모기의 개체수를 효과적으로 감소하기 위한 방제 연구

이훈복,이명애,나수미,이희일,유재승,배연재 한국환경생태학회 2019 한국환경생태학회 학술대회지 Vol.2019 No.1

[特輯]신경회로망의 VLSI 구현과 뉴로컴퓨터

이훈복,최명렬 한국정보과학회 1992 정보과학회지 Vol.10 No.2

전수조사를 통한 국내 유전자변형생물체의 연구 동향 분석

이훈복,최경화,정순기,김용호,김환묵 한국식물생명공학회 2008 식물생명공학회지 Vol.35 No.1

We analyzed the current research trends of living modified organisms (LMO) by questionnaires in the interest of making biosaftey laws and policies in Korea. We executed a pre-survey at the Crop Functional Genomics 2004 conference and obtained LMO research information from 423 LMO research organizations, including 32 national research institutes, 314 universities, and 77 industries. We found that the total 59 kinds of hosts including 26 kinds of plants, 15 kinds of animals, and 18 kinds of microbes were used for LMO research and E. coli was the most common host. The risk of the most experimental hosts was below a biosafety level of 1 (73.8%) and 2 (25.9%). LMO development use purpose was implemented in various developmental uses: 51.3% in test and research use, 19% in health and medical use, and 12.9% in agriculture use. The experiment product, waste product, and products of host for LMO development were 327.2, 223.6, and 13.5 in number of plants; 280.6, 52.4, and 8.7 in number of animals; and 8.3 x 1011CFU, 7.7 x 1011CFU, and 6.5 x 1011CFU in microbes in 2004. The survey results about how to possess the LMO were very unreliable, because only 10.6% of the researchers returned the questionnaires. Consequently, we strongly suggest the scientific organizations as well as scientists should have more interests in biosafety of LMO research and an LMO biosafety management system should be developed for Korea’s future biotechnology.

Effects of global warming on mosquitoes & mosquito‐borne diseases and the new strategies for mosquito control

이훈복,Bijaya R. Devkota,유재승,오기철,김진홍,김현정 한국곤충학회 2014 Entomological Research Vol.44 No.6

Global warming has shortened mosquitoes’ lifecycle period and increased the disease transmission rates by mosquito vectors. We reviewed only three mosquito‐borne diseases: malaria, dengue fever, and the Japanese encephalitis. Billions of people get infected with those diseases and millions of people die every year. Although we struggle to find the most effective way to control mosquitoes using various methods (including pesticides), mosquito‐borne diseases are still among the most serious problems being faced. This paper, therefore, reviews the strategies for controlling mosquitoes. The use of pesticides to control mosquitoes might have more negative effects on humans and environments than benefits. Although the development of genetically modified (GM) mosquitoes raises new hopes for effective mosquito control, it will take longer to assess the risks to humans and environments. Furthermore, there has been concern about the possible adverse effects from the release of GM mosquitoes into the environment. The various mosquito traps may not be as effective at controlling only female mosquito populations. Therefore, new strategies for the control of mosquitoes are vital. The smart mosquito counter device was developed by Korean Centre for Disease Control (KCDC) in 2013. The mosquito pest control office is able to set up the appropriate mosquito control strategies by using quantitative mosquito information. The smart device will bring mosquito control in line with modern smart generation technology and the device will also soon be able to identify different mosquito species. This new strategy will change the methods of mosquito control and will provide beneficial effects toward sustainable nature and human health.

k8048 을 이용한 음성 합성 시스템 ( Application of K8048 in Speech Synthesis System )

이훈복 대한전자공학회 1983 대한전자공학회 학술대회 Vol.1983 No.1

Environment Risk Assessment of Watermelon Grafed onto Transgenic Rootstock resistant to Cucumber Green Mottle Mosaic Virus (CGMMV) on Non-Target Insects in Conventional Agro-Ecosystem

이훈복,박지은,권민철,박상규,김창기,정순천,윤원기,박상미,한상렬,함치학,김환묵 한국생태학회 2006 Journal of Ecology and Environment Vol.29 No.4

