동물매개치료가 자폐스펙트럼장애아동의 대인관계, 언어 및 행동에 미치는 사례연구

정순미(Jung, Soon-Mi),서병부(Seo, Byoung-Boo),김충희(Kim, Chung-Hui) 한국보건기초의학회 2020 한국보건기초의학회지 Vol.13 No.2

This study is a single case study that analyzed the effects of animal-assisted therapy (AAT) on a 5-years-old male child diagnosed with autism spectrum disorder class II. To verify the analysis, the Korean Childhood Autism Rating Scale Second Edition (K-SCAR 2), which measures the degree of autism, and the EHWA – Check List for Autistic Children (E-CLAC) scale, which is a behavioral development evaluation scale, were used. The AAT program consisted of a total of 12 sessions, for 50 minutes per session, and was carried out once a week for 12 weeks. As a result of the therapy, the autistic child showed increases in the frequency of word use along with increased language use and behavioral interactions. A K-SCAR 2 test was carried out and the results indicated that the child’s autistic condition had improved from severe autism to moderate autism bordering on minor autism. The E-CLAC indicated that the ability of the child to socially interact had improved as his human relationships were improved and the frequency of language use had increased. In conclusion, the results of this experiment indicated that when AAT was implemented with a child with autism spectrum disorder, the level of autism was lowered, while his human relationships improved as well as his language and use behavioral interactions.

난관체류시간에 따른 생쥐초기배의 체외발생능력

송해범,서병부,김광식,박성은,이상호,Song, H.B.,Seo, B.B.,Kim, K.S.,Park, S.E.,Lee, S.H. 대한생식의학회 1992 Clinical and Experimental Reproductive Medicine Vol.19 No.2

Development in vitro of 2-cell mouse embryos was examined after appropriate exposure to oviductal milieu to demonstrate biological activity present in the oviducts. ICR and ($C57Bl/6{\times}Balb/c$) $F_1$ hybrid mice were superovulated and mated for the recovery of early embryos. Embryos were recoverd at every 2h intervals from 32h post-hCG(hph) to 56 hph. The proportions of developmental stages were determined in the recovered embryos. Development in vitro of 2-cell embryos was more rapid in $F_1$ hybrid than in ICR, showing high proportions of 4-cell embryo and blastocyst at 120 hph. 100% of blastocyst development was obtained at 38hph in $F_1$ hybrid and at 50 hph in ICR when 2-cell embryos were cultured upto 120hph in vitro. Moreover, in vitro culture of oviducts containing 2-cell embryos in ICR mice for 12h from 34hph to 46hph increased developmental capacity of ICR mouse embryo in vitro. The results indicate that oviductal environment contains substances having mitogenic activity and overcoming early cell block in vitro. The mitogenic activity is effective in vitro as well as in vivo.

한우 수정란이식에 의한 쌍자 생산

이준영(Jun Young Lee),정연길(Yun Gil Jung),서병부(Byoung Boo Seo) 한국동물보건학회 2023 한국동물보건학회지 Vol.2 No.3

한우의 체외수정란으로 생산된 육우의 경제성

이준영(Jun Young Lee),정연길(Yun Gil Jung),김수진(Su Jin Kim),서병부(Byoung Boo Seo) 한국동물보건학회 2023 한국동물보건학회지 Vol.2 No.4

This study seeks to compare the economic feasibility and slaughter weight of beef cattle produced based on in vitro embryo. To this end, the study compared and investigated the grade rate of carcasses based on embryo transfer and artificial insemination; slaughter weight, carcass grading results, meat quality and quantity. With respect to the meat grades of carcass based on embryo transfer and artificial insemination; 50.0% (5/10) of embryo transfer-based carcasses were graded with 1++; and 50.0% (5/10), 1+, indicating that 100% (10/10) received at least 1+ grade. On the other hand, artificial insemination-based castrated cattle had no 1++ grade while 30.0% (6/20) received 1+; 60.0% (12/20), 1; and 10.0% (2/20) 2. This finding showed that embryo-based castrated cattle had higher grades than artificial insemination-based cattle as 100% of the former were graded with 1+ or higher. This study also investigated 10 embryo transfer-based castrated cattle and 20 artificial insemination-based cattle to compare their dressed weight, back fat thickness, sirloin cross section, and meat quantity indication. As a result, the embryo transfer-based castrated cattle showed slaughter weight of 488.4kg on average, heavier by 40.6kg than 447.8kg of artificial insemination-based cattle. Back fat thickness was 15.6㎝ on average in embryo transfer-based cases, 0.9㎝ thinner than 16.5㎝ of artificial insemination-based cases. Sirloin cross section of embryo transfer-based cattle was 99.6㎠ on average, 9.8㎠ wider than 89.8㎠ of artificial insemination cases. Meat quantity indication was found 2.1% higher in the embryo transfer cases (62.8%) than artificial insemination cases (64.9%). With respect to meat quantity grade, 10.0% (2/20) of the artificial insemination cases had Grade A; 50.0% (10/20), Grade B; and 40.0% (8/20), Grade C. Embryo transfer-based cattle showed no Grade A among the 10 carcasses; Grade B in 60.0% (6/10); and Grade C in 40.0% (4/10). Comparing the results of economic profitability in terms of carcass auction prices, the embryo transfer-based castrated cattle was KRW 1,119,453 higher than artificial insemination-based castrated case. Per-head economic gain of embryo transfer-based castrated cattle was KRW 2,282,602, higher than KRW 1,567,149 of artificial insemination-based cattle. Based on the findings, this study examined embryo transfer-based castrated cattle and artificial insemination-based cattle to compare their carcass grade rate, dressed weight, grading result and economic feasibility. Consequentially, this study found that in vitro embryo transfer-based castrated cattle had higher meat quality grade, slaughter weight and economic feasibility. Based on information on pedigree, attribute, and carcass data of individual with blood tie which indicate such meat productivity; high grade cattle genetic resources would be secured to contribute to quality meat production. Subsequent study will need to research industrialization based on in vitro embryo transfer.

