ESSENTIAL ARGINYL RESIDUE ( S ) OF GLYCOLATE OXIDASE FROM RAT LIVER

최정도 한국생화학분자생물학회 (구 한국생화학회) 1987 추계학술대회집 Vol.- No.-

정보화 입문자의 첫발 떼기를 위한 디딤돌 - 고광윤(2019)를 중심으로 -

최정도 연세대학교 언어정보연구원 2022 언어사실과 관점 Vol.55 No.-

The purpose of this research is to review The ANTCONC Book, a book that helpscorpus researchers use corpus tools. In Korea, the guidebook of corpus tools isquite rare. It is because there are not many corpus search tool available for free. It is also a problem with the perception of the necessity of using corpus. As aresult, it is still a difficult problem for Korean researchers to use corpus. Thisbook explains in great detail AntConc, a tool for using corpus that can be usedfor free. That's why it's very helpful to people who are new to Koreaninformatization. In addition, this book takes a method of explaining in greatdetail, and the latter part is designed to make good use of AntConc by presentingpractice questions. Although English corpus-centered explanations are dominant,it is thought to be of considerable help in the use of Korean corpus. After that,it is expected that tools and applications that can use Korean corpus will besteadily developed. .

‘-ㄴ지’의 문법적 지위에 대한 비판적 고찰 -내포문에서의 쓰임을 중심으로-

최정도,김문기 우리말학회 2013 우리말연구 Vol.33 No.-

이 글은 그간 종결 어미, 연결 어미, 명사형 어미 등으로 논의되 어 온 내포문에서 쓰이는 ‘-ㄴ지’의 범주에 대해서 비판적으로 고찰한 것 이다. 먼저 2장에서 ‘-ㄴ지’의 범주에 대해 살펴본 앞선 연구들을 개관하고 ‘-ㄴ지’의 범주를 달리 규정하고 있는 쟁점을 정리하였다. 그리고 3장에서 는 사전에서 ‘-ㄴ지’를 어떻게 기술하고 있는지를 살펴보면서 그 양상을 비판적으로 검토하였다. 4장에서는 2장과 3장에서 검토한 것을 바탕으로 ‘-ㄴ지’의 범주에 대한 논의를 비판적으로 검토하였다. 이때 중요한 것은 공시적인 사실에 바탕을 두면서 통시적인 사실을 참고해야 한다는 것이 다. 그 결과 ‘-ㄴ지’는 역사적인 발달 과정에서 그 구성이 굳어져 현대에까 지 이르고 있으므로 어말 어미임에는 분명하나, 어말 어미 체계 내에서 그 범주가 무엇이라 판단 내리기 힘든 상태에 있다는 것을 확인하였다.

FAD-independent and Herbicide-resistant Mutants of Tobacco Acetohydroxy Acid Synthase

최정도,Dung Tien Lea 대한화학회 2005 Bulletin of the Korean Chemical Society Vol.26 No.6

Acetohydroxy acid synthase catalyzes the first common step in the biosynthesis of branched chain amino acids. AHAS plays two distinct metabolic roles, and is designated as anabolic AHAS and catabolic AHAS, depending on its function. Anabolic AHAS is FAD-dependent, while its catabolic counterpart is not. In this work, a conserved motif was identified in the -domain of anabolic AHASs, but not in catabolic AHAS (372RFDDR376). In order to determine the functions of this motif, we replaced the motif with the corresponding sequence in FAD-independent AHAS, SPVEY. None of these three mutants (SPV, SPVE, and SPVEY) was detected with bound FAD. However, two of these mutants (SPVE and SPVEY) were active at a low level of specific activity. Although they exhibited pyruvate- and ThDP- dependent characteristics, the activity of the two active mutants appears to be FAD-independent. The SPVEY mutant was completely insensitive to the three tested herbicides, even at extremely high concentrations and is also somewhat more thermolabile than the wild type enzyme. The data provided in this work suggest that the RFDDR motif is a possible determinant of the FAD-dependent and herbicide-resistant properties of tobacco AHAS. The SPVEY mutant appears to exhibit catabolic AHAS-like activity.

