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Casein micelle 의 조성에 관한 연구 2 . Casein micelle 의 침전에 대한 온도의 영향
신동철,전우민,김영교 ( Dong Chul Shin,Woo Min Jeon,Yong Kyo Kim ) 한국축산학회 1984 한국축산학회지 Vol.26 No.4
This experiment was carried out to study on the protein composition of casein micelles. Casein micelles were isolated by different temperature of ultracentrifugation. And the composition of individual caseins fur the fractions was determined by DEAF - cellulose column chromatography, The results are summarized as follows: 1. When skimmilk was centrifuged at 4℃, 8.5℃, 13.5℃, 18℃ and 30℃ for 60 min at 200,000 x g, the values for the sedimented casein micelles were 57.80%, 79.79%, 80.85%, 90.43% and 86.88% respectively. 2. When skimmilk was centrifuged for 60 min at 100,000 x g, the values for the sedimented casein micelles and serum casein were 60.85%, and 39.15% at 20℃ and 40.24% and 59.76% at 4℃. 3. The relative amount of αs-, β-, k- and γ-caseins in serum casein which was not sedimented by centrifugation for 60 min at 100,000 x g was 44%, 29%, 19% and 8% at 20℃ and 38%, 34%, 16% and 12% at 4℃ respectively.
미생물 고정화 담체를 이용한 충전율 및 질산화·탈질 속도에 관한 연구
신동철,서정미,박철휘,Shin, Dong-chul,Seo, Jung-mi,Park, Chul-hwi 대한환경공학회 2018 대한환경공학회지 Vol.40 No.2
본 연구는 특정 미생물을 고정화시킨 PVA (Polyvinyl Alcohol) gel을 이용한 질산화 탈질 반응속도를 분석하였다. 질산화용 담체는 질산화 미생물인 Nitrosomonas europaea, Nitrobacter spp.를 혼합 배양하여 고정화시켰으며 탈질용 담체는 탈질 미생물인 Psuedomonas, Bacillus, Paracoccus를 고정화시켰다. 미생물 고정화 담체의 효율을 분석하기 위하여 우선 충전율 결정결과 15% (v/v)이 효과적으로 나타났다. 질산화 반응속도는 $12.53mg\;{NH_4}^+-N/L{\cdot}hr$, 탈질 반응속도는 $20.37mg\;{NO_3}^--N/L{\cdot}hr$로 기존 반응속도(질산화 : $3.93mg\;{NH_4}^+-N/L{\cdot}hr$)보다 빠르게 나타났다. 미생물의 고정화 여부를 확인하기 위하여 미생물 고정화 전 후의 담체를 대상으로 SEM/EDX (Scanning Electron Microscopy/Energy Dispersive X-ray Analyser)를 분석한 결과, C와 N의 성분이 각각 58.5%와 3.85%에서 70.75%와 7.69%로 증가하였다. 결론적으로 미생물 고정화 담체를 적용할 경우 높은 처리효율을 얻을 수 있고 처리시설 규모를 줄일 수 있는 장점을 확인하였다. This study investigated characteristics of nitrification/denitrification rate using PVA (Polyvinyl Alcohol) gel immobilized with specific microorganisms. The media for nitrification and denitrification were producted by immobilizing nitrifying microorganisms (Nitrosomonas europaea and Nitrobacter spp.) and denitrifying microorganisms (Psuedomonas, Bacillus and Paracoccus), respectively. When the media filling rate was the 15% (v/v), the microorganism - immobilized media indicated optimum efficiency. The nitrification rate was $12.53mg\;{NH_4}^+-N/L{\cdot}hr$ and the denitrification rate was $20.37mg\;{NO_3}^--N/L{\cdot}hr$, which was faster than the conventional reaction rate (nitrification: $3.93mg\;{NH_4}^+-N/L{\cdot}hr$). In order to confirm microbial immobilization, SEM/EDX (Scanning Electron Microscopy/Energy Dispersive X-ray Analyser) analysis of media before and after immobilization was conducted. It showed that the contents of C and N increased from 58.5% and 3.85% to 70.75% and 7.69%, respectively. These results suggest that application of microorganism immobilized media improves treatment efficiency and can also reduce the size of the treatment facility.
신동철 ( Shin Dong-chul ),김명주 ( Kim Myeong-ju ),이진우 ( Lee Jin-woo ) 동신대학교 2023 창의융복합연구 Vol.1 No.1
Until now, the analysis of the operational effect of the actuated signal system has mainly used microscopic simulation techniques on national roads. However, recently, local governments have been actively implementing online real-time signal systems, introducing left-turn and pedestrian actuated signal systems, and establishing smart intersection systems. Therefore, the need to analyze the effectiveness of actuated signal system applied in downtown areas has increased, in order to select suitable target locations based on local characteristics and to devise efficient operational strategies. In this study, the operation effect of six intersections in the downtown area of Suncheon City that are currently operating under a actuated signal system was analyzed from the perspective of the operators. To enable quick comparison and judgment of the effects, the ratio of cycle in which the green time increases in the main phase and the degree of increase in green time were analyzed using traffic signal TOD (Time of Day) data and the actual actuated signal database of the Traffic Control Center. To analyze the difference in effects based on the type of actuated signal control, the six intersections were classified into three types: left-turn actuation, simultaneous left-turn and pedestrian actuation, and pedestrian actuation only. The difference in effects by time of day was also analyzed by dividing the daily time periods into five TOD (Time of Day) groups. Through this analysis, it was confirmed that the effectiveness of traffic operation improvement by traffic Actuated Signals was more influenced by the traffic conditions and the time of day than by the control method. The ratio of periods with applied traffic-responsive signals was 46.7% during commuting hours, and 86.2% during nighttime and late night hours. The green time converted to main arterial traffic flow during the commuting hours was an average of 19 seconds per cycle, and during nighttime and late night hours, it was an average of 36 seconds per cycle. Therefore, it is possible to strengthen traffic safety while minimizing the impact on traffic operation by introduction of traffic actuated signal for pedestrian at non-signal intersections in child protection zones and for left-turn vehicles and pedestrians at unprotected left-turn intersections.
