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도계장 유래 닭고기와 부산물 및 환경재료에서 Listeria spp의 분리 및 분리균의 특성 II. 분리한 L monocytogenes의 혈청형과 항균제에 대한 감수성
손원근,강호조,Son, Won-geun,Kang, Ho-jo 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.3
To investigate the epidemiological aspects of listeriosis, serotypes of L monocytogenes and antimicrobial susceptibilities of Listeria spp isolated from chicken carcases and chicken slaughter house environmental specimens were determined. Of 28 L monocytogenes strains, 12 strains(42.9%) were serotype 4, and the remaining 16 strains were untypable. Peak distributions of minimum inhibitory concentration$({\mu}g/ml)$ of the isolates were $0.78{\mu}g/ml$ for ampicillin, $0.39{\mu}g/ml$ for erythromycin and penicillin G, $1.56{\mu}g/ml$ for tetracycline and $6.25{\mu}g/ml$ or $12.5{\mu}g/ml$ for chloramphenicol, and $3.13{\mu}g/ml$ to > $100{\mu}g/ml$ for kanamycin and neomycin. Most of 214 isolates were sensitive to ampicillin and erythromycin, but 20. 1~78. 0% of the strains were resistant to tetracycline, kanamycin, penicillin-G and neomycin. Single or double drug resistance were observed in 75.8% of the resistant strains. The most common resistance patterns were Nm P-G(37.4%) in double pattern and P-G(23.7%) in single pattern.
도계장 유래 닭고기와 부산물 및 환경재료에서 Listeria spp의 분리 및 분리균의 특성 I. Listeria spp의 분리
손원근,강호조,Son, Won-geun,Kang, Ho-jo 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.3
To investigate the epidemiological trait of listeriosis, Listeria spp were isolated from poultry meat, products and environmental specimens in chicken slaughterhouse. Also determined were isolation rates by the different enrichment procedures, the biochemical properties of isolates. In a total of 307 samples including poultry meat, liver, feathers, feces, chiller water, scalding water overflow and slaughterhouse floor, Listeria spp were isolated predominantly from scalding water overflow (90.0%), body skin before washing (66.7%), liver (20.0%) and feathers(15.0%) However, few Listeria spp were isolated from body skin after washing and feces. The higher isolation rates were obtained in the secondary enrichment procedure (7.2%) than in the primary enrichment (3.9%); after stored the secondary enrichment cultures for 2 weeks at $4^{\circ}C$, Listeria spp were present in 9.8%. The majority of the isolated Listeria spp were identical to those of the standards strains in biochemical and cultural properties. Overall, Listeria spp were present in 13.4% of the specimens tested, and were in order of prevalence of L innocua(11.1%), L monocytogenes(3.3%), L grayi(0.7%) and L murrayi(0.3%).
Escherichia coli O157:H7 Intimin의 Expression 및 C-terminal 부위의 특성
손원근,Gannon, V. P. J. 한국수의공중보건학회 2001 예방수의학회지 Vol.25 No.3
Portions of the intimin genes of Escherichia coli O157:H7 strain 319 and of the enteropathogenic E. coli O127:H6 strain E2348/69 were amplified by PCR and cloned into pET-28a (+) expression vectors. The entire 934 aa of E. coli O157:H7 intimin, the C-terminal 306 aa of E. coli O157:H7 intimin, and the C-terminal 311 aa of E. coli O127:H6 intimin were expressed as protein fusions with a six histidine residue tag (His-tag) in pET-28a (+). Rabbit antisera raised against the six His-tag 3' end one-third portion of E. coli O157:H7 intimin protein fusion reacted strongly in Western blots with original antigen and its homology protein, the six His-tag-fusioned protein containing full-length intimin of E. coli O157:H7, while reacting weakly with the his-fusioned intimin from enteropathogenic E. coli serotype O127:H6. In contrast with the antiseraraised against the sis His-tag 3'end portion of E. coli O127:H6 recognized only the original antigen, His-intO127C.