We investigated the impact of watermelon grafted onto Cucumber Gren Mottle Mosaic Virus(CGMMV)-resistant transgenic watermelon rotstock on insects as non-target organisms in a greenhouse in2005. We quantitatively collected insect assemblages living on leaves and flowers, and we used sticky traps to(Aphis gossypii Glover) on watermelon leaves and western flower thrip (Frankliniella occidentalisTrybom) onwatermelon male flowers, betwen CGMMV-resistant transgenic watermelon (TR) and non-transgenic water-melon (nTR). Non-parametric multidimensional scaling (NMS) ordination verified that insect assemblages onleaves and sticky traps were different between TR and nTR (P < 0.05). The insect assemblages on male flowerswere not statistically significant. Multi-response permutation procedures proofed our esults from NMS results (P> on leaves and sticky traps, but watermelon male flowers do not show an adverse effect. Further research isrequired to assess the ffect of TR on the aphid and western flower thrip. Life table experiments might supportthe specific reason for the adverse effects from leaf assemblages. Assessment of non-target impacts is anessential part of the risk assessment of non-target insects for the impact of transgenic organisms.

입원 기간 중 발생한 첫 경련발작

이훈복,김종흥,김제,이애영,김재문 대한신경과학회 1997 대한신경과학회지 Vol.15 No.2

Effects of transgenic watermelon with CGMMV resistance on the diversity of soil microbial communities using PLFA

이훈복,김창기 한국통합생물학회 2010 Animal cells and systems Vol.14 No.3

We compared the composition of phospholipid fatty acids (PLFA) to assess the microbial community structure in the soil and rhizosphere community of non-transgenic watermelons and transgenic watermelons in Miryang farmlands in Korea during the spring and summer of 2005. The PLFA data were seasonally examined for the number of PLFA to determine whether there is any difference in the microbial community in soils from two types of watermelons, nontransgenic and transgenic. We identified 78 PLFAs from the rhizosphere samples of the two types of watermelons. We found eight different PLFAs for the type of plants and sixteen PLFAs for the interaction of plant type and season. The PLFA data were analyzed by analysis of variance separated by plant type (PB0.0085), season (PB0.0154), and the plant typeseason interaction (PB0.1595). Non-parametric multidimensional scaling (NMS)showed a small apparent difference but multi-response permutation procedures (MRPP) confirmed that there was no difference in microbial community structure for soils of both plant types. Conclusively, there was no significant adverse effect of transgenic watermelon on bacterial and fungal relative abundance as measured by PLFA. We could reject our hypothesis that there might be an adverse effect from transgenic watermelon with our statistical results. Therefore, we can suggest the use of this PLFA methodology to examine the adverse effects of transgenic plants on the soil microbial community.

