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오호혈(五虎穴)이 족과부(足踝部) 체표온도변화(體表溫度變化)에 미치는 영향(影響)
김현중,변재영,안수기,이건목,박용현,김경식,Kim, Hyun-Jung,Byun, Jae-Young,Ahn, Soo-Gi,Lee, Geon-Mok,Park, Yong-Hyun,Kim, Kyung-Sik 대한침구의학회 2001 대한침구의학회지 Vol.18 No.1
Background and Purpose : There have been many studys about the relationship between the meridians and the acupoints of the twelve meridians, but no study about the extra points. To verify Dong-Shi acupoints, a kind of extra points, we studied the effects on the thermal changes of the ankle region following acupuncture on the Wu-Hu. Object and Methods : This study was researched into clinical statistics for 45 healthy normal students. The object was divided into two groups. One was the control group and the other was the acupuncture group. The acupuncture group was divided into two groups ; ACU-up : The class of ascent thermal change among acupuncture group. ACU-dn : The class of descent thermal change among acupuncture group. First, in the control group, we took a picture at the ankle region(BL60, BL62, GB40, ST41) without any stimulation with the Digital Infrared Thermal Imaging System and did after 10 minutes respectively. Second, in the acupuncture group, we took a picture without any stimulation and then acupuncture on Wu-Hu and took a picture after 10 minutes in the same way respectively. Results : 1. In the control group, average thermal change of BL60, BL62, GB40 and ST41 was decreased(about $0.2^{\circ}C$) after 10 minutes. 2. The skin temperature of all measured parts was significantly lower in the ACU-dn group after 10 minutes. 3. There were no significant differences of thermal change between the control and the acupuncture group, between the control and ACU-up group after 10 minutes. Conclusion : This result indicated that the acupuncture on the Wu-Hu was effective at the ankle region. And so the more study is needed continuously.
김현중,김민수,박영재,이은용,Kim, Hyun-joong,Kim, Min-soo,Park, Young-jae,Lee, Eun-yong 대한침구의학회 2004 대한침구의학회지 Vol.21 No.6
Objective : This study was performed to examine the Magnetic therapy decrease the allergic reaction of Bee Venom. Methods : We injected BV to sample group(n=10) and control group(n=10) at 2 points of body, and also sample group was treated with Magnetic therapy additionally. We observed the change of local pain, pruritus, color difference value at 1 hour, 24 hours after injection. Results : Local pain, pruritus and color difference value of sample group were more decreased than control group. Conclusions : The Magnetic therapy decreased allergic reaction of Bee Venom.
PCR을 이용한 glyphosate 저항성 콩의 검출법에 관한 연구
김현중,박선희,김해영,Kim, Hyun-Joong,Park, Sun-Hee,Kim, Hae-Yeong 한국식품과학회 2001 한국식품과학회지 Vol.33 No.5
본 연구는 유전자재조합 기술에 의해 개발된 glyphosate에 내성을 가지고 있는 콩(GTS)의 모니터링을 위하여 PCR을 이용한 검출 방법에 대한 실험을 수행하였다. Glyphosate에 내성이 있는 콩에 삽입된 유전자와 표준대조 유전자인 lectin과 ferritin 유전자를 근거로 제작된 primer와 CTAB 방법으로 추출된 콩의 DNA를 template로 이용하여 PCR을 수행하였다. GTS의 검출을 위한 제작된 primer들은 GTS와 특이적으로 반응하여 증폭된 PCR 산물을 생성하였으나, non-GTS와는 PCR 산물을 생성하지 못했다. 증폭된 염기서열 분석을 통하여 GTS에 특이적인 것을확인하였으며, 약 0.05%가 포함되어 있는 GTS까지 검출이 가능함을 보였다. A method using PCR was developed for the monitoring of glyphosate tolerant soybean (GTS) produced by the DNA recombination technique. We designed 3 pairs of specific oligonucleotide primers based on the gene sequences inserted in soybean and in lectin and ferritin genes as internal standards. Template DNAs were isolated from soybeans by the modified hexadecyl trimethyl ammonium bromide (CTAB)method and used for PCR with different primer sets. PCR, used with specific primer sets for GTS detection, showed the amplified DNA fragments with GTS template DNA but no product showed with non-GTS template. PCR amplified products were confirmed by DNA sequencing and were detected for up to 0.05% of GTS template DNA.