광탄성 실험 하이브리드 법에 의한 이축 인장하중비가 모드 Ⅰ균열 선단 응력장에 미치는 영향
신동철(Dong-Chul Shin),남정환(Jeong-Hwan Nam),황재석(Jai-Sug Hawong),Katsuhiko Watanabe 대한기계학회 2006 대한기계학회 춘추학술대회 Vol.2006 No.3
In this study, the photoelastic experimental hybrid method was introduced and applied to the fracture problems of the isotropic polycarbonate plate with a central crack under the uniaxial or various biaxial tensile loadings. And then, the influences of tensile load biaxiality on the crack-tip stress fields, stress intensity factors and photoelastic isocrhomatic fringes were investigated. As the results, we founded that the lateral tensile load applied parallel to the crack in mode-I (changing the load biaxiality) does no influence on the magnitude and distribution shape of σ<SUB>y</SUB>/σ? and τ<SUB>xy</SUB>/σ?, but exert a considerable influence on the shape and slope of isochromatic fringes and the compressive region of σ<SUB>x</SUB>/σ?. And we confirmed that the value of stress intensity factor, K<SUB>I</SUB>, is little changed with increasing the biaxial load ratio (P<SUB>x</SUB>/P<SUB>v</SUB>).
광탄성 실험 하이브리드 법에 의한 이축 인장하중이 균열 선단장에 미치는 영향
신동철(Dong-Chul Shin),남정환(Jeong-Hwan Nam),황재석(Jai-Sug Hawong),Akira SHIMAMOTO,Katsuhiko WATANABE 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.5
In this study, the hybrid method for photoelasticity was introduced and applied to the fracture problems of the isotropic polycarbonate plate with a central crack under uniaxial or biaxial tensile loadings. And then, the influences of tensile load biaxiality on the crack-tip stress fields, stress intensity factors and the shape of isocrhomatic fringe patterns were investigated.
황칠나무, 산수유, 구기자 복합 초임계유체추출물의 항산화 및 항노화 효과
신동철 ( Dong Chul Shin ),김귀철 ( Gwui Cheol Kim ),송시영 ( Si Young Song ),김희진 ( Hee Jin Kim ),양재찬 ( Jae Chan Yang ),김보애 ( Bo Ae Kim ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.6
Objectives: The purpose of this study was to investigate antiaging and antioxidant effects on cultured human skin fibroblast with supercritical fluid extracts of Dendropanax morbifera, Corni fructus and Lycii Fructus. Methods : Supercritical fluid extraction (SFE) technique was applied to extract from three medicinal plants including stem of Dendropanax morbifera, Corni fructus and Lycii Fructus. Antioxidant activity of extract was evaluated by two different assays as 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and super oxide dismutase (SOD) like activities. These extracts were tested for cell viability on HS68 skin fibroblast by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. We investigated the effects of Ultraviolet-B irradiation on cytotoxicity, type 1 collagen, elastin level and oxidative damage in cultured human skin fibroblast (HS68). Recently, many studies have reported that elastin is also involved in inhibiting or repairing wrinkle formation, although collagen is a major factor in the skin wrinkle formation. Results : The extracts obtained dose-dependently increased the scavenging activity on DPPH radical scavenging activity and SOD like activity. The supercritical fluid extracts of complex herbal medicine showed low cytotoxicity as more than 100% cell viability in 100ppm/ml concentration. HS68 fibroblasts were survived 70% at 120 mJ/cm2 UVB irradiation and treated tumor necrosis factor (TNF)-alpha. The levels of aging factors and cytotoxicity were decreased by supercritical fluid extract of complex herbal medicine. Conclusions : These results suggest that supercritical fluid extracts may have value as the potential antioxidant and antiaging medicinal plant.
X - Ray 검사 시스템에서 Image Intensifier의 왜곡 보정
신동철(Shin Dong Chul),오춘석(Oh Choon Suk) 한국정보처리학회 1998 정보처리학회논문지 Vol.5 No.4
Non-destructive x-ray inspection system needs image intensifier to obtain the x-ray image. Captured image from image intensifier is distorted because the input plate of image intensifier has a spherical surface. In the research, in order to compensate this image distortion, we create the model of image intensifier and show mathematically that the image distortion was compensated. To show the performance of the proposed method, experiment was performed in real x-ray inspection system.