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연구노트 : 3T3-L1 지방세포내 ROS 생성에 대한 천년초 열수 및 에탄올 추출물의 항산화 효과
윤보라 ( Bo Ra Yoon ),이영준 ( Young Jun Lee ),김선구 ( Sun Gu Kim ),장중영 ( Jung Young Jang ),이효구 ( Hyo Ku Lee ),이성갑 ( Seong Kap Rhee ),홍희도 ( Hee Do Hong ),최현선 ( Hyeon Son Choi ),이부용 ( Bo Yong Lee ),이옥환 ( Ok Hw 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2012 한국식품저장유통학회지 Vol.19 No.3
Recently, NADPH oxidase 4 (NOX4)-mediated generation of intracellular reactive oxygen species (ROS) was proposed to accelerate adipogenesis of 3T3-L1 cell. We have previously shown that Cheonnyuncho (Opuntia humifusa) extract significantly inhibited adipocyte differentiation via downregulation of PPARγ (peroxisome proliferator-activated receptor gamma) gene expression. In this study, we focused on the molecular mechanism(s) of NOX4, G6PDH (glucose-6-phosphate dehydrogenase) and antioxidant enzymes in anti-oxidative activities of 3T3-L1 adipocytes. Our results indicate that Cheonnyuncho extracts markedly inhibits ROS production during adipogenesis in 3T3-L1 cells. Cheonnyuncho extracts suppressed the mRNA expression of the pro-oxidant enzyme such as NOX4 and the NADPH-producing G6PDH enzyme. In addition, treatment with Cheonnyuncho extract was found to upregulate mRNA levels of antioxidant enzymes such as Mn-SOD (manganese-superoxide dismutase), Cu/Zn-SOD (copper/zinc-SOD), glutathione peroxidase (GPx), glutathion reductase (GR), and catalase, all of which are important for endogenous antioxidant responses. These data suggest that Cheonnyuncho extract may be effective in preventing the rise of oxidative stress during adipocyte differentiation through mechanism(s) that involves direct down regulation of NOX4 and G6PDH gene expression or via upregulation of endogenous antioxidant responses.
쓴메밀 및 단메밀 에탄올 추출물의 항산화 및 지방세포 분화억제 효과
윤보라 ( Bo Ra Yoon ),조봉제 ( Bong Jae Cho ),이효구 ( Hyo Ku Lee ),김대중 ( Dae Jung Kim ),이성갑 ( Seong Kap Rhee ),홍희도 ( Hee Do Hong ),김경탁 ( Kyung Tack Kim ),조장원 ( Chang Won Cho ),최현선 ( Hyeon Son Choi ),이부용 ( Boo 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2012 한국식품저장유통학회지 Vol.19 No.1
In this study, 80% ethanolic extracts of tartary and common buckwheats were assessed for their total phenol content, total flavonoids content, antioxidant activity (DPPH, ABTS radical scavenging activity and reducing power), and anti-adipogenic effects. Our results show that total phenol contents of 80% ethanolic extract from tartary and common buckwheats were 17.35±0.41 and 8.20±0.28 μg GAE/g, respectively. Antioxidant activities of 80% ethanolic extract from tartary buckwheat were significantly higher than that of common buckwheat extract (p<0.05). During adipocyte differentiation, 80% ethanolic extracts of tartary and common buckwheat significantly inhibited lipid accumulation compared to control cells. We further evaluated the effect of buckwheat extracts on the changes of key gene expression associated with 3T3-L1 adipogenesis and ROS production. Tartary buckwheat extract was more suppressed the mRNA expressions (PPARγ and aP2) than that of common buckwheat extract. Moreover, tartary buckwheat inhibited the mRNA expression of both NOX4 (NADPH oxidase 4) and G6PDH (glucose-6-phosphate dehydrogenase). These results indicate that anti-adipogenesis effect of tartary buckwheat can be attributed to phenolic compound that may potentially inhibit ROS production.
