배양한 흰쥐 대뇌세포의 저산소증 모델에서 황금(黃芩)이 유전자 표현에 미치는 영향

정승현,신길조,이원철,김성배,Chung, Sung-Hyun,Shin, Gil-Cho,Lee, Won-Chul,Kim, Sung-Bae 대한한방내과학회 2004 大韓韓方內科學會誌 Vol.25 No.4

Objectives : The purpose of this investigation is to evaluate the effects of Scutellaria baicalensis GEORGI on alteration in gene expression in a hypoxia model using cultured rat cortical cells. Methods : E18 rat cortical cells were grown in a Neurobasal medium containing B27 supplement. On 12 DIV, Scutellaria baicalensis GEORGI(20 ug/ml) was added to the culture media and left for 24 hrs. On 11 DIV, cells were given a hypoxic insult $(2%\;O_2/5%\;CO_2,\;37^{\circ}C,\;3\;hrs)$, returned to normoxia and cultured for another 24 hrs. Total RNA was prepared from Scutellaria baicalensis GEORGI-untreated (control) and -treated cultures and alteration in gene expression was analysed by microarray using rat 5K-TwinChips. Results : For most of the genes altered in expression, the Global M values were between -0.5 to +0.5. Among these, 1143 genes increased in their expression by more than Global M +0.1, while 1161 genes decreased by more than Global M -0.1. Effects on some of the genes whose functions are implicated in neural viability are as follows: 1) The expression of apoptosis-related genes such as Bad (Global M = 0.39), programmed cell death-2(Pdcd2) (Global M = 0.20) increased, while Purinergic receptor P2X(P2rxl) Global M = -0.22), Bc12-like1(Bc1211)(Global M = -0.19) decreased. 2) The expression of 'response to stress-related genes such as antioxidation-related AMP-activated protein kinase subunit gamma 1 gene (Prkag1) (Global M = 0.14), catalase gene (Global M = 0.14) and Heme Oxygenase(Hmoxl) increased. 3) The expression of Fos like antigen 2 (Fos12) expressed in neurons that survive ischemic insult increased (Global M = 0.97). Conclusions : these data suggest that Scutellaria baicalensis GEORGI increases the expression of antiapoptosis- and antioxidation- related genes in a way that can not yet be explained.

Dieckol Suppresses CoCl2-induced Angiogenesis in Endothelial Cells

정승현,장인승,전유진,김영목,박선주 한국수산과학회 2014 Fisheries and Aquatic Sciences Vol.17 No.3

Dieckol is a polyphenol compound isolated from brown algae that has anti-oxidant, anti-inflammatory, and anti-tumor activity. We examined the anti-angiogenic effects of dieckol in endothelial cells under hypoxic conditions. Treatment with CoCl2, a hypoxic mimetic agent, increased proliferation, adhesion, migration, and tube formation in HUVECs, as well as vessel sprouting in rat aortic rings, which correlated well with increased expression of hypoxia-inducible factor 1-alpha (HIF1α) and β1-integrin. Dieckol suppressed CoCl2-induced adhesion, migration, and tube formation in HUVECs and vessel sprouting in rat aortic rings. Dieckol treatment decreased CoCl2-induced overexpression of HIF1α and its downstream signaling molecules, including β1-integrin/Fak, Akt/eNOS, and p38 MAPK. These results suggest that dieckol is a novel angiogenesis inhibitor and a potential treatment for angiogenesis-dependent diseases in humans, such as malignant tumors.

카레분 및 향신료 추출물의 항 돌연변이 효과

정승현,정명수,이진선,박기문 한국축산식품학회 2002 한국축산식품학회지 Vol.22 No.4

Antimutagenic effects of extracts from curry powder and its individual fourteen kinds of spices, were investigated by Ames test. The antimutagenic effects against a direct mutagen, 2-nitrofluorene(2-NF), and two indirect mutagens, 2-anthramine(2-AT) and 2-acetamidofluorene (2-AF) in the S. typhimurium TA98 were tested. For the 2-NF, the antimutagenicity of cinnamon, feungreek, fennel, ginger, clove, turmeric and celery seed were determined as 42, 38, 32, 28, 24, 23 and 20%, respectively. The antimutagenicity of clove against the 2-AT was the highest (116%), and followed by the order of celery seed (103%), cardamon (100%), red pepper (99%), cinnamon(92%), cumin(83%), ginger(82%), fennel(82%), coriander (71%), nutmeg(68%) and turmeric (55%). The results also showed that the antimutagenic effect of clove against the 2-AF was superior to other spices. In case of curry powder among more than 10 kinds of spices, the antimutagenenicity against the 2-AT and 2-AF showed 23% and 6%, respectively, but no effect was observed against the 2-NF.