국가 장기 생태 연구지(남산, 지리산, 월악산)의 신갈나무림과 소나무림에서 포획된 나방류의 종조성 및 종다양도

이훈복,김현정,Yi, Hoon-Bok,Kim, Hyun-Jung 한국응용곤충학회 2010 한국응용곤충학회지 Vol.49 No.2

본 연구는 2005년과 2006년 6월부터 9월까지 남산, 지리산, 월악산의 신갈나무림, 소나무림에서 유브이 버킷 라이트 트랩(22 W)을 이용하여 나방을 포획하였고 식생 및 삼림의 주요 1차 소비자인 나방류(불나방과, 자나방과, 밤나방과, 재주나방과, 명나방과)의 종다양성을 시공간적으로 비교하였다. 본 연구기간동안 국가장기생태연구지에서 포획된 나방의 종수는 435종이었다. 포획된 나방의 개체수는 월악산에서 가장 높았고, 지리산, 남산 순이었다. ANOVA 분석결과 개체수를 종속변수로 하였을때는 조사시기에 대해 유의한 차이가 나타났고 종풍부도의 경우에는 조사시기와 지역에 따른 유의한 차이가 나타났다. 다변량분석 방법인 다차원분석법을 사용하였을때, 나방의 군집구조는 각 지역별로 뚜렷이 구분되어 나타났지만, 같은 지역내의 산림유형에 따른 구분은 명확하지 않았다. Moths were collected using a 22W UV black light trap in June through September in 2005 and May, June, August and September in 2006. The study sites were Namsan (Mt. Nam), Jirisan (Mt. Jiri), Woraksan (Mt. Worak) which are part of the Korean National Long Term Ecological Research (KNLTER). There were two common forest communities, Quercus mongolica and Pinus densiflora. The purpose of this study was to compare species diversity of the major plant feeding Lepidoptera in the two forest types at the regional KNLTER sites. We collected a total of 435 species from the KLTER sites in 2005 and 2006. Abundance of moths was highest at Woraksan (Mt. Worak) followed by Jirisan (Mt. Jiri). The Namsan (Mt. Nam) site had the lowest, with five families (Arctiidae, Geometridae, Noctuidae, Notodontidae, and Pyralidae). Analysis of variance (ANOVA) showed statistically significant differences at sampling date in species abundance as a response variable and at site in species richness as another response variable. Although we expected a distinct cluster with the forest type at each study site, one of ordination analyses, nonmetric multidimensional scaling (NMS), showed distinct clusters with the moth assemblages at each site only but NMS did not show any distinct cluster with the different forest types at each site as we expected.

LMO 격리 포장에서 유전자비변형 모본 고추(P915)와 유전자 변형 고추(CMVP0-CP)에 서식하는 절지동물 군집 비교연구

이훈복,김현정,Yi, Hoon-Bok,Kim, Hyun-Jung 한국응용곤충학회 2010 한국응용곤충학회지 Vol.49 No.1

토마토나 고추 등의 유용작물에 자주 발생하여 생산량을 저하시키는 오이모자이크병에 대한 저항성을 높여 생산량을 증대시키기 위하여 개발된 CMVP0-CP (Cucumber mosaic virus-coat protein) gene이 삽입된 유전자 변형 CMVP0-CP 고추 (line 7)에 대한 환경위해성을 평가하였다. 2007년 고추의 생육기간 동안 절지동물의 군집구조를 3회(6월 19일, 7월30일, 8월 31일)에 걸쳐 조사를 하였다. 두 가시의 고추, 즉 모본(P915, nTR)고추와 유전자변형 고추(CMVP0-CP (line 7), TR)의 꽃과 잎에 서식하는 곤충을 포함한 절지동물의 군집구조를 파악하기 위하여 곤충을 포획할 수 있는 곤충진공포획기를 사용하여 절지동물을 정량적으로 채집하였다. 절지동물의 군집은 채집 시기별로 출현 종과 빈도수에 차이는 있었지만 두 작물간의 군집 구조는 통계학적으로 차이를 나타내지 않았다. 지금까지 수행된 본 연구결과를 근거로 판단하여 볼 때, 유전자 변형 고추로 인한 비표적 생물체의 군집구조가 모본 고추의 군집구조와 차이가 나타나지 않아 환경 위해서도 없는 것으로 볼 수 있지만, 좀더 확실한 유전자변형생물체인 고추의 환경위해성이 없다는 결론을 얻기 위해서는 추가적인 연구가 수행되어져야 할 것이다. The environmental risks of cucumber mosaic virus resistant transgenic chili peppers with the CMVP0-CP gene on non-target organisms in the agroecosystem environments was evaluated during the periods of the chili pepper growing season (June 19, July 30, August 31) in 2007. Arthropods assemblages leaves and flowers of chili peppers were quantitatively collected by using an insect vacuum collector to compare the arthropod community structures between non-transgenic chili peppers (nTR, P 915) and mosaic virus resistant transgenic chili peppers (TR, CMV-cp, line 7). There was no statistical difference in the arthropod community structure between the two types of crops, nTR and TR, at the same season, although the species richness and Shannon's index were somewhat different among seasons; indicating no effects of genetically modified peppers on the arthropod community. However, further studies were required to conclude more concretely for the potential environmental risk of the transgenic chili pepper of CMV-cp.