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우은란,신지은,김홍광,김청룡 한국생약학회 2011 생약학회지 Vol.42 No.3
Chaenomeles sinensis Koehne (Rosaceae) is a deciduous tree and is distributed in China, Korea and Japan. In previous studies on the fruits of C. sinensis, some triterpenoid compouds such as oleanolic acid, tormentic acid were reported. In an ongoing investigation into biologically active compounds from natural products, the methanol extract of the stems of C. sinensis was investigated. By means of the repeated column chromatography using silica gel, Sephadex LH-20, LiChroprep RP-18, betulin (1), tormentic acid (2), 1-β-D-glucopyranosyl-3,4,5-trimethoxybenzene (3), lyoniresinol-2a-O-α-L-rhamnopyranoside (4) were isolated. The chemical structures of compounds 1-4 were determined by the basis of physico-chemical properties and spectroscopic methods such as 1D and 2D NMR. For the isolated compounds (1-4), the inhibitory activity of IL-6production in TNF-α stimulated MG-63 cell was examined. Among the isolates, betulin (1), 1-β-D-glucopyranosyl-3,4,5-trimethoxybenzene (3), lyoniresinol-2a-O-α-L-rhamnopyranoside (4) showed inhibitory effects on IL-6 production in TNF-αstimulated MG-63 cell.
수종의 생약이 과화산화수소에 의한 Hypoxanthine-guanine Phosphoribosyl Transferase (HPRT) 유전자 돌연변이 억제에 미치는 효과
유호진,우은란 한국생약학회 2004 생약학회지 Vol.35 No.1
DNA damage induced by reactive oxygen species (ROS) seems to play an important role in the induction of muta-tion and cancer. Hydrogen peroxide (H2O2) has been shown to induce a variety of genetic alterations, probably by the generationof hydroxyl radicals via Fenton reaction. In this study, we examined the ability of medicinal herbs in the suppression of H2O2-induced mutagenesis. Human fibroblast GM00637 cells were treated with H2O2 in the presence or absence of medicinal herbs,and H2O2-induced mutant frequency was measured at the hypoxanthine guanine phosphoribosyltransferase (HPRT) locus.Treatment of cells with various doses of H2O2 caused a significant increase of the HPRT mutant frequency. However, pre-treatment of cells with several medicinal herbs reduced H2O2-induced mutant frequency. The strong antimutagenic effects wereobserved from the methylene chloride and ethyl acetate fractions of Selaginella tamariscina, Panax ginseng, and Angelica acuti-loba; ethyl acetate fractions of Rehmania glutinosa, Leonurus sibiricus, Curcuma zedoaria and Commiphora molmol; butanolfractions of Scutellaria barbata, Tribulus terrestris, Curcuma zedoaria, Cyperus rotundus and Carthamus tinctorius, whichwere more than 60% inhibition of H2O2-induced mutant frequency at the HPRT locus.
문영희,우은란,박영준 한국생약학회 2005 생약학회지 Vol.36 No.4
Sarcodon aspratus (Thelepholaceae), a native mushroom, is distributed in Korea and Japan, and has been widely used in traditional food and folk medicines. To confirm the biological activities of Sarcodon aspratus, the liver protecting activity, anti-clotting activity, and anti-complementary activity of the water extract, EtOH extract, and the water soluble proteoglycan part of S. aspratus were investigated. The EtOH, and water extract of S. aspratus decreased the GOT and GPT releases induced by CCl4 in a dose-dependent manner. On the other hand, the water soluble proteoglycan part of S. aspratus showed weak inhibitory activity. In addition, the EtOH and water extract of S. aspratus prevented CCl4-induced hepatotoxicity, as described by a liver histopathologic study. To confirm the anti-clotting activity, the APTT and PT assay were carried out. As a result, only the crude proteoglycan part of S. aspratus showed the anti-coagulating activity, and this result might be due to the inhibition of intrinsic clotting system. Also, the crude proteoglycan part of S. aspratus showed the anti-complementary activity, and the IC50 value was 50 µl/ml.
