Effects of suspended solids in natural seawater on microalgal biomass productivity

김필한,박한울,이철균 한국공업화학회 2014 한국공업화학회 연구논문 초록집 Vol.2014 No.1

Suspended solids, with various sizes, in seawater, may affect algal biomass productivity or not. In the previous research, biomass productivity of microalgae was enhanced by filtering all SSs using microfiltration. In this research, the fractionated SSs by their sizes were tested to find the effect of each fraction on the growth of a microalga, Tetraselmis sp. KCTC12433BP. The SSs were fractionated by filtration with glass microfiber filters, which pore sizes were 0.2 μm (A), 0.7 μm (B), 1.2 μm (C), and 3.0 μm (D). Each fractions were put into the f/2-Si medium with natural seawater. The highest overall biomass productivity, 0.10 g/L/day, was obtained from D fraction group, and the cultures with B, C, and A fraction groups followed after. In the control group, containing all SSs, the biomass productivity was decreased by 61%. Some suspended solids, the size ranges from 1.2 μm to 3.0 μm, look as if they have advantageous effect, while the other SSs that larger than 3.0 μm look as if they have disadvantageous effect on the microalgal growth. Further studies will be on analyzing the constituents.

동적 채널 Add/drop 환경에서 광섬유라만증폭기의 이득제어알고리즘

김필한,박남규 한국통신학회 2004 한국통신학회 기타 간행물 Vol.2004 No.11

Intravital laser-scanning microscopy for real-time cellular visualization

김필한 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1

Recent advances in genomic technology have allowed a creation of animal model for human disease with genetically encoded biomarkers such as green fluorescent protein (GFP), which has opened up a new avenue to investigate complex pathophysiology of human disease in much greater details at cellular and molecular level. Over the recent years, intravital laser-scanning microscopy has demonstrated dynamic 3D visualization of various biological processes in the living subject, which provides unprecedented insights those were impossible to obtain by traditional static 2D snapshots (e.g. histopathology and cytometry). It has been utilized to monitor gene expression, protein activity, drug delivery, cell trafficking, cell interaction, physiological response under external stimuli in live animal in vivo, which provides new insights unobtainable by conventional ex vivo and in vitro observation. In this talk, recent in vivo cellular imaging studies utilizing custom-design ultrafast laser-scanning intravital microscopy system will be introduced. First, in vivo visualization of T and B lymphocyte trafficking at the high endothelial venule (HEV) of lymph node will be demonstrated. Individual endothelial cell of HEV can be clearly identified with its distinctive cuboidal morphology. Dynamic flowing behaviors of T and B lymphocytes and their dynamic migrations across endothelial cells and fibroblastic reticular cells were analyzed in vivo. Second, in vivo monitoring of small lipid and drug molecules in intestinal villi will be described. By utilizing lacteal-reporter (Prox-1-GFP) mouse, we successfully visualized transepithelial absorption of molecules across villus enterocyte, diffusion through lamina propria and subsequent transport via lacteal. Interestingly, we observed active contractile movement of lacteal in concert with villi motion, which suggests lacteal may act as an active pump during lipid absorption, not merely as a passive conduit. Moreover, we identified highly diverse pattern in absorption dynamics of various exogenous molecules as well as intrinsically fluorescent drugs through enterocytes and lacteal in vivo.

아이빔테크놀로지(주), 미래 바이오헬스 시장의 첨단 플랫폼 생체현미경 솔루션 글로벌 선도기업을 노린다

김필한 한국화학공학회 2021 NICE Vol.39 No.2

아이빔테크놀로지㈜, NIR Femto-second Laser를 이용한 세계 최소형 3차원 올인원 이광자 생체현미경

김필한 한국레이저가공학회 2022 Laser Solutions Vol.25 No.8

의생명연구를 위한 생체 내 현미경

김필한,Kim, Pil-Han 한국광학회 2013 광학과 기술 Vol.17 No.3

파장분할다중화방식 전송로의 In-service 감시를 위한 새로운 감시시스템의 구현 및 성능평가

김필한,윤호성,박남규,서재은,정기태,유기원,이규행 한국광학회 2001 한국광학회지 Vol.12 No.2

Novel supervisory system for WDM transmission link using conventional optical time domain reflectometry was presented. By modifying the structure of erbuim doped fiber amplifier to support bi-directional transmission at arDR pulse wavelength and launching the optical pulse into transmission link in the opposite direction of data signal propagation to avoid the distortion by cross-gain modulation, it is possible to monitor the WDM link in service. To prove the validity of proposed scheme, the supervision result of 2.5 Gbps $\times$ 8 channel WDM 320 km transmission system in service by arDR was presented. And power penalty due to monitoring was measured as smaller than 0.3 dB. .3 dB.

