Molecular Taxonomy of a Phantom Midge Species (Chaoborus flavicans) in Korea

안해인,정길아,김창배 한국동물분류학회 2012 Animal Systematics, Evolution and Diversity Vol.28 No.1

The larvae of Chaoborus are widely distributed in lakes, ponds, and reservoirs. These omnivorous Chaoborus larvae are crucial predators and play a role in structuring zooplankton communities, especially for small-sized prey. Larvae of Chaoborus are commonly known to produce predator-induced polyphenism in Daphnia sp. Nevertheless, their taxonomy and molecular phylogeny are very poorly understood. As a fundamental study for understanding the role of Chaoborus in predator-prey interactions in a freshwater ecosystem, the molecular identification and phylogenetic relationship of Chaoborus were analyzed in this study. A molecular comparison based on partial mitochondrial cytochrome oxidase I (COI) between species in Chaoborus was carried out for the identification of Chaoborus larvae collected from 2 localities in Korea. According to the results, the Chaoborus species examined here was identified as C. flavicans, which is a lake-dwelling species. Furthermore,partial mitochondrial genome including COI, COII, ATP6, ATP8, COIII, and ND3 were also newly sequenced from the species and concatenated 5 gene sequences excluding ATP8 with another 9 dipteran species were compared to examine phylogenetic relationships of C. flavicans. The results suggested that Chaoborus was more related to the Ceratopogonidae than to the Culicidae. Further analysis based on complete mitochondrial DNA sequences and nuclear gene sequences will provide a more robust validation of the phylogenetic relationships of Chaoborus within dipteran lineages.

한의 표준임상진료지침 및 한의 표준임상경로에 대한 한의사의 인식

김동수,안해인,권수현,안은지,김남권 대한예방한의학회 2022 대한예방한의학회지 Vol.26 No.2

Objectives : This study is aimed to survey Korean Medicine Doctors’s perceptions in Korean Medicine Clinical Practice Guideline and Standard Korean Medicine Clinical Pathways for its spread(to increase utilization in clinical sites). Methods : The research population was set at 14,831 Korean medical institutions registered with the Health Insurance Review and Assessment Service for sampling representative of Korean Medicine Doctors, and the final 2,007 subjects were selected in consideration of the research period and budget. This survey was conducted based on a telephone survey, and in some cases, a fax or e-mail survey was also conducted together. Six questions were asked about the perception of ‘Korean Medicine Clinical Practice Guideline and Standard Korean Medicine Clinical Pathways’, and three questions about the characteristics of the respondent. Results : The rate of recognizing Korean Medicine Clinical Practice Guideline was 36.1%. Those who worked at Korean medicine hospitals, were under 39 years of age, and had less than 11-20 years of experience as an Korean medical doctor were more likely to be aware of it. Regarding the experience of using Korean Medicine Clinical Practice Guideline, the rate of ‘not used in the past and not used now’ was very high at 82.9%, but the intention to use it in the future was high at 60.7%. About the Korean Medicine Clinical Pathways, 79.9% of respondents answered that they did not know. 80.6% of respondents recognized the need for the development of clinical manuals such as Korean Medicine Clinical Practice Guideline and Standard Korean Medicine Clinical Pathways. Conclusion : Their low awareness of Korean Medicine Clinical Practice Guideline seems to affect its low utilization. Therefore, active education and public relations are required in the future.

Goligher 분류상 1기-3기에 해당하는 항문질환 환자의 한의치료 증례군보고

김찬영,성강욱,안해인,윤영흠,김남권 대한한방내과학회 2020 大韓韓方內科學會誌 Vol.41 No.6

Background: Hemorrhoid surgery is a frequently performed surgery in Korea, second only to cataract surgery. However, it has serious complications, including bleeding, infection, anal stenosis, and urinary retention, so active conservative therapies are in need at an early stage. Case summary: Thirteen patients with anal disease complained of main symptoms of pain, bleeding, and prolapse, with additional complaints of itching and mucus discharge. An herbal medicine mixture of Eulja-tang and Hwangryeonhaedok-tang was administered twice daily, and dry cupping therapy, acupuncture, and hot pack placements were administered on pelvic and sacral regions once weekly for four weeks. The symptoms were evaluated before and each week after the treatments. The treatments had statistically significant therapeutic effects. Conclusion: Korean medicine treatment—effectively alleviating the symptoms of anal disease, such as pain, bleeding, and prolapse—may represent an alternative therapy for conservative treatments in the early stages of anal disease.

