국내 간호사의 직장 내 괴롭힘 경험: 질적 메타 종합연구

박은준,강현욱,고지운 한국간호교육학회 2023 한국간호교육학회지 Vol.29 No.4

Purpose: This study aimed to conduct a qualitative synthesis of workplace bullying experiences among nurses in Republic of Korea. Methods: Following the PRISMA guideline, a literature search was conducted using seven domestic and three international databases. Studies published in Korean or English from inception to December 31, 2022 were included. A meta-aggregation approach suggested by the Joanna Briggs Institute was used to synthesize the research findings. Results: Fourteen studies were included in this review. As a result of a data analysis of the selected studies, 199 subthemes and supporting illustrations were identified and grouped into 36 related categories. Based on the subthemes and categories, five synthesized findings were developed: (1) the individual and organizational causes of workplace bullying; (2) the various types of physical violence and psychological harassment; (3) the negative impact of workplace bullying and its effect on self-growth; (4) active and passive coping efforts in dealing with bullying; and (5) strategies for preventing bullying incidents. Conclusion: Based on the synthesized findings, four recommendations were made: (1) improving the challenging working conditions for nurses; (2) enhancing educational programs for new nursing graduates; and (3) promoting proactive responses from nursing managers in conjunction with an expansion of resilience training for nursing students. Finally, to address the issue of workplace bullying, (4) multi-center and multi-level research involving nursing organizations needs to be conducted.

어류 경구백신 현황과 전망

박은준,김미나,박주영,차재호,정화지,Park, Eun-Joon,Kim, Mi-Na,Park, Ju-Young,Cha, Jae-Ho,Chung, Hwa-Jee 한국식물생명공학회 2010 식물생명공학회지 Vol.37 No.3

As the capture fishing industry has declined, the aquaculture industry has become an important source of seafood. With this tendency all fish farming will be performed by large-scale farms where the fish are cultivated in much high density and as a result the incidence of infectious diseases increases. Therefore, vaccination has become an increasingly important part of aquaculture as a cost effective method of controlling various diseases. The early fish vaccines were the formalin inactivated bacteria or virus cultures, which were administered by either immersion or injection. Recombinant DNA biotechnology allowed us to develop orally administrated DNA and recombinant vaccines. In terms of the manufacturing process and cost, Lemna and Spirodela is the most efficient and reliable plant expression system for the production of edible vaccine.

간호대학생 관련 무례 행동의 연구동향 :텍스트네트워크분석 적용

박은준,박찬숙 인하대학교 교육연구소 2020 교육문화연구 Vol.26 No.2

간호대학생의 무례 행동은 전문직관에 어긋날 뿐 아니라 미래에 간호 문화 및 환자 안전을 위협할 수 있으므로매우 심각하다. 따라서 본 연구는 텍스트네트워크분석을 적용하여 간호대학생 관련 무례 행동 연구의 동향을 살펴보았다. 연구과정은 문헌 수집, 단어 정제, 자료 분석 순이었다. 2015년 1월부터 2019년 10월까지 국제학술지에 출판된 관련논문 139편을 선정하여 논문 초록을 분석하였다. NetMiner 프로그램을 이용해 키워드 간 동시출현 행렬을 생성한후, 네트워크 특성 분석, 노드의 중심성 분석, 자아연결망 분석을 실시하였다. 연결중심성, 근접중심성, 매개중심성에서상위 30위 이내에 포함된 핵심주제어는 “간호학생(nursing student)”, “무례함(incivility)”, “간호학교수(nursing faculty)”, “지각(perception)”, “괴롭힘(bullying)”, “폭력(violence)”, “학습윤리(academic integrity)”, “공격(aggression)”, “사이버불링(cyberbullying)” 등이었다. 무례 행동에 해당하는 “무례함”, “괴롭힘”, “폭력”, “공격” 및 “학습윤리”에 대한 자아연결망분석을 실시한 결과, “무례함”의 에고넷 크기가 가장 컸고 이들 핵심주제어에 따라 관련 인물, 발생 장소 등에 차이가있었다. 본 연구를 통해 임상실습 현장에서 학생과 간호사, 보건의료인, 환자 사이에 발생하는 무례 행동 연구가활발함을 알 수 있었다. 학습윤리 역시 학교보다 임상실습 교육에서 더 자주 연구되고 있었는데 이에 대한 간호교육기관의 대응이 시급하다고 본다. 한편 무례 행동을 나타내는 용어가 연구자 간에 상이하게 사용되고 있으므로개념에 대한 합의가 선행되어야 하겠다.

