Quercetin Suppresses MIP-1a–Induced Adipose Inflammation by Downregulating Its Receptors CCR1/CCR5 and Inhibiting Inflammatory Signaling

노혜지,김추숙,강지혜,박준영,최석영,홍순명,유훈,박태선,유리나 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.5

Obesity-induced inflammation is characterized by recruitment of adipose tissue macrophages that release inflammatory cytokines and chemokines. MIP-1a (macrophage inflammatory protein 1a)/CCL3, a CC chemokine, induces monocyte/macrophage infiltration and thus is implicated in obesity-induced adipose inflammation. Quercetin has been shown to modulate obesity-induced inflammation, but the mechanism of its action remains unclear. Here we demonstrate that quercetin decreases MIP-1a release from adipocytes and macrophages and from cocultured adipocytes/macrophages; it also opposes MIP-1a–induced macrophage infiltration and activation. The inhibitory action of quercetin on the MIP-1a–induced inflammatory responses of macrophages is mediated by downregulation of CCR1/CCR5, and inhibition of activation of JNK, p38 mitogen-activated-protein kinase (MAPK), and IKK as well as IjBa degradation. These findings suggest that quercetin may be a useful agent against obesity-induced adipose tissue inflammation.

킬레이트 유기 미네랄 액비가 '죽향' 딸기의 생육 및 과실품질에 미치는 영향

노혜지,채효석,조향현,이철규,Noh, Hye-Ji,Chae, Hyo-Seok,Cho, Hyang-Hyun,Lee, Cheol-Gyu 한국유기농업학회 2017 韓國有機農業學會誌 Vol.25 No.4

본 연구는 수경재배시스템에서 킬레이트 유기 미네랄 액상비료인 AGH-Ringer의 관주가 '죽향' 딸기의 생육과 과실 수량 및 품질에 미치는 영향을 조사하기 위하여 수행하였다. 그 결과 AGH-Ringer는 대조군에 비해 초장의 증가와 함께 엽장과 엽폭이 각각 0.47 cm, 0.7 cm 증가함에 따라 엽면적이 유의적으로 22.2% 증가하였다. 또한, AGH-Ringer에 의해 잎의 생체중과 건물중 및 엽색도를 측정하는 SPAD도 유의적인 증가를 보였다. 하지만 뿌리 길이와 뿌리의 건물중은 대조구와의 차이를 보이지 않았다. 과실 품질평가에서 AGH-Ringer는 대조구보다 과장의 변화는 보이지 않았지만 과 폭의 증가를 통해 과실의 무게가 과실 1개당 1.5 g이 증가하였다. 수확량에서도 AGH-Ringer에 의해 딸기 1묘당 수확량이 대조구에 비해 3.2개 더 수확하였으며 3.37 g 더 무거웠다. 딸기 품질평가 결과에서도 AGH-Ringer는 대조구 대비 과실의 색을 더욱 붉게 변화시키고 경도는 15.5% 증가시켰다. 하지만 가용성 고형물과 산 함량에는 영향을 주지 않았다. 상기와 같은 결과로 볼 때 '죽향' 딸기의 수경재배시스템에서 AGH-Ringer의 관주는 대조구로 사용된 무기 미네랄 양액비료보다 적은 미네랄 함량과 배양액 EC를 공급하고도 잎의 생육과 더불어 과실의 무게와 수확량을 증가시키고 가용성 고형물 증가를 통한 품질향상을 나타내었다. 결과적으로 본 연구결과를 통해 AGH-Ringer가 관행 무기 미네랄 양액비료 보다 생장과 생육에 효과적이고 농가의 비료 사용에 편리성(비침전)을 더한 유용한 양액비료임을 현장 실증을 통해 확인하였다. TThis study investigated the influence of AGH-Ringer (chelated organic minerals liquid fertilizer) on the growth, yield, and fruit quality of 'Jukhyang' strawberry in a hydroponic system. The results revealed that AGH-Ringer group led to significantly higher plant length than that of the control group, and that leaf length and leaf width increased with increasing leaf area. In addition, the fresh and dry weights of leaves and SPAD unit, which measures leaf color also showed a significant increase with AGH-Ringer. However, the length and dry weight of roots did not show significant differences when compared to the control group. In the fruit quality analysis, AGH-Ringer group did not show any difference in fruit length compared to the control group, but it increased both the fruit weight, which, along with the simultaneous increase in fruit width, increased the yield per strawberry. Furthermore, AGH-Ringer group strengthened the red skin color of the fruit, and resulted in significantly greater hardness than that of the control group, but did not have any effect on the soluble solid content and acidity. As shown in the results, the AGH-Ringer group resulted in lesser mineral content and lower to medium EC (electrical conductivity) in comparison to the inorganic mineral hydroponic fertilizer, which was used as a control. However, it increased the leaf growth, fruit weight, and yield, and improved the fruit quality by increasing the soluble solid content in the hydroponic system of 'Jukhyang' strawberry. Therefore, based on this study, AGH-Ringer is thought to be a more effective hydroponic fertilizer than the inorganic mineral hydroponic fertilizer. Moreover, the field experiment demonstrated that AGH-Ringer is a useful hydroponic fertilizer convenient for application in farms. This study also proved that AGH-Ringer is a fertilizer that can promote plant nutrition by controlling the salinity of the soil and facilitating the absorption of necessary minerals in future soil cultivation, thereby providing basic data for organic farming.

