Expression of Glycolate Oxidase Gene in Spinach

박양서,최정도,조남정,Park, Yang-Seo,Choi, Jung-Do,Cho, Nam-Jeong 생화학분자생물학회 1992 한국생화학회지 Vol.25 No.3

시금치 glycolate oxidase 유전자의 성질을 Southern 그리고 Northern blot hybridization 방법으로 조사하였다. EcoRI 또는 HindIII 제한효소로 처리한 genomic DNA를 시금치 glycolate oxidase cDNA를 probe로 사용하여 Southern blot 방법으로 분석한 결과 여러 개의 band가 관찰되었다. 이 효소 유전자의 발현양상은 Northern blot 방법으로 분석하였다. 이 유전자의 발현활성은 파종 후 8-35일 동안 비슷하였다. Glycolate oxidase mRNA는 잎에서는 많이, 그리고 줄기에서는 약간 발견되었으나, 뿌리에서는 거의 발견되지 않았는데, 이 결과는 이 효소 유전자의 발현이 조직에 따라 다름을 나타낸다. Glycolate oxidase mRNA의 양은 식물을 암 소에서 배양하였을 때는 상당히 감소하였으나, 빛을 쪼여주자 그 양이 증가하였다. 이련 결과는 빛이 glycolate oxidase 유전자의 발현올 촉진한다는 것을 보여준다. The nature of glycolate oxidase gene in spinach was investigated by Southern and Northern blot hybridization. Genomic DNA digested with EcoRI or HindIII exhibited multiple bands when hybridized with a spinach glycolate oxidase cDNA. Expression patterns of the gene were analyzed by Northern blot hybridization. The activity of the gene expression was found to be similar during 8~35 days of cultivation. The level of the glycolate oxidase mRNA was high in leaves, low in stems and little in roots, indicating that the expression of the gene is tissue-dependent. The mRNA level was considerably reduced when the plants were grown in dark. However, the level was increased when the dark-grown plants were subjected to illumination. These results show that light serves as a signal for the activation of the glycolate oxidase gene expression.

시금치 glycolate oxidase 유전자의 발현

박양서,최정도,조남정 ( Yang Seo Park,Jung Do Choi,Nam Jeong Cho ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.3

The nature of glycolate oxidase gene in spinach was investigated by Southern and Northern blot hybridization. Genomic DNA digested with EcoRI or HindIII exhibited multiple bands when hybridized with a spinach glycolate oxidase cDNA_ Expression patterns of the gene were analyzed by Northern blot hybridization. The activity of the gene expression was found to be similar during 8∼35 days of cultivation. The level of the glycolate oxidase mRNA was high in leaves, low in stems and little in roots, indicating that the expression of the gene is tissue-dependent. The mRNA level was considerably reduced when the plants were grown in dark. However, the level was increased when the dark-grown plants were subjected to illumination. These results show that light serves as a signal for the activation of the glycolate oxidase gene expression.

시금치 Glycolate Oxidase의 활성부위에 관한 연구

손의동,박양서,조태주,최정도,조남정,Son, Eui-Dong,Park, Yang-Seo,Cho, Tae-Ju,Choi, Jung-Do,Cho, Nam-Jeong 생화학분자생물학회 1994 한국생화학회지 Vol.27 No.5

Glycolate oxidase는 식물의 광호흡과정에 관여하는 효소 중 하나로서 glycolate를 glyoxylate로 산화시키는 반응을 매개한다. 시금치 glycolate oxidase에 대한 X-ray crystallography 연구에 의하면, histidine 254와 arginine 257이 효소작용에 중요한 역할을 수행하리라 추정된다. 이런 가설을 검증하기 위하여 본 연구에서는 site-directed mutagenesis 방법을 사용하여 이 효소의 histidine 254을 asparagine 또는 tyrosine으로, arginine 257을 serine으로 치환하였다. Wild type에서는 0.125mM의 기질에서도 강한 효소활성이 나타난 반면, mutant들의 경우에는 40mM 기질의 존재하에서도 효소활성이 거의 관측되지 않았다. 본 연구의 실험결과는 histidine 254와 arginine 257이 시금치 glycolate oxidase의 작용기작에 필수적인 아미노산이라는 것을 시사해 준다. Glycolate oxidase is one of the key enzymes in photo respiration, where it oxidizes glycolate to glyoxylate. Previous X-ray crystallographic studies on spinach glycolate oxidase suggested that histidine 254 and arginine 257 are important in the enzyme function. To evaluate this postulation, we replaced histidine 254 with asparagine or tyrosine and arginine 257 with serine by means of site-directed mutagenesis. The mutants xhibited little, if any, enzymatic activity with 40 mM glycolate, whereas the wild type showed a strong enzymatic activity in the presence of 0.125 mM glycolate. These results imply that histidine 254 and arginine 257 play essential roles in the action of spinach glycolate oxidase.

