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Adsorption isotherms of caffeine on molecular imprinted polymer
노경호,Yinzhe Jin,최대기 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.4
A molecular imprinted polymer (MIP) using caffeine as the template and methacrylic acid (MAA) as the functional monomer was prepared. Acetonitrile was used as the porogen with ethylene glycol dimethacrylate (EGDMA) as the crosslinker and 2,2'-azobis(isobutyronitrile) (AIBN) as the initiator. By a linear and nonlinear regression analysis, the experimental parameters in the equilibrium isotherms were estimated. Then, the linear and quadratic equations for concentration and sorbents to adsorption amounts were expressed, and the adsorption equilibrium data were also correlated into the Freundlich isotherm model. Comparisons of caffeine adsorption isotherm on C18 particles as well as the molecular imprinted polymer were made. The results showed that the caffeine-imprinted polymer showed extraordinarily higher adsorption ability than C18 particles
노경호,어영,김재석,김한성,신동훈,송원근 연세대학교의과대학 2008 Yonsei medical journal Vol.49 No.1
Purpose: Coexistence of different classes of β-lactamases in a single bacterial isolate may pose diagnostic and therapeutic challenges. We investigated a spread of Klebsiella pneumoniae isolates co-producing an AmpC β-lactamase and an extended- spectrum β-lactamase (ESBL) in a university hospital. Materials and Methods: Over a three-month period, a total of 11 K. pneumoniae isolates, which exhibited resistance to cefotaxime, aztreonam, and cefoxitin, were isolated. These isolates showed positive to ESBLs by double disk tests. Minimal inhibitory concentrations (MICs) were determined by broth microdilution testing. All isolates were examined by isoelectric focusing, PCR and sequence analysis to identify blaSHV and blaDHA, and molecular typing by pulsed-field gel electrophoresis (PFGE). Results: All 11 isolates were highly resistant (MIC, ≥128 μg/ml) to ceftazidime, aztreonam, and cefoxitin, while they were susceptible (MIC, ≤2 μg/ml) to imipenem. The blaSHV-12 and blaDHA-1 genes were detected by PCR and sequence analysis. PFGE revealed a similar pattern in 10 of the 11 strains tested. Conclusion: This is the first outbreak report of K. pneumoniae in Korea which co-produced SHV-12 and DHA-1 β-lactamase, and we suggest a clonal spread of multidrug-resistant K. pneumoniae at a hospital.