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이형상 칼슘 인산염(Biphasic Calcium Phosphate)에 의한 성체 줄기세포의 골아세포 분화
이명구,임성우,피성희,김윤상,신형식,유형근,Lee, Myoung-Ku,Lim, Sung-Woo,Pi, Sung-Hee,Kim, Yun-Sang,Shin, Hyung-Shik,You, Hyoung-Keun 대한치주과학회 2005 Journal of Periodontal & Implant Science Vol.35 No.4
The present study was to determine the influence of micro-macro biphasic calcium phosphate(MBCP) on proliferation and differentiation of human marrow-derived mesenchymal stem cells. Primary stem cells were cultured from bone marrow and 3-4 passaged cells were used. This study tested the proliferative effects by cell counting. Collagen sythensis, alkaline phosphatase activity, expression of osteocalcin and bone sialoprotein by Western blot analysis were evaluated. The cellular proliferation of ASC was not influenced by MBCP. Collagen synthesis of ASC cultured on MBCP significantly increased at 5th and 7th days(p<0.05). The ALP activity in ASC cultured on MBCP significantly increased at 5th and 7th days(p<0.05). The expression of OC and BSP incresaed in ASC cultured on MBCP. These results suggest that MBCP may stimulates the osteoblastic activity of ASC.
수종의 생약제제가 human fetal osteoblasts의 염기성 인산분해 효소 활성에 미치는 영향
이명구,최희인,유형근,신형식,Lee, Myoung-Ku,Choi, Hee-In,You, Hyung-Keun,Shin, Hyung-Shik 대한치주과학회 2003 Journal of Periodontal & Implant Science Vol.33 No.1
Several growth factors and polypeptides are not commonly yet used for regenerators of bone tissue or alveolar bone because of the insufficiency of studies on their side effects, genetic engineering for mass production and stability for clinical application. Recently, many herbal medicines, which have advantage of less side effects and possibility of long-term use, have been studied for their capacity and effects of anti-bacterial, antiinflammatory and regenerative potential of periodontal tissues. Morindae Radix, Cibotium Barometz (L.), Albizziae Cortex, Cistandhis Herba have been traditionally used as medicines for treatment of bone disease in Eastern medicine. The objective of the present study is to examine the ability of alkaline phosphatase (ALP) activity of human fetal osteoblast (hFOB1) when several natural medicines were supplemented. hFOB1 were cultured with Dulbecuo's Modified Eagle's Medium Nutrient Mixture F-12 HAM ( DMEM/F-12 1:1 Mixture, Sigma, USA) and negative control, dexamethasone (positive control), and each natural medicines for 3 days. And then ALP activity was measured by spectrophotometer for enzyme activity and Alizarin red S staining for morphometry. Among the natural medicines of this study, Morindae Radix, Cibotium Barometz (L.) and Cistanchis Herba induced higher activity of ALP synthesis than negative controls in all experimental group. Albizziae Cortex showed mild increases than negative control group. According to measurement of positively stained area, all of the natural medicines of this study increased compared to negative control. Especially, Cibotium Barometz (L.) and Cistanchis Herba showed statistical significance compared to negative control (p<0.05). These results indicate that Morindae Radix, Cibotium Barometz (L.), Albizziae Cortex, Cistandhis Herba have an inducing ability of ALP synthesis on osteoblast.
펄프ㆍ제지용 원료로서의 삼 섬유 이용에 관한 연구(제2보)
이명구(Myoung-Ku Lee),김지섭(Ji-Seop Kim),윤승락(Seung-Lak Yoon) 한국펄프.종이工學會 2011 펄프.종이기술 Vol.43 No.5
Hemp bast pulp cooked at temperature below 100℃ followed by defibration by the knife and the valley beater, respectively was mixed with softwood pulp varying the amount of hemp pulp in order to find the optimum condition for making hemp-wood paper. Both the knife and the valley beaters contributed to the dispersion of pulp fiber well. Lots of shives were found when the knife beater was applied exclusively, but the fibers were dispersed well when freeness dropped to 600 mL CSF and 500 mL CSF by the valley beater. Air resistance decreased drastically below 500 mL CSF where rapid disrupture of pulp fiber occurred. As the values for freeness and hemp fiber content increased, so did roughness and bulk. It was apparent that the tear strength of hemp-wood paper was on the rise drastically as hemp fiber content increased. Nevertheless the optimum hemp fiber content of hemp-wood paper would be 20% considering the decrease in both tensile and burst strengths as well as sheet formation.
Effects of biphasic calcium phosphate on bone formation in human fetal osteoblasts
신계철,장길용,이명구,윤호상,송제봉,김현아,피성희,신형식,유형근,Shin, Kye-Chul,Jang, Kil-Young,Lee, Myoung-Ku,Yoon, Ho-Sang,Song, Jae-Bong,Kim, Hyun-A,Pi, Sung-Hee,Shin, Hyung-Shik,You, Hyung-Keun The Korean Academy of Periodontoloy 2005 Journal of Periodontal & Implant Science Vol.35 No.1
목적 : 이 연구의 목적은 치과 영역에서 골 재생을 촉진하기 위해, 현재 많이 사용하고 있는 BDX(bovinederived xenograft)와 비교하여 BCP(biphasic calcium phosphate)의 효과를 알아보기 위함이다. 실험 재료 및 방법: 본 연구는 태아골모세포주(hFOB 1.19)를 사용하였으며, 사용된 골 이식재에 따라 2개의 실험군으로 구분하였고, 각 실험에 적절한 농도의 BDX와 BCP를 첨가하였다. 그리고, 세포 증식도 검사, 교원질 합성량 분석, 염기성 인산분해효소 활성도 측정, Western blot 분석을 통한 OC과 BSP의 발현 정도등의 실험을 진행하였다. 결과 : BDX와 BCP는 대조군과 비교하여 세포 증식에서 유의한 차이가 없었지만, 교원질 합성량, 염기성 인산분해효소의 활성, 그리고 OC과 BSP의 발현에 있어 대조군과 비교하여 유의하게 증가를 보였다. 그러나, 두 이식재간의 유의한 차이는 보이지 않았다. 결론 : 본 실험실적 연구에서 BCP는 골모세포분화에 긍정적인 영향을 미침으로써 효과적인 이식재로 사용할 수 있음을 가늠할수 있었다.