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예완해,박홍현,심홍식,남영주,김설아,이정희,김용기,이용환,이영훈 한국식물병리학회 2008 식물병연구 Vol.14 No.1
This study was conducted to decide disease incidence level of rice leaf blast required for reasonable fungicide application in paddy field. We induced the disease development by inoculating rice blast pathogens on rice seedlings (Jinmibyeo) in the greenhouse and transplanting the infected seedlings in the field two weeks after rice plants were transplanted. We scored the disease incidence, grouped and marked according to degree of percentage of diseased leaf area at maximum stage of disease development. The percent diseased leaf area (PDLA) had negative correlations with panicle number per hill, ripened grain (%), and total yield; their correlation coefficients (r), −0.97**, −1.00** and −0.96**, respectively. However, it had positive correlations with spikelets per panicle and thousand grain weight; their correlation coefficients (r), 0.98** and 0.98**, respectively. Gain threshold (GT) calculated based on control cost and market price was estimated to be 8.35. Economic injury level (EIL) obtained based on GT and coefficient of damage of regression equation between disease incidence and the different yield components; panicle number per hill, spikelets per panical, ripened grain(%), thousand grain weight and yield were 41.8, 9.7, 19.1, 291.1 and 3.4%, respectively. Economic threshold (ET) for yield was 2.7% (3.4%(EIL) × 0.8) on PDLA. These results suggest that application of fungicide is necessary when two under leaves are almost covered with lesions or contained more than twenty large lesions under leaves at maximum tillering stage. This study was conducted to decide disease incidence level of rice leaf blast required for reasonable fungicide application in paddy field. We induced the disease development by inoculating rice blast pathogens on rice seedlings (Jinmibyeo) in the greenhouse and transplanting the infected seedlings in the field two weeks after rice plants were transplanted. We scored the disease incidence, grouped and marked according to degree of percentage of diseased leaf area at maximum stage of disease development. The percent diseased leaf area (PDLA) had negative correlations with panicle number per hill, ripened grain (%), and total yield; their correlation coefficients (r), −0.97**, −1.00** and −0.96**, respectively. However, it had positive correlations with spikelets per panicle and thousand grain weight; their correlation coefficients (r), 0.98** and 0.98**, respectively. Gain threshold (GT) calculated based on control cost and market price was estimated to be 8.35. Economic injury level (EIL) obtained based on GT and coefficient of damage of regression equation between disease incidence and the different yield components; panicle number per hill, spikelets per panical, ripened grain(%), thousand grain weight and yield were 41.8, 9.7, 19.1, 291.1 and 3.4%, respectively. Economic threshold (ET) for yield was 2.7% (3.4%(EIL) × 0.8) on PDLA. These results suggest that application of fungicide is necessary when two under leaves are almost covered with lesions or contained more than twenty large lesions under leaves at maximum tillering stage.
예완해,지형진,심홍식,남기웅,이훈섭,김용기 한국식물병리학회 2004 식물병연구 Vol.10 No.2
This study was carried out to investigate factors affecting disease development of rice brown spot caused by Cochliobolus miyabeanus in the field and under the greenhouse conditions. In normal paddy fields, the pathogen was more frequently isolated from dried rice straws than wet straws after overwintering. The pathogen was also readily isolated from wet rice straws of salty paddy fields although infection rate of the fields was low. The disease symptom was first observed at 2 to 3days after inoculation when the seedlings were kept for 6 hours in dew chamber. The number of lesions peaked at 14 hr incubation in cultivars Chucheongbyeo and 24 hr incubation in cultivar Daesanbyeo and Tetep. Sixty three-d-old seedlings were more susceptible than 21 to 49-d-old seedlings regardless of cultivar. There was some difference in number of lesions observed, but the lesions were evenly spread regardless of leaf position. Light on the rice seedlings at 3,000 lux or 6,000 lux showed more severe symptoms than those in the dark.
