanaccuracy and precision for intra- and inter-run validation oftolterodine, 5-HMT, and NDT were all within acceptablelimits. These results showed that a simple, rapid andsensitive LC–MS/MS method for the quantification oftolterodine and its major metabolites in human plasma wasdeveloped. This method was successfully applied to apharmacokinetic study in humans.