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넙치 전 암컷 집단의 생산을 위한 연구 II. 성전환된 자성발생성 2배체 수컷의 자손 검정
방인철,김경길,김윤,김종현,Bang In-Chul,Kim Kyung-Kil,Kim Yoon,Kim Jong-Hyun 한국양식학회 1996 韓國養殖學會誌 Vol.9 No.2
본 연구는 성전환된 자성발생성 2배체 수컷의 정액을 보통 암컷 및 자성발생성 2배체 암컷의 성숙란에 인공수정시킨 후 자손의 성비를 분석하였다. 자성발생성 수컷의 유도는 자성발생성 2배체를 전장 13.5 mm에서 57.6 mm까지 62일간 $17{\alpha}$-methyltestosterone 10 ppb의 농도로 침지 처리하여 유도하였다. 자손 검정 결과, 보통 암컷과 자성발생성 수컷 교배군의 암컷 유도율은 평균 $37.4\%$로 낮게 나타났다. 그러나 자성발생성 2배체 암컷과 자성발생성 수컷 교배군에서는 8개 실험군 중 6개 실험군에서 $100\%$ 암컷이 유도 되었고 2개 실험군에서는 $96.9\%$ 및 $95\%$ 암컷이 유도되어, 전체 $99\%$ 의 암컷 유도율을 나타내었다. 이러한 결과는 자성발생성 2배체 암컷과 성전환된 자성발생성 2배체 수컷이 정상적인 생식 기능을 통해 교배되어 전 암컷 집단을 생산할 수 있음을 시사한다. A study was carried out to test sex ratio of the progeny that were induced by the artificial fertilization between normal female and gynogenetic diploid eggs and sex-reversed gynogenetic diploid male sperm. Sex reversed gynogenetic diploid males were produced successfully in gynogenetic diploid female by immersion treatment with $17{\alpha}$-methyltestosterone at the concentration of 10 pub from $13.5{\pm}0.22$ mm to $57.6{\pm}5.1$ mm in total length. In $F_1$ progeny test, matings between normal females and sex-reversed gynogenetic males were not produced all female, but matings between gynogenetic diploid females and sex-reversed gynogenetic males were produced all female populations in six out of eight groups and two lots produced $96.9\%$ and $95\%$ female. These data showed that all-female populations were produced by crossing between gynogenetic diploid female and sex reversed gynogenetic diploid male.
멸종위기어류 미호종개 Cobitis choii (Pisces: Cobitidae)의 분포양상 및 서식개체수 추정
방인철 ( In Chul Bang ),이일로 ( Il Ro Lee ),고명훈 ( Myeong Hun Ko ) 한국어류학회 2012 韓國魚類學會誌 Vol.24 No.1
멸종위기 어류 미호종개의 분포양상을 조사하고 대표서 식지의 서식개체수를 추정하였다. 과거 (1984~2004)에는 20개 지점에서 출현하였으나 현재 (2006~2011)는 6개 지 점에서만 서식이 확인되어 분포지역이 급격히 감소한 것으로 추정된다. 출현지역은 대부분 모래로 이루어진 하천 하류부였으며 서식구간은 매우 협소하였다. 대표서식지인 백곡천과 지천의 서식개체수를 표지-재포획법 등으로 추정한 결과 백곡천은 9,106개체, 지천은 34,717개체가 서식하는 것으로 추정되었다. The distribution status and population size of the endangered species, Cobitis choii, were investigated in the Guem River, Korea. From 1984~2004, this species occurred at 20 survey stations; however, their numbers have but rapidly decreased in recent years (2006~2011), during which time they were only observed at six stations. The area of their habitat is narrow and occurs in primarily downstream, sandy zones. Using the mark-recapture method, populations were estimated at 9,106 and 34,717 individuals for the main habitats of Baekgok and Ji Stream, respectively.
방인철(In Chul Bang),남윤권(Yoon Kwon Nam),노충환(Noh Choong Hwan),박준택(Joon Taek Park),한경호(Kyoung Ho Han) 한국수산과학회 2001 한국수산과학회지 Vol.34 No.1
한국산 꺽지과 어류 3종에 대한 세포유전학적 기초 자료를 얻기 위하여 염색체 핵형, 적혈구 세포 및 핵의 크기와 DNA 함량을 조사하였다 꺽지 (Coreoperca herzi), 꺽저기 (C. kawamebari) 및 쏘가리 (Siniperca schezeri)의 핵형은 각각 2n=48 (4SM+44A,T), 2n=48(6SM+42A,T) 2n=48(4SM+44A,T) 이었으며, 암·수간 heteromorphic한 성염색체는 발견할 수 없었다. 쏘가리의 적혈구 세포 및 핵의 표면적과 체적은 다른 2종보다 작았다. DNA 함량은 적혈구 세포 크기에서 같은 경향을 보여, 쏘가리가 1.47 Pg/cell로 꺽지 (1.83 pg/cell) 및 꺽저기 (1.85pg/cell)보다 유의하게 작았다. Cytogenetic characteristics of three species in Centropomidae (Coreoperca herzi, C. kawamebari and Siniperca schezeri) were evaluated, based on karyologcial analysis, erythrocytic measurement and genome size estimation using flow cytometry. Modal chromosome number of three species was same as 2n=48. Karyotypes were 4SM+44A·T (NF=52) for Coreoperca herzi, 6SM+42 A·T (NF=52) for C. kawamebari and 4SM+44A·T (NF=52) for Siniperca schezeri. Heteromorphic sex chromosome was not found in both sexes of any species examined. Cellular and nuclear volumes of Siniperca schezeri were smaller than those of other two species. Average amounts of cellular DNA contents estimated by flow cytometry were well coincided with erythrocytic sizes. The estimated genome sizes were 1.83, 1.85 and 1.44 pg/cell for C. herzi, C. kawamebari and S. schezeri, respectively.
참종개 Iksookimia koreensis의 난발생 및 초기생활사
방인철 ( In Chul Bang ) 한국하천호수학회 2012 생태와 환경 Vol.45 No.1
In the present study, egg development and early life history of Korean spined loach, Iksookimia koreensis, were observed. Adult fish were sampled using spoon nets in Okgye-ri, Hoengseong-gun, Gangwon-do, Korea in July 2010. Eggs were obtained after injecting females with Ovarprim. Eggs were then artificially fertilized in the laboratory using the dry method. Mature eggs were slightly adhesive and transparent with a light yellowish color, and measured 1.40±0.04mm(mean±SD) in diameter. Hatching of the embryo occurred approximately 50 h after fertilization in the water at 23℃, and newly hatched larvae were averaged 4.7±0.21 mm in total length. 5 days after hatching, the averaged total length of larvae was 7.1±0.25 mm and their yolk sacs had been completely absorbed. 17 days after hatching, fish started to enter the juvenile stage and reached 12.2±1.10 mm in total length. 80 days after hatching, the band patterns and external form of juvenile fish were similar to those of adults, and they averaged 31.0±3.98 mm in total length.