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정용훈,박경원,김민섭,오현직,최동희,안진철,이샛별,나규환,Min Byung Soh,김진아,정석 한국바이오칩학회 2023 BioChip Journal Vol.17 No.2
With the advances in organoid culture, patient-derived organoids are utilized in diverse fields to broaden our understanding of conventional 2-dimensional (2D) culture methods and animal models. Patient-derived organoids have found new applications, such as screening for patient-matching drugs, immune checkpoint drugs, and mutation-target drugs, in the field of drug screening. However, conventional dome-shaped Matrigel drop-based screening methods using 24-, 48-, and 96-well plates are not effective for carrying out large-scale drug screening using organoids. Here, we present a newly developed 96-well plate-based method for the effective screening of patient-derived tumor organoids embedded in Matrigel. The new screening plate has a central hole with a diameter of 3 or 5 mm to provide a definite space for placing Matrigel in a cylindrical shape. Compared to the conventional dome-shaped Matrigel where the Matrigel drop is located arbitrarily, a cylinder-shaped Matrigel position in confined central wells allowed for faster and cost-effective tumor organoid screening. Importantly, the cylinder-shaped Matrigel ensures better consistency in high-throughput image-based analysis, which is used worldwide. Our results demonstrate the possibility of replacing the conventional 24-, 48-, and 96-well plates with the newly developed plates for effective tumor organoid screening.
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한국의 CO<sub>2</sub> 배출, 경제성장 및 에너지믹스와의 관계 분석
정용훈,김수이,Jeong, Yong-Hun,Kim, Su-Lee 한국자원경제학회 2012 자원·환경경제연구 Vol.21 No.2
The relationship between environment and economic growth has been controversial for a long time. The cores of controversy are endogeneity problem and omitted variable bias. This paper tests EKC (Environmental Kuznets Curves) hypothesis by considering econometric issues and estimates the effects of energy mix on $CO_2$ emissions empirically and tests with time series during 1981~2008. By the results of this analysis, we convince EKC Hypothesis which the relationship between $CO_2$ emissions and economic growth is the inverted U-shaped and the national energy mix contributes significantly to GHG mitigation. We also find that the nuclear energy has the greatest contribution for $CO_2$ mitigation and the renewable energy does not seem to contribute little to the $CO_2$ mitigation because the proportion of renewable energy in Korea is negligible. In terms of final energy consumption, $CO_2$ increases and transportation sector is statistically and significantly associated.
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