“국회 회의록 말뭉치 구축에 관하여”에 대한 토론문

최정도 한국사전학회 2022 한국사전학회 학술대회 발표논문집 Vol.2022 No.2

말뭉치를 이용한 사전 편찬에서의 몇 문제에 대하여

최정도 한국사전학회 2010 한국사전학회 학술대회 발표논문집 Vol.2010 No.2

Glycolate Oxidase 에 관한 흡광 및 형광의 분광학적 연구

최정도,박금수,양덕조 ( Jung Do Choi,Kum Soo Park,Duk Jo Yang ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.3

Absorption and fluorescence spectroscopic studies of glycolate oxidase were carried out to elucidate the properties of the active site of the enzyme. The electronic spectrum of FMN bound to the enzyme consists of two bands centered around 450 nm and 340 nm in the region of 300-500 nm. Comparing with the spectra of model systems, it is suggested that the bound FMN existes almost as non-hydrogen-bounded species, and the environment of the FMN is much less polar than water medium. The fluorescence of the bound FMN is only about 5% as great as that of free FMN indicating strong interactions between flavin moiety and aromatic amino acid residues of the protein. The fluorescence quenching data of free FMN and bound FMN suggest that the coenzyme FMN is not exposed on the surface and most likely located in a crevice whose dimensions are not large enough for large hydrated ions to enter it. The cysteine residue, a subunit of the enzyme is known to have one cysteine, is accesible to silver ion and probably located not at active site but near active site.

쥐 간의 Glycolate Oxidase 의 필수 Arginine

최정도,Kihachiro Horiike,Mitsuhiro Nozaki ( Jung Do Choi,Kihachiro horiike . Mitsuhiro Nozaki ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.3

Glycolate oxidase from rat liver is inactivated by arginine-specific reagents, phenylglyoxal and 2,3-butanedione. Inactivation of the oxidase by phenylglyoxal follows pseudo-first order kinetics, and is first order with respect to the reagent concentration. The oxidase is completely inactivated by prolonged incubation with phenylglyoxal. The substrate glycolate and substrate-competitive inhibitors, phenyllactic acid and oxalate protect the oxidase against inactivation. Other dicarbonyl compound butanedione also reacts with the enzyme to cause loss of activity but at much slower rate. Inactivation by butanedione is also dependent on the reagent concentration, and enhanced by the presence of borate buffer. Kinetic studies with the enzyme partially inactivated by phenylglyoxal or butanedione demonstrate that Km value of glycolate is similar to the constant for the control enzyme. Although amino acid analysis data do not provide the precise number of arginine modified, a few arginyl residues out of the 15 total residues in the subunit seem to be modified upon 80% inactivation by phenylglyoxal, and competitive inhibitor provides some protection against modification of arginyl residues. The present data suggest that arginyl residue(s) is probably involved in the binding of substrate to the enzyme.

한국어 교육용 접사 선정 방법론 연구

최정도 명지대학교(서울캠퍼스) 인문과학연구소 2021 인문과학연구논총 Vol.42 No.1

The purpose of this study is to propose a new affix(preffix, suffix) selection methodology for derivative training. It is a preliminary discussion for selecting the affixes of derivative training for vocabulary expansion of Korean learners. In this discussion, 5 million corpus callsfor five resisters were used to analyze all 530 affixes. Then, the frequency of the affix was calculated, the number of derivative types produced by the affix, and the pattern of use by resister of use of the affix were presented. As a result, it was argued that the frequency of use of affixes, the number of derivative types, and that the affixes used in various resister should be selected as educational affixes. As a result, based on the analysis results of corpus, such as those established in this discussion, it is expected that each teacher or textbook writer will be able to select and use the important affixes for vocabulary education. 이 연구는 파생어 교육을 위한 새로운 접사 선정 방법론을 제안하는 것으로한국어 학습자의 어휘 확장을 위한 파생어 교육의 접사 선정을 위한 예비적 논의이다. 그간의 연구는 교재에서 제시된 파생어를 분석하거나 기구축 어휘 목록등을 이용한 파생 접사 선정을 통해서 이를 통한 한국어 단어 형성법 교육이필요하다는 주장들이 이어져 왔다. 하지만 한국어 단어 형성의 양상을 제대로포착하여 학습자들에게 실제 도움을 줄 수 있는 접사 선정은 접사의 사용 양상을 확인하기 위한 기초 자료 구축에서부터 고심할 필요가 있다. 이 논의에서는5개 영역의 500만 말뭉치를 이용하여 표준국어대사전에 접사로 등재된 530여개의 접사를 모두 분석하는 방식을 취하였다. 그런 다음 접사 빈도 산출, 해당접사로 생산된 파생어 유형 수, 접사의 사용역별 사용 양상 등을 제시하였다. 그 결과 접사의 사용 빈도가 높고, 파생어 유형 수도 많으며, 다양한 사용역에서 사용되는 접사를 교육용 접사로 선정할 것을 주장하였다. 최종적으로 이 논의에서 구축한 것과 같은 기초 자료의 분석 결과를 토대로, 교수자나 교재 집필자들마다 중요하다고 생각하는 접사를 선정하여 한국어 어휘 교육에 활용할 수있을 것이다.

해양스포츠시설 구성원의 임파워먼트에 관한 연구

최정도 한국스포츠리서치 2005 한국 스포츠 리서치 Vol.16 No.3