http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
박청율,백다솜,오종희,최종윤,배대현,김정선,장길훈,이수헌 한국식물병리학회 2016 식물병연구 Vol.22 No.2
Cymbidium mosaic virus (CymMV) is a major virus infecting orchid plants and causing economic loss. In this study, the incidence of viral infection in Calanthe spp. at the Korean Institute of Calanthe was investigated using reverse transcription polymerase chain reaction. The CymMV infection rate was 42%, and the two viruses Odontoglossum ringspot virus and Cucumber mosaic virus had frequencies of 8% and 2%, respectively. Additionally, we characterized an isolate of CymMV, CymMV-GW, using biological tests and examined the nucleotide sequence properties of its complete genome. CymMV-GW induced chlorotic ringspots and chlorotic spot symptoms in inoculated leaves of Chenopodium amaranticolor and Nicotiana benthamiana, respectively. In this study, we have for the ἀrst complete genome sequence of CymMV-GW in Korea. The CymMV-GW genome was 6,225 nucleotides in length, excluding the poly-(A) tail, and showed whole-genome nucleotide and amino acid sequence identities of 97.7% and 100%, respectively, with the NJ-1 isolate of CymMV. Here, we report the complete genome sequence of the CymMV-GW isolate and viral infection rates for Calanthe spp. in Korea.
국내 브루그만시아에서 분리한 Brugmansia mosaic virus의 특성
박충열,김봉섭,남문,이민아,백다솜,배양수,박은혜,김정선,최종윤,임승모,문제선,이수헌 한국식물병리학회 2014 식물병연구 Vol.20 No.4
In May 2013, an angel’s trumpet leaves showing mosaic and malformation symptoms were collected from Suwon city, Gyeonggi-do. An analysis of the collected sample by transmission electron microscopy observation showed filamentous rod particles of 720-800 nm in length. On the basis of the these observations, we performed PCR against three reported Potyviruses (Brugmansia mosaic virus, Colombian datura virus andBrugmansia suaveolens mottle virus), and the sample was positive for BruMV. Pathogenicity and host rangetest of BruMV was determined by mechanical inoculation. Solanaceae (tobacco, tomato and eggplant) andAmaranthaceae (ground cherry) appeared typical virus symptoms. To determine coat protein of this virus,we designed specific primer pairs, and performed PCR amplification, cloning, and sequencing. Phylogeneticanalysis showed that BruMV-SW was most closely related to BruMV isolate SK. Comparison of the BruMV-SWcoat protein nucleotide sequences showed 92% to 99% identities to the other BruMV isolates. In May 2013, an angel’s trumpet leaves showing mosaic and malformation symptoms were collected fromSuwon city, Gyeonggi-do. An analysis of the collected sample by transmission electron microscopy observationshowed filamentous rod particles of 720-800 nm in length. On the basis of the these observations, we performed PCR against three reported Potyviruses (Brugmansia mosaic virus, Colombian datura virus and Brugmansia suaveolens mottle virus), and the sample was positive for BruMV. Pathogenicity and host range test of BruMV was determined by mechanical inoculation. Solanaceae (tobacco, tomato and eggplant) and Amaranthaceae (ground cherry) appeared typical virus symptoms. To determine coat protein of this virus, we designed specific primer pairs, and performed PCR amplification, cloning, and sequencing. Phylogenetic analysis showed that BruMV-SW was most closely related to BruMV isolate SK. Comparison of the BruMV-SW coat protein nucleotide sequences showed 92% to 99% identities to the other BruMV isolates.