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스트레스 상태의 고양이에서 lipopolysaccharide 투여가 혈중 감마글로불린 생산에 미치는 영향
나기정,이창우 한국임상수의학회 2000 한국임상수의학회지 Vol.17 No.2
The activated immune systems with lipopolysaccharide(LPS) were evaluates by ${\gamma}$- globulin levels in domestic cats. Especially, cats were in a stress condition, which was unfamiliar environmental change. Effect of continuous unfamiliar environmental stressor was a diminution of production of serum total globulin auld ${\gamma}$- globulin on feline infectious peritonitis(FIP) vaccination. The ${\gamma}$-globulin production on FIP vaccination more increased in LPS treated 77ups than non-LPS treated group. Also. the ${\gamma}$-globulin production on FIP vaccination was maintained in LPS treated group under stress condition. These results imply that that the ${\gamma}$-globulin can be produced under a stress condition by mitogen like as LPS.
Jn vitro에서 cortisol이 개 말초혈액 단핵구세포의 증식에 미치는 영향
나기정,양만표 한국임상수의학회 1997 한국임상수의학회지 Vol.14 No.2
In vitro effect of cortisol on the proliferation of canine peripheral blood mononuclear cells (MNC) was examined. The MNC was isolated from peripheral blood by a gradient centrifugation with Picoll-Hypaque. The cell proliferation assayed using a noneradioactive 5-Bromo-2'-deoxy-uridine (BrdU) kit. The MNC proliferated well in response to either phrtobeRagg$]$utinin-p (PHA-P) or culture supernatant from MNC stimulated with PHA-p. However, these proliferative responses of MNC were not affected by addition of coitisol of 1 to 1,OOfl ng/ml. The addition of cortisol in MNC culture with either PHA-P or corture supernatBnt from MNC stimulated with PHA-P far 4 days wag not also influenced on the viabilities of cultured MNC. In conclusions it was able to assay the cell proliferation with BrdU instead of radioactive isotope e.g. tritiated thymidine (3H-TdR). These results suggested that cortisol does not at least influence on MNC proliferation in vitro.
잣나무 지엽을 이용한 발효 조사료가 한우의 성장에 미치는 영향
나기정,최인규,정의배 한국임상수의학회 1999 한국임상수의학회지 Vol.16 No.2
The objective of this study was to estimate the possibility of use as a crude feed about Korean pine foliage. Foliage was fermented aerobic and anaerobic state. And then, it was fed Korean native bull 5 to 6 month old. Bulls were fed control (n=4), aerobic fermented (n=4) and anaerobic fermented (n=4) feed 30 percent of total ration. Bulls were examined with blood sample and body weight on day 0, 60, 120 and 180. There were no differences on complete blood cell count serum chemistry and incidence of disease among groups for experiment period. Also, electrolytes (Ca, Mg, P) balance were good condition. Experimental feed did not induced toxicosis in kidney and liver, Fermented foliage and leaves of Korean softwood were efficient crude feed in carbohydrate, fat and protein metabolism. Experimental feed induced growth of Korean native bull body weight in growing stage without retard. In addition to anaerobic fermented feed has a more higher growth rate than control feed. Our conclusion is that fermented softwood substitute some part of ration, minimum 30 percent, in growing stage of a Korean native bull.
ELISA를 이용한 cortisol 측정법의 정립 및 임상적 응용
나기정,이창우,Na, Ki-jeong,Lee, Chang-woo 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.3
ELISA kit for cortisol was developed and then evaluated. Polyclonal antihydrocortisone-3-(o-carboxymethyl)oxime BSA rabbit serum was used to coat the 96-well microplates. The minimum detection limit of the kit was 250pg of cortisol per milliliter. The within-run variation and the day to day variation of the ELISA system were 2.0 and 5.9 at maximum, respectively. The kit was used to determine whether salivary cortisol concentration could replace blood cortisol concentration in dexamathasone suppression test of dogs. Changes of cortisol concentration were measured in serum or saliva after intravenous administration of 0.01mg of dexamethasone per kilogram of body weight. Blood alone, saliva alone or both were collected at 0, 30, 60, 120, 240, and 360 minutes after injection of dexamethasone. The change in blood cortisol concentration was found to be suitable in dexamathasone suppression test of dogs, but the change in salivary cortisol concentration was not. The kit was also used to determine whether salivary cortisol concentration could be a stress index as well as blood cortisol concentration in dogs. Two types of trial were performed to estimate the stress either by blood or salivary cortisol concentration. The first trial was stress experiment by intravenous injection of 0.2IU of PZI-insulin per kilogram body weight. Either blood alone or saliva alone was collected at 0, 30, 60, and 90 minutes after insulin administration. Both blood and salivary cortisol concentration were found to be suitable index in estimating stress from hypoglycemia by injection of insulin. The second trial was stress experiment by electrical irritation. The dogs were irritated with anti-bark device for 10 seconds. Blood was collected before and at 2 and 5 minutes after electrical irritation. Saliva was collected before and at 3 and 6 minutes after electrical irritation. The blood cortisol concentration, but not the salivary cortisol concentration was found to be suitable index in estimating stress from electrical irritation. Cushing syndrome in a dog was also successfully diagnosed with this kit.
Morphologic and Molecular Characterization of Psoroptes ovis from Pet Rabbits in South Korea
나기정 한국임상수의학회 2024 한국임상수의학회지 Vol.41 No.2
Pet rabbits are affected by the highly contagious ectoparasite Psoroptes (P.) ovis , which carries significant economic implications for the global rabbit industry. Accurate identification of the mite species remains essential to implement effective treatment and control strategies. Two approximately one-year-old female pet rabbits were admitted to the Veterinary Teaching Hospital of Chungbuk National University due to excessive scratching of the ears and the presence of waxy debris within the ear canals. Mites were isolated from the waxy debris extracted from the ear canals and subsequently identified as Psoroptes spp. through microscopic examination. Species confirmation was achieved through mitochondrial cytochrome c oxidase subunit 1 (cox1) gene analysis. The analysis revealed the mites to be P. ovis based on cox1 gene sequences. The deposited GenBank accession numbers for these sequences are OR985022 and OR985023. This represents the first report of mitochondrial cox1 gene sequences of P. ovis isolated from pet rabbits in South Korea.