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Disorder of Sexual Development in a Cocker Spaniel Dog: Sry-negative XX Sex Reversal
이성림,맹근호,김나현,박기태,이희천,이재훈 한국임상수의학회 2012 한국임상수의학회지 Vol.29 No.5
A 3-year-old Cocker spaniel was presented for evaluation because of a mass protruding from the vulva. This mass confirmed the presence of an internal bone in clitoris on the radiography. We recommended to the owner of the surgical removal of the gonads and uterus as well as the excision of the enlarged clitoris and os clitoris. The dog had gonads in the region of where the ovaries were located and presented what appeared to be a bicornate uterus and ductus deferens. Histological analysis revealed a morphologically normal uterus and two testes with their epididymis and ductus deferens. Karyotyping revealed a normal female chromosome constitution (78, XX), and polymerase chain reaction (PCR) identified the absence of Sry. Based on these findings, the patient was diagnosed with Sry-negative XX sex reversal.
Anti-Prolactin과 다양한 호르몬 투여에 따른 개의 발정 유도 효율 비교
허영,강은주,맹근호,김민정,조규완,이성림 韓國受精卵移植學會 2009 한국동물생명공학회지 Vol.24 No.3
Domestic bitches are non-seasonally monoestrus; spontaneously ovulate only once or twice occurs at anytime of the year. Estrus induction has been applied infrequent estrus, misleading ovulation, mating difficulties, failure to conceive after normal mating, pregnancy failure and biological research. Protocol of estrus induction which included variable hormones such as FSH, GnRH, and PMSG have been applied for the last decades. Recently, Bromocriptine, one of anti-prolatin/dopamine agonist has been occasionally applied for estrus induction. The study was carried out to investigate the effective method for the induction of estrus in bitches using different hormone treatments, and the initiation time of estrus from hormone treatment by assessments of cytological observation and blood plasma progesterone concentration. A total of 54 bitches on anestrus were selected for the study and divided randomly into 8 treatment groups as follow. Control, natural estrus; FSH (L), FSH (1.5 mg/kg, twice a day, , Vetrepharm); FSH (H), FSH (3.0 mg/kg, twice a day); GnRH+FSH, GnRH (5 ug/kg, once first day, , Dongbang)+FSH (3 mg/kg, SID); PMSG, PMSG (50 IU/kg, every third day , Intevet); GnRH+PMSG, GnRH (5 ug/kg, only first day)+PMSG (50 IU/kg, every third day); GnRH, GnRH (5 ug/kg, only first day); Bromocriptine, bromocriptine (0.3 mg/kg, SID, , Novartis). The bitches were evaluated clinical sign, cytological exam and Premate for assessment of estrus induction. Estrus induction rates were significantly (P<0.05) higher in GnRH+PMSG (100%) compared to others. PMSG and GnRH+PMGS (87.5 and 100%) and Bromocriptine (77.8%) were higher than others except GnRH+PMSG. Analysis of vaginal smear has proved to be effective a correct assessment of estrus induction with assay of progesterone concentration by Premate. Proestrus initiated by the after induction in most case. In conclusion, bromocriptine is an effective drug for estrus induction in bitches and assay of progesterone concentration by Premate with examination of vaginal smear that should be useful to detection of estrus induction of estrus induced bitches.
LED 디스플레이 숙련도시험 시료의 안정성 판정 방법 연구
남효재(Hyo-Jae Nam),맹근호(Geun-Ho Maeng),정영호(Yeong-Ho Jeong) 한국조명·전기설비학회 2011 한국조명·전기설비학회 학술대회논문집 Vol.2011 No.11
The confidence of measurement results is important in native and foreign. Although the measurement uncertainty is used for confidence of measurement results, but it is difficult to confirm that there are the errors in the measurement methods and bias in the measurement results continuously. It is difficult to solve these problems, because there is no proficiency testing in LED display field. The coordinator of proficiency testing should consider homogeneity, stability, assigned value, standard deviation and performance statistics to realize the purpose of proficiency testing. The regression analysis is suggested for stability evaluation of artifact in this thesis. The relations between time and measurement results could be identified by regression analysis.
미니돼지에서 자가 피부유래 간엽성 줄기세포를 이용한 상악동저 거상술
변준호(June-Ho Byun),강은주(Eun-Ju Kang),맹근호(Geun-Ho Maeng),노규진(Gyu-Jin Rho),강동호(Dong-Ho Kang),이종실(Jong-Sil Lee),박봉욱(Bong-Wook Park) 대한구강악안면외과학회 2010 대한구강악안면외과학회지 Vol.36 No.2
Introduction: In our previous studies, we isolated porcine skin-derived mesenchymal stem cells (pSDMSCs) from the ears of adult miniature pigs and evaluated the pluripotency of these pSDMSCs based on expressions of transcription factors, such as Oct-4, Sox-2, and Nanog. Moreover, the characteristic of mesenchymal stem cells was revealed by the expression of various mesenchymal stem cell markers, including CD29, CD44, CD90, and vimentin. The aim of this study was to evaluate in vivo osteogenesis after maxillary sinus lift procedures with autogenous pSDMSCs and scaffold. Materials and Methods: The autogenous pSDMSCs were isolated from the 4 miniature pigs, and cultured to 3rd passage with same methods of our previous studies. After cell membranes were labeled using a PKH26, 1×107 cells/100 μL of autogenous pSDMSCs were grafted into the maxillary sinus with a demineralized bone matrix (DBM) and fibrin glue scaffold. In the contralateral control side, only a scaffold was grafted, without SDMSCs. After two animals each were euthanized at 2 and 4 weeks after grafting, the in vivo osteogenesis was evaluated with histolomorphometric and osteocalcin immunohistochemical studies. Results: In vivo PKH26 expression was detected in all specimens at 2 and 4 weeks after grafting. Trabecular bone formation and osteocalcin expression were more pronounced around the grafted materials in the autogenous pSDMSCs-grafted group compared to the control group. Newly generated bone was observed growing from the periphery to the center of the grafted material. Conclusion: The results of the present study suggest that autogenous skin-derived mesenchymal stem cells grafting with a DBM and fibrin glue scaffold can be a predictable method in the maxillary sinus floor elevation technique for implant surgery.