시금치 Glycolate Oxidase 의 활성부위에 관한 연구

손의동,박양서,조태주,최정도,조남정 ( Eui Dong Son,Yang Seo Park,Tae Ju Cho,Jung Do Choi,Nam Jeong Cho ) 생화학분자생물학회 1994 BMB Reports Vol.27 No.5

Glycolate oxidase is one of the key enzymes in photorespimtion, where it oxidizes glycolate to glyoxylate. Previous X-ray crystallographic studies on spinach glycolate oxidase suggested that histidine 254 and arginine 257 are important in the enzyme function. To evaluate this postulation, we replaced histidine 254 with aspamgine or tyrosine and arginine 257 with serine by means of site-directed mutagenesis. The mutants exhibited little, if any, enzymatic activity with 40 mM glycolate, whereas the wild type showed a strong enzymatic activity in the presence of 0.125 mM glycolate. These results imply that histidine 254 and arginine 257 play essential roles in the action of spinach glycolate oxidase.

원발성 난소 이행세포암

박영순 ( Young Soon Park ),이상호 ( Sang Ho Lee ),정영환 ( Young Hwan Chung ),고준성 ( Sung Ko ),문종수 ( Zong Soo Moon ),서수형 ( Soo Hyung Seo ),박양서 ( Yang Suh Park ) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.9

Primary ovarian transitional cell carcinoma (TCC) is extremly rare tumor and has been recently classified into the one of the histologic subtypes of ovarian epithelial cell carcinomas. Primary ovarian TCC has been proven to differ from malignant Brenner tumor (MBT) and TCC of th bladder for it`s histologic characteristics as well as therapeutic and prognostic implications. TCC of the ovary has been also recognized to have excellent response to chemotherapy and favorable prognosis although it`s aggressive properties. We experienced a case of primary ovarian TCC (stage; Ⅱc) in 57 year old woman who presented with low abdominal pain and report this case with a brief review of the concerned literatures.

SEMM 식 자궁적출술에 대한 임상적 고찰

우소영(So Young woo),박영순(Young Soon Park),이상호(Sang Ho Lee),문종수(Zong Soo Moon),서수형(Soo Hyung Seo),박양서(Yang Suh Park) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.10

N/A Objectives : We performed this study to review the clinical availability of the classical intrafascial SEMM hysterectomy (CISH), and report our experience for 2 years restrospectively. Methods : From March 1999 to February 2001, 100 cases of CISH were performed at the Department of Obstetrics and Gynecology, College of Medicine, Kangdong Sacred Heart Hospital, Hallym university. We performed this procedure by 3 steps. The dissection of uterus was done by classical suture methods (including extracorporeal and intracorporeal suture techniques) to uterine isthmic portion. And then transvaginal cylindrical coring out of the cervical tissue was done using a cervix corer, the Calibrated Uterine Resection Tools (CURT) for resection of cervical tissue especially transformation zone. Finally, the dissected uterus was extracted by using a Serrated Edged Macro-Morcellator (SEMM) without colpotomy. We reviewed the patient's data retrospectively and made phone calls to each patients. Results : The indications for CISH, based on the pre-and intraoperative diagnosis, were leiomyoma, adenomyosis, endometrial hyperplasia and chronic pelvic inflammatory disease or pelvic pain, in order of frequency. The mean operative time was 126 minutes and the mean hemoglobin change was 1.27 gm/dL. The mean hospital days were 5.7 days and the mean age of the patients was 44.6 years old. There were no definite intra- and postoperative complications except for 3 cases of CO2 retentions and 2 cases of delayed bleeding on cervical stump. The mean days to first sexual contact after surgery were 34.5 days. In view of sexual feeling, 86% of the patients didn't feel any difference and only 13% felt worse than before. During postoperative follow-up, the cervical cytologic study were found no endocervical cells at all of 44 checked patients. Conclusion : The CISH procedures can be safely performed by well-trained laparoscopists, and this technique minimally alters the anatomy and integrity of the pelvic floor and vagina. Therefore the reductions of surgical morbidity, blood loss, hospitalization and postoperatve discomfort were obtained. Through the preservation of the anatomical relations of the pelvic floor and the function of female sexuality, this technique is the new proposal in gynecologic surgery.