벼 깨씨무늬병 발병정도에 따른 논토양, 벼알 및 볏짚에서의 무기성분 비교
예완해,박양호,김이열,탁정순,남영주,심홍식,김용기,연병렬 한국식물병리학회 2009 식물병연구 Vol.15 No.1
In order to study a relationship between soil nutrients and rice brown spot occurrence, paddy field soils, rice grains and straws collected from different paddy fields with different disease degrees of brown spots were analyzed for inorganic nutrients. Brown spot was prevalent in the rice grown in nutrient-deficient soils, which is especially low in macronutrient elements (phosphoric acid, potassium, silicic acids) and micronurients (calcium, magnesium). The soil, however, was high in sodium while organic nutrients and pH level were similar to others. The rice straws with severe brown spot were low in inorganics such as ferrous, copper, T-N, and P2O5 while the rice grains with brown spot were low in ferrous, MgO, Zn, and Mn. In the analysis of field type and nitrogen level, the highest disease severity was found in sandy-type field soil, followed by salty-type field soil and disease severity decreased as application level of nitrogen fertilizer increased. As a summary, the most important factor for effective brown spot control in rice is maintenance of proper nutrients in sandy-type field and control of sodium level in salty-type field soil. In order to study a relationship between soil nutrients and rice brown spot occurrence, paddy field soils, rice grains and straws collected from different paddy fields with different disease degrees of brown spots were analyzed for inorganic nutrients. Brown spot was prevalent in the rice grown in nutrient-deficient soils, which is especially low in macronutrient elements (phosphoric acid, potassium, silicic acids) and micronurients (calcium, magnesium). The soil, however, was high in sodium while organic nutrients and pH level were similar to others. The rice straws with severe brown spot were low in inorganics such as ferrous, copper, T-N, and P2O5 while the rice grains with brown spot were low in ferrous, MgO, Zn, and Mn. In the analysis of field type and nitrogen level, the highest disease severity was found in sandy-type field soil, followed by salty-type field soil and disease severity decreased as application level of nitrogen fertilizer increased. As a summary, the most important factor for effective brown spot control in rice is maintenance of proper nutrients in sandy-type field and control of sodium level in salty-type field soil.
pBRG-4를 이용한 Metarhizium anisopliae의 형질전환
이동규,예완해,황철원,권석태,강선철,Lee, Dong-Gyu,Yeh, Wan-Hae,Hwang, Cher-Won,Kwon, Suk-Tae,Kang, Sun-Chul 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.33 No.2
작물 병해충에 대한 무공해 미생물 농약 개발의 일환으로 곤충 병원성 곰팡이 Metarhizium anisopliae의 분자생물학적 육종을 위해 이 균주의 형질전환계를 확립하였다. M. anisopliae의 원형질체를 제작하여 benomyl 약제에 대하여 저항성을 나타내는 Aspergillus flavus 유래의 ${\beta}-tubulin$ 유전자를 갖는 pBRG-4 plasmid DNA를 polyethylene glycol 방법으로 형질 전환하였다. 이 형질전환은 pBRG-4 DNA $50\;{\mu}g$당 10개의 효율로 이루어졌으며, 그 결과 야생형 균주들의 $2.5\;{\mu}g/ml$ 농도의 benomyl 존재 하에서도 성장이 억제되는데 반하여 선발된 형질전환체들은 $5.0\;{\mu}g/ml$ 농도의 benomyl 함유 배지에서도 잘 성장하였다. 또한 이 형질전환체들의 chromosomal DNA를 분리하여 Southern 분석한 결과 ${\beta}-tubulin$ 유전자가 homologous recombination에 의하여 M. anisopliae의 genome속에 삽입 되었음을 확인하였다. We have established a transformation system for entomopathogenic fungus, Metarhizium anisopliae, in order to develop mycoinsecticide by recombinant DNA techniques. Protoplasts of M. anisopliae would be transformed to a benomyl-resistant by introducing pBRG-4 plasmid DNA, which contains a ${\beta}-tubulin$ gene of Aspergillus flavus conferring resistance to benomyl and a pyr4 gene of Neurospora crassa, in the presence of 5% polyethylene glycol and 10 mM calcium chloride. Transformants occuring at a frequency of 10 colonies per $50\;{\mu}g$ pBRG-4 DNA grew on the $5\;{\mu}g/ml$ concentrations of benamyl, while the wild type was inhibited by $2.5\;{\mu}g/ml$. From the Southern analysis using genomic DNAs isolated from M. anisopliae transformants, the positive signals suggested that the ${\beta}-tubulin$ gene had integrated in the M. anisopliae genome by homologous recombination.