싸리나무 추출물의 Xanthoangelol 분리 및 HPLC-DAD 밸리데이션

우현심,김영수,오유진,조해진,송세규,김대욱 한국식품과학회 2020 한국식품과학회지 Vol.52 No.1

This study was undertaken to determine the characteristics of xanthoangelol, the major chalcone constituent derived from the extracts of different parts of Lespedeza bicolor. Xanthoangelol was isolated from the root extract using column chromatography and used as a standard for quantitative analysis. The structure of the isolated compound was established based on spectroscopic evidence. The HPLC-DAD method was validated for specificity, linearity, precision, accuracy, limit of detection, and limit of quantitation. The calibration curve of xanthoangelol had significant linearity (R2>0.9999). Limit of detection and limit of quantitation 0.018 and 0.059 μg/mL, respectively. The relative standard deviation values of precision test, and intra- and inter-day tests were less than 0.22 and 0.40%, respectively. In the recovery test, the accuracy ranged from 98.98-102.78% with RSD values less than 0.13%. The method validation parameters indicate the applicability of the HPLC method for quality control of food or drug formulations containing L. bicolor.

LPS로 유도된 RAW264.7 대식세포에 대한헛개나무(Hovenia dulcis) 추출물의 항염증 효과

우현심,이선민,허정두,이민성,김영수,김대욱 한국자원식물학회 2018 한국자원식물학회지 Vol.31 No.5

In this study, the anti-inflammatory activities of the extracts of different parts of Hovenia dulcis such as leaves, stems, and roots were investigated. Among them, the roots extract (RE) showed the most potent suppressive effect against pro-inflammatory mediators in LPS-stimulated mouse macrophage cells. RE induced dose-dependent reduction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and concomitantly reduced the production of NO and PGE2. Additionally, pre-treatment with RE significantly suppressed the production of inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6, as well as mRNA levels. Moreover, phosphorylation of mitogen-activated protein kinases (MAPKs) and nuclear translocation of nuclear factor-kappa B (NF-kB) were also strongly attenuated by RE in RAW264.7 cell. Furthermore, RE induced HO-1 expression through nuclear translocation of nuclear factor E2-related factor 2 (Nrf2) and increase HO-1 activity in RAW264.7 macrophages. Therefore, these results indicate that RE strongly inhibits LPS-induced inflammatory responses by blocking NF-kB activation, inhibiting MAPKs phosphorylation, and enhancing HO-1 expression in macrophages, suggesting that RE of H. dulicis and a major component, 27-O-protocatechuoylbetulinic acid could be applied as a valuable natural anti-inflammatory material. 본 연구는 헛개나무 잎, 줄기, 뿌리로부터 얻어진 메탄올 추출물의 항염증 효과를 구명하기 위해서 수행되었다. LPS로 염증이 유도된 RAW264.7 세포 내 부위별 추출물을 동시에 처리하여 염증 매개성 물질인 NO 생성량 분석 결과 40 ㎍/㎖ 농도에서뿌리(94.7%)와 줄기(42.6%)에서는 효과가 있는 반면 잎에서는효과가 없는 것으로 확인되었다. 이 중 탁월한 효과를 보인 뿌리추출물(RE)의 항염증 효과 및 관련 분자적 기전을 확인하였다. 그 결과, 염증 반응의 주요 경로인 NF-kB 및 MAPK 신호전달경로에서 RE가 LPS로 유도된 NF-kB의 핵 이동을 억제하고, ERK, JNK, p38의 인산화를 억제함으로써 iNOS, COX-2의 발현이 감소되고, NO와 pro-inflammatory cytokine (IL-6, IL-1β, TNF-α)의 생성이 억제됨을 확인하였다. 또한 RE는 대식세포에서 Nrf2를 활성화시켜 항염증성 단백질인 HO-1 발현을 유도하여 항염증 효과를 나타내었다. 또한, RE로부터 주요 성분을 분리한 후 NMR과 MS 기기를 이용하여 구조 동정된 27-Oprotocatechuoylbetulinic acid 화합물에서도 높은 항염증 효과를 확인하였다. 이러한 연구결과는 헛개나무뿌리와 그 주요성분은 의약품 소재 및 기능성 식품 등의 기능성 소재로 활용될수 있는 기초적인 정보를 제공할 것으로 생각된다.