임신중기 양수천자의 임상적 고찰

이상호(Sang Ho Lee),박영순(Young Soon Park),장재혁(Jae Hyuk Chang),문종수(Zong Soo Moon),서수형(Soo Hyung Seo),박양서(Yang Suh Park),윤숙경(Sook Kyung Yun) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.7

목적 : 본 연구는 세포유전학 검사를 위한 임신중기 양수천자의 적응증과 염색체 핵형결과를 평가하고 이에 따른 염색체이상의 발생빈도와 상관관계를 분석하고 양수천자의 안정성 및 합병증을 평가하기 위하여 시행하였다. 연구 방법 : 본 연구는 1997년 1월부터 2001년 10월까지 한림대학교 강동성심병원 산부인과에서 세포유전학 검사의 적응증에 해당되어 양수천자를 시행받은 674명의 임신부를 대상으로 대상환자의 연령과 임신주수의 분포, 양수천자의 적응증과 염색체 핵형검사의 결과 그리고 각 적응증에 따른 염색체이상의 결과와 양수천자술의 안정성 및 합병증을 평가하였다. 결과 : 양수천자의 적응증은 산모 혈청표지자 선별검사의 이상이 46.2%로 가장 많았으며 고령산모 (≥35)가 44.8%, 초음파에서 이상소견을 보인 경우가 5.9%, 태아기형아의 가계력을 가진 경우가 2.8%, 염색체이상아의 출산력을 가진 경우가 0.3% 순이었다. 연령별로는 35~39세의 연령군이 34.6%로 가장 많았으며, 주수별로는 15∼19주 사이가 69.9%으로 가장 많았고 조기 양수천자에 해당하는 14주 이전에 실시한 경우가 1.0%였다. 염색체 핵형분석의 결과 비정상핵형은 3.6% (24/674)였으며, 이 중 수적이상이 18예, 구조적 이상이 6예였다. 수적이상에는 상염색체이상으로 다운증후군 7예 (전좌형 1예), 에드워드증후군 6예, 파타우증후군 1예와 그외 3예의 모자이시즘이 있었고, 성염색체이상으로 터너증후군이 1예 있었다. 적응증별 염색체이상 비율은 산모 혈청표지자 선별검사 이상에서 3.2% (10/311), 고령산모 중에서는 3.0% (9/302)로 유의한 차이를 보이지는 않았으나 초음파에서 이상소견을 보인 경우가 7.5% (3/40)으로 유의한 차이를 보였다. 염색체이상아의 출산력을 가진 경우는 2예 모두에서 비정상 핵형을 보였지만 그 대상 환자군이 적어 평가를 할 수 없었다. 결론 : 고령산모나 산모 혈청표지자 선별검사 그리고 초음파검사 등을 통하여 유전질환의 위험성이 높은 산모를 선별하고, 이들 산모를 대상으로 임신중기 양수천자를 통한 태아의 세포유전학적 검사는 산전 유전질환의 진단법으로 매우 유용한 검사이다. 또 이러한 세포유전학적 연구결과는 산전진찰 및 유전학적 상담의 중요한 자료로 활용될 수 있을 것이며 이에 대한 지속적인 연구와 보고가 더욱 필요하리라 사료된다. Objective : We performed this study to analyze the indications, distributions of maternal age and cytogenetic results of midtrimester amniocentesis and to determine the complications and risks of it. Methods : This study was retrospectively reviewed 674 cases of midtrimester genetic amniocentesis from January 1997 to October 2001 in Kangdong Sacred Hospital, Hallym University. We analyzed the indications, distributions of maternal and gestational age, chromosomal results, and of which accorded to indications of cytogenetic studies. Results : The indications of amniocentesis were abnormal maternal serum screening (46.2%), advanced maternal age (≥35: 44.8%), abnormal ultrasonographic findings (5.9%), familial history of fetal anomaly (2.8%) and previous history of fetal chromosomal abnormalities (0.3%), in order. The overall incidence of chromosomal aberration was 3.6% (24/674), which were composed of 18 cases of numerical aberrations and 6 cases of structural aberrations. In autosomal aberrations, there were 7 cases of Down`s syndrome, 6 cases of Edward syndrome, 1 case of Patau syndrome and 3 cases of Mosaicism. In sex chromosomal aberration, there was a case of Turner syndrome. In chromosomal aberrations according to indications, there was 7.5% (3/40) of chromosomal abnormalities in abnormal ultrasonographic findings group, 3.2% (10/311) in abnormal maternal serum screening group and 3.0% (9/302) in advanced maternal age group. There was statistical significance only in abnormal ultrasonographic findings group. There was no significant complication after amniocentesis except 0.3% (2 cases) of fetal loss. Conclusion : Maternal serum screening, advanced maternal age and antenatal ultrasonograms should be important screening methods for amniocentesis which is considered to the most effective diagnostic procedure for prenatal cytogenetic studies. The karyotyping analysis of midtrimester amniocentesis is efficacious method for evaluation of chromosomal aberrations and genetic counselling for parents.

