개의 정형외과 수술 후 재활치료 효과

강효민,남상섭,Kang, Hyo-Min,Nahm, Sang-Soep 대한수의학회 2022 大韓獸醫學會誌 Vol.62 No.2

Rehabilitation treatments after orthopedic surgery promote postoperative healing in humans. In veterinary medicine, there is increased interest in rehabilitation treatments because they are potentially beneficial to animals in the post-operation period. This study examined the effectiveness of rehabilitation treatment in dogs that underwent orthopedic surgeries, including femoral head & neck ostectomy (FHNO) and medial patellar luxation treatment (MPLT). The group that received the rehabilitation treatment after FHNO (1.88 weeks) showed a significantly shorter recovery length of 6.62 weeks compared to those that did not receive the treatment (8.50 weeks). The other group that received the rehabilitation treatment after MPLT (4.38 weeks) showed a significantly shorter recovery length of 5.01 weeks compared to those that did not receive the treatment (9.39 weeks). For the qualitative evaluation, the types and frequencies of rehabilitation treatments were monitored. The rehabilitation programs used frequently were heat therapy, laser therapy, passive range of motion, exercise therapy, and aquatic therapy. A standard rehabilitation program after hindlimb surgery was suggested based on the quantitative and qualitative investigation. The results add additional evidence that showed the beneficial effects of rehabilitation treatments in dogs.

랫드 궁둥신경에서 말이집틈새의 형성과 Neurofascin 발현 연관에 대한 면역세포화학적연구

최혜영,조익현,이종환,남상섭,장병준,Choi, Hye-Young,Cho, Ik-Hyun,Lee, Jong-Hwan,Nahm, Sang-Soep,Chang, Byung-Joon 대한수의학회 2009 大韓獸醫學會誌 Vol.49 No.4

Neurofascin, one of the members of L1CAM, has been known to have some important roles during the development of nerve fibers. In order to investigate the role of neurofascin associated with the formation of Schmidt-Lantermann incisure in the sciatic nerve, the localization of neurofascin was studied with electron microscopy, immuno-fluorescence and immuno-electron microscopy. In the electron microscopy, the first formation of Schmidt-Lantermann incisure was checked at postnatal day 6 and the complete form of incisures traversing the whole myelin sheath began to be observed at postnatal day 8. In the immunofluorescence, neurofascin immunoreactive Schmidt-Lantermann incisures were first checked at postnatal day 6 and dramatically increased with aging by postnatal day 56. In the immunoelectron microscopy, neurofascin immunoreactive gold particles at the incisure forming sites were first observed at postnatal day 6 and the number of gold particles was increased as the animal was getting old by postnatal day 56. According to the present study, neurofascin is likely to have some relationships with Schmidt-Lantermann incisure formation.

랫드 궁둥신경에서 155 kd Neurofascin의 발현개시에 대한 면역세포화학적연구

원희영,조익현,이종환,남상섭,최농훈,박재형,장병준,Won, Hee-Young,Cho, Ik-Hyun,Lee, Jong-Hwan,Nahm, Sang-Soep,Choe, Nong-Hoon,Park, Jae-Hyung,Chang, Byung-Joon 대한수의학회 2012 大韓獸醫學會誌 Vol.52 No.3

Neurofascin, one of the members of L1CAM, has been known to have some important roles during the development of nerve fibers. In order to investigate the role of neurofascin associated with the development of nerve fibers in the rat sciatic nerve, the initial development of NF155 in the paranode was studied with immuno-fluorescence and immuno-electron microscopy. The result of the present study showed NF155 was not detected in the fetal sciatic nerve and began to reveal at the postnatal day 0 (P0) and dramatically increased by time lapse until postnatal day 7 (P7). NF155 was prominently localized in the axolemma of paranode and not detected in the central region of node of Ranvier. According to the present study, NF155 is likely to have some relationships with the formation of paranode and myelin sheath.

국내 수의과대학생의 스트레스 경험 연구

김재은,주설아,천명선,이동혁,남상섭,Kim, Jae Eun,Joo, Seola,Chun, Myung-Sun,Lee, Donghyuck,Nahm, Sang-Soep 대한수의학회 2020 大韓獸醫學會誌 Vol.60 No.2

The quality of a veterinary service depends on the veterinarian's physical and mental health. However, a veterinarian's mental health is generally considered to be more vulnerable than that of other health care professionals. As many veterinary students will enter the veterinary profession in near future, it is necessary to investigate their psychological status. This study examined stressors affecting student life in one veterinary school. Anxiety, depression, and stress levels were assessed using questionnaires and qualitative research techniques. The results obtained from questionnaires showed that the proportion of veterinary students with psychological distress was higher than expected. The major stressors experienced by the veterinary students were academic, economic, and environmental. Qualitative study using a photovoice method revealed that the students in this study felt relatively deprived compared with those in other veterinary schools. In addition, they were distressed by the school environment and limited human relationship. Our results suggest that veterinary school administrators should be aware of students' psychological distress and should undertake systematic improvement in their educational system by altering counseling programs, curricula, and school culture principles.