갈대이삭으로부터 Tricin의 분리 및 함량 분석

우현심,이승영,황병수,정상철,김대욱 한국생약학회 2017 생약학회지 Vol.48 No.1

The aim of this study was to investigate a validation method for determination of tricin in Phragmites communis ears. Tricin was isolated with chromatographic methods and used as the standard substances for quantitative analysis. The structural determination was characterized by comparing their NMR spectral data with values reported in the literature. For validation, the specificity, linearity, precision, accuracy, detection limits, and quantification limits of tricin was measured by HPLC. The results show that the specificity was satisfied with retention time and diode array detector (DAD) spectrum by analysis of tricin using HPLC. The limits of detection (LOD) for tricin was 0.84 mg/ml. Recovery of tricin was 98.80~108.22% with R.S.D values less than 2%. Intra-day and inter-day precisions of tricin in P. communis ears were 99.96~100.96% and 99.01~100.40%, respectively. Therefore, application of tricin was validated by an analytical method as major compound in P. communis ears.

방사선 유도 돌연변이체, 블랙베리 발효음료의알코올 대사 및 숙취 억제 효과

조병옥,소양강,이창욱,조정근,우현심,진창현,정일윤 한국방사선산업학회 2013 방사선산업학회지 Vol.7 No.1

This study was designed to elucidate the effect of fermented blackberry drinks (BD) onalcohol metabolism and hangover in alcohol-treated rats. We showed that the administration ofBD increased the activity of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) inalcohol-treated rats. Moreover, the administration of BD reduced the serum alcohol and acetaldehydeconcentrations in alcohol-treated rats. Furthermore, the administration of BD attenuatedthe levels of serum aspartate aminotransferase and alanine aminotransferase in alcohol-treatedrats. Taken together, these results suggest that BD plays an important role in alcohol metabolismand liver function by reducing blood alcohol and acetaldehyde through the activation of ADH andALDH in alcohol-treated rats and could be used as a functional anti-hangover drinks.

방사선 유도 돌연변이체 블랙베리로 제조한발효음료의 간 손상 회복 효과

조병옥,소양강,이창욱,조정근,우현심,진창현,정일윤 한국방사선산업학회 2013 방사선산업학회지 Vol.7 No.1

This study was conducted to investigate the effect of fermented blackberry drinks (BD)on carbon tetrachloride (CCl4)-induced liver injury in rats. Male Sprague-Dawley rats were randomlydivided into four groups with 6 rats per group: control, CCl4, CCl4 plus BD 3 ml kg-1, andCCl4 plus BD 6 ml kg-1. We found that the levels of serum aspartate aminotransferase (AST) andalanine aminotransferase (ALT) were significantly increased and the activity of antioxidant enzymeglutathione peroxidase (GPx) in the liver was decreased in rats treated with CCl4 alone when comparedwith the control group. However, the administration of BD attenuated the levels of serumAST and ALT in CCl4-treated rats. Moreover, the administration of BD significantly increasedthe activity of GPx in CCl4-treated rat livers. Taken together, these results suggest that BD couldprotect the liver from CCl4-induced hepatic damage.

HPLC에 의한 차나무 잔가지(부산물)의 추출물 내 지표 성분의 정량분석

이민성,임현정,정희석,조해진,우현심,오유진,이수인,김현철,안경환,김영수,김대욱 한국약용작물학회 2019 한국약용작물학회지 Vol.27 No.1

Background: Camellia sinensis L.(CS) is a perennial evergreen species of plant whose leaves are used to produce tea. In this plant species, the parts used are the leaves, sub-branch parts are thrown out. Methods and Results: Ethanol extract of sub-branch parts was used for isolation of major compounds by column chromatography. Structures were identified as caffeine (1), (-)-epicatechin (2) and (-)-epicatechin gallate (3) by interpretation of spectroscopic analysis, including 1H- and 13C-NMR. High-performance liquid chromatography (HPLC) method was used to compare the quantitative level of marker compounds in various extraction solvents of sub-branch parts of CS. The content of caffeine, (-)-epicatechin, and (-)epicatechin gallate in 30% ethanol extract showed higher value with 3.28 ± 0.57 ㎎/g, 5.53 ± 0.88 ㎎/g, and 1.29 ± 0.24 ㎎/g, respectively. Conclusions: These results indicated that not only leaves parts but also sub-branch, could be a good source for the functional material and pharmaceutical industry.

