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대장균으로부터 생산된 Bacillus licheniformis WL-12의 Cellulase 특성
박종덕,김연아,윤기홍,Park, Jong-Duk,Kim, Yeon-A,Yoon, Ki-Hong 한국미생물학회 2009 미생물학회지 Vol.45 No.3
Carboxymethyl celluase (cellulase) was purified from cell-free extract of the recombinant Escherichia coli carrying a Bacillus licheniformis WL-12 cellulase gene by DEAE-Sepharose and phenyl-Sepharose column chromatography with specific activity of 163 U/mg protein. The molecular mass of the purified enzyme was estimated to be approximately 49.5 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme had a pH optimum at 5.5 and a temperature optimum at $55^{\circ}C$. The activity of the enzyme was completely inhibited by SDS (5 mM), and slightly enhanced by $Cu^{2+}$ (5 mM). The cellulase was active on CMC, konjac, barely glucan and lichenan, while it did not exhibit activity towards xylan, locust bean gum, and p-nitrophenyl-$\beta$-glucopyranoside. The predominant products resulting from the cellulase hydrolysis were cellobiose and cellotriose for cellooligosaccharides including cellotriose, cellotetraose and cellopentaose. The enzyme could hydrolyze cellooligosaccharides larger than cellobiose.
박종덕,Park, Jong Duk 한국가정간호학회 2016 가정간호학회지 Vol.23 No.2
Purpose: This study aims to classify multiple forms of home care services from a service user's perspective. By comparing the German system to the Korean one, this study seeks to find better ways of enhancing client-centered care. Methods: The data of 121 home care users were collected from six home care centers that had under the management of nursing managers for more than five years. The researcher used a German instrument to estimate the level of care. Results: High correlation was found between German and Korean assessment criteria (r=0.81, p<.001). However, compared to their German counterparts, Korean home care providers allocated more time towards provision of domestic help per daily visit. German home care providers allocated more time towards services relating to provision of support for physical activities, including personal hygiene. It was confirmed that the level of care in Korea does not correlate with either the categories of home care services and duration. Conclusion: Therefore, care services should be offered according to long-term care needs. This study suggests a need for systematic care aimed at strengthening the client's autonomy which should be properly planned, implemented, and managed.
박종덕,최진무,Park, Jong-Duk,Choi, Jin-Mu 대한공간정보학회 2011 한국공간정보학회지 Vol.19 No.1
Recently urban area has suffered from frequent flood event by local heavy rain. This study performed flood tests for the Jungnang river using HEC-RAS model. Based on 1m LiDAR data, river geometry data were produced using HEC-GeoRAS. For 100-year frequency flood, 200-year frequency flood, and PMF, flooding areas were estimated. Ten sub-zones of the entire flooding area were identified based on the nearest refugees and used to analyze evacuation paths to the refugees. The results showed that approximately 70% of flooded area were residential, commercial, and transportation areas so that much loss of life and property could be possible. Path analysis showed that the shortest path distances to refugees were about 1000m average. Evacuation warning given at a proper period could minimize loss of life and property. This study provides the guideline for flood evacuation plan in urban area.
교사의 해석적 실천에 기초한 교육과정 실행 방안: 내러티브적 관점
박종덕 ( Jong Duk Park ) 한국교육과정학회 2011 교육과정연구 Vol.29 No.4
We have made explicit implementation of curriculum in the perspective of objective fidelity, mutual adaptation, and curriculum enactment. Aoki`s ``interpretive praxis`` in his phenomenological-hermeneutical approach shows the importance of teachers` effort in curriculum enactment. But Aoki does not make clear what the teacher should do for his ``interpretive praxis``. However, Bruner`s theory of the narrative focuses on the methodological principles in education. The narrative, in its educational context, is recognized as the curriculum which is reconstructed by the teacher, and it is identical with ``contents`` in the terminology of Marshall McLuhan`s theory of media. A careful scrutiny of McLuhan`s theory reveals that ``contents`` consists of medium and message, respectively the visible and non-visible aspect of contents. Here, the medium is the ``ground`` of contents, and the message the unmanifested standard. The ``pedagogical content knowledge`` as investigated and practiced in the fields of education of subject matters in curriculum can be seen as a typical example of curriculum implementation. It is evident, however, that we can and must place an emphasis on the conceptual nature of ``contents``, most of which can be developed by establishing the internal relation of medium to message. Thus, the theory of narrative and media can give us a useful guide in assessing the extent to which curriculum implementation can be meaningful in the classroom.
