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      • KCI등재

        단감나무로부터 분리한 탄저병 병원균 Colletotrichum spp.의 RAPD와 PCR-RFLP를 이용한 유연관계 분석

        김희종,엄승희,이윤수 한국미생물학회 2002 미생물학회지 Vol.38 No.1

        Colletotrichum spp.는 광범위한 기주범위를 갖는 다범성균으로 각종작물에 피해를주는 중요한식물병원진균이다. 최근 국내에서 널리 재배되고 있는 단감, 사과, 복숭아, 포도 등에 탄저병 이 발생하여 많은 경제적 손실을 초래하고 있다. 탄저병원균의 경우 기존에는 주로 형태적 특징이나 배지 상에서의 특성, 기주에 대한 병원성의 차이에 의존하여 분류를 해 왔다. 그러나 최근에는 병원균의 분류에 있어 문제점을 해결하기 위하여 분자생물학적 방법을 이용하고 있다. 이에 본 실험에서는 Random Amplified Polymorphism DNAs (RAPD)와 Ploymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) 기법을 이용하여 단감나무에 탄저병을 일으키는 균들 간에 유연관계를 밝혔다. 유연관계 분석결과 크게는2개의 그룹으로 나뉘었고 작게는5개의 그룹으로 나뉘는 것을 알 수 있었다. Colletotrichum species are important fungal pathogen that cause great damages on various host plant species worldwide. In Korea, Colletotrichum species cause massive economic losses on apple, peach, grape, and essecially, sweet persimon productions. In the past, Identification of the pathogen and the studies on the genetic relationships among the pathogenic isolates were mainly based on morphology, cultural characteristics, and the difference in pathogenicity. However, in recent years, these traditional methods have been replaced with molecular methods to solve the difficulty of classification on pathogens. Therefore, in this study, RAPD and PCR-RFLP methods were employed for the studies of genetic relationship among the different isolates of Colletotrichum species that cause damages on sweet persimon. As a results of genetic relationship analysis, Colletotrichum species tested were divided into two big groups or five small groups.

      • 水路斷面 決定에 關한 考察

        金熙鍾 東亞大學校 1963 東亞論叢 Vol.1 No.-

        In this thesis some discharge formulas have been reviewed in order to design open (river) channels. Data obtained from the Kyung-Ju Sight-Seeing Center are used for the purpose of studying the accuracy of a specific discharge formula in a natural streamlet.

      • 釜山地方의 降雨强度曲線에 對한 考察 및 洛東江流域의 D.A.D 解析

        金熙鍾 東亞大學校 1964 東亞論叢 Vol.2 No.2

        1. In this paper intensity-duration Curves are based on precipitation records of the Pusan Weather Bureau station. 50year Intensity-Duration equation: ???= 12516 ???=intensity mm/hr T+85.8 T=duration min 100year Intensity-Duration equation: ???= 13872 T+84.8 200year Intensity-Duration equation: ???= 15142 T+83.1 5. Inthis paper the curve of average precipitation per unit area in the Nak-dong Gang river basin was made by the Holton method. The mean rainfalls in the basin are measured by the basis of the Thiessen method and the Isohyetal. The equation is ???? R:average precipitation per unit area. A:basin area.

      • KCI등재

        Successful Treatment of Lead Poisoning in a Whooper Swan (Cygnus cygnus) and a Cinereous Vulture (Aegypius monachus) in Korea

        김희종,박성준,이해범,박영석,김문정,김영준 한국임상수의학회 2017 한국임상수의학회지 Vol.34 No.6

        We described the successful treatment of cases of lead poisoning in a Whooper swan (Cygnus cygnus) and a Cinereous vulture (Aegypius monachus) in Korea. The swan and vulture rescued were diagnosed with lead poisoning based on clinical signs, the presence of suspected lead-like particles in the ventriculus detected by radiography and elevated blood lead levels. Endoscopic retrieval for the swan and gastric lavage for the vulture were used to remove the lead respectively. After the removal of lead particles, a course of chelation treatment was administrated. This would be the first report of successfully treating wild birds with lead poisoning in Korea.