음식물 폐기물을 이용한 황토볼 제조시 적정 혼합비 연구

정승현,정병곤 한국폐기물자원순환학회 2015 한국폐기물자원순환학회지 Vol.32 No.8

Experiments were conducted to propose manufacturing methods to make loess ball using food wastes. Food wastes were used to improve porosity and increase specific surface area of loess ball. Red clay, food wastes and clay were used as main raw materials in making loess ball and these were mixed with varying ratio in order to find out optimum mixing ratio. The optimum mixing ratio of food wastes is evaluated to amount to 30%. In this case, 33.61 kg/㎠ of compressive strength, 65.13% of porosity, 50.04% of absorbing ratio, and 6.302 ㎡/g of specific surface area are obtained. When evaluating cross section and the appearance of red clay ball made up of 30% food wastes through a visual inspection and SEM photograph, it can be observed that a lot of pores are formed across the red clay ball due to the volatilization of moisture and organic matter in food wastes during the high-temperature burning. Taking into account compressive strength, porosity, absorbing ratio, specific surface area, and SEM photograph altogether, the optimum mixing ratio of loess, food wastes, and clay in the loess ball were 30%, 50%, and 20%, respectively. When evaluating the cross-section and outer appearance of the loess ball that comprise 30% of food wastes by SEM photography, it can be concluded that the optimum mixing ratio of loess, food wastes, and clay in the loess ball amounts to 30%, 50%, and 20%, respectively. Compressive strength of the manufactured loess ball amounts to 42.52 kg/㎠, which is 26.5% higher than the values obtained in the condition when the loess ball made without food wastes. However, the values of porosity, absorbing ratio, and specific surface area are somewhat lower than the corresponding values obtained in the condition when the loess ball was manufactured without food wastes.

축복인가, 찬미인가

정승현 광주가톨릭대학교 신학연구소 2010 神學展望 Vol.- No.170

六一順氣湯 抽出物이 생쥐의 全腦虛血에 미치는 影響

정승현,신길조,이원철 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.7 No.2

생쥐의 全腦虛血 모델에서 potassium cyanide 誘發 昏睡時間 및 生存時間, 減壓性 無酸素 負荷時 生存時間을 測定하여 全腦虛血時 腦循環代謝 改善 效果를 觀察하였다. KCN 誘發 昏睡時間의 短縮, 致死量의 KCN에 대한 生存時間의 延長, 減壓에 의한 無酸素 負荷時 生存時間의 延長 效果가 나타났다. The effect of Yukilsunki-tang extracts on global cerebral ischemia were investigated in this study. The multiple parameters of global cerebral ischemia assessed in mice included the duration of KCN-induced(1.8㎎/㎏ i.v.) coma, the survival time of KCN-induced(3.0㎎/㎏ i,v.) coma the survival time exposed to hypoxia induced by vacuum pump. In the case of global cerebral ischemia International Cancer Research mice were used and divided into two groups at random. Group A, normal control, was treated after oral administration of normal saline. Group B, experimental control, was treated after oral administration of 13.2㎎/20g of Yukilsunki-tang extracts. Each treatment was KCN-induced(1.8㎎/㎏ i.v.) coma, KCN-induced(3.0㎎/㎏ i.v.) coma and exposure to hypoxia induced by vacuum pump. The results were obtained as follows : In global cerebral ischemia, Yukilsunki-tang extracts significantly prolonged the duration of KCN-induced(1.8㎎/㎏ i.v.) coma, the survival time of KCN-induced(3.0㎎/㎏ i.v.) coma and the survival time of exposure to hypoxia induced by vacuum pump in mice. Conclusion Yukilsunki-tang extracts had a significant effect on Global cerebral ischemia.