Differential Tissue-specific and Pathway-specific Anti-obesity Effects of Green Tea and Taeumjowitang, a Traditional Korean Medicine, in Mice

김준일,박수진,안해인,최지영,최명숙,최상운,김성진 대한암예방학회 2017 Journal of cancer prevention Vol.22 No.3

Background: Traditional medicines have been leveraged for the treatment and prevention of obesity, one of the fastest growing diseases in the world. However, the exact mechanisms underlying the effects of traditional medicine on obesity are not yet fully understood. Methods: We produced the transcriptomes of epididymal white adipose tissue (eWAT), liver, muscle, and hypothalamus harvested from mice fed a normal diet, high-fat-diet alone, high-fat-diet together with green tea, or a high-fat-diet together with Taeumjowitang, a traditional Korean medicine. Results: We found tissue-specific gene expression patterns as follows: (i) the eWAT transcriptome was more significantly altered by Taeumjowitang than by green tea, (ii) the liver transcriptome was similarly altered by Taeumjowitang and green tea, and (iii) both the muscle and hypothalamus transcriptomes were more significantly altered by green tea than Taeumjowitang. We then applied integrated network analyses, which revealed that functional networks associated with lymphocyte activation were more effectively regulated by Taeumjowitang than by green tea in the eWAT. In contrast, green tea was a more effective regulator of functional networks associated with glucose metabolic processes in the eWAT. Conclusions: Taeumjowitang and green tea have a differential tissue-specific and pathway-specific therapeutic effect on obesity.

추나요법을 포함한 한의학적 치료로 호전된 드퀘르벵 건초염 2예: 초음파 전후 관찰 비교

배준형 ( Jun-hyeong Bae ),이성준 ( Sung Joon Lee ),변상현 ( Sang Hyun Byun ),안해인 ( Hae In Ahn ),김남권 ( Namkwen Kim ) 한방재활의학과학회 2021 한방재활의학과학회지 Vol.31 No.4

This study reports the effectiveness of Korean medicine and Chuna manual therapy on patients with De Quervain’s tenosynovitis. Two patients were treated with Chuna manual therapy, electro-acupuncture, moxibustion therapy. We used numeric rating scale (NRS), Finkelstein’s test, sonography to measure changes during treatment. After each treatment, NRS, Finkelstein’s test, sonography outcome were improved. Korean medicine including Chuna manual therapy can be effectively used to improve De Quervain’s tenosynovitis. This study may suggest that korean medicine including Chuna manual therapy can be effective for De Quervain’s tenosynovitis. (J Korean Med Rehabil 2021;31(4):211-219)

한방 치료로 고안드로겐혈증을 개선한 다낭성 난소 증후군 연속증례연구

신혜규 ( Haegue Shin ),배지용 ( Ji-yong Bae ),지영근 ( Young-geun Ji ),안해인 ( Hae In Ahn ),윤영흠 ( Youngheum Yoon ),김남권 ( Namkwen Kim ) 대한한방부인과학회 2021 大韓韓方婦人科學會誌 Vol.34 No.3

Objectives: This study aims to analyze the medical record of eight cases of polycystic ovary syndrome (PCOS) in a local Korean medicine clinic, in order to provide evidence on Korean medicine treatment of PCOS and to suggest the direction of future studies. Methods: The medical records of eight cases diagnosed with PCOS based on 2003 Rotterdam Criteria and whose total testosterone level was 0.53 ng/ml and over were retrospectively analyzed. The primary outcome was total testosterone level, and the secondary outcomes included luteinizing hormone (LH) and follicle stimulating hormone (FSH) levels, LH/FSH ratio, weight, last mentrual period (LMP), numerical rating scale (NRS) score of dysmenorrhea, and other associated symptoms pre- and post-treatment. Korean medicine treatment consists of the complex herbal prescription consisting of Yukmijihwang-tang, Baekho-tang, Daesiho-tang, and Gyejibokryeong-hwan, modified according to symptoms; acupuncture and electroacupuncture on 中脘(CV12), 下脘(CV10), 關元(CV4), 石門(CV5), 合谷(LI4), 太衝(LR3), 三陰交(SP6), and 懸鍾(GB39); and indirect moxibustion on 神闕(CV8). Results: The average total testosterone level lowered statistically significantly after the treatment. The average FSH and LH levels lowered with insignificance, while the average LH/FSH ratio and weight lowered statistically significantly. During the treatment period of three to six months, every patient had the menstrual cycle shortened except for one case; and two cases with infertility problem succeeded in being pregnant. There was no adverse event. Conclusions: The Korean medicine treatment was found effective in treating PCOS, especially regarding hyperandrogenism, amenorrhea or oligomenorrhea, and infertility.