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백경훈,박성권,이명훈,김성일,조수현,최창본 한국축산식품학회 2014 한국축산식품학회지 Vol.34 No.3
This study was performed to develop a rapid immuno-assay kit, by using a specific antigen to detect Hanwoo brand meat. We selected a synthetic antigen specific to our target antibody, named BIO-TAG (Tyr-D-Ala-Phe), by utilizing a computer-based analysis and literature review. BIO-TAG tagged with adjuvant was subcutaneously injected in sheep and Hanwoo. The serum and meat juice of the immunized or non-immunized animal were then analyzed, to measure the titer of antibodyby ELISA and Western blot. The amount of antibodies against the BIO-TAG increased (p<0.05) in serum by vaccination. Furthermore, meat juice from the immunized Hanwoo showed greater (p<0.05) antibody titer, compared with those fromnon-immunized groups. To optimze the dilution factor, we performed dot-ELISA, with various combination levels of BIO-TAG. Results from dot-ELISA showed that 2 mg/mL BIO-TAG was sufficient to distinguish the immunized meat from non-immunized groups. These results support our hypothesis that simple immunization of Hanwoo generates a sufficient amountof antibodies to be detectable in the meat juice by means of the immune-assay. Therefore, specific Hanwoo brand meat canbe more precisely identified by our rapid diagnostic kit. This technology can deter possible fraud of counterfeit meat brandsin the Korean domestic market with ease and rapidity; and offers a new tool that guarantees consumers high quality Hanwoobrand beef.
백경훈 부산연구원 2018 연구보고서 정책연구 Vol.2018 No.0
The distortion of the urban water cycle has caused many issues including urban flooding, groundwater depletion, drying streams, stream quality decline, etc. To prevent and solve these problems, the central and some local governments have been formulating various policies and projects such as rainwater penetration and pooling, water reuse, low-impact development (LID), and more. Unlike Seoul or Suwon, however, Busan doesn’t make the grade when it comes to pushing ahead plans for maximizing the water cycle especially using rain water. Since 2010, the impervious surfaces in Busan cover 197.6 - 25.4 percent of the whole ㎢ area. When forests and natural water sources/accumulations are excluded, imperviousness is 47.5 percent, which means about half of the available city land is impervious. Particularly in the Oncheoncheon Stream basin, the impervious surfaces add up to 25,610㎢ or 45.1% of this area. Excluding forests and water sources/accumulations, the imperviousness in this region is 79%, indicating most of its available land is impervious. To restore the water cycle in Busan, this study recommended action plans that could be broken down to three major parts: preparing institutional strategies; building an infrastructure to implement these tactics; and establishing public-private partnerships. First, for the institutional strategies, this study suggested a management plan for the urban water cycle, enactment of relevant guidelines, construction of green infrastructure, etc. Second, prior consultation for LID and integrated administration systems based on cooperation should be the foundations to realize this objective. Finally, to promote private participation, it is required to offer education, public relations and incentives to join and strengthen governance for water cycle policies in which citizens, experts and the departments concerned participate together.