Guanosine 5'-monophosphate-chelated calcium and iron feed additives maintains egg production and prevents Salmonella Gallinarum in experimentally infected layers

노혜지,김희경,허수정,조향현,고홍범 대한수의학회 2017 Journal of Veterinary Science Vol.18 No.3

We evaluated the effects of guanosine 5′-monophosphate (GMP)-chelated calcium and iron (CaFe-GMP) on health and egg quality in layers experimentally infected with Salmonella Gallinarum. In this study, a CaFe-GMP feed additive was added to a commercial layer feed and fed to layers over a four-week period. All were inoculated with Salmonella Gallinarum. Body weight, mortality, clinical symptoms, and poultry production including feed intake, egg production, egg loss, and feed conversion rate were observed, and Salmonella Gallinarum was re-isolated from the liver, spleen, and cecum of the layers. All tested internal organs for the CaFe-GMP additive group exhibited significantly lower re-isolation numbers of Salmonella Gallinarum and less severe pathological changes than those in the control group, indicating that the CaFe-GMP feed supplement induced bacterial clearance and increased resistance to Salmonella Gallinarum. Additionally, due to the inhibitory action of CaFe-GMP on the growth of Salmonella Gallinarum, the CaFe-GMP additive group exhibited better egg production, including a higher laying rate and fewer broken eggs. The results suggest that a 0.16% CaFe-GMP additive may help prevent salmonellosis in the poultry industry.

Chitosan을 기질로 하는 설사 치료 천연항생제 개발 1. 장독소 대장균증 자돈 설사 치료효과 연구

노혜지 ( Hye-ji Noh ),김희경 ( Hee Kyong Kim ),조향현 ( Hyang Hyun Cho ),고홍범 ( Hong Bum Koh ) 한국키틴키토산학회 2016 한국키틴키토산학회지 Vol.21 No.1

The purpose of this research is to investigate activation of antibiotics toward livestock disease E. coli causing clinical disease and to apply OCHT to animal medicine for piglets diarrhea, by using OCHT made by raw material of chitosan. To solve the problem, after the production of OCHT controlled for the superior antibiotics, we selected OCHT with optimal physical state according to the laboratorial anitibiotics test. Lastly,the chosen OCHT is tried to be tested toward the piglets with diarrhea.Firstly, for the production of OCHT containing the optimized antibiotics, 3 types of OCHT which is divided according to the possession of molecule scope from oligosaccharids 1 to 6 (OCHT-1), 7-14 oligosaccharides range (OCHT-2), and 1-16 oligosaccharids range (OCHT-3) after disassembling raw material Chitosan with 96% of DAC into Chitosanase is prepared. And the OCHT is used for testing validity of laboratory and field. After the comparison among the laboratorial antibiosis toward the 26 types of E. coli based on different blood serum seperated form piglets with diarrhea. Therefore, OCHT-3 is finally chosen for the manufacturing convenience for the later product manufacture.In the concentration scope between 0.0001~0.5% of chosen OCHT-3, the density of E. coli of OCHT-3 significantly decreased when it is touched by E.coli type, in 1 hours regardless of density. After 12~24 hours, no gathering of E. coli was shown. (p<0.01). For the field applicability test on piglets with diarrhea toward OCHT-3, the assassement on the curing effect had been done 3 times a day (morning, noon and evening) for 7 days through the oral injection. It took maximum 4 days for treatment and the amount of OCHT for curing is minimum 2 mg to maximum 7.5 mg. Moreover, the dose for prevention is within 0.0001~1% (p<0.01). Therefore, OCHT is proved to be a natural antibiotics showing superior antibacterial and curing effect, regardless of the type of E. coli which shows differences on synthesized antibiotics tolerable germ and intestine toxic (p<0.01). In conclusition, OCHT (Product Name: EPECTROL-3) is a natural polysaccharose not showing the negative point of synthesized antibiotics as tolerance. In terms of antibiotics, OCHT contains selective molecule amount and physical properties. Also, OCHT was expected to be a natural antibiotics which can be applied to the stock raising industry.