      • 釜山市 都心地內 駐車場의 實態調査分析

        金熙鍾,吳錫氣 동아대학교 공과대학 부설 한국자원개발연구소 1977 硏究報告 Vol.2 No.1

        A Study on parking problems in metropolitan regions in Korea is a recent concern. In case of Busan, although the master plan for traffic was established in 1974 the and survey for the parking problems were not considered. In this study, the area for this survey was limitted to 3 on-street parking places in Busan C.B.D. The data Survey was carried out for 8 hours from 9.00 A.M. to 17.00 P.M. on 15th oct. 1976. The method of this survey was performed through continuous observation and interview by each vehicles. The Contents of the survey are necessary basic data for the establishment of parking plans such as parking time, parking purpose, parking use and kinds of vehicles, etc. The results of this study are summarized as follows; ① Average parking time~61.7 min. (By the continuous observation method) ② Parking purpose Business and Commercial;88.8% ③ Method of parking use~Daily thicket use:77.1%, Monthly ticket use:22.9% ④ Major kind of vehicle~Taxi:91.1% ⑤ Average parking index~l.30 ⑥ Average rotation index~9.5.

      • 都市에서의 上水需要量의 變動特性에 대한 硏究

        金熙鍾 東亞大學校 1970 東亞論叢 Vol.7 No.-

        It is the purpose of this study to investigate into current water demands in cities and Eup's(town), and to eatablish the relationship between domestic water use and such factors as the family economics, consumers ages, and alumbing fixturer provided for the house, etc... in the areas served with in the city of pusan. Daily per capita consumption ranged from 104 litres in the cases of large municipalities, to 92 litres for small as well as medium sized cities. The little difference of per capita consumption between the large and small cities is considered attributable to the fact that in most cities, the extension of the water supply systems has not been made to meet the increased demand due to the population growth. In 1969, it was found that domestic use of the water for pusan city averaged 36.92 lpcd (litre per capita per day). Which figure are exceeded even by those corresponding to a small city in Japan. The unusally high percentage of the unaccounted for water(47, 32 70 for pusan City) is one of the major factors for difficulties in the mangement of water supply systems. The correlation coefficient derived from the relationship between daily per capita consumption and monthly per capita income for a family indicated a high value of 0.9647, and the formula representing the relationship is as follows; Y=51+0.0066x providing that y is for daily per capita consumption, lpcd; and x for monthly per capita income, in Won. Daily per capita consumption in as much as domestics use is concerned, fluctuates month to month; August sees the peak and February the lowest. The ratio of the peak to the lowest is apprxoimately 1.56 in the case of the high income group, considerably differing from those corresponding to the medium income group of 1.75 and to the low income group of 1.74. The maximum monthly per capita per day consumption figures for domestic use forthe high income group, the meidum income group and the low income group are, respectively, 1.22, 1.28 and 1.32 times of the average daily consumption figures corresponding to the respective groups. A house provided with both the flush-water closet and the bath is consuming 195.48 litres on the basis of per capita per day figures, thus exceeding the aerage per capita per day consumption for the aerage high income group. A house with the bath only uses 115.01 lpcd which is comparable to the daily per capita consumption for the medium income group of 112.171pcd A family of 45-50 age group dominating uses 144.53 lpcd. While a family in which an age group over 50 out numbers consumes an average 88.48 lpcd. the least among the different age groups.

