누룩 중의 젖산균의 분리 및 동정

조갑연(Kab Yeon Jo),하덕모(Duk Mo Ha) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.2

The distribution of lactic acid bacteria and total bacteria in 27 samples of Nuruk (traditional Korean rice wine starter) were investigated, and lactic acid bacteria isolated from those samples were identified. The average number of lactic acid bacteria and total bacteria were 2,11×10^7 and 2.08×10^8 cfu/g, respectively. There was no appreciable differences in the number of lactic acid bacteria among those samples, meanwhile the number of total bacteria varied significantly from sample to sample. Among the lactic acid bacteria, more cocci were counted than rods in those samples. Thirty-two strains of lactic acid bacteria obtained from those samples were identified as Leuconostoc mesenteroides subsp. mesenteroides (11 strains), Pediococcus acidilactici (7 strains), Lactobacillus plantarum (3 strains), L. murinus (7 strains) and Enterococcus faecium (4 strains).

Type 2 Isopentenyl Diphosphate Isomerase로서 Synechocystis sp. PCC6803의 sll1556의 작용특성

조갑연 ( Kab Yeon Cho ) 한국식품영양학회 2010 韓國食品營養學會誌 Vol.23 No.4

Isopentenyl diphosphate(IPP) isomerization to dimethylallyl diphosphate(DMAPP) is an important step for the efficient production of isoprenoids such as lycopene, β-carotene, astaxanthin, etc. The type Ⅱ isopentenyl diphosphate isomerase gene from Synechocystis sp. PCC6803(sll1556, Syidi2) was cloned and expressed in Escherichia coli DH5α. When E. coli DH5α harboring lycopene synthesis genes, crtE, crtB, and crtI and mevalonate pathway genes, MvK1, MvK2, and Mvd, was cultured on LB medium containing mevalonate, the strain grew very slowly be due to the toxicity of isopentenyl diphosphate derived from mevalonate. When Syidi2 was introduced to E. coli DH5α harboring the lycopene synthesis genes and mevalonate pathway genes, growth on mevalonate medium was fully restored and the colony showed red color indicating lycopene formation. The growth rate of the mutant strain, E. coli DH5α(idi::Δkm), was very slow because of IPP accumulation and DMAPP deprivation. Ultimately the idi mutant was complemented by introducing the Syidi2 gene.

Activity Change of Ornithine Decarboxylase (ODC) after Hepatectomy

조갑연(Kab-Yeon Jou),성창근(Chang Guen Sung),하덕모(Duk-Mo Ha) 한국식품영양과학회 1995 한국식품영양과학회지 Vol.24 No.3

오르니틴 탈탄산효소(ODC)는 putrescine, spermine, 그리고 spermidine과 같은 polyamine을 생산하는 생합성기작의 첫단계이고 또한 율속단계에 있는 효소이다. 그리고 이 효소는 간이 재생될 때 갑작스럽게 효소의 역가가 증가한다고 알려져 있다. 본 연구에서는 생쥐의 간을 부분 절제수술 후에는 오르니틴 탈탄산효소와 mRNA의 변화가 급격하게 증가하였으나, 간을 절제하지 않은 수술에서는 mRNA의 양적인 변화가 없었으나 효소역가는 훨씬 감소된 값을 보였다. 그러나 mRNA와 효소역가의 무변화를 보였다는 여러 연구와는 상반된 결과를보여주고 있다. 그러므로 보다 세밀한 분석은 수술 후 음식물의 투여가본 효소의 역가변화 그리고 mRNA의 양적 변화에 미치는 영향에 대한 연구가 필요하다 하겠다. Ornithine decarboxylase (ODC) catalyzes the first and key step in the polyamine biosynthetic pathway. Ornithine decarboxylase is known to the enzyme that increase substantially its activity in regenerating liver. We found that activity and mRNA level for ODC increase significantly after partial hepatectomy in the rat. After laparotomy, there was significant decrease in activity ; however, mRNA content was unaltered in contrast to previous reports of no change in ornithine decarboxylase and thymidine kinase after sham hepatectomy. This may be mediated by the decrease in food intake after hepatectomy. Therefore it is necessary to examine the effect of food intake after hepatectomy on the ODC activity and mRNA level in the future.