지방공무원의 정책 기대가 중앙정부신뢰에 미치는 영향:윤석열 정부 지방분권 실천과제를 중심으로

김필,박재희 한국정책분석평가학회 2024 정책분석평가학회보 Vol.34 No.4

새로운 정부가 출범할 때 발표하는 여러 정책에 대한 공무원의 기대가 정부신뢰를 높인다면 원활한 정책 집행이 이루어질 수 있다. 본 연구는 윤석열 정부의 지방분권 강화 실천과제 8개를 네 가지 유형으로 나누어 이에 대한 지방공무원의 예견적·규범적 기대가 중앙정부신뢰에 어떤 영향을 미치는지 분석하기 위해 2022년 7월 대전광역시 본청 공무원 233명을 대상으로 설문조사를 실시하였다. 분권 정책 유형의 분류는 정부분권과 주민분권 여부와 정책 구체성의 정도를 활용하였다. 방법론으로는 각 기대가 독립적으로 갖는 영향력 뿐만 아니라 두 가지 기대의 관계가 정부신뢰에 미치는 영향을 함께 알아볼 수 있는 반응표면분석을 사용하였다. 분석 결과, 지방공무원은 정부분권에 한하여 정책 구체성 정도가 높을 때 예견적 기대가 정부신뢰를 높였다. 규범적 기대는 정책 구체성 정도가 높을 때 예견적 기대와 결합하여 정부신뢰를 높였다. 본 연구는 예견적 기대가 정부신뢰에 미치는 긍정적 영향을 다른 층위의 정부 간 관계에서 실증하고, 지방공무원의 기대와 중앙정부신뢰의 관계가 분권의 주체와 정책 구체성 정도에 따라 다를 수 있음을 보였다는 점에서 의의가 있다. When the expectations of public servants regarding the various policies announced at the inauguration of a new government increase, it can contribute to fostering trust in the government and facilitating smooth policy implementation. This study surveyed 233 civil servants from the main office of Daejeon Metropolitan City in July 2022 to examine how the predictive and normative expectations of local civil servants regarding eight key tasks of the Yoon Seok-yeol administration—designed to strengthen decentralization—affect trust in the central government. The eight tasks were categorized into four types based decentralization types, and the level of policy specificity. A responsive surface analysis was employed as the methodology, which not only assesses the independent impact of each expectation but also examines the interaction between the two types of expectations and their effect on government trust. The analysis revealed that local government employees exhibited positive impact of predictive expectations on government trust only when the policy specificity for government decentralization was high. Additionally, normative expectations were found to enhance trust in the government when combined with predictive expectations, provided the policy specificity was high. This study is significant in that it highlights the positive influence of expectations on government trust across different levels of intergovernmental relations and demonstrates that the relationship between local government officials’ expectations and trust in the central government may vary based on the types of decentralization and the degree of policy specificity.

세대간 스마트워크 효과성에 대한 인식 차이에 대한 연구: MZ세대 공무원과 기타 세대 공무원을 대상으로

김필,황성수 한국인사행정학회 2022 한국인사행정학회보 Vol.21 No.4

This study explores whether MZ officials in the public sector are making good use of smartwork and how they perceive smartwork in the public sector. We tried to empirically find out the expectations of an increase in smartwork use due to the increase in remote work in COVID-19. Two FGI surveys for the MZ generation in the public sector and a survey to 226 public officials from the Ministry of Public Administration and Security were used to conduct intergroup t-test and moderated regression analysis. As a result, both the MZ generation and other generations recognized a difference in smartwork adaptability and preference between generations, and the MZ generation recognized the difference significantly higher. The MZ generation had higher satisfaction with telecommuting and mobile authorization than other generations and recognized that smartwork contributes to faster work processes and more flexible organizational culture. There was no significant difference between generations in smartwork fairness. Smart work discretion, innovation behavior, and public service motivation showed significantly positive influence on smartwork effectiveness. In conclusion, policy implications were drawn that the higher the tendency to innovate, the higher the expectation for smartwork use, and that workplace innovation by using smartwork institutions in the public sector is possible in the future.

Comparing Government Performance Indicators: A Fuzzy-set Analysis

김필,임도빈 서울대학교행정대학원 2019 The Korean Journal of Policy Studies Vol.34 No.2

National governments rely on global performance indicators to measure where they stand and to build future strategies. However, no previous study has compiled various indices to investigate pathways to government performance. We use fuzzy-set analysis to investigate what role each of five determinants of government performance—trait competitiveness, change-oriented citizenship behavior, public service motivation, organizational identification, and corruption tolerance—play in three representative government performance indicators—“Government Effectiveness”, “Government Efficiency”, and “Throughput”. The results indicate that government performance as measured by these three indicators is commonly tied to strongly public-service-motivated employees. These three indicators are distinguished from one another with regard to the number of factors that contribute to the construction of sufficient configurations, the role of innovation-inclined factors, and the role of corruption tolerance.