열 태양광 발전을 위한 원형 홀 패턴의 Metal-Dielectric-Metal 선택형 방사체
윤보람(Boram Yoon),이남규(Namkyu Lee),배지열(Ji-Yeul Bae),Fakadu Tolessa,조형희(Hyung Hee Cho) 대한기계학회 2018 大韓機械學會論文集B Vol.42 No.5
본 연구에서는 열 태양광 발전을 위한 원형 홀 패턴의 Metal-Dielectric-Metal 선택형 방사체를 제안한다. 텅스텐과 산화 알루미늄을 구조체 전체적으로 사용하여 내열성을 확보하였다. 설계 변수의 변화에 따른 흡수/방사 특성을 분석하였고, 이를 통해 InGaAs 셀의 높은 변환 효율을 갖는 파장 대역에서 선택적으로 높은 방사율을 갖는 구조체를 형성하였다. 높은 흡수/방사의 파장 대역에서 텅스텐 원형 홀패턴의 가장자리 주변에서 국부적으로 발생하는 표면 플라즈몬 공명이 발생하였다. 이러한 공명은 루프형태의 유도 전류를 형성함으로써 자기장을 유전체 층에 가두는 효과가 있다. 본 연구에서 제안하는 선택형 방사체의 스펙트럼 효율은 1000K에서 41.0%, 1300K에서 63.7 %였다. 이는 동일한 온도의 흑체보다 각각 5배, 3배 높은 효율을 보였다. In this study, we propose a thermophotovoltaic circular hole pattern Metal-Dielectric-Metal selective emitter. Tungsten and aluminum oxides are used to ensure heat resistance. By analyzing the absorption/emission characteristics per the change of design parameters, we obtain optimal design values that secure selectively high emissivity. Localized surface plasmon resonance occurs at the edge of the tungsten circular hole pattern in the high absorption/emission band. This resonance has the effect of confining the magnetic field to the dielectric layer by forming induced loop-like currents. The spectral efficiency of the proposed emitter is 41.0% at 1,000K and 63.7% at 1,300K. It showed five- and three-times higher efficiency, respectively, than the black-body of the same temperature.
윤보라 ( Yoon Bora ) 한국중국언어학회 2020 중국언어연구 Vol.0 No.88
An adjective of Standard Chinese can expand its meaning by duplication. A duplicated adjective tends to strengthen its meaning. During the course of duplication, changes occur not only in its form but in its sound. The primary stress is given to the second syllable, which often changes to the first tone with retroflexion. A monosyllabic adjective can constitute a prosodic foot by duplication. The second syllable changes to the high level tone in order to make it more salient. The retroflexion seems to be caused by the influence from spoken Pekingese. The stress and change of tone in SC duplicated adjectives mean that SC is in the verge of changes. This study is significant because it sheds light to the in the duplicated adjectives while past studies often pay attention only to syntactic, typological, and morphological aspects of them.
KIDP ‘산업디자인통계조사’의 이해 : ’07, ’09 조사의 디자인산업 부분 비교를 중심으로
윤보라(Yoon Bo Ra),이주명(Rhi Joo-myung) 한국디자인학회 2010 한국디자인학회 학술발표대회 논문집 Vol.2010 No.10
In order to let our country, industry, and education fields realize the importance and necessity of design as well as promote the design to them, we need a tool to understand the current status of design industry through scientific and systematic methods, which is also be recognized by Industrial Design Prompotion Act. Since the 1997 survey, a total of five times has been done by the Ministry of Knowledge Economy and Korea Institute of Design Promotion (KIDP)through utilizing various basic materials of related policies. The study is comparing the survey in 2007 with that in 2009 through focus on the design industry, which examines the overall study subjects, duration, scope ,target and methods by analysing survey overview, examines the question of the overall configuration item, the number of items, detailed configuration by analysing survey questions, and examines if there is any change in the size of the design industry, the number of design-related companies, the internationalization status of design field, the distribution of design companies, customer awareness of the design, design-related resources and system by analysing the survey results.