한국 재래식 누룩 중의 곰팡이의 분리 및 동정

조갑연(Gab-Yeon Jo),이철우(Chul-Woo Lee) 한국식품영양과학회 1997 한국식품영양과학회지 Vol.26 No.5

56개의 누룩 시료로부터 곰팡이 111균주를 분리하였으며 이들 균주는 Aspergillus oryzae(12균주), Asp. parasiticus(8균주), Asp. fumigatus(3균주), Asp. ochraceus(7균주), Asp. wentii(5균주), Asp. niger(5균주), Asp. penicilloides(3균주), Asp. clavatus(4균주), Rhizopus oryzae(14균주), R. oligosporus(8균주), R. nigricans(5균주), R. arrhizus(5균주), Penicillium purpurogenum(2균주), Pen. rugulosum(1균주), Pen. citrinum(2균주), Pen. spinulosum(2균주), Pen. sclerotigenum(1균주), Pen. glabrum(1균주), Pen. granulatum(1균주), Pen. fellutanum(1균주), Geotrichum candidum(2균주), Absidia corymbifera(12균주), Mucor racemosus(2균주), M. plumbeus(2균주), 및 Curvularia lunatus(3균주)의 7속 25종으로 동정하였다. 이와같이 누룩 중의 주요 곰팡이는 Aspergillus속, Rhizopus속이며, Aspergillus속 균주가 가장 높은 빈도로 분리되었다. From Nuruks, a traditional Korean starter for rice wine, which collected from 42 different areas in Korea, 111 fungal strains were isolated. These isolates were identified as 25 species belonging to seven genera of Rhizopus oryzae(14 strains), R. oligosporus(8 strains), R. nigricans(5 strains), R. arrhizus(5 strains), Aspergillus oryzae(12 strains), Asp. parasiticus(8 strains), Asp. fumigatus(3 strains), Asp. ochraceus(7 strains), Asp. wentii(5 strains), Asp. niger(5 strains), Asp. penicilloides(3 strains), Asp. clavatus(4 strains), Penicillium purpurogenum(2 strains), Pen. rugulosum(1 strain), Pen. citrinum(2 strains), Pen. spinulosum(2 strains), Pen. sclerotigenum(1 strain), Pen. glabrum(1 strain), Pen. granulatum(1 strain), Pen. fellutanum(1 strain), Geotrichum candidum(2 strains), Absidia corymbifera(12 strains), Mucor racemosus(2 strains), M. plumbeus(2 strains) and Curvularia lunatus(3 strains).

튀김 옷의 종류가 부서짐성에 미치는 영향

이종혁,장규섭,박상욱,김동우,신두호,조갑연,홍순택 한국산업식품공학회 2001 산업 식품공학 Vol.5 No.1

콜히친 침지처리에 의한 ‘망종화’의 4배체 식물유도

권수정,조갑연,김학현 한국자원식물학회 2013 한국자원식물학회지 Vol.26 No.2

This study aimed to get the basic data on the breeding of good varieties in Hypericum patulum Thunberg. Theoptimum materials, concentration and soaking time were examined to identify the effective approach to induce tetraploidplant by colchicine treatment to cultivate the varieties. For the seed germination rate of seed by colchicine treatment, thehigher colchicine concentration was and the longer soaking time was, the more the germination rate decreased. Whileindividuals were germinated in 16 test groups except control group (no treatment group), all the plants were diploid and notetraploid was induced. For the plant regeneration rate by colchicine treatment on the explant of Hypericum patulumThunberg that was under in vitro culture, the higher the colchicine concentration increased, the ress the regeneration rate. While total 147 individuals were regenerated in all treatment, when the explant was soaking treatment in more than 0.05%for over 6 hours, tetraploid could be obtained. In the soaking treatment of 0.05% for over 6 hours, tetraploid could beobtained. In particular, for the soaking treatment in 0.05% for 12 hours, 8 tetraploids were induced, which was about 47.1%of the number of plant regenerated. In accordance with the observation on doubling of DNA contents in leaf in order toidentify polyploidy, the peak DNA content of G1 phase was 94.5 for diploid and 192.5 for tetraploid. It confirmed doublingof DNA content. Furthermore, the number of chloroplasts per guard cell depending on polyploid was around 10 in diploidand 17 to 19 in tetraploid, which were around 1.7 to 1.9 times as much as diploid. ‘망종화’에서 콜히친 처리에 의한 효율적인 4배체 식물을 유도하고자 적정 식물체 부위, 적정농도 및 침지시간을검토하였다. 종자의 발아율은 콜히친의 농도가 높을수록또한 침지시간이 길수록 저하되었다. 대조구를 제외한 16개의 처리구에서 총 453개체가 발아된 반면 4배체는 유도되지 않았다. 기내 배양 중인 줄기 절편체의 식물체 재생율은 콜히친 처리농도 0.01%에서 최고를 나타내다가 0.1%농도 이상으로 높아질수록 낮았다. 적정식물체 부위는 줄기 절편체로 나타났다. 4배체 식물은 콜히친을 0.05% 이상으로 6시간 침지처리 하였을 때 얻을 수 있었고, 특히0.05%, 12시간 침지처리에서 식물체의 재생수 대비 약42%의 높은 획득율을 보였다. Flowcytometry에 의해DNA함량의 배가여부를 확인한 결과, G1 phase의 DNA 함량 peak가 2배체에서 94.5, 4배체는 192.5로, DNA가 배가됨을 확인할 수 있었다. 또한 공변세포 당 엽록체 수는2배체가 약 10개인 것에 비해 4배체는 17∼19개로 2배체보다 약 1.7∼1.9배 정도 많았다.