박종덕(Jong-Duk Park) 한국도덕교육학회 2021 도덕교육연구 Vol.33 No.1
Dewey’s theory of morality is characterized by attempt to establish the relation of knowledge to morality. From Dewey’s point of view, the nature of moral education must be grasped in the light of the specific context of the subject education which leads us to the acquisition of knowledge. But Dewey’s theory of morality, in spite of these theoretical contributions, limits itself to some points. First of all, Dewey defines morality by way of the useful purpose that is achieved by learning the knowledge. Therefore, Dewey’s morality is not the morality of the studying subject acquired by learning the knowledge, but the morality of the work, meaning morality by which everyone conducts his occupational life. According to The Doctrine of the Mean, the morality of the studying subject, which is quite distinct from the morality of the work, but which is not different from the not-yet-manifested mind, an ingredient essential to the studying subject. Thus Moral education is to make it possible to express the not-yet-manifested mind into daily words, thoughts, and actions by itself. It is the task of moral education that is derived from the nature of moral education. Conventional ethics can be regarded as deserving of the content of moral education in that it also deals with the moral life and its justifications. However, ethics explicate the justifications of the moral life, but it leaves the problem of the acquisition of moral life, which amounts to the internalization of studying subject. This would suffice to differentiate between educational theory and ethics. The more ethics depends on the educational theory which shows the relation of the studying subject to the not-yet-manifested mind, the more sufficiently ethics accomplishes what is most characteristic of it. In other words, ethics needs to be re-established as the educational theory which shows the relation between the internalization of studying subject and the justification of the moral life. Since Neo-Confucianism is the theory of education through which ethics may be completed, we reach the statement that the task of moral education in the future centers around the Neo-Confucianism.
박종덕 ( Jong Duk Park ) 연세대학교 김모임간호학연구소 2006 간호학탐구 Vol.15 No.2
The Korean「care Insurance system for the aged」prescribes the provisions relating to the care benefit such as physical activity or housework assistance offered to the aged who cannot manage their everyday life by themselves because of the advanced age or the geriatric diseases. It is for the purpose of improving the quality of people`s life by promoting the health and the stabilization of life for the aged and lightening the familial burden. Its measure (substitute) is now, December 2006, under discussion in the National Assembly. The benefit recipient is a person aged 65 or older or suffering from a geriatric disease stipulated by the President such as dementia or cerebrovascular diseases. It is stipulated by law that the「care staff」attached to the「care institution」perform the physical activity or housework assistance. The institutions providing the care benefit are classified as day/night care institution, short term care institution, regular hospitalizing institution, home visit care institution that the staff visits the care recipient`s home and provides services. In the second model project, the services provided to the care recipient are classified as above mentioned housework care, bathing care, nursing care, rental of welfare equipment. Regarding the provided services, the basic nursing activity like a housework care and a bathing care offering the physical activity and housework assistance, and the nursing care according to the doctor`s prescription are the principal services of this system. Whatever institution day/night care institution, short term care institution, regular hospitalizing institution, home visit care institution-may provide whatever service, the service should be operated reasonably in consideration of all of the aged`s state of mind and body, his life environment and his and his family`s wants/choice, and within the necessary extent. It is also stipulated the care service should be operated in conjunction with the medical service to avoid making worse the care recipient`s state of mind and body and his health. In other words, in order to execute the benefit principle stipulated by law is indispensable the continuous process of the concrete plan of「care」, that is, 「basic nursing」and「medical nursing」, the offer of services according to that plan, and the evaluation of results of that service. In this writing, I would like to communicate that, nursing being regarded as professional, these times need the role of the professional nurse who applies to the on the spot situation the nursing process of consideration, plan, execution and evaluation. Concerning the introduction of the care insurance system, it is time to frame urgently the policy to settle a system that establish the plan integrating the nursing and the care (basic nursing), entrust the「care staff」with the care (basic nursing) service such as physical activity and housework assistance, and guarantee the care (basic nursing) quality under the nurse`s direction and control.