      • 海雲台砂浜의 漂砂에 관한 硏究

        金熙鍾,姜周復,朴雲龍 동아대학교 공과대학 부설 한국자원개발연구소 1982 硏究報告 Vol.6 No.2

        It must be a significant problem of national standpoint as well as Busan city that the area of Hae un dae beach continues to diminish since 1976. This problem seems to be caused by the transportion of littoral drifts. Therefore, this paper intended to investigated parameters having best correlationship with the drift and be able to estimate transfer quantity in future, and besides to develop protection method to control the phenomena. 1. A primary factor of Haeun-dae seashore erosion is that the more wave steepness is, the more severe erosion is. When the value of slope is above 0.025 erosion occurs, and relatively accretion occurs when it is below 0.025. And other factor is that is that if constructions place near the shoreline, erosion will be more sever. So in case of landfilling the front of the mouth of Chun river, erosion seems to be more severe because shortage of supply-sand was caused by position deformation of the mouth of river. 2. The range of specific gravities of sands in Heaun-dae beach is 2.56-2.69. At No.23 a great deal of soil was involved, at No.33 clam-shell, sand soil were mixed and dam-shells were mainly included at No.44 3. The materials of sea-bed were sampled at point of 50m, 100m, 300m, 400m, 500m, away from the shoreline of No.23, No33, No.44, No.55, No.65 relatively. As a result of analysis of the specific gravities and median diameters, the specific gravities at both sides decrease according to the depth of water, and in the vicinity of the center a kind of cyclic phenomenon, which the specific gravities having the range of 2.76-2.27 increase and decrease in turns at intervals of 50m, appears. Near the center there are many clam-shell and S O₂, the component of pure sand is only 45.44%, so the specific gravities decrease to 2.27. 4. It seems that entrance and exit of hae un dae sand to Su-yeang, chun river from Song jeang line, recently it is assumed that it go to Hae un dae on the ground of insufficient evidence in diameter, specific gravity. 5. It was known that erosions happen is spring and autumn mainly. 6. Total erosion quantity is 15000m3 and the reduction area of beach sands is 2,230㎡ from July, 1981 to June, 1982 7. The equilibrium slope of beach sand had been step type untill 1982, 3. 17, but it has belonged to bar type since 1982.3, 17. 8. The specific gravities of the materials of sea-bed at the front of Kuk Dong hotel and the 3rd watchtower are about 2.70, Here, sands seems to be transported from land to sea at the latter, from west to south at the former. Chun-river is guessed to be a supply source of sands. But At the front of the 1stwatch tower, the specific gravity of sands is about 2. 57, which is relative light. considering that the specific gravity of sands is 2.573 in su-yeang river. At the front of Beach hotel the specific gravity is the smallest value of 2.54. Here, there are mainly clam-shells, so it is concluded that sands were transported from East-river「in song- Jeung. 9. In protection method of beach erosin, back sea dyke more than 10m(requirement model test) and construct 10m in width low step, is absorbed by it. It is to be desired that beach nourishment per 20,000㎥/year 10. To estimate the wave steepness and erosin quantity the equation, V= -338,727.3(H_0/L_0)+4898.2, was induced. 11. By the equation, V= -338727.3(H_0/L_0)+4898.2 total evosion quantify is 159,042㎥ from July. 79 to June, 82. Because Busan is the city surrounded by sea, it is absolutely necessary to be able to observe the deepwater wave height by means of wave height observation stations.

      • 中小河川의 洪水到達時間에 관한 硏究

        金熙鍾,閔丙亨 동아대학교 공과대학 부설 한국자원개발연구소 1975 硏究報告 Vol.1 No.1

        When the peak discharge of small and medium rivers is shown in the rational formula the mean intensity of rainfall depends on the time of concentration. Accordingly, the time of concentration should correctly be calculated in of order to obtain peak discharge with good accuracy. There is, however, no theoretical formula available, which gives the correct time of concentration, and empirical formulae are mainly used for determining it. In our country, Rziha formula has generally been use for a mountaineus region, and Kraven formula for a plain land. When these formulae mentioned above are, however, applied to small and medium rivers in Korea, the degree of accuracy obtained from these formulae is found to be low. After the authors have analysed a comparison between the measured times of concentration for small rivers in Busan area and small & medium rivers in the Nakdong basin, and the calculated values using two formulae given above together with the following formulae (1) American Society for Soil Preservation formula T_(c)=(0.868 L³/H)^(0.385) (2) Kirpich formula T_(C)=0.0001875 L^(0.77)/I^(0.385) (3) Japanese Civil Eng. Research Center formula T_(c)=1.67×10^(-3)(L/√I)^(0.7) for rural basin T_(c)=2.40×10^(-4)(L/√I)^(0.7) for urban basin, the conformity of T_(c)= m(L³/H_(0))^(n) type formula, which is obtained by I_(0)(- H_(0)/L), is found to better as a formula for the determination of time of concentration, as compared to that obtained by I(-H/L.) The formulae to be published for the time of concentration on small rivers in Busan area and small & medium revers in the Nakdong basin are as follows; 1. T_(c)(min)= 2.600(L³/H_(0))^(0.383) for small rivers in Busan area. 2. T_(c)(hr)=1.581(L³/H_(0))^0.(128) for small & medium rivers in the Nakdong basin where, L=length of river (㎞) H_(0)=2F/L(㎞) F=area between river bed line and horizontal line based at the practical target point in river profile (㎢)

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