돼지 종돈, 사료 및 시설에 따른 정육율 및 도체특성 조사

장세헌,임원호,서명인,소병헌,노이현,박진목,서병부,Jang, Se-Heon,Lim, Won-Ho,Seo, Myung-In,So, Byung-Hun,Noh, Yi-Hyun,Park, Jin-Mok,Seo, Byoung Boo 한국축산식품학회 2018 축산식품과학과 산업 Vol.7 No.1

서로 다른 배양액의 조건이 우수한 등급의 한우의 난소에서 채란한 난포란의 체외수정란의 생산에 미치는 영향

이준영 ( Jun Young Lee ),정연길 ( Yun Gil Jung ),서병부 ( Byoung Boo Seo ) 한국동물자원과학회(구 한국축산학회) 2016 축산기술과 산업 Vol.7 No.1

서로 다른 배양액의 조건이 우수한 등급의 한우의 난소에서 채란한 난포란의 체외 수정란 생산에 미치는 영향에 관하여 실험을 실시하였다. 한우 난자 수에 대한 2세포 난할율은 대조구 86.7%, IVDM 101 배양액 92.9% 그리고 IVD101 배양액 90.1%로 나타났다. 이러한 결과에서 보는 것과 같이 IVDM 101 배양액와 IVD101 배양액이 대조구 보다 높은 결과를 보였으며, IVDM 101 배양액이 IVD101 배양액 보다도 더 좋은 결과를 나타났다. 한우의 체외배양 배반포 배의 발생율은 대조구에서 12.4%, 혈청이 첨가되지 않은 IVMD 101 배양액에서 38.4% 그리고 IVD 101 배양액에서 32.4%로 대조구 보다 매우 높은 결과를 얻었다. 또한 탈출 배반포 배는 대조구, IVMD 101 배양액과 IVD 101 배양액에서의 각각 5.3%, 33.9%, 그리고 28.6%의 결과를 얻었다. 탈출 배반포 배에서는 IVMD 101배양액과 IVD 101 배양액이 대조구 보다 매우 높은 결과를 보였다. 한우 배반포 배의 평균 세포수는 TCM-199+10% FBS 배양액, IVMD 101 배양액 그리고 IVD 101 배양액에서 각각 128.3개, 165.7개 그리고 163.6개였다. 이 결과에서 보는 것과 같이 IVMD 101 배양액 그리고 IVD 101 배양액에서의 세포수가 대조군 보다 매우많았다. 수정란 이숙한 후 소의 임신율은 무혈청 배양액 (39.6%)이 혈청 첨가 배양액 (32.8%) 보다 높은 결과를 보였다. 또한 사산율은 무혈청 배양액 (4.9%)이 혈청 첨가 배양액 (13.6%) 보다 낮은 결과를 보였다. 이상의 결과를 종합해 보면 무혈청 배양액인 IVMD 101 배양액과 IVD 101 배양액이 혈청이 첨가된 배양액 보다수정란의 배 발달율, 배반포배의 세포수 그리고 임신율에 있어서 보다 우수한 결과를 나타냈으며, 특히 IVMD 101배양액이 더 좋은 결과를 보여 이 배양액을 사용하는 것이 매우 효율적이라고 판단된다.

