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운동성 산화 스트레스와 항산화비타민의 보충이 말초단핵세포의 NF-kB 활성에 미치는 영향
진영수,박건구,박준영,김미정,이왕록,김혜영,이한준,박은경 대한스포츠의학회 2000 대한스포츠의학회지 Vol.18 No.2
Reactive oxygen species(ROS) are implicated in the pathogenesis of a wide variety of human diseases. Numerous studies indicate that ROS may serve as common intracellular molecules that contribute to the process of nuclear factor(NF)κB activation in response to a diverse stimuli. In our laboratory, we have demonstrated tat antioxidants could reverse the decline of immune function caused by exercise-induced ROS. Furthermore, it in necessary to understand a mechanism underlying ROS-dependent disorder in biological system. Recent studies have been shown that several gene expression were regulated by oxidants, antioxidants and other determinants of the intracellular reduction-oxidation(redox) state. In this process, NF-κB have been shown to play a important role. The purpose of the present investigation was to evaluate the effect of exercise-induced oxidative stress and antioxidnt supplementation on NF-κB activation in peripheral mononuclear cells. Forty male SD rats(4 weeks old) were randomly divided into noraml diet group and antioxidants(ATO) supplement group, and then ATO groups were treated with antioxidants(VE: 2001U/kg, VC: 50mg/rat, β-carotene: 300mg/kg, vitamin B6: 250㎍/100g, selenomethionine: 0.1mg/kg) for 16 weeks. After 16 weeks breeding at each condition, each group divided into two groups: Control group(CR) fed generally, Exercise group(CE) fed generally and followed by acute exercise 16 weeks later. Antioxidant Antioxidants and Exercise group(AE) fed with antioxidants and followed by acute exercise. The activation of NF-κB binding activity was increased after exhaustive exercise bout in both group. In addition, pretreatment of ATO group with the antioxidants mixture lead to the inhibition of NF-κB binding activity. This results suggest that NF-κB activation should be further studies in response to a variety of exercise.
진영수,김미정,박준영,김근수,박정태,김용권 대한스포츠의학회 1998 대한스포츠의학회지 Vol.16 No.2
The purpose of this study was to investigate the effects of sustained exercise above and below anaerobic threshold(AT) on exercise-induced acute phase response of T lymphocytes and natural killer cells(NK cell). The Eight healthy graduate students(M. 27.9±2.2 yr) were participated in the exercise sessions. Subjects had no known medical problems and were not taking any medications. All subjects were participated in three exercise session on a treadmill; and initial maximal incremental exercise test for determination of anaerobic threshold, and two subsequent bouts that subjects exercise for 40min at 80%-AT level(below AT level: BAT) and the same amount(301.3±20.5kcal) of exercise at 110%-AT level(above AT level: AAT). Venous blood samples were drown at the forearm antecubital vein at pre-exercise, post-exercise, and 30min/90min recovery(15ml each). Using anti-CD3 and NK cells (CD3-negative/ CD16+CD56 positive) were enumerated with flowcytometery. In this study, mitogen(PHA)-stimulated T lymphocyte proliferation response and NK cell cytotoxic activity(NKCA) against target cell(K562) were measured to determine the immune cell functions. There was a significant interactions between intensity(AAT vs BAT) and time in T lymphocytes(CD3+) and NK cell(CD3-/CD16+CD56+) counts(T lymphocytes, P=0.017; NK cells, P=0.049). Also, there were significant changes between all of each sampling interval in AAT although any significant changes existed in BAT on CD3+ cell number. In changing patterns of CD3-/CD16+CD56+ cell counts, AAT was greater than BAT. After AAT, T lymphocyte proliferative response was significantly decrease at postexercise(P=0.017), although there was not significant change after BAT. Also, it is appeared that faster recovery pattern followed after BAT than AAT. NKCA was higher after sustained exercise in below AT level(30min rec, P=0.002; 90min rec, P=0.006) than AAT during recovery. Thus sustained exercise at slightly AAT resulted in a significant immunosuppressions in T lymphocytes and NK cells. Exercise in BAT can avoiding these immonological disturbance and may decrease chances to undesirable effect of exercise in immune system.