지렁쿠나무 메탄올 추출물의 생리활성 연구

오유진(Yu Jin Oh),조해진(Hae Jin Cho),우현심(Hyun Sim Woo),변준기(Jun-Gi Byeon),김영수(Yeong-Su Kim),김대욱(Dae Wook Kim) 한국생명과학회 2020 생명과학회지 Vol.30 No.11

한국, 중국, 일본에 분포한 지렁쿠나무는 관절염 치료제로 동양의 민간요법에 사용되었다. 본 연구에서는 지렁쿠나무의 건강기능성 식품, 화장품 및 식품 보존료 등의 기능성 소재로 개발하기 위한 기초자료를 제공하고자 연구를 수행하였다. 지렁쿠나무의 잎, 가지 부위를 메탄올 추출물로 제조하여 총 폴리페놀 및 총 플라보노이드 함량을 측정하고, 항산화 활성, α-glucosidase 저해 활성 및 tyrosinase 저해 활성과 항염증 활성을 검증하였다. 지렁쿠나무 추출물의 총 폴리페놀 및 플라보노이드 함량은 1.52±0.1 mg/g and 1.73±0.1 mg/g로 측정되었고, DPPH와 ABTS radical 소거 활성은 처리 농도가 증가함에 따라 농도 의존적으로 소거 효과를 나타냈으며, 각각 IC50 값이 124.0 μg/ml (DPPH), 85.6 μg/ml (ABTS)로 높은 항산화 효과를 나타내었다. 또한, α-glucosidase 저해활성 및 tyrosinase 저해 활성은 각각 IC50 값이 183.5 μg/ml와 323.9 μg/ml로 우수한 억제 활성을 보였다. 게다가 RAW 264.7 cell에서 LPS로 염증을 유발시켜 nitric oxide (NO) 억제효과 실험에서 지렁쿠나무 추출물의 IC50 값을 측정하였을 때 NO 생성을 농도의존적으로 저해하는 농도는 36.7 μg/ml이고, 추출물 100 μg/ml의 농도에서도 독성을 나타내지 않는 것으로 확인되었다. 이상의 결과로부터 지렁쿠나무 추출물은 항산화, 항염증, 미백, 및 항당뇨 효능을 확인할 수 있었으며, 이는 지렁쿠나무가 기능성 식품, 화장품 및 의약품 소재로서의 활용 가능성을 시사한다. 또한, 지렁쿠나무로부터 생리활성물질의 분리 동정과 메커니즘 규명에 관한 연구가 수행되어야 할 것으로 판단된다. Sambucus sieboldiana var. miquelii (Nakai) Hara is distributed in Korea, China, and Japan, and has been used as an anti-rheumatic in folk medicine in oriental countries. The present study aims to investigate the potential use of this species in health functional foods, cosmetics, and food preservatives. Methanol extracts of leaves and branches from this plant were prepared to quantitatively analyze the total phenol and flavonoid contents, and to investigate the antioxidative and enzyme inhibitory activities, and the inhibition of nitric oxide (NO) production activity. The results showed that the total polyphenol and flavonoid contents of the crude extract were 1.52±0.1 mg/g and 1.73±0.1 mg/g, respectively. S. sieboldiana polyphenols exhibited potent scavenging activity shown by 2, 2’-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and 2, 2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation assay. The crude extract also exhibited significant α-glucosidase and tyrosinase inhibitory activity with IC50 values of 183.5 μg/ml and 323.9 μg/ ml, respectively. Additionally, the crude extract exhibited strong anti-inflammatory activity determined through the nitric oxide inhibition assay in a dose-dependent manner with an IC50 value of 36.7 μg/ml and no cytotoxic effect on the macrophages. Therefore, we demonstrated that the leaves and branches of S. sieboldiana extract possess antioxidant, anti-diabetic, depigmentation potential, and NO production inhibitory activities. According to recent research, S. sieboldiana has great potential as a source of the bioactive compound which could be used as food, cosmetics, and pharmaceutical agents.