복막에 발생한 원발성 유두양 장액성 악성종양

권민연(Min Yeon Kwon),이존익(John Ik Lee),우소영(So Young Woo),최규하(Kyu Ha Choi),박주진(Choo Jin Park),김덕환(Duck Hwan Kim),문종수(Zong Soo Moon),서수형(Soo Hyung Seo),박양서(Yang Suh Park) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.8

Primary papillary serous carcinoma of the peritoneum(PPSCP) is vere rare. It has been suggested that PPSCP derives from embryonal coelomic epithelium with m llerian ducts potential. PPSCP can develop from a single or multicentric focus. The clinical and histologic disease entities are similar to those of primary papillary serous carcinoma of the ovary, but PPSCP involves the ovarian surface only minimally(microscopic disease) or spares the ovaries entirely. We have experienced a case of primary papillary serous carcinoma of the peritoneum and report this case with brief review of the concerned literature.

자연생리주기에서 발생한 병합임신 1례

송성욱 ( Sung Wook Song ),정영환 ( Young Whan Jung ),문종수 ( Zong Soo Moon ),서수형 ( Soo Hyung Seo ),박양서 ( Yang Suk Park ) 대한주산의학회 2001 Perinatology Vol.12 No.2

임신 중 발생한 결핵성 복막염

이존익(John Ik Lee),권민연(Min Yeon Kwon),송성욱(Sung Wook Song),박주진(Choo Jin Park),문종수(Zong Soo Moon),서수형(Soo Hyung Seo),박양서(Yang Suh Park) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.1

The tuberculous peritonitis, especially in pregnancy, is very rare and difficult to diagnose since there is no diagnostic clue and limitation of diagnostic means. The clinical features may vary such as fever, chill, tachycardia, abdominal tenderness or rebound tenderness which are not controlled easily with antibiotics. It is possible to diagnose by AFB culture and biopsy through exploro-laparotomy which is done due to uncontrolled symptoms even to unstable vital signs. It's clinical symptoms and signs are dramatically improved with antituberculotic therapy. Recently we have experienced a case of tubeculous peritonitis at 29 weeks gestation which was diagnosed through exploro-laparotomy including cesarean section. So we report this case with a brief review of literature