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윤보애,양은미,김찬종 대한진단검사의학회 2018 Annals of Laboratory Medicine Vol.38 No.5
Background: Urinary tract infection (UTI) is the most common bacterial infection in infants. Renal parenchymal involvement is an important prognostic factor; however, early detection of parenchymal involvement in UTI may be difficult during infancy. This study aimed to assess whether a recently established biomarker of UTI, neutrophil gelatinase-associated lipocalin (NGAL), can serve as a useful marker for the detection of cortical defects (CD) and to determine the appropriate diagnostic cut-off value of NGAL in infants with febrile UTI. Methods: Infants hospitalized for febrile UTI were divided into two groups according to the presence of cortical defects on dimercaptosuccinic acid (DMSA) scintigraphy. Among 64 enrolled infants, 43 (67%) had CD (UTI-CD) and 21 (33%) had no CD (UTI-ND). The white blood cell count, C-reactive protein, and plasma NGAL (pNGAL) levels were determined before antibiotic therapy and compared between the two groups. Results: pNGAL level was significantly higher in the UTI-CD group than in the UTI-ND group (340 μg/L vs 214 μg/L, P=0.002). Multivariate analysis showed that pNGAL level was the only independent predictor of CD (odds ratio 2.759, P=0.039). In the ROC curve analysis, pNGAL showed the highest area under the curve (0.745; 95% confidence interval, 0.561–0.821; P=0.014). The appropriate cut-off value of pNGAL was 267 μg/L (sensitivity, 72.1%; specificity, 71.4%). Conclusions: pNGAL was found to be a useful marker for early prediction of renal parenchymal involvement in infants with febrile UTI.
인간 배아줄기세포의 feeder-free 배양 환경에 사용된 다양한 기질 고찰
윤보애 ( Bo Ae Yoon ),문신용 ( Shin Yong Moon ),최영민 ( Young Min Choi ) 서울대학교 인구의학연구소 2010 人口醫學硏究論集 Vol.23 No.-
Human embryonic stem cells(hESCs) have been derived and cultured in various condition. Culture of undifferentiated hESCs traditionally requires mouse fibroblasts as a feeder cell layer. The hESC culture using mouse fibroblasts makes clinical application of the hESCs difficult because that the mouse fibroblasts could give a risk of contamination with xeno-pathogens. Therefore, replacing feeder cells with defined human extracellular matrix(ECM) components or synthetic biomaterials would be ideal for the maintenance of clinical-grade hESCs. In this review, we discuss with recent advances in the use and development of substrates suitable for feeder-free culture condition.
윤보애 ( Bo Ae Yoon ),오선경 ( Sun Kyung Oh ),최영민 ( Young Min Choi ) 서울대학교 인구의학연구소 2013 人口醫學硏究論集 Vol.26 No.-
Human embryonic stem cells (hRSCs) have unlimited potentials of proliferation and differentiation and these properties of hESCs make them considered as most interesting cell types for tissue engineering, cell therapy, and drug screening. Stem cell bank mainly do quality control of stem cell lines including best practice in culture, preservation and provide information for the choice of proper embryonic stem cell (ESC) line to proceed further studies. Currently, USA and UK are leading counties for stem cell research and banking. This paper reviews, the current state of the USA and the UK stem cell bank and compared the differences between two banks.
인간 배아줄기세포 배양에서 마이코플라즈마 오염 시 제거를 위한 연구
윤보애 ( Bo Ae Yoo ),백진아 ( Jin Ah Baek ),설혜원 ( Hye Won Seol ),정주원 ( Ju Won Jung ),오선경 ( Sun Kyung Oh ),최영민 ( Young Min Choi ) 서울대학교 인구의학연구소 2014 人口醫學硏究論集 Vol.27 No.-
Mycoplasma is one of the smallest and simplest prokaryotes. Mycoplasma contamination during cell culture is widespread, and the use of contaminated cells compromises almost all aspects of cell physiology and consequently the results and conclusions from numerous experiments. In this study, we tried to eliminate the mtcoplasma from mtcoplasma contaminated cases during human embryonic stem cell(hESC) culture. hESC lines (SNUhES4, SNUhE31, H1) were cultured with three diffetent antimycoplasma antibiotics (Moxifloxacin+Gentamicin, Plasmocin, Plasmocure) for 4~5 passges, We observed morphologies, rete of attachment and differentiation of hESCs. Also, we confirmed relative treatment efficiency (eliminated, resistant, dead) of antimtcoplasma antibiotics. Three weeks after Plasmocure treatment, high attachment tate and mycoplasma free condition were well maintained. Relative treatment efficiency of Plasmocure showed eliminated 88.9%, resisrant 11.1%, dead 0%. Among the tested antimycoplasma antibiotics, Plasmocure was a most effective antimycoplasma antibiotic to eliminate mycoplasma from contaminated cell culture cases.
세포 배양 시 마이코플라즈마의 검출 및 제거 방법에 대한 연구 동향
윤보애 ( Bo Ae Yoon ),오선경 ( Sun Kyung Oh ),최영민 ( Young Min Choi ) 서울대학교 인구의학연구소 2011 人口醫學硏究論集 Vol.24 No.-
Mycoplasma contamination may have multiple effects on cultured cells and can also have significant influences on the results of scientific studies. Therefore, the elimination of mycoplasma contamination from cell cultures is necessary. This review provides an overview of the following research trends: (1) mycoplasma contamination in cell cultures; (2) various techniques available for the detection of mycoplasma; (3) four method types available for the elimination of mycoplasma We will discuss the problems of mycoplasma contamination in cell cultures and present a concise overview on the biology of mycoplasma with suggestions on efficient methods for its detection and elimination, and proposing steps to be taken to prevent the problems.
