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Electroporation 에 의한 담배 세포로의 유전자 도입
이광웅,이종섭,서정원 한국유전학회 1989 Genes & Genomics Vol.11 No.2
In order to improve the method of plant transformation, transfer of DNA into intact cells of tobacco was carried out by electroporation. Chimaeric genes between tomato proteinase inhibitor I and chloramphenicol acetyltransferase genes were constructed in a vector derived from Ti-plasmid of Agrobacterium tumefaciens. Suspension cells of tobacco were electric-shocked in a chamber with DNAs of one of the constructs, pJSL105 and were plated on culture medium containing kanamycin. When shocked at 4㎸/㎝, colonies were formed from the cells on the medium containing 200㎍/ml kanamycin. The kanamycin-resistant colonies were further cultured to form calluses, which were regenerated into shoots. Roots were initiated from the shoots derived from kanamycin-resistant calluses while not from control. Southern hybridization of genomic DNAs isolated from leaves of a transgenic plant revealed the presence of the plasmid DNA. This indicated that intact cells can be used as plant material for the gene transfer by electroporation.
Banti 씨 증후군의 (症候群) 간 (肝 ) Scintigram 에 관한 연구
이광웅,류방현 ( Kwang Ung Lee,Bang Hyun Liu ) 대한소화기학회 1979 대한소화기학회지 Vol.11 No.1
The pnrpose of this study was to evaluate the diagnostic usefulness of liver scanning with radiogold in cases of Bantis syndrome. Patients with Bantis syn3rome were admitted to the Department of internal Medicine at the Busan '.Vational Universitiy Hospital from Jan. 19ol to Dec. 1977 or studies. L.iver scanning ivith ra(lioactive colloid1 gold was carried out in 45 cases of Banti's syndrome. Correlation between scintigraphic lindings and chemical liver function studies including clinical fe:tnres ivere also observed. In patients with Banti's syndrome, hypertrophy pvttern of the ]iver ivas found in 14 cases (31.1.,), atrophy pattern of the liver in 20 cases (44.4,) and normal size in 11 cases (2-l. Yqr) Mottling was not significant in cases with hypertrophy and less significant even in patients of atrophy pattern. In patients having marked spleen visualization, the spleen is enlarged more than the size of the liver on scintigram. Che!niczl lievr function studies show ivithin normal limits in cases of hypertrophy pst.ern of the liver. The spleen was visualized in all of the patients (100.), moderate visualization in 15 cses (33.3,4) and marked visualization in 30 cases (66.7g). The author found atrophy pattern of the liver and marked visualization of the spleen in the scintigram of the advanced cases of Bantis syndrome, which suggested diagnostic usefulness of radiogo!d scanning of Bantis syndrome.
이광웅 대한간학회 2010 Clinical and Molecular Hepatology(대한간학회지) Vol.16 No.4(S)
HCV infection becomes an important indication for liver transplantation in Korea. Outcome of HCV-associated liver transplantation has been worse than transplantation from other causes. This is mostly related to high rate of HCV recurrence lea thanto graft failure. Current antiviral therapies are inadequate and ineffective in the v Th majority of patients. However, a sustained virologic response is associated with improved survival. Strategies for minimizthanthe frequency of severe HCV recurrence worse e avoidance of ntatiodonors, ear, ye faanosis/treatment of CMV and minimization of immunosuppression (avoidance of corticoid pulse therapy). Antiviral therapy prior to significant damaae to the allograft is Thisf sy recommended. Psurvivalshould be offered treatment with peg-interferon and ribavirin as soon as histological evidence of recurrence of HCV is apparent after liver transplantation.