미용서비스업 Direct Mail에 대한 인식과 만족도 및 구전의도 -남성을 대상으로-

박은준 한국미용학회 2009 한국미용학회 추계학술발표회 Vol.2009 No.1

연명의료 관련 신문 기사의 텍스트네트워크분석

박은준,안대웅,박찬숙 한국지역사회간호학회 2018 지역사회간호학회지 Vol.29 No.2

Purpose: This study tried to understand discourses of life-sustaining treatments in general daily and healthcare newspapers. Methods: A text-network analysis was conducted using the NetMiner program. Firstly, 572 articles from 11 daily newspapers and 258 articles from 8 healthcare newspapers were collected, which were published from August 2013 to October 2016. Secondly, keywords (semantic morphemes) were extracted from the articles and rearranged by removing stop-words, refining similar words, excluding non-relevant words, and defining meaningful phrases. Finally, co-occurrence matrices of the keywords with a frequency of 30 times or higher were developed and statistical measures―indices of degree and betweenness centrality, ego-networks, and clustering―were obtained. Results: In the general daily and healthcare newspapers, the top eight core keywords were common: “patients,” “death,” “LST (life-sustaining treatments),” “hospice palliative care,” “hospitals,” “family,” “opinion,” and “withdrawal.” There were also common subtopics shared by the general daily and healthcare newspapers: withdrawal of LST, hospice palliative care, National Bioethics Review Committee, and self-determination and proxy decision of patients and family. Additionally, the general daily newspapers included diverse social interest or events like well-dying, euthanasia, and the death of farmer Baek Nam-ki, whereas the healthcare newspapers discussed problems of the relevant laws, and insufficient infrastructure and low reimbursement for hospice-palliative care. Conclusion: The discourse that withdrawal of futile LST should be allowed according to the patient’s will was consistent in the newspapers. Given that newspaper articles influence knowledge and attitudes of the public, RNs are recommended to participate actively in public communication on LST.

고추의 소포자 배양 시 전처리 후 배지의 교환, 배지의 첨가 및 2층배양 시 하층고체 배지의 양이 배의 생산에 미치는 영향

박은준,이종숙,안동주,김문자 한국식물생명공학회 2010 식물생명공학회지 Vol.37 No.4

The effect of the addition of the fresh medium, volume of under solid medium in double layer culture as well as the medium change after pretreating microspores on the production of embryos in microspore culture of hot pepper (Capsicum annuum L.) has been studied. When cultured after heat pre-treatment, changing pretreatment media with fresh culture media proved to be more effective for embryo production rather than supplementing additional culture media. Heat-pretreating for 3 days turned out more effective for embryo production than pretreating for 1 or 2 days. In the case of anther pretreatment, the addition of fresh medium after culture was not effective for embryo production. In pretreating microspores, however, supplementing additional fresh culture media greatly improved embryo yield and quality. The best time point of media addition was 4 days after culture commenced, and the most effective number of times of media addition was one time addition. Moreover, the effective volume of added medium in double layer culture for embryo production was 1.5 ml. The addition of media more than 1.5 ml reduced both embryo yield and quality. Double layer medium was more effective for embryo development than liquid medium. When the volume of under solid medium increased ranging from 3 ml to 7 ml, more cotyledonary embryos were produced in either 5 ml or 7 ml compared to 3 ml, even though the total number of embryos were highest in 3 ml. These results can be used as an important data for establishing an efficient microspore culture system for producing high frequency of normal embryos in hot pepper. 본 연구에서는 고추의 소포자 배양 시 전처리 후 배지의 교환 여부, 배양 후 새 배지의 첨가 및 2층배지 사용시 하층고체배지의 양이 배의 생산에 미치는 영향을조사하였다. 고온 전처리 후 배양배지를 첨가하여 배양하는 것보다 사용한 전처리 배지를 새 배양 배지로 교환하여 배양 하는 것이 배의 생산에 효과적 이었으며,고온전처리 기간은 1일이나 2일에 비해 3일이 효과적 이었다. 배양 후 새 배지의 첨가는 약전처리 시에는 효과가 없었으나 소포자 전처리 시에는 배의 유기와 발달모두 크게 향상되었다. 새 배지의 첨가 시기는 배양 4일후가, 첨가 횟수는 1회가 배의 생산에 가장 효과적이었다. 한편 2층배지 사용 시 첨가하는 새 배지의 양은 1.5 ml이효과적이었으며 이보다 많은 양을 첨가하는 경우 배의발생과 발달 모두 저하되었다. 액체배지 사용 시에 비해 2층배지 사용 시 배의 발달이 좋았다. 또 2층배지 사용 시 하층고체배지의 양이 3 ml 일 때 보다는 5 ml이나 7 ml일 때 배의 발생은 감소하였으나 배의 질이 향상되었다. 이와 같은 결과들은 고추에서 다수의 정상자엽배를 생산 할 수 있는 소포자 배양시스템을 확립하는데 중요한 기초자료가 될 것이다.