수컷 자바리 Epinephelus bruneus에 있어서 hCG 투여에 따른 혈중 성호르몬 및 정자 활성도 변화

김효원(Hyo-Won KIM),김정현(Jung-Hyun KIM),박진우(Jin Woo PARK),정민환(Min Hwan JEONG),지승철(Seung-Chul JI),김재훈(Jae-Hoon KIM),서병부(Byoung Boo SEO),김대중(Dae-Jung KIM) 한국수산해양교육학회 2021 水産海洋敎育硏究 Vol.33 No.4

농산부산물 발효사료 급여가 비육흑염소의 생산성 및 영양소 이용율에 미치는 영향

최순호(Sun Ho Choi),황보순(Soon Hwangbo),김상우(Sang Woo Kim),조영무(Young Moo Cho),유용희(Yeoung Hee Yoo),김태일(Tae Il Kim),김맹중(Maeong Joung Kim),이상민(Sang Min Lee),최창원(Chang Weon Choi),서병부(Byeong Bu Seo),조익환(Ik Hwan 한국초지조사료학회 2012 한국초지조사료학회지 Vol.32 No.1

This study was conducted to investigate the feeding fermented agro-industrial by-products on estimating the productivity and nutrient utilization of fattening black goats. Four experimental groups were set up with each of 6 animals for a 30 days feeding trial on growth performance and 12 black goats on nutrient digestibility were allocated to 4 groups of 3 animals each by latin-square design, control (offered concentrate and rice straw) and 3 treatment groups (offered fermentation feed and rice straw). Average daily gain (ADG) showed a significant high in control group, T3 was lower than other groups (p<0.05). DM and OM intake (㎏/head) were 718.8, 680.9 g and 634.2~699.2 g, 602.8~660.4 g for control and treatment groups, respectively. As increasing of rice bran content was decreased feed intake. Digestibility of DM and OM for control group were significantly higher than treatment groups (p<0.05), and T3 treatment group with 30% rice bran was the lowest among treatment groups. Nitrogen retention was significantly higher in control and T1 groups. Thus, these results showed that productivity and nutrient utilization were similar between fermentation feeds with agricultural by-products and commercial feeds. However, this study was suggested that it must contain less than 5.0% crude fat because of feed efficient was decreased by increasing of rice bran content. 본 연구는 농산부산물을 이용한 발효사료의 급여효과를 알아보기 위해 비육 흑염소의 생산성 및 영양소 이용률을 조사하였다. 증체조사를 위한 사양시험은 거세흑염소 24두를 이용하여 처리구당 6두씩 30일 수행하였고, 소화율시험은 거세흑염소 12두를 공시하여 처리구당 3두씩 라틴방각법으로 실시하여, 처리구는 대조구(배합사료 및 볏짚 급여구)와 3개의 시험구(발효사료와 볏짚 급여구)로 각각 배치하였다. 일당증체량은 대조구가 가장 높았고, T3구가 가장 낮았다(p<0.05). 1일 두당 건물섭취량과 유기물섭취량은 시판사료를 급여한 대조구가 각각 718.8과 680.9 g, 농산부산물 첨가구가 각각 634.2~699.2와 602.8~660.4 g으로 나타나 농산부산물 중 미강 첨가구가 높을수록 섭취량이 낮아지는 경향으로 나타났다. 건물과 유기물 소화율은 대조구가 농산부산물 첨가구보다 유의하게 높았고(p<0.05). 시험구간에는 미강을 30% 첨가한 T3구가 가장 낮았다(p<0.05). 질소 축적률은 대조구와 T1구가 유의하게 높았다. 따라서, 본 연구의 결과 농산부산물을 이용한 발효사료(T1과 T2구)는 시판사료를 급여한 대조구와 비교 시 증체는 86~90%, 건물소화율은 91.7~93.1%의 수준을 보였으며, 질소이용성은 대등한 수준을 보였다. 그러나 미강의 첨가 비율이 높아질수록 사료효율 저하가 우려되므로 조지방 함량을 5.0% 이하로 배합하는 것이 필요하다 판단된다.

Mouse의 연령과 체중이 배란율, 핵성숙도 및 체외수정에 미치는 영향

박기상,서병부,송해범 大邱大學校出版部 (대구대학, 한사대학 1992 農業科學硏究論文集 Vol.6 No.-

This research was conducted to investigate the interrelationship among age and body weight of mice to the number of ovulated eggs, percentage of matured oocytes and in vitro fertilization using out-bred ICR mice. The results obtained are as folows, 1) The number of ovulated eggs was 40±8~42±7, percentage of MII was 75~76% and in vitro fertilization rate was 79~81% in 3~6 weeks age of mice. 2) The number of ovulated eggs were 39±8 and 41±7, percentages of MII were 75 and 76% and in vitro fertilization rates were 81 and 80% in 15 and 25g weight of mice, respectively.