TiFe금속간 화합물의 Zr과 Ce첨가와 냉각속도에 따른 응고 조직 변화 및 기계적 특성

노혜인,최창완,이승훈 한국주조공학회 2019 한국주조공학회지 Vol.39 No.2

Microstructural and corresponding hardness changes of TiFe compounds with Zr (0~6 at%) or Ce (0~3 at%) were studied using samples prepared at different solidification rates. In arc-melted (TiFe)-Zr samples, the Fe23 Zr6 and (Ti, Zr)2Fe phases formed in the TiFe matrix, while in the (TiFe)-Ce sample, the CeO2 phase formed along the grain boundary of the TiFe matrix. As the Zr content was increased, the volume fractions of the Fe23 Zr6 and (Ti, Zr)2Fe phases increased, forming a network structure. Accordingly, the hardness values of the samples also increased. With a small addition of Ce of approximately 0.1 at%, the as-cast microstructure could be effectively refined, reducing the average grain boundary diameter from ~100 µm to ~14 µm. In the rapidly solidified sample prepared through a melt-spinning method, the constituent phases were identical to those of the arc-melted samples while the grains were refined. The microstructural changes of TiFe alloys can affect the hydrogen storage ability as well as the mobility of the hydrogen atoms in the alloys.

시공단계 BIM모델의 적정표현수준 정의를 위한 기초 연구

노혜라(Hae-ra Noh),김지윤(Ji-yun Kim),이다운(Da-un Lee),윤석헌(Seok-heon Yun) (사)한국CDE학회 2016 한국CDE학회 논문집 Vol.21 No.4

As the needs for the mega-sized, high-rise and irregular shape of building increase, domestic application of BIM in construction industry is increasing in recent years. In this circumstance, interests in BIM modeling and modeling detail level have been also increased. BIM that is based on a three-dimensions, requires a specific and precise building expressions. And model expression level required for each phase such as design, construction, and maintenance of the construction project could be different as its needs. Because that it is not clear to define the criteria for the expression level of construction BIM model, It is difficult to decide how detail and what to model is appropriate in the practical application of BIM model. It could be the reason why the productivity in construction project became lower and lower. In this study we consider the way how to improve the productivity of BIM and analyze the appropriate representation level of construction BIM model through the experts’ survey. The results of study could suggest the basis for an appropriate expression level of construction BIM model.

사가르(Sagart)의 《상고 중국어의 어근(The Roots of Old Chinese)》 중 어휘의 어원(4)

盧慧?(번역자),申?哲(번역자),卞志源(번역자),申世利(번역자) 중국어문논역학회 2020 中國語文論譯叢刊 Vol.0 No.46

본고는 사가르(Sagart, 1999)의 《상고 중국어의 어근(The Roots of Old Chinese)》의 후반부 제22장 2절에서 제23장 2절까지를 번역한 것이다. 제22장 2절 이하는 농업 중 벼, 밀, 밭에 대한 내용을 담고 있으며, 제23장은 콩류와 차 등 기타 재배 식물에 대한 내용을 담고 있다. This paper translates chapter 22.2-23.2 of The Roots of Old Chinese by Laurent Sagart (1999) in Korean. Here translates chapter 22.2 rice, chapter 22.3 wheat, 22.4 field, chapter 23 other cultivated plants with 23.1 beans, and chapter 23.2 tea.

