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이승연,변현재,최희락,원종인,한정훈,박성수,김동현,성종환 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.2
Gut microbiota dysbiosis and intestinal epithelial cell barrier dysfunction are associated with diseases such as inflammatory bowel disease and type 2 diabetes. To study the interaction of gut microbes and intestinal epithelial cells, in vitro model systems for co-culturing intestinal cells and gut microbes are essential. Since most gut microorganisms are anaerobic bacteria, it is difficult to co-culture microbes with intestinal epithelial cells that require oxygen during culture. We have developed a novel gut microbialepithelial cell (GMEC) co-culture system, a microfluidic chip in an anaerobic chamber with oxygen gradient within the chip. Appropriate co-culture flow rates were determined based on the experimental observation as well as fluid dynamics and molecular transport simulations. Using GMEC system, Escherichia coli and Lactobacillus plantarum were co-cultured with gut epithelial cell line, Caco-2. We expect that our GMEC co-culture system can potentially be a useful platform for studying the interaction between intestinal epithelial cells and gut microbes.