‘녹색 꽃잎 도라지’의 기관분화에 미치는 배지조성 및 생 장조절제의 영향

권수정,조갑연,김학현 한국자원식물학회 2014 한국자원식물학회지 Vol.27 No.1

This study was carried out to determine the optimal medium composition and growth regulators for the micropropagation of Platycodon grandiflorum (Jacq.) A. DC. Nodes containing yellow green petals were used as plant materials to execute the study. The best performance of adventitious root development was found in 1/4 strength of MS basal salt and the growth was satisfactory in the concentration of 1/2 MS medium. The best condition for adventitious root development and growth was observed in the higher concentration (5%) of sucrose and activated charcoal free 1/4MS medium respectively. Adventitious roots were developed at the controlled culture medium at pH 4.8 with a tendency of suppression with higher levels of pH. However, it was prevailed that the development and growth depended on the concentration of agar. The lower concentration of agar (0.4%) was performed better than that of higher concentration (1.2%), whereas the agar concentration (0.4%) showed the best performance for the development and growth of adventitious roots. For the development of shoots containing node, BA combined with IAA was more effective than kinetin with IAA or NAA. The highest shoot development (3.9 shoots per explant) was performed on MS medium supplemented with 0.1 ㎎/L BA and 0.5 ㎎/L IAA. 본 연구는 ‘녹색 꽃잎 도라지’의 기내배양 시 배지구성물질의 적정농도 구명에 의한 대량번식을 목적으로 실시하였다. ‘녹색 꽃잎 도라지’의 절을 배양재료로 배양조건은 MS배지의 여러 가지 구성물질의 농도를 달리한 결과, 1/4MS 배지에서 가장 양호한 부정근의 형성을 보였으나 생장은 1/2MS배지에서 좋았다. Sucrose첨가는 농도가 높을수록 신초와 부정근의 형성 및 생장이 좋았다. 활성탄은 무첨가구에서 가장 많은 부정근의 형성과 양호한 생장을 보였다. 배지의 pH는 4.8로 조절된 배지에서 가장 많은 부정근을 형성하였으며, pH가 높아질수록 그 형성은 낮아지는 경향을 보였고, 부정근과 신초의 생장 또한 pH 4.8에서 가장 왕성하였다. Agar 농도별 실험에서 부정근의 형성과 생장은 그 농도가 낮아질수록 양호한 경향을 보여 가장 낮은 첨가구인 0.4% 농도구에서 가장 많은 부정근의 형성과 왕성한 생장을 보였다. 생장조절제를 혼용 첨가한 경우 신초의 형성은 BA와 IAA의 혼용구가 kinetin과 IAA 또는 NAA 혼용구에 비해 효과적이었으며, BA 0.1 ㎎/L와 IAA 0.5 ㎎/L 혼용구에서 절편체당 3.9개로 가장 많은 신초가 형성되었다.

人蔘紛 添加給食이 白鼠臟器中 核酸含量에 미치는 影響

金亨烈,趙甲衍,李成東 高麗大學校 倂設 保健大學 保健科學硏究所 1987 保健科學論集 Vol.15 No.1

This study was devised to observe the effects on the nucleic acid metabolism of Albino rats by feeding of diet supplemented with 2% to 8% ginseng root powder. The rats(50 heads), weighing 94 to 100g, were divided into five diet group, which were 1) stock diet as control, 2) 2% ginseng diet, 3) 4% ginseng diet, 4) 6% ginseng diet and 5) 8% ginseng diet. The each animal diet group was maintained with corresponding diet for 30 days and then sacrificed. The contents of nucleic acid in the liver, testis and brain were analyzed. In the view of the result, the nucleic acid metabolism in the rat was influenced by feeding of the diet supplemented with ginseng powder.

人蔘副産物 茶類가 白鼠의 蛋白質效率과 血淸蛋白質 含量에 미치는 影響

李成東,金亨烈,趙甲衍 高麗大學校 倂設 保健大學 保健科學硏究所 1985 保健科學論集 Vol.14 No.1

This study was designed to observe the protein nutritional effects of the boiling water 2g% extracts of panax ginseng -by-product teas (*Rootlet tea, *Processed ginseng leaf tea, *Processed ginseng stem, leaf and rootlet (Mixed tea)) on male rats(30 heads), Spragu-Dowley strain weighing 79±7g. They were divided into 4 dietary feeding group, and fed each corresponding extracts to the rats for 4 weeks. The protein efficiency and serum globulin contents in the group fed on panax ginseng-by-product teas extracts were all lower than that of the control group, however there was no significant difference among them. And by feeding ginseng mixed tea, the total protein contents in serum was decreased(p<0.05-0.02) but the A/G ratio was increased (p<0.05) compared with control group.

〈종 합 토 의〉

권순형,이현옥,조갑연,정낙현,손규목,정두례 한국식품영양학회 2012 한국식품영양학회 학술대회논문집(학술심포지움) Vol.2012 No.06