윤보애 ( Bo Ae Yoon ),설혜원 ( Hye Won Seal ),최영민 ( Young Min Choi ) 서울대학교 인구의학연구소 2012 人口醫學硏究論集 Vol.25 No.-
Human embryonic stem cells (hESCs) are one of the most interesting cell types for tissue engineering, cell therapy, basic scientific research, and drug screening. Fast advancement in these areas requires the availability of large amounts of safe and well-characterized hESCs from hESC banks. The sensitivity of hESCs to cryopreservation, however, has severely limited the utility of these cells. It was observed that the characteristics of hESCs changed during long-term culture. Therefore, it is crucial to preserve early-passage cells by effective freezing and thawing methods. This paper reviews the current state of cryopreservation as it pertains to hESCs. We focused on the research of cryopreservarion method of hESCs.
소윤희,김대성,윤보애,최유덕,백희조,국훈 대한소아혈액종양학회 2019 Clinical Pediatric Hematology-Oncology Vol.26 No.2
Hepatic osteodystrophy is frequent complication in patients with chronic liver disease, particularly with chronic cholestasis. We report a male infant with congenital hepatoblastoma, who had osteodystrophy complicated by multiple bone fractures despite adequate supplementation of fat-soluble vitamins including vitamin D. He was born by Caesarean section because of a 7 cm–sized abdominal mass detected by prenatal ultrasonography. The pathologic diagnosis was hepatoblastoma, PRETEXT staging III or IV. Whole body bone scan at the time of diagnosis showed no abnormal uptake. Oral vitamin D3 of 2,000 IU/day was administered with other fat-soluble vitamins. Serum direct bilirubin level gradually increased up to 28.9 mg/dL at postnatal 6 days and was above 5 mg/dL until 110 days of age. Bony changes consistent with rickets became apparent in left proximal humerus since 48 days of age, and multiple bone fractures developed thereafter. With resolving cholestasis by chemotherapy, his bony lesions improved gradually after add-on treatment of bisphosphonate and parenteral administration of vitamin D with calcium. High level of suspicion and prevention of osteodystrophy is needed in patients with hepatoblastoma, especially when cholestasis persists.
The First Korean Family with Hemoglobin-M Milwaukee-2 Leading to Hereditary Methemoglobinemia
김대성,백희조,김보람,윤보애,이준형,국훈 연세대학교의과대학 2020 Yonsei medical journal Vol.61 No.12
Hemoglobin M (HbM) is a group of abnormal hemoglobin variants that form methemoglobin, which leads to cyanosis and hemolyticanemia. HbM-Milwaukee-2 is a rare variant caused by the point mutation CAC>TAC on codon 93 of the hemoglobin subunitbeta (HBB) gene, resulting in the replacement of histidine by tyrosine. We here report the first Korean family with HbM-Milwaukee-2, whose diagnosis was confirmed by gene sequencing. A high index of suspicion for this rare Hb variant is necessary in apatient presenting with cyanosis since childhood, along with methemoglobinemia and a family history of cyanosis.
이현경(Hyun Gyung Lee),윤보애(Bo Ae Yoon),김영옥(Young Ok Kim),김명규(Myeong Kyu Kim),우영종(Young Jong Woo) 대한소아신경학회 2018 대한소아신경학회지 Vol.26 No.2
NCLs는 선천성 대사 이상 질환의 하나로, 리소좀에 자가 형광물질인 lipopigment가 축적되어 발생하는 신경퇴행성 질환이다. 2세에서 4세 사이에 발병하는 전형적인 영아 후기형 NCL에 비해 더 늦은 나이인 3세에서 8세 사이에 경련과 점진적인 시력 소실이 시작되는NCLs를 변이 영아 후기형 NCLs (Variant forms of late infantile NCLs,vLINCLs)이라고 하며, 이는 CLN6 에 의해 생긴다고 알려져 있다. 저자들은 진행성 근간대 뇌전증으로 진단된 여아에서 whole-exome sequencing을 통해서 CLN6 유전자 (NM_017882.2; NP_060352.1)의 c.296A>G(p.Lys99Arg)와 c.307C>T(p.Arg103Trp) 이형접합체 변이들을 발견하였고 이를 Sanger sequencing으로 확인하였다. 환아는 7세 이후에 여러 형태의 경련을 보였고, 저명한 광과민성 근간대 경련, 진행성 운동 및 언어능력 퇴행, 추체로 및 추체외로 징후, 뇌자기공명 검사에서 진행성 뇌위축 등을 vLINCLs의 전형적인 특징을 보였다. 본 증례는 한국인에서 첫번째로 보고되는 CLN6 유전자 변이에 의한 vLINCLs보고이며, 일본에서 보고된 증례와 같이 청소년기에도 시력 소실을 보이지 않았다. Neuronal ceroid lipofuscinoses (NCLs) are inherited neurodegenerative disorders, which are caused by the accumulation of lipopigment in lysosomes. Variant forms of late infantile NCLs (vLINCLs) characterized by a later onset of seizures and visual impairment (3–8 years) than in the classic form (2–4 years) are caused by mutations of the gene encoding ceroid lipofuscinosis neuronal protein 6 (CLN6). In a girl with progressive myoclonus epilepsy, we found heterozygous variants of CLN6 (NM_ 017882.2; NP_060352.1): c.296A>G (p.Lys99Arg) and c.307C>T (p.Arg103Trp). They were identified with whole-exome sequencing and verified with Sanger sequencing. At 7 years and 9 months, our patient had developed multiple types of seizures, prominent myoclonus with photosensitivity, regression in motor and language skills, pyramidal and extrapyramidal signs, and brain atrophy in brain images, all of which were progressive and were compatible with vLINCLs. However, this first Korean report shows no visual impairment, which resembles the previously reported Japanese case.