고추 소포자를 이용한 반수체 및 배가반수체 생산

박은준,안율균,권덕호,양은영,Eun Joon Park,Yul Kyun Ahn,Doek Ho Kwon,Eun Young Yang Korea National University of Agriculture and Fishe 2024 현장농업연구지 = Journal of practical agricultural resear Vol.25 No.4

Haploid/double haploid plants developed from isolated microspores can significantly accelerate plant breeding. Haploid plants can naturally double their chromosomes to create a pure homozygous line of diploid plants. We present a method for producing embryos from isolated microspores of hot peppers (Capsicum annuumL.). We analyzed the polyploidization levels of the regenerated plants. The donor plants produced the optimal stage of microspores following short-term growth under low-intensity light, which resulted in high rates of embryogenesis and cotyledonary embryogenesis. To find an efficient culture method, liquid, doubled-layer, and 2-step cultures were tested. Liquid culture yielded the highest number of embryos, whereas the highest efficiency for cotyledonary embryogenesis was afforded by the doubled-layer culture. When normal cotyledonary embryos were transplanted onto a regeneration medium, they developed into complete plants. From these, 208 plants were tested via flow cytometric analysis, and 35.6% and 72.7% of the chromosomes from the Milyang-jare and LV2319 genotypes, respectively, were found to be spontaneous double haploids. These results are the same as those obtained on analyzing horticultural characteristics, including the size of leaves and the size and shape of fruits. The present study provides information on the practical application of isolated microspore culture of hot peppers, factors that affect embryogenesis, and methods for polyploidy testing.

간호교육 현장의 무례함

박은준 한국간호윤리학회 2018 한국간호윤리학회 학술대회 Vol.2018 No.11

고추의 소포자 배양 시 전처리 배지, 새 배지의 첨가, 및 배양 용기의 크기가 배의 생산에 미치는 영향

박은준,김진애,김문자 한국식물생명공학회 2009 식물생명공학회지 Vol.36 No.2

The influences of pretreatment medium, the addition of fresh medium, and the size of culture plate on the production of embryos were investigated in isolated microspore culture of hot pepper (Capsicum annuum L.). Among the media used for heat shock pretreatment (32±1℃), high frequency embryo production was obtained when the sucrose- starvation medium A was used. On the other hand, neither 0.37 M mannitol solution nor NLNS medium supplemented with sucrose was not efficient for embryo production. The addition of culture medium to pretreatment media considerably decreased the embryo production even though embryo development proceeded further. The embryo production was not improved by the addition of fresh medium after 2 or 3 weeks from starting culture. Increase in the size of the culture plate from 3.5×1.0 cm to 6.0×1.5 cm improved embryo quality. These results will provide valuable information for developing an efficient microspore culture system of hot pepper for high frequency embryo production. 고추의 소포자 배양 시 전처리 배지, 배양 후 새 배지의 첨가, 및 배양 용기의 크기가 배의 발생 및 발달에 미치는 영향을 조사하 였다. 고온 전처리 (32±1℃) 시 사용된 배지 중 배의 생산에 적합한 것은 당-기아배지 A이었으며, 0.37 M mannitol 용액이나 sucrose가 첨가된 NLNS배지는 배의 생산에 비효율적이었다. 전처리 배지인 당-기아배지 A와 0.37 M mannitol 용액에 배양배지를 첨가하는 경 우 배의 발달 정도는 증가하였으나 배의 생산은 크게 감소하였다. 배양 2, 3 주 후의 새 배지 첨가는 배의 생산에 효과가 없었다. 배 양용기는 3.5×1.0 cm 크기의 것 보다는 6.0×1.5 cm 크기의 것을 사 용하는 것이 배의 발달에 효과적이었다. 이상에서와 같은 결과들 은 고추에서 다수의 배를 생산할 수 있는 소포자 배양시스템을 확 립하는데 중요한 기초자료가 될 것이다.

Usability and Accessibility Evaluation of South Korean and American Health-Related Websites for the Elderly

박은준,고지운 한국간호과학회 간호행정학회 2010 간호행정학회지 Vol.16 No.4