Sialic Acid를 지표성분으로 하는 유청가수분해단백분말의 기능성식품 개발연구 - I. 효소분리로 7% Siailc Acid가 표준적으로 함유된 유청가수분해단백분말(7%)의 랫드를 이용한 90일 반복경구투여 독성시험 평가 연구 -

노혜지 ( Hye-ji Noh ),조향현 ( Hyang-hyun Cho ),김희경 ( Hee-kyong Kim ) 한국유가공기술과학회 2016 Journal of Dairy Science and Biotechnology (JMSB) Vol.34 No.2

본 시험은 sialic acid가 7%를 함유하도록 제조한 유청가수분해단백분말제제(whey protein of hydrolysis)의 기능성 식품원료로 개발을 위한 동물안전성을 평가함에 연구목표를 두었다. GMP를 원료로 제조한 시험물질은 sialic acid 7%(v/v)와 원료인 GMP 가수분해 단백질이 93%로 구성되어 있었다(시험명: 7%-GNANA). 시험물질의 독성 유무는 한국식품의 약안전청(KFDA, 2014)과 OECD(2008)의 의약품 등의 독성시험 기준에 따라 실시하였다. 평가방법으로서, 시험물질의 투여용량을0, 1,250, 2,500 및5,000 mg/kg/day로하여SPF Sprague-Dawley 계열 암수 랫드에 90일 동안 반복경구투여하였을 때 나타나는 독성 여부를 평가하였다. 평가항목으로서는 사망률, 일반증상관찰, 체중 변화, 사료섭취량 측정, 안검사, 요검사, 혈액학적 및 혈액생화학적 검사, 부검 시 장기의 중량측정, 부검 시 육안적 검사 및 조직병리학적 검사 등을 평가하였다. 90일 반복경구투여 실험결과로서, 시험물질투여 및 관찰기간 동안 사망동물은 발생하지않았다. 또한 일반증상, 체중 변화, 사료섭취량, 안과학적 검사, 요검사 그리고 혈액학적 및 혈액이화학적 이상 및 혈액응고검사에서 대조군 대비 특이한 변화는 관찰되지 않았다(p<0.05). 부검 및 병리조직학적 평가 결과, 암수 모두에서 시험물질-유래 중요한 변화 없이 시험물질-유래 경미한 변화(nonadverseeffect)만인 5,000 mg/kg/day에서 확인되었다. Weightbasedclassification(독성 강도에 따른 분류)을 적용한 최종 독성평가 결과, 수컷의 경우 NOEL(No Observed Effect Level)은 5,000 mg/kg/day 그리고 암컷의 경우는 NOAEL(No ObservedAdverse Effect Level)은 5,000 mg/kg/day로 최종 확인되었다. 따라서, 암수 모두에서 시험물질의 NOAEL은 투여최대용량인 5,000 mg/kg/day로 확인되었다. 결론적으로, GMP를 원료로 하여 제조한 7%-GNANA(유청가수분해단백분말)는 투여가능 최대용량에서도 독성이 없는 안전한 천연물이라는 것을 확인하였고, 의약품이나 기능성 식품으로서의 개발 가능성을 확인하였다. We herein performed animal safety assessment in accordance with Good Laboratory Practice (GLP) regulations with the aim of developing sialic acid from glycomacropeptide (hereafter referred to as “GMP”) as an index ingredient and functional component in functional foods. GMP is a type of whey protein derived from milk and a safe food, with multiple functions, such as antiviral activity. A test substance was produced containing 7% (w/w) sialic acid and mostly-hydrolyzed whey protein (hereafter referred to as “7%-GNANA”) by enzymatic treatment of substrate GMP. The maximum intake test dose level was selected based on 5,000 mg/kg/day dose set for male NOEL (no-observed-effect-level) and female NOAEL (no-observedadverse- effect-level) determined by a dose-range finding (DRF) test (GLP Center of Catholic University of Daegu, Report No. 15-NREO-001) that was previously conducted with the same test substance. To evaluate the toxicity of a repeated oral dose of the test substance in connection with the previous DRF study, 1,250, 2,500, and 5,000 mg/kg of the substance were administered by a probe into the stomachs of 6-week-old SPF Sprague-Dawley male and female rats for 90 d. Each test group consisted of 10 male and 10 female rats. To determine the toxicity index, all parameters, such as observation of common signs; measurements of body weight and food consumption; ophthalmic examination; urinalysis, electrolyte, hematological, and serum biochemical examination; measurement of organ weights during autopsy; and visual and histopathological examinations were conducted according to GLP standards. After evaluating the results based on the test toxicity assessment criteria, it was determined that NOAEL of the test substance, 7%-GNANA, was 5,000 mg/kg/day, for both male and female substance, as compared with the control group, with respect to general symptoms, body weight changes, food consumption, ophthalmic examination, urinalysis, hematological and serum biochemical examination, and electrolyte and blood coagulation tests during the administration period (P<0.05). As assessed by the effects of the test substance on organ weights, food consumption, autopsy, and histopathological safety, change in kidney weight as an indicator of male NOAEL revealed up to 20% kidney weight increase in the high-dose group (5,000 mg/kg/day) compared with the change in the control group. However, it was concluded that this effect of the test substance was minor. In the case of female rats, reduction of food consumption, increase of kidney weight, and decrease of thymus weight were observed in the high-dose group. The kidney weight increased by 10.2% (left) and 8.9% (right) in the high-dose group, with a slight dose-dependency compared with that of the control group. It was observed that the thymus weight decreased by 25.3% in the high-dose group, but it was a minor test substance-associated effect. During the autopsy, botryoid tumor was detected on the ribs of one subject in the high-dose group, but we concluded that the tumor has been caused by a naturally occurring (non-test) substance. Histopathological examination revealed lesions on the kidney, liver, spleen, and other organs in the low-dose test group. Since these lesions were considered a separate phenomenon, or naturally occurring and associated with aging, it was checked whether any target organ showed clear symptoms caused by the test substance. In conclusion, different concentrations of the test substance were fed to rats and, consequently, it was verified that only a minor effect was associated with the test substance in the high-dose (5,000 mg/kg/day) group of both male and female rats, without any other significant effects associated with the test substance. Therefore, it was concluded that NOAEL of 7%-GNANA (product name: Helicobactrol) with male and female rats as test animals was 5,000 mg/kg/day, and it thus was determined that the substance is safe for the ultimate use as an ingredient of health functional foods.