어머니의 미디어 리터러시가 유아의 이야기 이해력에 미치는 영향: 어머니의 스마트미디어 중재의 매개효과
윤보람 ( Boram Yoon ),이영신 ( Youngshin Lee ) 한국유아교육학회 2020 유아교육연구 Vol.40 No.6
본 연구에서는 어머니의 미디어 리터러시가 유아의 이야기 이해력에 미치는 영향에서 어머니의 스마트미디어 중재의 매개효과를 알아보고자 하였다. 이를 위해 서울, 경기, 인천, 대구에 위치한 유치원, 어린이집에 재원 중인 만 5세 유아 123명과 그들의 어머니 123명을 대상으로 설문조사 및 검사를 실시하였다. 수집된 자료는 각 측정 변인들의 기술적 통계치를 산출하고, Pearson의 적률상관분석을 실시하여 각 변인 간의 상관관계를 알아보았으며 매개효과검증을 위해 회귀분석 및 Sobel test를 통한 매개변인의 통계적 유의성을 검증하였다. 연구 결과 첫째, 어머니의 미디어 리터러시와 중재, 유아의 이야기 이해력은 정적 상관으로 나타났다. 둘째, 어머니의 비판적 리터러시가 유아의 이야기 이해력에 미치는 영향에서 어머니의 스마트미디어 중재유형 중 교육적 중재, 제한적 중재, 적극적 중재는 완전매개효과를 가졌다. 결론적으로 어머니가 미디어를 비판적으로 분석하고 이해하는 역량인 비판적 리터러시는 어머니의 교육적. 제한적, 적극적 미디어 중재를 통해 유아의 이야기 이해력에 정적으로 영향을 미쳤으며, 이상의 결과는 스마트미디어 시대에 유아를 위한 건강한 스마트미디어 이용 방침 마련 및 부모 미디어 교육 프로그램 개발을 위한 기초자료를 제공한다. The purpose of this study is to examine the mediated effects of mothers' smart media mediation upon the influence of their media literacy on their children's story comprehension. The subjects included 123 five-year-olds children and their 123 mothers from kindergartens and child-care centers in Seoul, Gyeonggi, Incheon and Daegu. Collected data were analyzed by means of descriptive statistics, pearson's correlation. The mediating effect test proposed by Baron and Kenny(1986) was performed to test if mothers' smart media mediation would play a role as a mediating variable between their media literacy and children's story comprehension. Nest, the sobel test was employed to examine mediating variables for their statistical significance. The results of this study were as follows. First, there were positive correlations between media literacy, smart media mediation, and children's story comprehension. Second, the mediated effects of mothers' smart media mediation showed that their instructive, restrictive, and active mediation had full mediating effects upon the influence of critical literacy on their children's story comprehension. In conclusion, mothers' critical literacy their capabilities of analyzing and understanding the media critically had positive effects on young children's story comprehension through their instructive, restrictive, and active media mediation. These findings imply a need to develop a set of policies for young children's healthy smart media use and a group of media education programs for parents in the era of smart media, providing basic data for such policies and programs.
尹寶羅(Yoon, Bo-ra),金大一(Kim, Dae-il),嚴翼相(Eom, Ik-sang) 중국어문학연구회 2021 중국어문학논집 Vol.- No.127
This article demonstrates the prosodic differences between reduplicated verbs and adjectives in Standard Chinese (hereafter SC). The second syllable of a reduplicated verb, e.g. zouzou ‘take a stroll’, or a reduplicated adjective, e.g. gaogao-xingxing ‘delighted’, is described in grammars of SC as often pronounced in the neutral tone. A phonetic experiment on four speakers of Beijing Mandarin using Praat indicates a distinction between the second syllable of reduplicated verbs and reduplicated adjectives. The second syllable of the reduplicated verb has a pitch height determined by the tone of the preceding syllable. In contrast, the second syllable of the reduplicated adjective is pronounced in a relatively high tone regardless of the tonal category of the preceding syllable. Accordingly, this article claims that the former is indeed the neutral tone, which has features [+tone, -stress], while the latter is an unstressed syllable, which has features [-tone, -stress]. This prosodic difference is closely related to the semantic difference. A reduplicated verb indicates a casual or brief action (zou ‘to walk’; zouzou ‘take a stroll’) while a reduplicated adjective indicates intensity or vividness (gaoxing ‘happy’, gaogao-xingxing ‘delighted’). This demonstrates the interface in SC of phonetics and phonology on the one hand with morphology, pragmatics, and semantics on the other.