고재종,우은란,문영희 한국생약학회 2004 생약학회지 Vol.35 No.3
Repeated column chromatography of an ethyl acetate extract of the root bark of Albizzia julibrissin Durazz afforded four compounds, euscaphic acid ester glucoside (1), luteolin-7-O-neohesperidoside (2), (+)-medioresinol (3), (-)-syringaresinol (4). Their structures were determined by chemical and spectroscopic methods. Compounds 1-4 were isolated from this plant for the first time. Among these compounds, (+)-medioresinol (3) exhibited moderate cytotoxic activity against XF 498 and HCT 15 cell line.
임성우,안덕균,우은란,박호균,Lim, Seong-Woo,Ahn, Duk-Kyun,Woo, Eun-Rhan,Park, Ho-Koon The Society of Korean Medicine 1998 대한한의학회지 Vol.19 No.2
면역 결핍 바이러스와 허피즈 바이러스 -1,2에 대한 한약의 항바이러스 작용을 관찰하기 위하여, 세포 바이러스 검색에 초기 독성과 감염 바이러스의 생존 세포 수를 비교적 단기간내 볼 수 있는 96-well plate를 이용한 MTT assay로 측정하였다. 본 실험은 예비 실험 단계에서 사용된 총 85가지의 한약중 단미제 6가지와 복합처방 44가지를 선정하였으며 복합처방은 유사한 치법으로 분류하였고 한약의 시료 추출은 순수하게 물로 전탕하여 여과하였다. 실험 결과 파두와 맥아가 면액 결핍 바이러스에 대해 감염초기 유의성이 있었으며 호장근, 갈근우방자탕과 형방패독산이 허피즈 바이러스-1,2에 대해 감염초기 유의성이 있었으나 실험의 시간 경과에 따른 지속적인 약효 안정성을 보이지는 못하였다. 이 실험을 통하여 한약을 이용한 우수한 바이러스 치료를 개발하기 위해서는 단미제나 복합 처방에서 항바이러스 작용이 큰 유효성분의 대량 분리와 세포 실험에 있어서 오차를 줄 일 수 있는 세포면역학적 실험의 도입 등이 필요할 것으로 사료된다.
Chemical Constituents from the Aerial Parts of Aster yomena
Qinglong Jin,우은란,Hae Ju Ko,Young-Su Chang 한국생약학회 2013 Natural Product Sciences Vol.19 No.3
Nine terpenoids, spinasterone (1), simiarenol (2), phytol (3), lupeol (4), a-amyrin (5), 1b,4b-dihydroxyeudesman-11-ene (6), 3,7-dihydroxyhumula-4,8(15),10(E)-triene (7), 2,6-dihydroxyhumula-3(12),7(13), 9E-triene (8), 23-hydroxybetulin (9) were isolated from the aerial parts of Aster yomena M. Their structures were identified based on 1D and 2D NMR, including 1H-1H COSY, HSQC, HMBC and NOESY spectroscopic analyses. Compounds 1 - 9 were isolated from this plant for the first time.
Cana Park,우은란,이동건 한국분자세포생물학회 2010 Molecules and cells Vol.29 No.6
Styraxjaponoside C was investigated with respect to its antifungal activity and mechanisms of action. Devoid of hemolytic activity, Styraxjaponoside C demonstrated an antifungal effect against the human pathogenic yeast Can-dida albicans in an energy-independent manner. To char-acterize the mechanisms of the antifungal activity of Styraxjaponoside C, fluorescence analysis with membrane probe 1,6-diphenyl-1,3,5-hexatriene, and flow cytometric analysis on C. albicans were conducted. The results showed that Styraxjaponosdie C induced cytoplasmic membrane perturbation. The current study suggested that Styraxjaponoside C was active against C. albicans with membrane-active mechanisms.