Chitosan을 기질로 하는 설사 치료 천연항생제 개발 2. 말 분변에서 분리된 다제내성 대장균에 대한 철-키토산 유기태화 제재(Fe-OCHT)의 항균효능

노혜지 ( Hye-ji Noh ),김희경 ( Hee Kyong Kim ),송하연 ( Ha-yeon Song ),김준영 ( Joon Young Kim ),김대현 ( Dae Hyun Kim ),조향현 ( Hyang Hyun Cho ),고홍범 ( Hong Bum Koh ) 한국키틴키토산학회 2016 한국키틴키토산학회지 Vol.21 No.1

A total 114 E. coli isolates from 73 samples during the period from 2012 to 2013 were obtained. Isolates were confirmed asbeing multidrug resistance (MDR) bacteria isolates showed resistance. Resistance to penicillins was found in penicillin (100%), ampicillin (88%), amoxicillin (89%), oxacillin (100%), ticarcillin (53%), aztreonam (52%), ticarcillin/clavulanic acid (56%) and imipenem (33%) of isolates. The blaTEM genes were detected in 100 (100%) of the 100 ampicillin-resistant isolates. We screened for antimicrobial effect of organic iron mixed chitosan oligosaccharide (Fe-OCHT, Product Name: EPECTROL-3) on multidrug-resistance isolates associated with multidrug resistance in E. coli from feces (73 samples) on mares. We evaluated the antimicrobial activity of organic iron mixed chitosan oligosaccharide against 3 multidrug-resistance E. coli which showed resistance over 15 different antibiotics. The chitosan oligosaccharide (OCHT) inhibited the growth of multidrug resistance E. coli isolates at a concentration of 1 μg/ml in 24 hours. Also, Fe-OCHT showed a significant antibacterial activity against multidrug resistance E. coli isolates at a concentration of 100 μg/ml in 5minutes. Fe-OCHT showed better antibacterial activity than the OCHT. Consequently, we confirmed that Fe-OCHT significantly inhibited multidrug resistance E. coli isolates and might be selected as a more effective natural antibiotics for diarrheal treatment.

화학비료 대체형 유기태화 비료(Organo-Mineral Fertilizer) 개발키토산을 기질로 제조된 전용양액비료 OCHT-F의 상추(Lactuca sativa L.)를 대상으로 하는 비료효능평가

노혜지 ( Hye Ji Noh ),조향현 ( Hyang Hyun Cho ),이철규 ( Chul Kyu Lee ),김희경 ( Hee Kyong Kim ) 한국키틴키토산학회 2016 한국키틴키토산학회지 Vol.21 No.2