Integrated analysis of copy number alteration and RNA expression profiles of cancer using a high-resolution whole-genome oligonucleotide array

정승현,Seung-Hun Shin,임선희,Hye-Sun Choi,이석형,정연준 생화학분자생물학회 2009 Experimental and molecular medicine Vol.41 No.7

Recently, microarray-based comparative genomic hybridization (array-CGH) has emerged as a very efficient technology with higher resolution for the genome-wide identification of copy number alterations (CNA). Although CNAs are thought to affect gene expression, there is no platform currently available for the integrated CNA-expression analysis. To achieve high-resolution copy number analysis integrated with expression profiles, we established human 30k oligoarray-based genome- wide copy number analysis system and explored the applicability of this system for integrated genome and transcriptome analysis using MDA-MB-231 cell line. We compared the CNAs detected by the oligoarray with those detected by the 3k BAC array for validation. The oligoarray identified the single copy difference more accurately and sensitively than the BAC array. Seventeen CNAs detected by both platforms in MDA-MB-231 such as gains of 5p15.33-13.1, 8q11.22-8q21.13, 17p11.2, and losses of 1p32.3, 8p23.3-8p11.21, and 9p21 were consistently identified in previous studies on breast cancer. There were 122 other small CNAs (mean size 1.79 mb) that were detected by oligoarray only, not by BAC-array. We performed genomic qPCR targeting 7 CNA regions, detected by oligoarray only, and one non-CNA region to validate the oligoarray CNA detection. All qPCR results were consistent with the oligoarray-CGH results. When we explored the possibility of combined interpretation of both DNA copy number and RNA expression profiles, mean DNA copy number and RNA expression levels showed a significant correlation. In conclusion, this 30k oligoarray- CGH system can be a reasonable choice for analyzing whole genome CNAs and RNA expression profiles at a lower cost.

Water Quality and Cyanobacterial Anatoxin-a Concentration in Daechung Reservoir

정승현,김지환,안치용,최신석,김희식,오희목 한국하천호수학회 2002 생태와 환경 Vol.35 No.5

하나님의 선교와 창조신학의 관점에서 본 ‘은총의 숲’ 사역

정승현 한국선교신학회 2022 선교신학 Vol.67 No.-

This paper examines the ‘Grove of Grace’ project in Mongolia, which is actively participating in the preservation and restoration of creation from the perspective of God’s mission and seeks its missiological implications. Since the IMC at Willingen in 1952, the notion of missio Dei has been developed in direct connection with ecclesiology rather than the Triune God itself. In the case of Korea, it did not provide an appropriate answer to the issue of materialism and other religions but focused on the soteriology of Jesus Christ. The Grove of Grace ministry, which started in Mongolia in 2009, is to prevent desertification in Mongolia due to global warming, to provides economic help to the Mongolians, and more than anything else to present a new mission plan for Korean Christianity. The Grove of Grace is practicing the intrinsic ‘fellowship’ of the Triune God with local people and creatures through ecological mission. 본고는 창조세계의 보존과 회복에 적극적으로 참여하고 있는 몽골 ‘은총의 숲’ 사업을 하나님의 선교 관점에서 검토하고 그 선교학적인 함의를 모색하고자 한다. 1952년 빌링겐IMC 이후 하나님의 선교(missio Dei)에 대한 다양한 논의들이 있었는데, 하나님의 선교가 삼위일체 하나님 자체보다는 교회론과 직접적으로 연관되어 발전되어 왔고, 한국의 경우 물질주의와 타종교 이슈에 대한 적절한 답변을 제시하지 못했으며, 예수 그리스도의 구원론에 집중하면서 창조론에 대한 논의가 미약했다는 점이 제기되었다. 2009년 몽골에서 시작된 ‘은총의 숲’ 사역은 지구 온난화로 인한 몽골의 사막화를 방지하고, 몽골인에게 경제적인 도움을 주며, 무엇보다도 한국기독교의 새로운 선교방안을 제시한다. 은총의 숲은 삼위일체 하나님의 내재적인 ‘사귐’을 현지인과 피조물과 함께 생태선교로 실천하고 있다.

메릴랜드주 교차로

정승현,이석홍,Jeong, Seung-Hyeon,Lee, Seok-Hong 한국도로학회 2001 도로포장공학회지 Vol.3 No.4