박종덕(Jong-Duk Park) 한국도덕교육학회 2016 도덕교육연구 Vol.28 No.2
진정한 의미에서의 심리학은 영혼에 관한 탐색을 그 핵심적 관심사로 삼는 영혼심리학이라고 볼 수 있다. 프로이트의 정신분석심리학과 융의 분석심리학은, 약간의 상이한 주장에도 불구하고, 그 방면의 영혼심리학을 예시하고 있다. 특히, 융의 분석심리학은 영혼의 회복 문제를 본격적으로 취급하고 있다는 점에서 주목의 대상이 된다. 다만, 융은, 영혼의 회복은 꿈에 현시되는 비언어적 상징을 분석하는 일을 통하여 가능하다는 점을 강조할 뿐, 그 밖의 경로를 통해서도 가능하다는 점을 간과함으로써 보완의 여지를 남겨놓고 있다. 그리고 그 보완의 방향은 성리학의 심성함양의 매체이론에 비추어 모색될 수 있다. 성리학의 심성함양의 매체이론에 의하면 심성과 그 심리적 대응물로서의 영혼은 비언어적 상징, 언어적 상징, 개념에 의하여 회복된다. 이 세 가지 매체가 영혼을 회복하는 경로가 된다는 점을 밝힐 때에야 비로소 융의 심리학에 예시되어 있는 영혼심리학은 완성된 형태를 갖출 수 있게 된다. 이것은 곧, 영혼심리학은 영혼의 회복을 주된 관심사로 삼아 인간을 신적 경지로 이끄는 교육적 맥락에서 정립될 필요가 있다는 것을 뜻한다. Psychology in a true sense can be described as the psychology of the soul, assuming that psychology should investigate the concept of the soul. Some contrary suggestions notwithstanding, Freud’s psycho-analytical psychology and Jung’s analytical psychology are typical exemplars of the psychology of the soul. Especially, it is worth reminding that Jung’s analytical psychology makes a claim that the soul should be reinstated as its own. However, Jung asserts that the soul can be reinstated only through analysing nonlinguistic symbols of the dream. This is why Jung’s analytical psychology needs to be reinterpreted in terms of Neo-Confucian theory of the media of cultivation of mind. Neo-Confucianism, when interpreted in the light of theory of the media of cultivation of mind, offers the view that ‘the primordial mind’ which is Neo-Confucian equivalent to soul is reinstated by the media of ‘nonlinguistic symbol’, ‘linguistic symbol’, and ‘concept’. Only when we recognize three kinds of media as the ways of reinstatement can we establish the psychology of the soul exemplified by Jung’s analytical psychology. In this sense, we can make sure of placing the psychology of the soul in its true context, which aims to reinstate the soul and to uplift human being to the divine state.
박종덕(Jong-Duk Park) 한국도덕교육학회 2020 도덕교육연구 Vol.32 No.2
The theory of cultivation of mind centers around the acquisition of the not-yet-manifested mind through its manifestations. When the theory of religion is established as an element of the theory of cultivation of mind, it can be the theory of religion in a true sense. According to the Doctrine of the Mean(中庸zhongyong), the theory of religion can be regarded as the theory of the cultivation of mind. In this sense the Doctrine of the Mean itself is the core of the theory of religion. Among the various concepts of the Doctrine of the Mean, the concepts of the Equilibrium(中chung) and the Harmony(和he) reveal the twofold structure in which the not-yet-manifested mind and its manifestations are precisely folded in an inseparable form, and the circularity in which the not-yet-manifested mind can be obtained only through its manifestations, the media, in the word of theory of cultivation of mind. On the other hand, the Ghost(鬼神guishen) is identical with the Noble Man(聖人shengren) who is in pursuit of the Sincerity(誠cheng), the Unity of Heaven and Human(天人合一tienrenhei). But the Ghost, unlike the Noble Man, is God in Mind that activates the driving force of the mind. The reason why humans must take personal posture, not an outsider’s, is that the Ghost, the God in Mind, triggers the movement of the human mind. The metapraxis is constituted with the three explanatory principles such as twofold structure, circularity, and personal posture which are explained in the theory of religion of the Doctrine of the Mean. In that the theory of religion of the Doctrine of the Mean explains the cultivation of mind, it shares common concern with the metapraxis. Viewed from the principles of metapraxis, religion can be defined as the activity of the cultivation of mind which gradually confirms God in Mind, a religious equivalent of the not-yet-manifested mind, through its manifestations. More precisely, it is the linguistic symbol which mediates representational thought that we can recover the ability of God in Mind.