본 연구는 화학전용양액 비료류의 단점을 해결할 수 있는 유기태 비료(Orgnano-Mineral Fertilizer) 개발에 목표를 두었으며, 이종 유기태 비료류의 현장시험전 기초자료를 확보하기하여 본 연구를 진행하였다. 시험비료류는 화학비료인 Ringer-F와 Chitosan-oligosaccharide(OCHT)을 기질로 하여 제조한 유기태 비료로서 OCHTF를 ㈜메디뉴트롤사에서 공여 받아 사용하였다. 시험비료류의 미네럴류 및 NPK(9.2:3.78:30.5) 함량은 동일하게 조성하였다. 시험은 상추묘(Lactuca sativa L., Jeok Chi Ma)를 하우스의 토경에서 25주를 1개 시험군으로 설정하고 총 69일동안 관주시비법으로 시험하였다 시험군 조성은 관행처리구 1개군 그리고 시험비료류의 농도별 3개군으로 하여 총 7개 시험군을 설정하였다. 처리방법으로서, 일반수만을 공급한 관행재배구와 시험비료류를 1,000배(w/v, 고농도 처리군), 2,000배 및 4,000배로 희석한 후 상추묘 1주에 대하여 1주일 간격으로 50mL씩을 시비하였다. 평가방법은 상추묘 정식 후 11일과 최종69일째에 기초생장조사, 추대억제, 조직치밀도, 엽록소 변화 및 총수확량 등을 조사하였으며, 지온변화는 7일간격으로 확인하였다.결과로서, 지온은 20oC에서 31oC까지 증가함과 동시에 추대현상이 유발되는 전형적인 고온조건의 패턴을 보였다. 총수확량은 관행재배구 대비 Ringer-F는 118~122%, OCHT-F는 107~118% 범위의 증수효과를 보였다. 최적 사용농도는 초기생장단계에서 시험비료 모두에서 4,000배였으며, 후기생장 및 수확량 증가 관련 농도는 Ringer-F는 전체농도에서 그리고 OCHT-F는 4,000배가 농도의존성 보이면서 가장 효과적이였다. 상추의 조직치밀도는 관행재배구(5.18%) 대비 Ringer-F는 저농도에서 116% 그리고 OCHT-F의 경우는 중농도 이하에서 110%가 증가하였다. 미네럴류 생체흡수 및 이용성에 있어Ringer-F는 초기보다는 후기생장단계에서 그리고 OCHT-F는 전체 생장단계에서 균일성 있는 효과를 보였다. 추대억제는 시험비료류 전체에서 다소 차이는 보였으나 유의성은 보이지 않았다(p<0.05). 따라서, 키토산을 기질로 하여 제조한 유기태 비료는 화학비료와 비료효능에서 유의적으로 동등성을 보이는 것으로 평가되었다(p<0.05). The present study has conducted a fertilizer equivalency test for 10 weeks under the condition of soil cultivation in a facility house, targeting lettuce (Lactuca sativa L, Jeok Chi Ma) with OCHT-F as a test fertilizer, which has been manufactured by chelating essential minerals with Chitosan-oligosaccharide (Hereinafter “OCHT”) substrate to solve the demerit of existing chemical fertilizers (declination of body absorption capability, cohesion and sedimentation out of ion bonding, necessity of nutrient solution supply system, accumulation of soil salt and environmental contamination). OCHT-F has been manufactured with a type of powder compound fertilizer to have chemical fertilizer Ringer-F, fertilizer composition and NPK content to be 9.2%: 3.78%: 30.5%. As for its characteristic, it has non-sedimentary and water-soluble pattern and its molecular weight is 500 or less. It secures an optimum physical property regarding the root absorption of minerals and an enhancement of bioavailability. OCHT-F can be mixed with general water immediately without an exclusive nutrient solution system to secure the convenience to fertilization. A simultaneous fertilization was possible for soil drench and foliar or foliar.soil drench. In addition, sedimentation has not occurred by chemical reaction at dissolution different from chemical fertilizer. It was the range of a general exclusive nutrient solution fertilizer with 1,000~4,000 times at use. In evaluation regarding safety, the safety of Ringer-F and OCHT-F has been confirmed in entire concentration range (p<0.05). As a result of onsite evaluation on OCHT-F, its superiority has been confirmed which shows the effect of growth stimulation in both aerial part and rhizosphere portion not only within normal (21 o C) temperature of soil but also within high (25~31 o C) temperature range along with the strengthening of tissue density (control: 5.18%, Ringer-F: 5.11~6.04%, OCHT-F: 4.77~5.61%) and increasing effectRinger-F: 118~122%, OCHT-F: 104~118%) of high harvest (p<0.05). Conclusively, it has been confirmed that OCHT-F manufactured with Chitosan as a substrate procures an excellent efficiency of fertilizer that increases growth and harvest simultaneously solving the existing problems of chemical fertilizers such as the sedimentation phenomenon, the body absorption capability of minerals and the installation and use of exclusive nutrient solution system.