동물생명공학 : 도축되는 한우 혈액에서 회수한 혈청의 생물학적 안전성 분석

김민수 ( Min Soo Kim ),민경호 ( Kyung Ho Min ),최인호 ( In Ho Choi ),김지희 ( Ji Hoe Kim ),유지은 ( Ji Eun Yu ),남상섭 ( Sang Soep Nahm ) 한국동물자원과학회 ( 구 한국축산학회 ) 2012 한국축산학회지 Vol.54 No.1

본 연구에서는 성체 한우의 도축과정 중에 채혈된 혈액으로부터 분리 정제한 혈청의 생물학적 안전성을 검증하기 위하여 세균, 마이코플라즈마 및 바이러스의 검출과 동정을 실시하였다. 검사를 실시한 대부분의 혈청에서 전염성 병원체가 검출되지 않았고 두 개의 혈청에서 토양 유래의 것으로 추정되는 Bacillus thuringiensis가 검출 되었다. 본 연구의 결과는 국내에서 도축되는 한우는 생물학적 안전성을 담보할 수 있는 최소한의 기본적인 안전장치를 통해 도축된다는 사실을 보여주며 한우의 도축과정에서 부산물로 얻을 수 있는 혈액을 안전한 생물학적 제제로 개발할 수 있다는 가능성을 보여주는 것으로 생각된다. Bovine serum contains various nutrients and growth factors that can be potentially used in biological experiments, drug manufacturing process and food industry. However, almost all the bovine blood has been wasted during slaughter process in Korea, thus there is a high demand for alternative uses of the wasted sera. In order to produce high quality and safe sera, it is necessary to screen zoonotic pathogens as well as other microbial contaminants to prevent any downstream contamination. The present research has been undertaken to assess biological safety of Hanwoo sera by determining microbiological contamination during slaughtering and handling processes. Serological tests have been performed to detect bacteria, mycoplasma and virus contamination in total of 52 Hanwoo sera. No sera were found to be contaminated with mycoplasma or virus, but only two sera were found to be contaminated with Bacillus thuringiensis. The present result shows that Hanwoo sera obtained from slaughtering process are biologically safe and have potentials to be developed as a biological reagent. Moreover, the methods employed in our study may provide basic standard for microbiological screening methods once wasted Hanwoo sera gain industrial values.

저병원성 조류인플루엔자 감염에 따른 육계의 면역인자 변화

김덕환(Deok-Hwan Kim),김규직(Kyu-Jik Kim),노진용(Jin-Yong Noh),이선학(Sun-Hak Lee),송창선(Chang-Seon Song),박혜경(Hae Kyoung Park),남상섭(Sang-Soep Nahm) 한국가금학회 2020 韓國家禽學會誌 Vol.47 No.4

가금산업에서 전염성 질병은 농장의 생산성에 악영향을 미치는 중요한 요인이다. 그 중 저병원성 조류인플루엔자는 산란율과 증체율에 영향을 준다. 본 연구에서는 육계에서 저병원성 조류인플루엔자 감염시 혈청 단백질 및 사이토카인의 변화 양상을 조사하였다. 1일령의 육계를 1주령부터 4주령까지 각 주령마다 저병원성 조류인플루엔자를 공격 접종 후 혈중 chDBP, Chicken Ovotransferrin, chIL-1β, chIL-6, chIFN-γ의 양을 비교하였다. 어린 주령에 저병원성 조류인플루엔자를 공격접종한 그룹에서는 4주령에 음성대조군과 비교해 chIL-1β, chIL-6가 유의성 있는 낮은 값을 보이는 것을 확인하였다. 추가적인 연구를 통하여 chIL-1β, chIL-6가 질병의 경과를 확인할 수 있는 보조적인 감염 지표로 활용될 수 있을 것으로 판단된다. Avian influenza virus infection, one of the most important diseases recognized in the poultry industry, is known to cause changes in cytokine and serum protein levels. However, the normal ranges and/or age-dependent changes in important cytokines and serum proteins associated with influenza infection have not been fully elucidated. In this study, the levels of cytokines (interleukin-1β, interleukin-6, and interferon-γ) and serum proteins (vitamin D binding protein and ovotransferrin) were determined in 1-week- to 4-week-old broilers at 1-week intervals after challenge with a low pathogenic influenza virus. The results showed that the physiological levels of cytokines and serum proteins varied with aging during the 4 weeks. The levels of interleukin-1β and interleukin-6 increased from 20% to 35% after influenza infection compared to those in the negative control group, indicating that these cytokines may be used to monitor disease progression.