요통(腰痛) 환자에 대한 이침(耳鍼)의 침자수법(鍼刺手法)에 따른 치료효과(治療效果)의 임상적(臨床的) 고찰(考察)
김현중,김민수,이은용,박영재,Kim, Hyun-joong,Kim, Min-soo,Lee, Eun-yong,Park, Young-jae 대한침구의학회 2004 대한침구의학회지 Vol.21 No.1
Objective: The purpose of this study is to evaluate the effect of auricular acupuncture therapy by using different techniques combined with common acupuncture treatment on lumbago patients. Methods: 30 patients with lumbago who were hospitalized in Semyung Univ. Oriental Medical Hospital were devided into three groups. Group A of 10 patients were treated by common acupuncture treatment and auricular acupuncture therapy by methods of burying needles. Group B of 10 patients were treatedd by common acupuncture treatment and auricular acupuncture therapy by methods of pricking needles. Group C of 10 patients were treated only by common acupuncture treatment. The pain scale and improvement index of these three groups were evaluated after 10 days of admission. Results: 1. On the score of pain scale, group A and B were both superior to group C, but had no specific difference(p=0.15). 2. On the score of improvement index, group B was superior to group A, and group A was superior to group C(p=0.04). Conclusions: The combination of auricular acupuncture therapy and common acupuncture treatment was more effective than only to use common acupuncture treatment to lumbago patients. Especially, using the auricular acupuncture therapy by pricking methods, was the most effective methods in increasing improvement index.
단기간 Fluticasone Propionate 투여 용량에 따른 기저 코르티솔 농도의 변화
김현중 ( Hyun Jung Kim ),김형식 ( Hyoung Sik Kim ),이홍 ( Hong Lee ),문성기 ( Seok Tae Lim ),임석태 ( Sung Gi Moon ),박지현 ( Ji Hyun Park ),이흥범 ( Heung Bum Lee ),이용철 ( Yong Chul Lee ),이양근 ( Yang Keun Rhee ) 대한결핵 및 호흡기학회 1997 Tuberculosis and Respiratory Diseases Vol.44 No.5
Bcl-2 및 IAP family의 발현 변화와 caspase 활성을 통한 봉독의 인체폐암세포 apoptosis 유도
우현주(Hyun Joo Woo),김현중(Hyun Joong Kim),홍수현(Su Hyun Hong),홍상훈(Sang Hoon Hong),최병태(Byung Tae Choi),이용태(Yong Tae Lee),박동일(Dong Il Park),최영현(Yung Hyun Choi) 한국생명과학회 2007 생명과학회지 Vol.17 No.11
본 연구에서 봉독의 apoptosis 유도에 의한 항암기전 효능을 A549 인체폐암세포를 대상으로 조사하였으며, apoptosis 유발에 관여할 것으로 예상되는 중요한 유전자들의 발현 및 활성에 미치는 봉독의 영향을 조사하였다. A549 세포의 생존율은 봉독의 처리 농도 증가에 따라 강력하게 억제되었으며, 이는 염색질 응축 현상을 동반한 apoptosis 유발에 의한 것임을 알 수 있었다. 봉독 처리에 의한 apoptosis 유발은 Bax 및 Bcl-xL의 발현 변화 없이 Bcl-2의 발현 감소에 따른 상대적인 Bax의 발현 증가와 IAP family에 속하는 인자들의 발현 감소 및 caspase의 활성화와 연관성이 있었다. Bee venom is used to treat inflammatory diseases in Korean traditional medicine and has been known to inhibit proliferation and induce apoptosis in cancer cells. However, the molecular mechanisms involved in bee venom-induced apoptosis are still uncharacterized in human lung cancer cells. In the present study, we investigated the effects of bee venom on the apoptosis of A549 human lung epithelial cancer cells. Treatment of bee venom inhibited the cell viability and induced apoptosis in a concentration-dependent manner as measured by hemocytometer counts, fluorescence microscopy and flow cytometry analysis. Bee venom-induced apoptosis in A549 cells was associated with a marked inhibition of anti-apoptotic Bcl-2 expression without significant changes in the levels of Bax and Bcl-xL. Bee venom treatment also inhibited the levels of IAP family members such as cIAP-1 and cIAP-2 and induced the proteolytic activation of caspase-3 and caspase-9. Although further studies are needed, the present results suggest that apoptotic signals evoked by bee vemon in A549 cancer cells may converge caspases activation through a down-regulation of Bcl-2 rather than an up-regulation of Bax. These findings provide important insights into the possible molecular mechanisms of the anti-cancer activity of bee vemon in human cancer cells.